Article
Parasitology
Juri Kim, Eun-Ah Park, Mee Young Shin, Soon-Jung Park
Summary: This study confirms the transcriptional activation function of GlMyb2 during encystation in Giardia lamblia. Through chromatin immunoprecipitation experiments and quantitative real-time PCR, several target genes of GlMyb2 were identified, with a detailed analysis on the promoter region of glcwp1 gene.
PARASITES & VECTORS
(2022)
Article
Parasitology
Mari Shiratori, Abhishek Patel, Richard W. Gerhold, Steven A. Sullivan, Jane M. Carlton
Summary: Recent studies have shown that Trichomonas vaginalis can establish persistent infections in the vagina, and this understudied infection can have adverse consequences such as increased risk of HIV-1 infection and cervical cancer. Further research on the spherical form of T. vaginalis called 'pseudocyst' and persistent infections is necessary to improve the diagnosis and treatment of trichomoniasis.
TRENDS IN PARASITOLOGY
(2023)
Article
Parasitology
Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Park
Summary: The study reveals the role of GlPLK in Giardia cell division, especially during cytokinesis, and its involvement in flagella formation.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Shao-Wei Huang, Zi-Qi Lin, Szu-Yu Tung, Li-Hsin Su, Chun-Che Ho, Gilbert Aaron Lee, Chin-Hung Sun
Summary: The study revealed that Giardia's MBF1 gene plays a crucial role in cyst differentiation by binding to key promoter regions of cwp1-3 and myb2 genes and interacting with transcription factors to up-regulate cwp gene expression during encystation. This positive regulation of MBF1 in Giardia suggests a functional conservation of the gene in driving cyst formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Heta P. Patel, Stefano Coppola, Wim Pomp, Umberto Aiello, Ineke Brouwer, Domenico Libri, Tineke L. Lenstra
Summary: DNA supercoiling is a major factor in gene regulation in bacteria, but its impact on transcription dynamics in eukaryotes has been unclear. Single-molecule dual-color nascent transcription imaging in budding yeast reveals that transcriptional bursting of neighboring GAL genes is coupled. This coupling requires rapid release of DNA supercoils by topoisomerases. Accumulated supercoils inhibit transcription of adjacent genes by destabilizing the binding of the transcription factor Gal4. Wild-type yeast minimizes this inhibition by maintaining sufficient levels of topoisomerases, ensuring proper gene expression of neighboring genes.
Article
Plant Sciences
Baoguang Liu, Juan Liu, Jing Yu, Zhifeng Wang, Yi Sun, Shuang Li, Ying-Chung Jimmy Lin, Vincent L. Chiang, Wei Li, Jack P. Wang
Summary: Tension wood (TW) is a specialized xylem tissue formed in response to mechanical stress in angiosperm trees. The development of TW involves the regulation of secondary cell wall genes, leading to altered wood properties. Through analysis of TF-DNA interactions and gene editing, this study identified key transcription factors involved in the regulation of wood composition under tension stress, providing insights into the coordinated regulation of metabolism, growth, and stress adaptation in trees.
Article
Medicine, Research & Experimental
Itzel Reyes-Duarte, Alexel J. Burgara-Estrella, Lorena Bustamante-Cordova, Silvia Y. Moya-Camarena, Hector Parra-Sanchez, Osiris Alvarez-Bajo, Guillermo Lopez-Cervantes, Maricela Montalvo-Corral
Summary: This study found that conjugated linoleic acid (CLA) can improve mucosal immune function, inhibit Giardia lamblia infection, and promote early antibody production, thus restoring intestinal balance after infection.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
Article
Microbiology
Murat C. Kalem, Harini Subbiah, Jay Leipheimer, Virginia E. Glazier, John C. Panepinto
Summary: Research has shown that the lack of Puf4 in Cryptococcus neoformans results in increased resistance to caspofungin, while overexpression of Puf4 leads to increased sensitivity to the drug. Additionally, Puf4 regulates multiple cell wall biosynthesis genes and affects cell wall composition. These findings reveal a potential mechanism by which caspofungin resistance and cell wall biogenesis are post-transcriptionally regulated by Puf4.
Article
Immunology
Juliette Roels, Jolien Van Hulle, Marieke Lavaert, Anna Kuchmiy, Steven Strubbe, Tom Putteman, Bart Vandekerckhove, Georges Leclercq, Filip Van Nieuwerburgh, Lena Boehme, Tom Taghon
Summary: This study investigates the regulatory dynamics of E and ID protein encoding genes during pediatric human T cell development and uncovers novel insights through cross-species and cross-developmental comparisons.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Blondy Canto-Canche, Yamily Yazmin Burgos-Canul, Deysi Chi-Chuc, Miguel Tzec-Sima, Angela Ku-Gonzalez, Ligia Brito-Argaez, Mildred Carrillo-Pech, Cesar De los Santos-Briones, Miguel Angel Canseco-Perez, Donato Luna-Moreno, Miguel J. Beltran-Garcia, Ignacio Islas-Flores
Summary: The fungal cell wall has various functions and components, including carbohydrates and different types of proteins. In this study, the cell wall of the banana pathogen Pseudocercospora fijiensis was isolated and analyzed. Atypical cell wall proteins were identified, suggesting a new class of proteins that may play a role in fungal pathogenicity.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Haley M. Amemiya, Thomas J. Goss, Taylor M. Nye, Rebecca L. Hurto, Lyle A. Simmons, Peter L. Freddolino
Summary: The study found that prokaryotes maintain chromosome structure similar to eukaryotic heterochromatin, with transcriptionally silent regions called extended protein occupancy domains (EPODs) containing metabolic pathway genes and parasitic elements. Deletion of specific nucleoid-associated proteins (NAPs) in E. coli and B. subtilis opens up chromosomal regions for RNA polymerase binding and further transcriptional regulation, preparing cells for exposure to different metabolic environments. Additionally, novel xenogeneic silencing roles were identified for the NAPs Fis and Hfq, essential for cell viability in the presence of domesticated prophages.
Article
Biochemistry & Molecular Biology
Lautaro Natali, Gabriel Luna Pizarro, Sofia Moyano, Benjamin de la Cruz-Thea, Juliana Musso, Andrea S. Ropolo, Norbert Eichner, Gunter Meister, Melina M. Musri, Constanza Feliziani, Maria C. Touz
Summary: The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. RNA sequencing analysis revealed that the exosomal-like vesicles (ElVs) of each assemblage contained distinct small RNA (sRNA) biotypes. These sRNAs may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Hiroki Sugishita, Takashi Kondo, Shinsuke Ito, Manabu Nakayama, Nayuta Yakushiji-Kaminatsui, Eiryo Kawakami, Yoko Koseki, Yasuhide Ohinata, Jafar Sharif, Mio Harachi, Neil P. Blackledge, Robert J. Klose, Haruhiko Koseki
Summary: The study indicates that PCGF1-containing PRC1 variant plays a crucial role in the downregulation of differentiation-associated genes, facilitating proper gene expression through histone modifications and recruitment of PRC2.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Xiaozhen Huang, Nan Xiao, Yupan Zou, Yue Xie, Lingli Tang, Yueqin Zhang, Yuan Yu, Yiting Li, Cao Xu
Summary: Our study reveals a novel genetic canalization mechanism enabled by heterotypic transcriptional condensates formed by paralogous protein interactions and phase separation, uncovering the molecular link between gene duplication caused intrinsic disorder region variation and robust transcriptional control of stem cell fate transition.
Article
Plant Sciences
Cesar Bernat-Silvestre, Yingxuan Ma, Kim Johnson, Alejandro Ferrando, Fernando Aniento, Maria Jesus Marcote
Summary: This study investigates the function of Arabidopsis Per1/PGAP3 genes, and finds that PGAP3A is essential for the efficient transport of GPI-anchored proteins from the ER to the plasma membrane/cell wall. The loss of PGAP3A function increases susceptibility to salt and osmotic stresses, possibly due to altered localization of GPI-APs. In addition, PGAP3B is shown to be a functional ortholog of Per1p.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Po-Jung Huang, Wei-Chen Lin, Shih-Chieh Chen, Yong-Han Lin, Chin-Hung Sun, Ping-Chiang Lyu, Petrus Tang
Article
Biochemistry & Molecular Biology
Yu-Jiao Pan, Chao-Cheng Cho, Yu-Yun Kao, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Biochemistry & Molecular Biology
Yi-Ting Wang, Yu-Jiao Pan, Chao-Cheng Cho, Bo-Chi Lin, Li-Hsin Su, Yu-Chang Huang, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2010)
Article
Biochemistry & Molecular Biology
Chao-Cheng Cho, Li-Hsin Su, Yu-Chang Huang, Yu-Jiao Pan, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Biochemistry & Molecular Biology
Li-Hsin Su, Yu-Jiao Pan, Yu-Chang Huang, Chao-Cheng Cho, Chia-Wei Chen, Shao-Wei Huang, Sheng-Fung Chuang, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Gastroenterology & Hepatology
Tzu-Ling Chen, Shin Chen, Hsiu-Wei Wu, Tsung-Chun Lee, Yen-Zhen Lu, Li-Ling Wu, Yen-Hsuan Ni, Chin-Hung Sun, Wei-Hsuan Yu, Andre G. Buret, Linda Chia-Hui Yu
Article
Infectious Diseases
Pei-Wei Chiu, Yu-Chang Huang, Yu-Jiao Pan, Chih-Hung Wang, Chin-Hung Sun
PLOS NEGLECTED TROPICAL DISEASES
(2010)
Article
Infectious Diseases
Bo-Chi Lin, Li-Hsin Su, Shih-Che Weng, Yu-Jiao Pan, Nei-Li Chan, Tsai-Kun Li, Hsin-Chih Wang, Chin-Hung Sun
PLOS NEGLECTED TROPICAL DISEASES
(2013)
Article
Multidisciplinary Sciences
Zi-Qi Lin, Soo-Wah Gan, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Chin-Hung Sun
Article
Biochemistry & Molecular Biology
Chin-Hung Sun, Shih-Che Weng, Jui-Hsuan Wu, Szu-Yu Tung, Li-Hsin Su, Meng-Hsuan Lin, Gilbert Aaron Lee
Article
Biochemistry & Molecular Biology
Shao-Wei Huang, Zi-Qi Lin, Szu-Yu Tung, Li-Hsin Su, Chun-Che Ho, Gilbert Aaron Lee, Chin-Hung Sun
Summary: The study revealed that Giardia's MBF1 gene plays a crucial role in cyst differentiation by binding to key promoter regions of cwp1-3 and myb2 genes and interacting with transcription factors to up-regulate cwp gene expression during encystation. This positive regulation of MBF1 in Giardia suggests a functional conservation of the gene in driving cyst formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jui-Hsuan Wu, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Soo-Wah Gan, Jo-Yu Liao, Chao-Cheng Cho, Bo-Chi Lin, Pei-Wei Chiu, Yu-Jiao Pan, Yu-Yun Kao, Yu-Chen Liu, Chin-Hung Sun
Summary: The study revealed that MLF, FYVE, and ATG8L play a positive role in encystation and function in protein clearance pathway, which is important for encystation and coordinated with Exosomes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Yu-Chien Chen, Szu-Yu Tung, Chia-Wei Huang, Soo-Wah Gan, Bo-Chi Lin, Chia-Wei Chen, Zi-Qi Lin, Chin-Hung Sun
Summary: This study investigated the role of the topoisomerase Spo11 in the encystation process of Giardia lamblia. The findings indicate that Spo11 acts as a positive regulator in activating encystation, interacting with transcription factors involved in cwp gene expression for cyst formation. Disruption of the spo11 gene led to decreased cwp gene expression and cyst formation, highlighting the importance of Spo11 in regulating Giardia differentiation into cysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Summary: In this study, it was found that MLF protein is expressed under certain stress conditions and is associated with specific vesicle structures. MLF plays an important role in cell survival under stress conditions, and MLF vesicles share similar stress-induced characteristics with autophagy compartments.