Article
Biochemistry & Molecular Biology
Shao-Wei Huang, Zi-Qi Lin, Szu-Yu Tung, Li-Hsin Su, Chun-Che Ho, Gilbert Aaron Lee, Chin-Hung Sun
Summary: The study revealed that Giardia's MBF1 gene plays a crucial role in cyst differentiation by binding to key promoter regions of cwp1-3 and myb2 genes and interacting with transcription factors to up-regulate cwp gene expression during encystation. This positive regulation of MBF1 in Giardia suggests a functional conservation of the gene in driving cyst formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Parasitology
Juri Kim, Eun-Ah Park, Mee Young Shin, Soon-Jung Park
Summary: This study confirms the transcriptional activation function of GlMyb2 during encystation in Giardia lamblia. Through chromatin immunoprecipitation experiments and quantitative real-time PCR, several target genes of GlMyb2 were identified, with a detailed analysis on the promoter region of glcwp1 gene.
PARASITES & VECTORS
(2022)
Article
Parasitology
Mari Shiratori, Abhishek Patel, Richard W. Gerhold, Steven A. Sullivan, Jane M. Carlton
Summary: Recent studies have shown that Trichomonas vaginalis can establish persistent infections in the vagina, and this understudied infection can have adverse consequences such as increased risk of HIV-1 infection and cervical cancer. Further research on the spherical form of T. vaginalis called 'pseudocyst' and persistent infections is necessary to improve the diagnosis and treatment of trichomoniasis.
TRENDS IN PARASITOLOGY
(2023)
Article
Parasitology
Eun-Ah Park, Juri Kim, Mee Young Shin, Soon-Jung Park
Summary: The study reveals the role of GlPLK in Giardia cell division, especially during cytokinesis, and its involvement in flagella formation.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Lautaro Natali, Gabriel Luna Pizarro, Sofia Moyano, Benjamin de la Cruz-Thea, Juliana Musso, Andrea S. Ropolo, Norbert Eichner, Gunter Meister, Melina M. Musri, Constanza Feliziani, Maria C. Touz
Summary: The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. RNA sequencing analysis revealed that the exosomal-like vesicles (ElVs) of each assemblage contained distinct small RNA (sRNA) biotypes. These sRNAs may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jui-Hsuan Wu, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Soo-Wah Gan, Jo-Yu Liao, Chao-Cheng Cho, Bo-Chi Lin, Pei-Wei Chiu, Yu-Jiao Pan, Yu-Yun Kao, Yu-Chen Liu, Chin-Hung Sun
Summary: The study revealed that MLF, FYVE, and ATG8L play a positive role in encystation and function in protein clearance pathway, which is important for encystation and coordinated with Exosomes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Multidisciplinary Sciences
Aldian Amaruddin, Jan Pieter R. Koopman, Munawir Muhammad, Kaatje Lenaerts, Hans M. H. van Eijk, Eric A. T. Brienen, Anoecim R. Geelen, Lisette van Lieshout, Sitti Wahyuni, Ed J. Kuijper, Romy D. Zwittink, Firdaus Hamid, Erliyani Sartono, Maria Yazdanbakhsh
Summary: Intestinal helminths are highly prevalent in low-SES children in Indonesia and can contribute to poor health outcomes by affecting the gut microbiome and gut barrier function. Treatment with albendazole can reduce helminth infections and improve gut permeability, but only in children without helminth infections.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Erina A. A. Balmer, Corina D. D. Wirdnam, Carmen Faso
Summary: Unconventional protein secretion (UPS) plays important roles in cell physiology, especially at the parasite-host interface. Giardia lamblia is a parasite that releases proteins without canonical secretory signals, implicating UPS. In this study, we identified a PV/PECs-associated UPS machinery and highlighted the role of the alpha-giardin protein family in PV/PECs and UPS in Giardia.
Article
Plant Sciences
Alessio Cardini, Elisa Pellegrino, Philip J. White, Barbara Mazzolai, Marco C. Mascherpa, Laura Ercoli
Summary: This study investigated the molecular mechanisms of zinc transport in alfalfa following foliar zinc applications, identifying 12 putative genes involved in zinc transport and three functional gene modules. Shoot and root zinc concentration increased with foliar zinc applications, providing insights for genetic engineering strategies to enhance crop zinc biofortification efficiency.
Article
Biochemistry & Molecular Biology
Patricia L. A. Munoz-Munoz, Rosa E. Mares-Alejandre, Samuel G. Melendez-Lopez, Marco A. Ramos-Ibarra
Summary: In this study, the structural features and functional associations of GTOR, a G. lamblia TOR-like protein, were examined using a bioinformatics approach. It was found that GTOR shares significant similarities with functional TOR kinases. Furthermore, GTOR can form multiprotein complexes such as TORC1 and TORC2. These findings provide insights into the structure-function relationship of GTOR and have potential implications for developing specific anti-giardiasis therapeutic agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Summary: In this study, it was found that MLF protein is expressed under certain stress conditions and is associated with specific vesicle structures. MLF plays an important role in cell survival under stress conditions, and MLF vesicles share similar stress-induced characteristics with autophagy compartments.
Article
Biochemistry & Molecular Biology
Yu-Chien Chen, Szu-Yu Tung, Chia-Wei Huang, Soo-Wah Gan, Bo-Chi Lin, Chia-Wei Chen, Zi-Qi Lin, Chin-Hung Sun
Summary: This study investigated the role of the topoisomerase Spo11 in the encystation process of Giardia lamblia. The findings indicate that Spo11 acts as a positive regulator in activating encystation, interacting with transcription factors involved in cwp gene expression for cyst formation. Disruption of the spo11 gene led to decreased cwp gene expression and cyst formation, highlighting the importance of Spo11 in regulating Giardia differentiation into cysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Yumeng Chen, Aibo Lin, Pei Liu, Xingjia Fan, Chuan Wu, Ni Li, Liujing Wei, Wei Wang, Dongzhi Wei
Summary: A novel transcriptional activator, ACE4, was identified to positively regulate cellulase gene expression in Trichoderma reesei by directly binding to the promoters of cellulase genes and the activator gene ace3. This discovery sheds light on the regulatory mechanism of gene expression in T. reesei and provides insights into enhancing cellulase production in industrial applications.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Sung-Min Park, Tae-Il Kang, Jae-Seon So
Summary: XBP1s is an active transcription factor that plays a critical role in regulating UPR target genes to reduce ER stress. It also controls the transcription of genes involved in lipid and glucose metabolism, as well as immune responses. Understanding the target genes and transcriptional regulatory mechanisms of XBP1s will aid in developing new therapeutic targets for various diseases.
Article
Medicine, Research & Experimental
Itzel Reyes-Duarte, Alexel J. Burgara-Estrella, Lorena Bustamante-Cordova, Silvia Y. Moya-Camarena, Hector Parra-Sanchez, Osiris Alvarez-Bajo, Guillermo Lopez-Cervantes, Maricela Montalvo-Corral
Summary: This study found that conjugated linoleic acid (CLA) can improve mucosal immune function, inhibit Giardia lamblia infection, and promote early antibody production, thus restoring intestinal balance after infection.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Po-Jung Huang, Wei-Chen Lin, Shih-Chieh Chen, Yong-Han Lin, Chin-Hung Sun, Ping-Chiang Lyu, Petrus Tang
Article
Biochemistry & Molecular Biology
Yu-Jiao Pan, Chao-Cheng Cho, Yu-Yun Kao, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2009)
Article
Biochemistry & Molecular Biology
Yi-Ting Wang, Yu-Jiao Pan, Chao-Cheng Cho, Bo-Chi Lin, Li-Hsin Su, Yu-Chang Huang, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2010)
Article
Biochemistry & Molecular Biology
Chao-Cheng Cho, Li-Hsin Su, Yu-Chang Huang, Yu-Jiao Pan, Chin-Hung Sun
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Multidisciplinary Sciences
Shen-Fung Chuang, Li-Hsin Su, Chao-Cheng Cho, Yu-Jiao Pan, Chin-Hung Sun
Article
Gastroenterology & Hepatology
Tzu-Ling Chen, Shin Chen, Hsiu-Wei Wu, Tsung-Chun Lee, Yen-Zhen Lu, Li-Ling Wu, Yen-Hsuan Ni, Chin-Hung Sun, Wei-Hsuan Yu, Andre G. Buret, Linda Chia-Hui Yu
Article
Infectious Diseases
Pei-Wei Chiu, Yu-Chang Huang, Yu-Jiao Pan, Chih-Hung Wang, Chin-Hung Sun
PLOS NEGLECTED TROPICAL DISEASES
(2010)
Article
Infectious Diseases
Bo-Chi Lin, Li-Hsin Su, Shih-Che Weng, Yu-Jiao Pan, Nei-Li Chan, Tsai-Kun Li, Hsin-Chih Wang, Chin-Hung Sun
PLOS NEGLECTED TROPICAL DISEASES
(2013)
Article
Multidisciplinary Sciences
Zi-Qi Lin, Soo-Wah Gan, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Chin-Hung Sun
Article
Biochemistry & Molecular Biology
Chin-Hung Sun, Shih-Che Weng, Jui-Hsuan Wu, Szu-Yu Tung, Li-Hsin Su, Meng-Hsuan Lin, Gilbert Aaron Lee
Article
Biochemistry & Molecular Biology
Shao-Wei Huang, Zi-Qi Lin, Szu-Yu Tung, Li-Hsin Su, Chun-Che Ho, Gilbert Aaron Lee, Chin-Hung Sun
Summary: The study revealed that Giardia's MBF1 gene plays a crucial role in cyst differentiation by binding to key promoter regions of cwp1-3 and myb2 genes and interacting with transcription factors to up-regulate cwp gene expression during encystation. This positive regulation of MBF1 in Giardia suggests a functional conservation of the gene in driving cyst formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jui-Hsuan Wu, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Soo-Wah Gan, Jo-Yu Liao, Chao-Cheng Cho, Bo-Chi Lin, Pei-Wei Chiu, Yu-Jiao Pan, Yu-Yun Kao, Yu-Chen Liu, Chin-Hung Sun
Summary: The study revealed that MLF, FYVE, and ATG8L play a positive role in encystation and function in protein clearance pathway, which is important for encystation and coordinated with Exosomes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Yu-Chien Chen, Szu-Yu Tung, Chia-Wei Huang, Soo-Wah Gan, Bo-Chi Lin, Chia-Wei Chen, Zi-Qi Lin, Chin-Hung Sun
Summary: This study investigated the role of the topoisomerase Spo11 in the encystation process of Giardia lamblia. The findings indicate that Spo11 acts as a positive regulator in activating encystation, interacting with transcription factors involved in cwp gene expression for cyst formation. Disruption of the spo11 gene led to decreased cwp gene expression and cyst formation, highlighting the importance of Spo11 in regulating Giardia differentiation into cysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Summary: In this study, it was found that MLF protein is expressed under certain stress conditions and is associated with specific vesicle structures. MLF plays an important role in cell survival under stress conditions, and MLF vesicles share similar stress-induced characteristics with autophagy compartments.