Article
Multidisciplinary Sciences
Giovanna Nalesso, Anne-Sophie Thorup, Suzanne Elizabeth Eldridge, Anna De Palma, Amanpreet Kaur, Kiran Peddireddi, Kevin Blighe, Sharmila Rana, Bryony Stott, Tonia Louise Vincent, Bethan Lynne Thomas, Jessica Bertrand, Joanna Sherwood, Antonella Fioravanti, Costantino Pitzalis, Francesco Dell'Accio
Summary: The WNT/CaMKII signaling pathway plays a crucial role in osteoarthritis by affecting the expression of markers such as HMOX1 and Sox-9. Inhibition of CaMKII may exacerbate cartilage damage and bone remodeling, while also upregulating the expression of matrix remodeling enzymes in articular chondrocytes. These findings suggest the potential therapeutic implications of targeting the WNT/CaMKII pathway in osteoarthritis.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Pablo del Cerro, Nicola M. Cook, Rik Huisman, Pierre Dangeville, Lauren E. Grubb, Clemence Marchal, Anson Ho Ching Lam, Myriam Charpentier
Summary: This study reveals the crucial role of holo-CaM2 in regulating the activity of CNGC15, which is essential for calcium oscillation frequency, early endosymbiosis signaling, and sustaining enhanced root nodule symbiosis. By increasing the affinity between holo-CaM2 and CNGC15, the expression of engineered holo-CaM2 accelerates the calcium oscillation frequency and enhances root nodule symbiosis. These findings highlight the importance of holo-CaM2 in modulating CNGC15 activity and the downstream root nodule symbiosis pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Drew M. Nassal, Nehal J. Patel, Sathya D. Unudurthi, Rebecca Shaheen, Jane Yu, Peter J. Mohler, Thomas J. Hund
Summary: The research found that CaMKII activates CF by phosphorylating beta(IV)-spectrin, leading to cardiac fibrosis. Experimental substitution of the phosphorylation site revealed that beta(IV)-S2254A can restore CF function. This study highlights the importance of beta(IV)-spectrin/CaMKII interaction in cardiac fibrotic signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Clinical Neurology
Jun Ueda, Norihito Uemura, Tomoyuki Ishimoto, Tomoyuki Taguchi, Masanori Sawamura, Etsuro Nakanishi, Masashi Ikuno, Shuichi Matsuzawa, Hodaka Yamakado, Ryosuke Takahashi
Summary: This study investigates the role of Ca2+-calmodulin-calcineurin signaling in the transmission of alpha-synuclein in Parkinson's disease. Inhibiting voltage-gated Ca2+ channels, calmodulin, and calcineurin blocks the neuronal uptake of alpha-synuclein preformed fibrils via macropinocytosis. Different subtypes of voltage-gated Ca2+ channels contribute differently to the neuronal uptake of alpha-synuclein preformed fibrils. Inhibiting calcineurin ameliorates the development of alpha-synuclein pathology in a mouse model.
MOVEMENT DISORDERS
(2023)
Article
Biochemistry & Molecular Biology
Shuhang Dai, Elisa Venturini, Saveg Yadav, Xiaoxuan Lin, Dylan Clapp, Martin Steckiewicz, Angela M. Gocher-Demske, Arthur M. Edelman, D. Grahame Hardie
Summary: This study reveals the important role of CaMKK2 in mediating EGFR signaling in cancer cells, with regulation mechanisms involving both calcium-dependent and calcium-independent pathways. Additionally, CaMKK2 regulates transcription of key genes, impacting cell survival and proliferation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Qingxi Zhang, Yin Huang, Anbiao Wu, Qingrui Duan, Peikum He, Haifeng Huang, Yuyuan Gao, Kun Nie, Qicai Liu, Lijuan Wang
Summary: We identified the pathogenic role of calcium/calmodulin-dependent serine protein kinase in Parkinson's disease, showing that it phosphorylated alpha-synuclein to activate mitochondrial calcium uniporter and increase mitochondrial calcium influx, leading to mitochondrial damage. Our findings highlight the therapeutic potential of targeting calcium/calmodulin-dependent serine protein kinase in Parkinson's disease treatment.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2023)
Article
Fisheries
Chen Huang, Jie Du, Bairu Ji, Sinan Gong, Chao Geng, Yanyang Miao, Qingchun Shen, Wei Gu, Li Wang, Qingguo Meng
Summary: EsCaMKII is highly transcribed in various tissues of E. sinensis and its over-expression promotes apoptosis and enhances resistance to S. eriocheiris infection, while RNAi knockdown results in increased pathogen copy numbers and crab mortality.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Antoni Kowalski, Cristine Betzer, Sigrid Thirup Larsen, Emil Gregersen, Estella A. Newcombe, Montana Caballero Bermejo, Viktor Wisniewski Bendtsen, Jorin Diemer, Christina Ernstsen, Shweta Jain, Alicia Espina Bou, Annette Eva Langkilde, Lene N. Nejsum, Edda Klipp, Robert Edwards, Birthe B. Kragelund, Poul Henning Jensen, Poul Nissen
Summary: This study shows that monomeric alpha-synuclein can activate plasma membrane Ca2+-ATPase (PMCA) to promote calcium clearance in neurons. The colocalization of alpha-synuclein and PMCA at neuronal synapses suggests a potential physiological function for alpha-synuclein in regulating calcium expulsion.
Article
Cardiac & Cardiovascular Systems
Andrew J. Boyle, Carl Schultz, Joseph B. Selvanayagam, Stuart Moir, Richard Kovacs, Nabil Dib, David Zlotnick, Mohammed Al-Omary, Stuart Sugito, Aravinda Selvarajah, Nicholas Collins, Grant McLachlan
Summary: This study found that treatment with NP202 for 3 months after primary PCI for anterior STEMI did not improve LV remodeling in patients with residual LV dysfunction. However, the drug demonstrated good safety and tolerability.
Article
Biochemistry & Molecular Biology
Yuna Amemiya, Nao Nakamura, Nao Ikeda, Risa Sugiyama, Chiaki Ishii, Masatoshi Maki, Hideki Shibata, Terunao Takahara
Summary: The study reveals that amino acids converge on regulating the TSC2-Rheb axis via Ca2+/calmodulin to increase mTORC1 activity and promote nascent protein synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yuichiro Hayashi, Ko Kobayakawa, Reiko Kobayakawa
Summary: Recent long-term optical imaging studies have shown that the activity levels of hippocampal neurons in familiar environments fluctuate on a daily to weekly basis. However, it is unclear whether there are any time-invariant properties in the neural representations of these cells. In this study, using miniature fluorescence microscopy, the neural activity of mouse hippocampus was measured in four different environments every 3 days. The results suggest that each cell has an inherent activity level and plays a characteristic role in the coding of space.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Parasitology
Hai-Jun Gao, Xu-Dong Sun, Yan-Ping Luo, Hua-Sheng Pang, Xing-Ming Ma, Ting Zhang, Tao Jing, Wei Hu, Yu-Juan Shen, Jian-Ping Cao
Summary: The study demonstrated that verapamil has anti-parasitic effects against Echinococcus by downregulating the expression of Ca2+/CaM-CaMKII, indicating that Ca2+/CaM-CaMKII may be a novel drug target for the treatment of echinococcosis.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Dawei Yang, Rong Rong, Rongliang Yang, Mengling You, Mengxiao Wang, Haibo Li, Dan Ji
Summary: This study elucidated the molecular mechanism of retinal cell damage caused by blue light, revealing that the CaMKII-Drp1 pathway plays a significant role in mediating this damage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hyeong-Jae Kim, Peter C. W. Lee, Jeong Hee Hong
Summary: This study reveals the enhancing effect of histamine on lamin-A/C expression in lung cancer cells. This enhancement is dependent on histamine-mediated intracellular calcium signaling and Ca/CaMKII activation. Additionally, histamine stimulation attenuates lung cancer cell motility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
M. Rezayian, F. Zarinkamar
Summary: This study investigated the effects of NO on plant metabolism, hormones, and secondary metabolites under salt stress in canola plants. Results showed that exogenous NO improved plant growth, increased antioxidant enzyme activity, promoted hormone balance, and enhanced salt stress tolerance.
Article
Critical Care Medicine
Sigal Liraz-Zaltsman, Yael Friedman-Levi, Dalia Shabashov-Stone, Galit Gincberg, Dana Atrakcy-Baranes, Mary Teena Joy, S. Thomas Carmichael, Alcino J. Silva, Esther Shohami
Summary: Inhibition of CCR5 signaling has been shown to have neuroprotective effects after traumatic brain injury, reducing lesion size, inflammation, and increasing ERK phosphorylation. Increased expression of both CCR5 and CXCR4 was observed in cortical cells 3 to 11 days post-injury, suggesting a potential therapeutic window for intervention.
JOURNAL OF NEUROTRAUMA
(2021)
Article
Psychology, Developmental
Jesminne Castricum, Joke H. M. Tulen, Walter Taal, Andre B. Rietman, Ype Elgersma
Summary: In this study, adults with NF1 performed similarly to controls in attention and motor learning tasks, with the only difference being shorter reaction times in controls. Measures of attention or motor learning were not significantly associated with reduced intellectual performance in NF1. These findings contrast with previous studies in children with NF1 and suggest potential differences in cognitive functioning between pediatric and adult NF1 populations.
JOURNAL OF ATTENTION DISORDERS
(2022)
Article
Biochemistry & Molecular Biology
Martina Proietti Onori, Linda M. C. Koene, Carmen B. Schaefer, Mark Nellist, Marcel de Brito van Velze, Zhenyu Gao, Ype Elgersma, Geeske M. van Woerden
Summary: The study utilized a mouse model expressing RHEBp.P37L in the somatosensory cortex to uncover the mechanism underlying epilepsy caused by mTOR pathway hyperactivation, revealing that enhanced axonal connectivity leads to hyperexcitability in distal neurons. Blocking axonal vesicle release from RHEBp.P37L neurons alone effectively stopped seizures and normalized the hyperexcitability of distant neurons.
Article
Genetics & Heredity
Sarah C. Borrie, Ellen Plasschaert, Zsuzsanna Callaerts-Vegh, Akihiko Yoshimura, Rudi D'Hooge, Ype Elgersma, Steven A. Kushner, Eric Legius, Hilde Brems
Summary: The study found that Spred1-/- mice exhibit changes in social and communicative behaviors, and pharmacological targeting of the Ras-MAPK pathway can reverse these behavioral phenotypes. Through research on mouse models, it was revealed that dysregulation of the Ras-MAPK signaling pathway plays a key role in mediating social behavior phenotypes in ASD mouse models.
Article
Medicine, Research & Experimental
Claudia Milazzo, Edwin J. Mientjes, Ilse Wallaard, Soren Vestergaard Rasmussen, Kamille Dumong Erichsen, Tejaswini Kakunuri, A. S. Elise van der Sman, Thomas Kremer, Meghan T. Miller, Marius C. Hoener, Ype Elgersma
Summary: Research findings demonstrate that ASO-induced reactivation of UBE3A in AS mice can lead to positive effects on AS phenotypes, such as restored sensitivity to seizures and improvements in behavior, indicating therapeutic potential.
Article
Psychiatry
Jesminne Castricum, Tom K. Birkenhager, Steven A. Kushner, Ype Elgersma, Joke H. M. Tulen
Summary: This study investigated inhibitory neurotransmission and cortical plasticity in MDD patients and controls using TMS technology, finding deficits in cortical plasticity in MDD patients. However, no significant differences in cortical inhibition were observed in depressed patients, suggesting that reduced cortical inhibition may not be a robust correlate of the pathophysiological mechanism in MDD.
FRONTIERS IN PSYCHIATRY
(2022)
Review
Genetics & Heredity
F. Isabella Zampeta, Ben Distel, Ype Elgersma, Rik Iping
Summary: This study reviews the development of Angelman syndrome research over the past 50 years using a bibliometric approach. The results reveal a shift in research focus towards genetic therapies for Angelman syndrome and provide insights into changes in funding sources, publishing journals, and international collaborations.
Article
Biochemistry & Molecular Biology
Nikhil J. Pandya, Sonja Meier, Stefka Tyanova, Marco Terrigno, Congwei Wang, A. Mattijs Punt, E. J. Mientjes, Audrey Vautheny, Ben Distel, Thomas Kremer, Ype Elgersma, Ravi Jagasia
Summary: This study investigates the mechanisms and potential therapeutic options for Angelman syndrome. Proteomics analysis reveals changes in protein pathways during different stages of development in an AS mouse model. Restoring UBE3A expression is shown to reverse these changes and provide a promising treatment option. Additionally, a novel UBE3A substrate is discovered, and a protein database is created to support future research.
MOLECULAR PSYCHIATRY
(2022)
Article
Neurosciences
Lucas Wahl, A. Mattijs Punt, Tara Arbab, Ingo Willuhn, Ype Elgersma, Aleksandra Badura
Summary: The marble burying test is commonly used to describe behaviors in mouse models of neurodevelopmental and psychiatric disorders. A new automated method using machine learning algorithms was introduced to quantify burying behavior in freely moving mice. The accuracy of the method was confirmed in three mouse models, showing consistent results with previously reported phenotypes. The study also revealed additional information about mouse activity during the test.
Article
Genetics & Heredity
Geeske M. van Woerden, Richelle Senden, Charlotte de Konink, Rossella A. Trezza, Anwar Baban, Jennifer A. Bassetti, Yolande van Bever, Lynne M. Bird, Bregje W. van Bon, Alice S. Brooks, Qiaoning Guan, Eric W. Klee, Carlo Marcelis, Joel M. Rosado, Lisa A. Schimmenti, Amy R. Shikany, Paulien A. Terhal, Kathryn Nicole Weaver, Marja W. Wessels, Hester van Wieringen, Anna C. Hurst, Catherine F. Gooch, Katharina Steindl, Pascal Joset, Anita Rauch, Marco Tartaglia, Marcello Niceta, Ype Elgersma, Serwet Demirdas
Summary: This study identifies clinical phenotypes associated with MAP3K7 gene mutations, confirming the correlation between different mutation types and phenotypes in FMD2 and CSCF, and highlighting the importance of considering MAP3K7 mutations in the differential diagnosis of cardiac defects, connective tissue disorders, and NS.
Article
Psychiatry
Joseph K. Tanas, Devante D. Kerr, Li Wang, Anika Rai, Ilse Wallaard, Ype Elgersma, Michael S. Sidorov
Summary: Angelman syndrome (AS) is a neurodevelopmental disorder that affects motor function, cognition, and sleep, among other comorbidities. Multidimensional analysis has been used to quantify the behavioral profiles of AS mice, accurately predicting their genotype and evaluating the effectiveness of treatment.
TRANSLATIONAL PSYCHIATRY
(2022)
Article
Medicine, Research & Experimental
A. Mattijs Punt, Matthew C. Judson, Michael S. Sidorov, Brittany N. Williams, Naomi S. Johnson, Sabine Belder, Dion den Hertog, Courtney R. Davis, Maximillian S. Feygin, Patrick F. Lang, Mehrnoush Aghadavoud Jolfaei, Patrick J. Curran, Wilfred F. J. van Ijcken, Ype Elgersma, Benjamin D. Philpot
Summary: Chromosome 15q11.2-q13.1 duplication syndrome (Dup15q syndrome) is a severe neurodevelopmental disorder characterized by intellectual disability, impaired motor coordination, and autism spectrum disorder. While overexpression of the UBE3A gene is presumed to be the main cause of Dup15q pathology, studies on mouse models with UBE3A overexpression have yielded conflicting results. In this study, transgenic mouse models were used to investigate the neurodevelopmental impact of Ube3a gene overdosage and found limited effects on neurodevelopment, but increased mortality when faced with seizures.
Article
Multidisciplinary Sciences
Changliang Guo, Garrett J. Blair, Megha Sehgal, Federico N. Sangiuliano Jimka, Arash Bellafard, Alcino J. Silva, Peyman Golshani, Michele A. Basso, Hugh Tad Blair, Daniel Aharoni
Summary: We have developed an open-source miniature microscope called MiniLFOV for neural imaging in freely behaving rats. It offers a large field of view and cellular resolution imaging, with the capability of deep brain and cortical imaging. The MiniLFOV has been successfully validated in both rats and head-fixed mice, and it also supports wire-free operation with a novel data acquisition expansion board. We believe that this new implementation of the UCLA Miniscope platform will greatly benefit researchers in studying brain function in freely behaving animals.
Meeting Abstract
Biochemistry & Molecular Biology
A. Aartsma-Rus, M. Lauffer, R. Collin, Y. Elgersma, W. van Roon-Mom
Article
Genetics & Heredity
Inge den Besten, Rianne F. de Jong, Amber Geerts-Haages, Hennie T. Bruggenwirth, Marije Koopmans, Alice Brooks, Ype Elgersma, Dederieke A. M. Festen, Marlies J. Valstar
Summary: This study describes the clinical phenotypes of Angelman syndrome (AS) in adults, revealing significant health issues such as constipation, reflux, visual problems, scoliosis, behavioral problems, and sleeping problems. Epilepsy was reported in 57% of adults with AS, impacting their level of functioning. A decline in mobility was noted in the majority of adults, with a minority showing microcephaly.
AMERICAN JOURNAL OF MEDICAL GENETICS PART A
(2021)