Article
Cardiac & Cardiovascular Systems
Matthew J. Wleklinski, Dmytro O. Kryshtal, Kyungsoo Kim, Shan S. Parikh, Daniel J. Blackwell, Isabelle Marty, V. Ramesh Iyer, Bjorn C. Knollmann
Summary: CASQ2-K180R causes CPVT2 through impairing dynamic buffering of Ca within the SR, which is different from other CASQ2-related forms of CPVT2.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Amy D. Hanna, Chang Seok Lee, Lyle Babcock, Hui Wang, Joseph Recio, Susan L. Hamilton
Summary: The study indicates that the myopathy associated with the D244G mutation in CASQ1 is driven by CASQ1 mislocalization and aggregation, reduced SR calcium release, leading to ER stress, increased protein aggregation, and other pathological changes in muscle cells.
Article
Neurosciences
Xianwei Zhang, Charlotte E. R. Smith, Stefano Morotti, Andrew G. Edwards, Daisuke Sato, William E. Louch, Haibo Ni, Eleonora Grandi
Summary: This study investigates the independent and interactive effects of TATS and Ca2+-handling protein remodelling on Ca2+-handling abnormality driven membrane instabilities. The results show that the impact of protein remodelling on Ca2+-driven proarrhythmic behaviour varies depending on TATS density.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Review
Physiology
Hiroaki Eshima
Summary: Obesity and diabetes can disrupt peripheral insulin resistance in skeletal muscle and lead to loss of muscle size, strength, and physical function, with contractile dysfunction being linked to impaired intracellular Ca2+ concentration regulation. Recent studies have shown that metabolic disorders affect interactions between the SR and mitochondrial networks, altering Ca2+ handling by these organelles. This review highlights the importance of understanding the impact of metabolic disorders on calcium regulation in skeletal muscle for potential therapeutic strategies.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rizwan Qaisar, Gavin Pharaoh, Shylesh Bhaskaran, Hongyang Xu, Rojina Ranjit, Jan Bian, Bumsoo Ahn, Constantin Georgescu, Jonathan D. Wren, Holly Van Remmen
Summary: The study showed that pharmacological activation of SERCA can mitigate sarcopenia phenotype in aging mice, reversing reductions in muscle mass and force generation, and preventing an increase in mitochondrial ROS production. These effects are mediated in part by enhanced cellular energetics through activation of PGC1-alpha, UCP1, HSF1, and APMK, as well as increased regenerative capacity by suppression of MEF2C and p38 MAPK signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jessica L. Braun, Allen C. T. Teng, Mia S. Geromella, Chantal R. Ryan, Rachel K. Fenech, Rebecca E. K. MacPherson, Anthony O. Gramolini, Val A. Fajardo
Summary: NNAT acts as a SERCA uncoupler by reducing Ca2+ uptake, potentially playing a role in adaptive thermogenesis.
Article
Cardiac & Cardiovascular Systems
Maren E. Arnold, Wolfgang R. Dostmann, Jody Martin, Michael J. Previs, Bradley Palmer, Martin LeWinter, Markus Meyer
Summary: This study introduces a novel biosensor that can visualize the interaction between SERCA2a and phospholamban (PLB). The biosensor combines SERCA2a, a circularly permutated green fluorescent protein, and PLB into one recombinant protein (SGP). Activation of proteinkinase A leads to phosphorylation of the PLB domain, resulting in a significant increase in fluorescence yield for real-time monitoring of SERCA2a and PLB interactions in cells.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Physiology
Stephen Chan, Sindy L. L. Kueh, John W. Morley, Stewart Head
Summary: This study aims to investigate the early effects of dystrophin deficiency on sarcoplasmic reticulum Ca2+ handling in the mdx mouse. The results suggest that the absence of dystrophin has little impact on Ca2+ handling in mdx mice, emphasizing the importance of distinguishing between Ca2+ pathology caused by dystrophin deficiency and muscle degeneration.
EXPERIMENTAL PHYSIOLOGY
(2022)
Article
Physiology
Molly E. E. Streiff, Andrea C. C. Corbin, Azmi A. A. Ahmad, Chris Hunter, Frank B. B. Sachse
Summary: Transient receptor potential canonical 1 (TRPC1) channels play a critical role in the physiological regulation of cardiac function and have implications in the development of cardiac diseases. This study reveals that TRPC1 channels are involved in the mechanosensitive modulation of sarcoplasmic reticulum Ca2+ concentration, highlighting their significance in cardiac physiology and potential as therapeutic targets for cardiac diseases.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physiology
Molly E. Streiff, Frank B. Sachse
Summary: The regulation of Ca2+ concentrations in cardiomyocytes is crucial for various physiological processes. Two types of Ca2+ currents, background Ca2+ entry and Ca2+ leak from the sarcoplasmic reticulum, play important roles in the modulation of Ca2+ transients and Ca2+ load. Our study provides quantitative insights into the individual contributions of these Ca2+ currents and supports the hypothesis that TRP channels are involved in cardiac contractility modulation.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biology
Stefania Murzilli, Matteo Serano, Laura Pietrangelo, Feliciano Protasi, Cecilia Paolini
Summary: This study investigates the role of CASQ1 in the postnatal development of muscle fibers. The absence of CASQ1 hinders the full maturation of CRUs and delays the progression of T-tubule biogenesis and organization. These findings highlight the importance of CASQ1 in the development and function of skeletal muscle fibers.
Article
Multidisciplinary Sciences
Jia-Jun Liu, Chin-Sheng Yu, Hsiao-Wei Wu, Yu-Jen Chang, Chih-Peng Lin, Chih-Hao Lu
Summary: The study developed a prediction system based on protein sequences and structures to evaluate the relationship between SAVs and cancer with an accuracy of 89.73%. By converting SAV characteristics into feature vectors using a support vector machine and genetic algorithm, the system was able to predict changes in protein stability caused by SAVs.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Sageanne Senneff, Madeleine M. Lowery
Summary: A computational model was presented to explore the effects of elevated extracellular potassium on force generation in muscle fibers. The model captured experimental observations of frequency-dependent force response to potassium accumulation, indicating that altered calcium release and phosphate accumulation work together with elevated potassium to affect force production during sustained contractions.
JOURNAL OF THEORETICAL BIOLOGY
(2021)
Article
Cell Biology
Sou Inagaki, Yoshiaki Suzuki, Keisuke Kawasaki, Rubii Kondo, Yuji Imaizumi, Hisao Yamamura
Summary: In this study, the role of mitochondrial fusion protein 2 (Mfn2) in the coupling of sarcoplasmic reticulum (SR) and mitochondria in vascular smooth muscle cells (VSMCs) was examined. It was found that Mfn2 is responsible for tethering mitochondria to SR, which regulates calcium signaling, ATP production, and cell proliferation in VSMCs.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Giampaolo Morciano, Alessandro Rimessi, Simone Patergnani, Veronica A. M. Vitto, Alberto Danese, Asrat Kahsay, Laura Palumbo, Massimo Bonora, Mariusz R. Wieckowski, Carlotta Giorgi, Paolo Pinton
Summary: Intracellular calcium signaling is a universal language shared by various biological entities inside cells, regulating physiological functions like muscle contraction through calcium channels, pumps, and exchangers. Abnormal calcium homeostasis can lead to heart diseases, but can be corrected through chemical, peptide-based, and pharmacological modulators.
PHARMACOLOGICAL RESEARCH
(2022)