Article
Biochemistry & Molecular Biology
Atsushi Morii, Tetsuya Inazu
Summary: HDAC8 is involved in embryoid body formation and neuronal differentiation in P19EC cells by regulating the canonical Hedgehog (Hh) signaling pathway.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Alice Correa Silva-Sousa, Guido Artemio Maranon-Vasquez, Maria Bernadete Sasso Stuani, Peter Proff, Kesly Mary Ribeiro Andrades, Flares Baratto-Filho, Mirian Aiko Nakane Matsumoto, Eva Paddenberg, Erika Calvano Kuechler, Christian Kirschneck
Summary: This study found that genetic variants in the BMP pathway are associated with variations in the palatal rugae pattern in humans. Specific genetic variants were linked to the quantity, length, shape, unification, and predominant shape of the palatal rugae.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Wanchen Wang, Ryo Shiraishi, Daisuke Kawauchi
Summary: The sonic hedgehog (SHH) pathway plays a crucial role in regulating the development of the central nervous system in vertebrates, and aberrant regulation can lead to neurodevelopmental diseases and brain tumors. Research on the mechanisms of SHH in the cerebellum has shed light on structural abnormalities and medulloblastoma, with current clinical trials exploring novel therapeutic approaches.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kyungjoo Cho, Hyuk Moon, Sang Hyun Seo, Simon Weonsang Ro, Beom Kyung Kim
Summary: The study showed that the sonic hedgehog signaling pathway is up-regulated in cholangiocarcinoma (CCC), and that the sonic hedgehog inhibitor vismodegib has a significant inhibitory effect on CCC cells. In vivo experiments using a murine model of CCC confirmed the anti-cancer efficacy of vismodegib, suggesting that pharmacological inhibition of sonic hedgehog signaling could be an effective molecular target therapy for CCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Iman Dilower, Asef J. Niloy, Vishnu Kumar, Ayushi Kothari, Eun Bee Lee, M. A. Karim Rumi
Summary: The hedgehog (HH) signaling pathway plays a crucial role in development and reproductive functions, involving various molecules, receptors, and transcription factors. It has significant roles in the central nervous system, reproductive organs, and skeletal development, as well as essential physiological functions in adult testes and ovaries.
Article
Multidisciplinary Sciences
Natasha T. Logsdon, Carla M. Gallo, Luciano Alves Favorito, Francisco J. Sampaio
Summary: Analyzing the biometric parameters and size of abdominal wall defect in fetuses with gastroschisis and omphaloceles revealed that there is no significant correlation between the size of the defect and the number of herniated internal organs.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Jia Ding, Hui-Yan Li, Li Zhang, Yuan Zhou, Jian Wu
Summary: This review focuses on the role and potential mechanisms of Hh signaling in liver cancer, highlighting its impact on HCC initiation, progression, drug resistance, and metastasis. It emphasizes that exploring the deep mechanisms of Hh signaling can guide individualized HCC management.
Article
Cell Biology
Dong Won Kim, Elsie Place, Kavitha Chinnaiya, Elizabeth Manning, Changyu Sun, Weina Dai, Kyoji Ohyama, Sarah Burbridge, Marysia Placzek, Seth Blackshaw, Ian Groves
Summary: This study used single-cell RNA sequencing and hybridization chain reaction to analyze the early development of the hypothalamus in chicks. It revealed that hypothalamic neuroepithelial cells are induced from prethalamic-like cells and identified different progenitor populations that give rise to specific hypothalamic cell types. The study also identified molecular mechanisms and candidate regulators involved in hypothalamic induction and subsequent development.
Review
Physiology
Liyuan Li, Xiaojin Shi, Yun Shi, Zhao Wang
Summary: This review discusses the follicular development process, focusing on the three cell types and regulatory signals involved. It highlights the roles of various classical signaling pathways and their underlying mechanisms in regulating follicle development.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhiyan Jiang, Liwen Qu, Gaofeng Cui, Guohua Zhong
Summary: Hh pathway antagonist sonidegib was found to have significant effects on the development of Drosophila larvae, causing epidermal abnormalities and decreased motility. Transcriptome analysis revealed its impact on chitin-based cuticle development. These findings shed new light on the potential use of Hh antagonists in agricultural pest management.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Cell Biology
Florian Hermans, Lara Hemeryck, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom
Summary: Tooth development relies on interactions between dental epithelium and mesenchyme, regulated by molecular pathways including Wnt, BMP, FGF, and Shh. These signaling modules intertwine during tooth development, outlining specific cell populations' development pathways.Recent studies have uncovered crucial Wnt-Shh interactions during odontogenesis, showing conservation in developing ectodermal appendages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chaohui Zhang, Yuxin Li, Jiaheng Cao, Beibei Yu, Kaiyue Zhang, Ke Li, Xinhui Xu, Zhikun Guo, Yinming Liang, Xiao Yang, Zhongzhou Yang, Yunfu Sun, Vesa Kaartinen, Keyue Ding, Jikui Wang
Summary: The study reveals that Smoothened-mediated hedgehog signaling controls posterior cardiac progenitor commitment, suggesting that Smoothened mutation may be involved in the etiology of congenital heart diseases related to the cardiac conduction system and heart valves.
Article
Biochemistry & Molecular Biology
Fei Fang, McKenzie Sup, Andrew Luzzi, Xavier Ferrer, Stavros Thomopoulos
Summary: Hh signaling plays essential roles in the development and repair of tendons and entheses, but its overactivation can lead to pathological ossification. Further research is needed to understand its distinct functions in tendons and entheses.
Article
Pediatrics
Haibao Zhang, Shan Xu, Dalin He, Xinyang Wang, Guodong Zhu
Summary: Autocrine SHH signaling was found in the developing urothelium, while paracrine SHH signaling was found in the developing smooth muscle layer, mediated by SMO, PTC-1, and GLI1 during human bladder development. The expression of SHH signaling components peaked at 12- and 23-weeks gestation, corresponding to different stages of urothelium and smooth muscle layer differentiation and development in the fetal bladder.
FRONTIERS IN PEDIATRICS
(2021)
Article
Medicine, Research & Experimental
Chung-Sung Lee, Ginny Ching-Yun Hsu, Takashi Sono, Min Lee, Aaron W. James
Summary: By loading Hh signaling agonists into liposomes and immobilizing them onto trabecular bone-mimetic scaffolds, a significant increase in Hh-mediated osteogenic differentiation and bone healing was achieved. This approach showed more favorable outcomes compared to traditional treatment methods for bone repair.
MOLECULAR PHARMACEUTICS
(2021)
Article
Veterinary Sciences
Motohito Goto, Toru Takeo, Riichi Takahashi, Naomi Nakagata
Summary: Severe immunodeficient NOG mice were efficiently produced using the superovulation technique with eCG and IAS treatment, resulting in increased oocyte production and successful fertilization and development into blastocysts and pups across different ages.
LABORATORY ANIMALS
(2021)
Article
Biochemistry & Molecular Biology
Ryuma Haraguchi, Gen Yamada, Aki Murashima, Daisuke Matsumaru, Riko Kitazawa, Sohei Kitazawa
Summary: The development of the female reproductive tract in mammals relies on the interaction between Wolffian ducts (WDs) and Mullerian ducts (MDs), regulated by Hedgehog (Hh) signaling. Deficiency of Sonic hedgehog (Shh), exclusively expressed in WDs, prevents the MD mesenchyme from affecting uterine growth. In vivo cell tracking reveals that cells responding to WD-derived Hh signal continue to contribute to the formation of various tissues in the developing female reproductive tract.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Correction
Multidisciplinary Sciences
Keito Okazaki, Hayato Anzawa, Zun Liu, Nao Ota, Hiroshi Kitamura, Yoshiaki Onodera, Md. Morshedul Alam, Daisuke Matsumaru, Takuma Suzuki, Fumiki Katsuoka, Shu Tadaka, Ikuko Motoike, Mika Watanabe, Kazuki Hayasaka, Akira Sakurada, Yoshinori Okada, Masayuki Yamamoto, Takashi Suzuki, Kengo Kinoshita, Hiroki Sekine, Hozumi Motohashi
Summary: A correction to this paper has been published and can be accessed through the provided link.
NATURE COMMUNICATIONS
(2021)
Article
Reproductive Biology
Daiki Hashimoto, Tsuyoshi Hirashima, Hisao Yamamura, Tomoya Kataoka, Kota Fujimoto, Taiju Hyuga, Atsushi Yoshiki, Kazunori Kimura, Shunsuke Kuroki, Makoto Tachibana, Kentaro Suzuki, Nobuhiko Yamamoto, Shin Morioka, Takehiko Sasaki, Gen Yamada
Summary: A novel murine corpus cavernosum (CC) explant culture system is established to analyze the penile erectile contraction/relaxation, providing valuable insights into the structural changes and physiological responses in the field of penile medicine.
BIOLOGY OF REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Daiki Hashimoto, Jose Gabriel R. Colet, Aki Murashima, Kota Fujimoto, Yuko Ueda, Kentaro Suzuki, Taiju Hyuga, Hiroaki Hemmi, Tsuneyasu Kaisho, Satoru Takahashi, Yousuke Takahama, Gen Yamada
Summary: The thymus facilitates mature T cell production through stromal microenvironment, which can be impaired by radiation and aging. MafB, a transcription factor in thymic stroma, plays important roles in thymic regeneration and its absence may impede thymic recovery and accelerate age-related thymic involution.
SCIENTIFIC REPORTS
(2021)
Article
Pediatrics
Mellissa C. Alcantara, Kentaro Suzuki, Alvin R. Acebedo, Yuki Sakamoto, Michiru Nishita, Yasuhiro Minami, Akira Kikuchi, Gen Yamada
Summary: External genitalia development in mice involves multiple processes regulated by signaling pathways, with Wnt5a playing a crucial role in both urethral tube formation and external genitalia outgrowth. Loss of Wnt5a disrupts cell polarity, leading to reduced cell proliferation and severe urethral defects, indicating its importance in coordinated cell migration during male external genitalia development.
CONGENITAL ANOMALIES
(2021)
Article
Multidisciplinary Sciences
Daiki Kajioka, Kentaro Suzuki, Shoko Matsushita, Shinjiro Hino, Tetsuya Sato, Shuji Takada, Kyoichi Isono, Toru Takeo, Mizuki Kajimoto, Naomi Nakagata, Mitsuyoshi Nakao, Mikita Suyama, Tony DeFalco, Shinichi Miyagawa, Gen Yamada
Summary: This study reveals that the genomic environment for male-biased genes is conserved and acquires androgen responsiveness in both sexes. Sp1 plays a key role in establishing transcriptional competency for male-biased genes in both sexes. Genetic manipulation of MafB disrupts male-type urethral differentiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Takahiro Onoki, Yoshihiro Izumi, Masatomo Takahashi, Shohei Murakami, Daisuke Matsumaru, Nao Ohta, Sisca Meida Wati, Nozomi Hatanaka, Fumiki Katsuoka, Mitsuharu Okutsu, Yutaka Yabe, Yoshihiro Hagiwara, Makoto Kanzaki, Takeshi Bamba, Eiji Itoi, Hozumi Motohashi
Summary: Activation of NRF2 in skeletal muscles increases slow oxidative muscle fiber type and improves exercise endurance capacity in female mice. NRF2 activation in skeletal muscles promotes communication with adipose tissues, facilitating the utilization of fatty acids as an energy source, leading to increased mitochondrial activity and efficient energy production during exercise, resulting in improved exercise endurance.
Article
Environmental Sciences
Haojia Ma, Keishi Ishida, Chenke Xu, Kyosuke Takahashi, Yu Li, Chenhao Zhang, Qiyue Kang, Yingting Jia, Wenxin Hu, Daisuke Matsumaru, Tsuyoshi Nakanishi, Jianying Hu
Summary: The study found that TPhP delayed puberty timing in mice and affected ovarian function, possibly by inhibiting estrogen receptor signaling. After 40 days of exposure to TPhP, a significant decrease in the number of follicles was observed in mice, indicating a decline in ovarian reserve.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Erina Shiraishi, Keishi Ishida, Daisuke Matsumaru, Akiko Ido, Youhei Hiromori, Hisamitsu Nagase, Tsuyoshi Nakanishi
Summary: Research indicates that Brazilian green propolis may act as a skin sensitizer with moderate sensitivity. The effective concentration is 1-2% and can be safely used.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mingyue Zhao, Shohei Murakami, Daisuke Matsumaru, Takeshi Kawauchi, Yo-Ichi Nabeshima, Hozumi Motohashi
Summary: Oxidative stress is a major cause of age-related decline in cells and tissues, and the KEAP1-NRF2 system plays a key role in regulating redox balance. Alpha-Klotho, a protein associated with aging suppression, has been found to have antioxidant function. NRF2 activation can counteract aging-related phenotypes caused by alpha-Klotho deficiency by reducing oxidative stress.
JOURNAL OF BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Ana M. F. Capitao, Monica Lopes-Marques, Ines Pascoa, S. B. Sainath, Youhei Hiromori, Daisuke Matsumaru, Tsuyoshi Nakanishi, Raquel Ruivo, Miguel M. Santos, L. Filipe C. Castro
Summary: Environmental chemicals have been reported to significantly disrupt the endocrine and metabolic systems of multiple animal species. This study provides the first functional analysis of PPAR in a mollusk species, finding that TBT and TPT act as antagonists of PPAR/RXR and highlighting the importance of tyrosine 277 in the response to TBT.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Biochemistry & Molecular Biology
Daisuke Matsumaru, Hozumi Motohashi
Summary: Aging is a complex process influenced by various environmental factors, with oxidative stress playing a significant role in accelerating aging. The KEAP1-NRF2 system serves as a critical defense mechanism against oxidative stress, contributing to the prevention and mitigation of physiological aging and aging-related diseases. Understanding and controlling stress-induced damage may offer insights into extending healthspan and treating aging-related conditions.
Article
Toxicology
Pinyapach Dungkokkruad, Shunsuke Tomita, Youhei Hiromori, Keishi Ishida, Daisuke Matsumaru, Kyoko Mekada, Hisamitsu Nagase, Keiichi Tanaka, Tsuyoshi Nakanishi
Summary: This study demonstrates that Health Carbon (HC) not only retains the ability of activated charcoal (AC) to enhance fecal excretion of TCDD but also reduces some of the side effects of AC.
JOURNAL OF TOXICOLOGICAL SCIENCES
(2021)
Article
Obstetrics & Gynecology
Daiki Hashimoto, Mizuki Kajimoto, Yuko Ueda, Taiju Hyuga, Kota Fujimoto, Saaya Inoue, Kentaro Suzuki, Tomoya Kataoka, Kazunori Kimura, Gen Yamada
Summary: Sinusoids are prominently distributed in the outer region of corpus cavernosum (CC), with a possibility of ectopic chondrogenesis induced by alteration of SOX9/Notch signaling with aging. In aged CC samples, histological analyses revealed the presence of ectopic chondrocytes associated with upregulated expression of SOX9 and downregulated expression of RBPJK.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2021)