Article
Dermatology
Gabrielle Notorgiacomo, Justin Klug, Scott Rapp, Steven T. Boyce, Stacey C. Schutte
Summary: This study designed and validated a bioreactor to evaluate the potential benefits of negative pressure wound therapy on skin grafts and engineered skin substitutes. The results showed that normal skin could withstand greater negative pressure, while engineered skin exhibited damage at the epidermal-dermal junction after negative pressure application. Negative pressure had a certain effect on inhibiting apoptosis in engineered skin, and it also affected the levels of vascular endothelial growth factor.
INTERNATIONAL WOUND JOURNAL
(2022)
Article
Medicine, General & Internal
Iwona Niedzielska, Lukasz Obszynski, Michal Bak, Damian Niedzielski
Summary: This study evaluated the benefits of full-thickness skin grafts in the reconstruction of midface defects after maxillectomy. The results showed that full-thickness skin grafts contributed to patient's convalescence and reduced postoperative pain, making them a good alternative for patients who are not eligible for microsurgical treatment.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Anatomy & Morphology
Muhammad Fahad Amin, Muhammad Sarmad Zubair, Muhammad Ammar
Summary: Wound healing is a complex process involving various components like extracellular matrix, blood vessels, proteases, cytokines, and chemokines. Despite numerous studies and the development of various wound healing products, poor wound healing still leads to significant morbidity and mortality incidents. Understanding the effects of topical applications of therapeutic options, such as thyroxine, is crucial for promoting fast wound healing.
Letter
Dermatology
Giacomo Santandrea, Riccardo Valli, Moira Ragazzi, Fabio Castagnetti, Michela Lai, Caterina Longo, Simonetta Piana
Summary: The study aimed to investigate the role of PRAME in reducing the risk of underestimating tumor margins in acral melanomas recurring on skin grafts.
BRITISH JOURNAL OF DERMATOLOGY
(2022)
Review
Biology
Kerim Anlas, Vikas Trivedi
Summary: The establishment of the metazoan body plan during early embryogenesis is controlled by evolutionarily conserved gene networks, with body axes generally remaining identical between phyla. Utilizing ex vivo and in vitro embryo-like systems can reveal evolutionarily conserved developmental modes and the role of external signals in shaping the early embryo.
Article
Multidisciplinary Sciences
Jake Turley, Isaac V. Chenchiah, Tanniemola B. Liverpool, Helen Weavers, Paul Martin
Summary: Wound healing is a normal physiological process that is often overlooked until complications arise. Clinicians and scientists have collaborated to understand the cellular and molecular mechanisms underlying healthy wound repair in order to develop therapeutic treatments for pathological healing. Recently, mathematicians and physicists have also joined the effort, using mathematical modeling and biological experimentation to provide new insights.
Article
Polymer Science
Anna A. Dokuchaeva, Aleksandra B. Mochalova, Tatyana P. Timchenko, Kseniya S. Podolskaya, Oxana A. Pashkovskaya, Elena V. Karpova, Ilya A. Ivanov, Natalya A. Filatova, Irina Yu Zhuravleva
Summary: This study observed the hemodynamic and structural properties of electrospun, monolayered poly-epsilon-caprolactone (PCL) grafts in an in vivo experiment using a rat aorta replacement model. The results showed that PCL grafts maintained patency without stenosis or thrombosis throughout the follow-up period. The advantages of PCL scaffolds include excellent endothelialization and good surgical outcome, while the disadvantages include slow biodegradation, ineffective cellularization, and risks for mineralization and intimal hyperplasia.
Article
Pharmacology & Pharmacy
M. S. Jessica E. Neil, Jon D. Lenn, Marc B. Brown, Adrian C. Williams
Summary: This article develops a human ex vivo skin culture model for studying the pathophysiology of inflammatory dermatoses and preclinical testing of potential therapeutic methods. The model retains tissue integrity and ability to induce inflammatory gene expression, and can mimic clinical data from inflammatory patients. Through drug challenges, the model accurately regulates inflammatory biomarkers, demonstrating its effectiveness and utility.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Medicine, Research & Experimental
Hidetaka Hara, Jeremy B. Foote, Christophe Hansen-Estruch, Mohamed H. Bikhet, Huy Q. Nguyen, Mariyam Javed, Max Oscherwitz, Dalis E. Collins, David Ayares, Takayuki Yamamoto, Timothy W. King, David K. C. Cooper
Summary: Genetically modified pig skin xenografts can survive as long as monkey skin allografts, making them a realistic clinical option for temporary treatment of burns. The presence of anti-pig antibodies in monkeys with pig skin grafts does not have detrimental effects on subsequent monkey skin allografts.
XENOTRANSPLANTATION
(2023)
Review
Microbiology
Paola Savoia, Barbara Azzimonti, Roberta Rolla, Elisa Zavattaro
Summary: The skin and gut host various microorganisms that interact with the immune system and affect immune tolerance and inflammation. Changes in the skin microbiota have been observed in skin diseases, including skin cancer. Modulating the microbiota could be a potential therapeutic option for advanced skin cancers.
Article
Dermatology
Mohammadreza (Sam) Pakyari, Reza B. Jalili, Ruhangiz Taghi Kilani, Nafise Amiri, Erin Brown, Aziz Ghahary
Summary: The study confirmed that the liquid dermal scaffold MeshFill has a high efficacy in the healing process of chronic wounds, effectively filling tunnels and cavities, accelerating wound closure, and being similar to normal skin anatomy, supporting cell growth.
EXPERIMENTAL DERMATOLOGY
(2022)
Review
Medicine, General & Internal
Philip Dueppers, Roland Bozalka, Reinhard Kopp, Anna-Leonie Menges, Benedikt Reutersberg, Claudia Schrimpf, Francisco Jose Moreno Rivero, Alexander Zimmermann
Summary: This article presents the initial experience with intact fish skin grafts (iFSGs) in treating leg wounds after necrotizing fasciitis (NF) and reviews the current clinical use of iFSGs. The authors observed excellent wound granulation and pain relief in the patients treated with iFSGs. They suggest that iFSGs have the potential to play a significant role in the future treatment of NF, but further high-quality studies are needed to confirm these results.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jee-hyun Hwang, Haengdueng Jeong, Nahyun Lee, Sumin Hur, Nakyum Lee, Jeong Jun Han, Hye Won Jang, Wang Keun Choi, Ki Taek Nam, Kyung-Min Lim
Summary: The study found that the ex vivo live full-thickness porcine skin model is an excellent alternative to animal experiments for research on the safety and efficacy of cosmetic ingredients. Lactobionic acid showed the best improvement in skin barrier function, and the model could measure TEWL, confirming the significant skin barrier-protective effects of PHAs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Zhen Li, Sumin Gu, Yumeng Quan, Kulandaiappan Varadaraj, Jean X. Jiang
Summary: Using recombinant retroviruses containing pathogenic genes for congenital cataracts, injected into embryonic chick lenses, closely mimicked the process of cataract formation and provided a reliable in vivo system for studying the development and underlying mechanisms of cataracts and other genetic diseases.
COMMUNICATIONS BIOLOGY
(2021)
Article
Dermatology
H. Post, J. E. Hundt, G. Zhang, R. Depping, C. Rose, E. A. Langan, R. Paus
Summary: This study used experimentally wounded healthy full-thickness skin under pathological conditions to mimic key parameters of impaired human skin wound healing, finding that under these conditions, dermal-epidermal split formation and dyskeratosis were prominent, epidermal reepithelialisation was significantly impaired, and markers of intracutaneous angiogenesis were reduced. Thyroxine administration was found to rescue re-epithelialisation and promote epidermal keratinocyte proliferation and angiogenesis under pathological conditions.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2021)
Review
Cell & Tissue Engineering
Isabelle Schneider, Maurizio Calcagni, Johanna Buschmann
Summary: Adipose tissue is an easily accessible source of stem cells, and recent studies have been exploring their therapeutic potential in wound healing and treatment of skin diseases. This narrative review provides an overview of the latest research in these areas and focuses on the different applications of adipose-derived stem cells.
Article
Surgery
Marco Guidi, Matteo Guzzini, Carolina Civitenga, Riccardo Maria Lanzetti, Bong-Sung Kim, Inga Swantje Besmens, Martin Riegger, Stefano Lucchina, Maurizio Calcagni, Dario Perugia
Summary: This study evaluates the outcomes of using nonvascularized iliac crest grafts or vascularized bone grafts for the treatment of nonunion of long bones. The results show that vascularized bone grafts have higher success rates and medial femoral condyle corticoperiosteal flaps seem to produce better clinical and radiological outcomes compared to fibula flaps.
JOURNAL OF HAND AND MICROSURGERY
(2023)
Article
Critical Care Medicine
Lisanne Grunherz, Ramona Michienzi, Claudine Schaller, Daniel Rittirsch, Semra Uyulmaz, Bong - Sung Kim, Pietro Giovanoli, Nicole Lindenblatt
Summary: It has been shown that Nexobrid(R) is a safe and effective method for debriding deep burns and reducing the need for surgical escharotomy. However, there is limited evidence for its use in larger body areas. This retrospective study found that the use of Nexobrid(R) for preventive decompression was less common but had a lower mortality rate compared to surgical escharotomy.
Review
Biochemistry & Molecular Biology
Iris Miescher, Julia Rieber, Maurizio Calcagni, Johanna Buschmann
Summary: Tendon injuries often heal slowly, resulting in fibrovascular scar formation and reduced mechanical properties. Insulin-like growth factor-1 (IGF-1) has been studied for its ability to promote tendon healing through cell proliferation, DNA synthesis, and matrix production, particularly collagen I. However, more in vivo studies are needed to facilitate the clinical application of IGF-1 in orthopedic clinics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Inga S. Besmens, Charlotte Shahrdar, Duveken B. Y. Fontein, Sophie Knipper, Pietro Giovanoli, Nicole Lindenblatt
Summary: This study analyzed the factors affecting the outcome and need for revision rhinoplasty in patients who underwent closed reduction for nasal bone fracture. The results showed that patients with an additional septum fracture were more likely to require revision rhinoplasty. The risk of needing open revision rhinoplasty after fracture healing increased for patients complaining of airway obstruction at the time of cast removal after closed reduction.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2023)
Article
Surgery
Anna Burger, Marcus Kiehlmann, Lisanne Gruenherz, Epameinondas Gousopoulos, Michael Sohn, Nicole Lindenblatt, Pietro Giovanoli, Ulrich Michael Rieger
Summary: This study aimed to evaluate the use of a dermal template called MatriDerm (R) in reducing complications and hospital stay in phalloplasty surgeries. The use of MatriDerm (R) and split-thickness skin graft significantly reduced the rate of complications compared to full-thickness skin graft. The forearm and groin areas were both affected by complications in the full-thickness skin graft group.
INDIAN JOURNAL OF SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Julia Rieber, Gabriella Meier-Burgisser, Iris Miescher, Franz E. Weber, Petra Wolint, Yang Yao, Esteban Ongini, Athanasios Milionis, Jess G. Snedeker, Maurizio Calcagni, Johanna Buschmann
Summary: Tendon injuries can result in limited range of motion and poor biomechanical outcomes. A novel three-layer tube based on polymer DegraPol with incorporated IGF-1 was developed using emulsion electrospinning. The IGF-1-containing tubes exhibited sustained release of the growth factor and showed bioactivity by upregulating gene expression. They also demonstrated superior mechanical properties compared to pure DP tubes. These tubes may help accelerate the healing process and reduce adhesion formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Surgery
Anna Wang, Lisanne Grunherz, Ilaria V. De Martini, Mauro Vasella, Pietro Giovanoli, Nicole Lindenblatt
Summary: This study compared the outcomes and satisfaction of autologous lipofilling performed during active and stable phases of PHA and LS. The results showed similar treatment outcomes in both groups, with social factors having the greatest negative impact on quality of life.
Article
Surgery
Lisanne Grunherz, Carlotta Barbon, Epameinondas Gousopoulos, Semra Uyulmaz, Pietro Giovanoli, Nicole Lindenblatt
Summary: Patients with upper and lower limb lymphedema can experience various physical and psychological symptoms that reduce their quality of life. Lymphatic reconstructive surgery has been proven to be beneficial for these patients, but solely relying on volume reduction measurements may not accurately evaluate postoperative outcomes. This study found that patients reported significant improvements in various aspects, particularly in physical function, symptoms, and psychological well-being. There was no correlation between the extent of volume reduction and improvement in quality of life, highlighting the importance of using standardized patient-reported outcome measures to evaluate the benefits of lymphatic reconstructive surgery.
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2023)
Article
Rehabilitation
Vera Beckmann-Fries, Maurizio Calcagni, Lorena Schrepfer, Alexandre Kaempfen, Esther Vogelin, Bernadette Tobler-Ammann
Summary: This study analyzed the evolution of pain after flexor tendon repair, examined the differences in pain levels between patients with and without digital nerve injury, and evaluated the relationship between pain, nerve injury, and clinical outcomes such as TAM, DASH scores, and patient satisfaction.
Article
Engineering, Biomedical
Adam Wahlsten, Alberto Stracuzzi, Ines Luchtefeld, Gaetana Restivo, Nicole Lindenblatt, Costanza Giampietro, Alexander E. Ehret, Edoardo Mazza
Summary: The mechanical properties of the skin have been studied at both tissue and cellular length scales. The tension-compression nonlinearity of the collagen fiber network and the properties of the ground matrix contribute to the elasticity of the skin. Microscale indentation measurements do not engage the fiber network, and therefore cannot accurately predict the behavior of dermal fibroblasts.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Johannes Durig, Maurizio Calcagni, Johanna Buschmann
Summary: The aim of this paper is to review the literature on the influence of transition metals on angiogenesis. Angiogenesis is the process of blood vessel formation, which is crucial for human development. Studies have shown that transition metals have both pro- and anti-angiogenic properties. However, there are still many unanswered questions and uninvestigated transition metals in this field. Investigating these remaining elements and further analyzing the established effects is of great interest.
MATERIALS TODAY BIO
(2023)
Article
Surgery
Andrea Weinzierl, Carlotta Barbon, Epameinondas Gousopoulos, Donata von Reibnitz, Pietro Giovanoli, Lisanne Grunherz, Nicole Lindenblatt
Summary: Micro- and supermicrosurgeries have become standard techniques for lymphatic reconstruction. Robotic-assisted surgery is a new approach that allows for better accessibility of deep anatomical regions, although it sacrifices operating speed. In a case series of 8 patients, robot-assisted lymphatic microsurgery showed clear benefits in surgeon positioning and access to recipient vessels near the thoracic wall.
Article
Surgery
Lisanne Gruenherz, Epameinondas Gousopoulos, Carlotta Barbon, Semra Uyulmaz, Pietro Giovanoli, Nicole Lindenblatt
Summary: In recent years, surgical robotic systems specifically designed for microsurgery have expanded the use of robotic-assisted surgery in plastic reconstructive procedures. Currently, there are two microsurgical robotic systems available for reconstructive plastic surgery. These systems incorporate tremor reduction and motion scaling technologies to enhance surgical precision and dexterity. At the University Hospital Zurich, the Symani Surgical System has been successfully used for various microsurgical and supermicrosurgical procedures, including autologous breast reconstruction, nerve transfer, and reconstructive lymphatic surgery. Despite technical challenges like the lack of haptic feedback, the benefits outweigh the drawbacks for trained and skilled microsurgeons, offering smaller incisions and improved surgical precision for deep anatomical structures.
Article
Medicine, Research & Experimental
Pablo Pfister, Pedro David Wendel-Garcia, Isabelle Meneau, Mauro Vasella, Jennifer Ashley Watson, Philipp Buehler, Daniel Rittirsch, Nicole Lindenblatt, Bong-Sung Kim
Summary: Burn wounds present challenges in medical treatment, with the use of amniotic membranes as a biological dressing showing promise for faster healing and improved scar quality. This study aims to investigate the efficacy of using human amniotic membranes in treating burn wounds, comparing them to allogenic skin grafts in a randomized-controlled trial involving 30 patients.
CONTEMPORARY CLINICAL TRIALS COMMUNICATIONS
(2023)