Article
Microbiology
Nadja A. Henke, Irene Krahn, Volker F. Wendisch
Summary: This study successfully achieved strong and overexpression of target genes by designing new expression vectors and combining two strategies, providing new possibilities for the development of biotechnology applications.
Article
Biotechnology & Applied Microbiology
Tao Su, Chengchuan Che, Jiyu Han, Yuying Zhao, Zihan Zhang, Guangdi An, Meiru Si, Can Chen
Summary: The study reveals that AtsR is a redox regulator that senses oxidative stress and can directly repress and indirectly regulate the expression of related genes.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Yang Liu, Wenzhi Yang, Tao Su, Chengchuan Che, Guizhi Li, Can Chen, Meiru Si
Summary: This study revealed that CssR in C. glutamicum mediates stress response through ligand-induced conformational changes of the protein, rather than cysteine oxidation-based thiol modification. CssR negatively controls the expression of the ncgl1576-ncgl1577 operon, its structural gene, and/or redox homeostasis-related genes, showing its crucial role in stress response.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Nan Lu, Chenglin Zhang, Wenjie Zhang, Haoran Xu, Yuhong Li, Minhua Wei, Jing Meng, Yan Meng, Junzhe Wang, Ning Chen
Summary: In this study, a myo-inositol-inducible expression vector pMI-4 was constructed for stable replication in Corynebacterium glutamicum. The vector showed potential applications in metabolic engineering and industrial fermentation by significantly accumulating 5-aminolevulinic acid through overexpression of hemA(M) and hemL.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qiaoyun Zhu, Qi Liu, Chaoying Yao, Yuanxing Zhang, Menghao Cai
Summary: In this study, yeast transcriptional device libraries were developed for precise synthesis of value-added chemicals from methanol. The transcriptional devices showed good activity and expression strength in a methylotrophic yeast. By selecting and optimizing the devices, the production of chemicals from methanol can be significantly increased.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemical Research Methods
Yanting Duan, Weiji Zhai, Weijia Liu, Xiaomei Zhang, Jin-Song Shi, Xiaojuan Zhang, Zhenghong Xu
Summary: In this research, 17 core promoters and 29 RBSs in Corynebacterium glutamicum were characterized, showing significant differences in transcriptional and translational activity. The study revealed that RBSs contribute more to gene expression than promoters, but their efficacy is influenced by gene context, indicating poor orthogonality.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Tobias Busche, Hana Dostalova, Lenka Rucka, Jiri Holatko, Ivan Barvik, Vaclav Stepanek, Miroslav Patek, Joern Kalinowski
Summary: The sigma H and sigma E subunits of Corynebacterium glutamicum RNA polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) sigma factors. While the genes of the C. glutamicum sigma(Eta) regulon involved in heat and oxidative stress response have been studied, the genes of the sigma(E) regulon, involved in cell surface stress response, have not been explored until now. Differential gene expression analysis revealed a significant overlap between the sigma(Eta) and sigma(E) regulons, and identified genes involved in protein quality control, regulation of Clp proteases, and membrane integrity maintenance.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Gi Yeon Kim, Jinyoung Kim, Geunyung Park, Hyeon Jin Kim, Jina Yang, Sang Woo Seo
Summary: Corynebacterium glutamicum is a promising microorganism for industrial production of amino acids, fuels, and value-added chemicals. It has been widely used in industrial microbiology and biotechnology due to its genome sequence and manipulation techniques. This review provides an overview of genomic manipulation technologies and gene expression tools for transcription and translation control in C. glutamicum, and discusses the challenges and applicable tools for future advancements.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Philipp Moritz Fricke, Martha Luerkens, Max Huennefeld, Christiane K. Sonntag, Michael Bott, Mehdi D. Davari, Tino Polen
Summary: The expression performance of the pBBR1MCS-5-based TetR-P-tet system in G. oxydans is highly suitable, with inducible ratios of up to 3500-fold and tunability by varying anhydrotetracycline concentration. The LacI-dependent expression system from P-lacUV5 in G. oxydans exhibited some non-induced basal expression and was only tunable up to 40-fold induction by IPTG. Protein-DNA binding simulations suggested a potential link between decreased DNA binding of LacI at low pH and leakiness in LacI-dependent systems tested in AAB.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Chunjun Zhan, Namil Lee, Guangxu Lan, Qingyun Dan, Aidan Cowan, Zilong Wang, Edward E. K. Baidoo, Ramu Kakumanu, Bridget Luckie, Rita C. Kuo, Joshua McCauley, Yuzhong Liu, Luis Valencia, Robert W. Haushalter, Jay D. Keasling
Summary: Corynebacterium glutamicum is a promising host for producing valuable polyketides, but propionate addition inhibits its growth. This study identifies the accumulation of propionyl-CoA and methylmalonyl-CoA as the cause of growth inhibition and demonstrates that it can be relieved by introducing methylmalonyl-CoA-dependent polyketide synthases. Laboratory evolution is used to increase polyketide production by targeting mutations in germicidin synthase and citrate synthase. These findings highlight the importance and potential of C. glutamicum in polyketide production.
Article
Biotechnology & Applied Microbiology
Puying Zhao, Meng Zhang, Xiaofu Wan, Peiling Geng, Hairong Xiong, Xiaomin Hu
Summary: This study aims to explore the potential aminopeptidases of Lysinibacillus sphaericus. Through heterologous expression, a high hydrolysis ability was found in Amp0279, which belongs to the novel Co2+-dependent aminopeptidase of the M29 family. The optimal conditions of Amp0279 were determined, and its expression was observed in different stages.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jingbai Wen, Jie Bao
Summary: By secreting glutamate decarboxylase, Corynebacterium glutamicum achieved efficient production of γ-aminobutyric acid, significantly increasing the yield.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Melanie Mindt, Lenny Ferrer, Dirk Bosch, Katarina Cankar, Volker F. Wendisch
Summary: Indole has gained increasing interest in the flavor and fragrance industry due to its distinct floral odor. However, the current production methods are non-sustainable and unprofitable. To offer an alternative approach, biosynthesis of indole has been studied, including glucose-based production using genetically-engineered strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Taehee Han, Sang Yup Lee
Summary: The biosynthesis of Valerolactam (VL) was achieved by metabolically engineered Corynebacterium glutamicum, which overproduces L-lysine. This provides a green and sustainable method for the production of precursor chemicals for nylon-5 and nylon 6,5.
METABOLIC ENGINEERING
(2023)
Article
Microbiology
Jingwen Huang, Jiuzhou Chen, Yu Wang, Tuo Shi, Xiaomeng Ni, Wei Pu, Jiao Liu, Yingyu Zhou, Ningyun Cai, Shuangyan Han, Ping Zheng, Jibin Sun
Summary: By developing a hyperosmotic stress inducible gene expression system, successful gene auto-regulation was achieved in C. glutamicum, significantly increasing lysine production.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Johannes Hemmerich, Peter Rohe, Britta Kleine, Sarah Jurischka, Wolfgang Wiechert, Roland Freudl, Marco Oldiges
MICROBIAL CELL FACTORIES
(2016)
Article
Biochemistry & Molecular Biology
Agnes Ulfig, Julia Froebel, Frank Lausberg, Anne-Sophie Bluemmel, Anna Katharina Heide, Matthias Mueller, Roland Freudl
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biotechnology & Applied Microbiology
Roland Freudl
JOURNAL OF BIOTECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Britta Kleine, Ava Chattopadhyay, Tino Polen, Daniela Pinto, Thorsten Mascher, Michael Bott, Melanie Brocker, Roland Freudl
MOLECULAR MICROBIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Agnes Ulfig, Roland Freudl
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Biotechnology & Applied Microbiology
Roland Freudl
MICROBIAL CELL FACTORIES
(2018)
Article
Multidisciplinary Sciences
Dan Oertel, Sabrina Schmitz, Roland Freudl
Article
Microbiology
Roland Freudl
RESEARCH IN MICROBIOLOGY
(2013)
Article
Multidisciplinary Sciences
Rene Steffen Hauer, Roland Freudl, Julia Dittmar, Mario Jakob, Ralf Bernd Kloesgen
SCIENTIFIC REPORTS
(2017)
Article
Biotechnology & Applied Microbiology
Johannes Hemmerich, Matthias Moch, Sarah Jurischka, Wolfgang Wiechert, Roland Freudl, Marco Oldiges
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Sarah Jurischka, Astrid Bida, Doris Dohmen-Olma, Britta Kleine, Janko Potzkei, Stephan Binder, Georg Schaumann, Patrick J. Bakkes, Roland Freudl
MICROBIAL CELL FACTORIES
(2020)
Article
Microbiology
Patrick J. Bakkes, Patrick Lenz, Carolin Mueller, Astrid Bida, Doris Dohmen-Olma, Andreas Knapp, Marco Oldiges, Karl-Erich Jaeger, Roland Freudl
Summary: The development of a new reporter strain with fluorescence protein expression in C. glutamicum has enabled the quantification of protein secretion and optimization of cutinase secretion from Fusarium solani pisi through strategies involving signal peptide library and mutant library. This biosensor-based approach not only significantly increased the secretion efficiency of cutinase, but also improved the specific activity by identifying key mutations in the signal peptide and enzyme structure.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Carolin Mueller, Patrick J. Bakkes, Patrick Lenz, Vera Waffenschmidt, Laura M. Helleckes, Karl-Erich Jaeger, Wolfgang Wiechert, Andreas Knapp, Roland Freudl, Marco Oldiges
Summary: Automated workflows were developed for heterologous production of cutinase in Corynebacterium glutamicum. The best performing signal peptides for cutinase secretion were identified using enzymatic activity and split GFP assay.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Angela Kranz, Tino Polen, Christian Kotulla, Annette Arndt, Graziella Bosco, Michael Bussmann, Ava Chattopadhyay, Annette Cramer, Cedric-Farhad Davoudi, Ursula Degner, Ramon Diesveld, Raphael Freiherr von Boeselager, Kim Gaertner, Cornelia Gaetgens, Tobias Georgi, Christian Geraths, Sabine Haas, Antonia Heyer, Max Huennefeld, Takeru Ishige, Armin Kabus, Nicolai Kallscheuer, Larissa Kever, Simon Klaffl, Britta Kleine, Martina Kocan, Abigail Koch-Koerfges, Kim J. Kraxner, Andreas Krug, Aileen Krueger, Andreas Kueberl, Mohamed Labib, Christian Lange, Christina Mack, Tomoya Maeda, Regina Mahr, Stephan Majda, Andrea Michel, Xenia Morosov, Olga Mueller, Arun M. Nanda, Jens Nickel, Jennifer Pahlke, Eugen Pfeifer, Laura Platzen, Paul Ramp, Doris Rittmann, Steffen Schaffer, Sandra Scheele, Stephanie Spelberg, Julia Schulte, Jens-Eric Schweitzer, Georg Sindelar, Ulrike Sorger-Herrmann, Markus Spelberg, Corinna Stansen, Apilaasha Tharmasothirajan, Jan van Ooyen, Philana van Summeren-Wesenhagen, Michael Vogt, Sabrina Witthoff, Lingfeng Zhu, Bernhard J. Eikmanns, Marco Oldiges, Georg Schaumann, Meike Baumgart, Melanie Brocker, Lothar Eggeling, Roland Freudl, Julia Frunzke, Jan Marienhagen, Volker F. Wendisch, Michael Bott
Summary: Corynebacterium glutamicum is a major host for amino acid production and has been extensively studied in microbial biotechnology. This study presents a large compendium of transcriptional profiles, allowing researchers to investigate gene expression changes under different environmental conditions or genetic modifications. The compendium provides valuable insights into the understanding of C. glutamicum as a microbial cell factory.
Article
Genetics & Heredity
Patrick J. Bakkes, Paul Ramp, Astrid Bida, Doris Dohmen-Olma, Michael Bott, Roland Freudl
