期刊
PLASMID
卷 68, 期 2, 页码 142-147出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2012.05.001
关键词
Corynebacterium glutamicum; Expression vector; Anhydrotetracycline; TetR; Promoter
Here we report on the construction of a tetracycline inducible expression vector that allows a tightly regulable gene expression in Corynebacterium glutamicum which is used in industry for production of small molecules such as amino acids. Using the green fluorescent protein (GFP) as a reporter protein we show that this vector, named pCLTON1, is characterized by tight repression under non-induced conditions as compared to a conventional IPTG inducible expression vector, and that it allows gradual GFP synthesis upon gradual increase of anhydrotetracycline addition. (C) 2012 Elsevier Inc. All rights reserved.
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