Article
Genetics & Heredity
Shuai Zhang, Song Gao, Yu Chen, Sha Xu, Shiqin Yu, Jingwen Zhou
Summary: The leaves of Ampelopsis grossedentata are rich in dihydromyricetin, which has many potential applications. In this study, hydroxylase genes involved in the biosynthesis of dihydromyricetin were identified and characterized. The hydroxylation process and carbon flux pathways of dihydromyricetin in Ampelopsis grossedentata were successfully identified.
Article
Plant Sciences
Dawei Ma, Hao Tang, Michael Reichelt, Eerik-Mikael Piirtola, Juha-Pekka Salminen, Jonathan Gershenzon, C. Peter Constabel
Summary: Flavonoids are important secondary metabolites in plants, regulated by MYB transcription factors. The study identified that the MYB activator MYB117 can enhance anthocyanin accumulation in hybrid poplar by up-regulating F3'5'H1, resulting in increased B-ring hydroxylation.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biotechnology & Applied Microbiology
Ling Wang, Xiaowei Wu, Chenghua Gao, Lingrui Wei, Qian Li, Aitao Li
Summary: A new cytochrome P450 enzyme, CYP68J5_fg, was identified from Fusarium graminearum, showing steroidal 12 beta- and 15 alpha-hydroxylase activities. This is the first fungal P450 enzyme with 12 beta-hydroxylase activity discovered, offering opportunities for enhancing efficiency and selectivity in steroid drug synthesis.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Praveen Khatri, Ling Chen, Istvan Rajcan, Sangeeta Dhaubhadel, Mojtaba Kordrostami
Summary: Cinnamate 4-hydroxylase (C4H) is an important enzyme in the phenylpropanoid pathway that converts trans-cinnamic acid to p-coumaric acid. This study identified four C4H genes in the soybean genome, with tissue-specific expression patterns. GmC4H2, GmC4H14, and GmC4H20 showed the highest expression levels in root tissues.
Article
Biochemistry & Molecular Biology
Hana Akari Arifin, Takuyu Hashiguchi, Kiyoko Nagahama, Masatsugu Hashiguchi, Melody Muguerza, Yoichi Sakakibara, Hidenori Tanaka, Ryo Akashi
Summary: The authors evaluated the content of isoflavones, total flavonoids, and total phenolics in 26 soybean accessions, and examined their antioxidant activity using an antioxidant responsive element in human HepG2 cells. The results showed that 22 accessions exhibited significant antioxidant activities, and there was a moderate correlation between total isoflavone level and antioxidant activity.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Jingyan Zhang, Ying Sun, Yeji Wang, Xin Chen, Lu Xue, Jingjing Zhang, Xiangcheng Zhu, Yanwen Duan, Xiaohui Yan
Summary: Rubiginones are part of the angucycline family and have been shown to enhance the cytotoxicity of vincristine against drug-resistant cancer cell lines. Through genome mining of Streptomyces sp. CB02414, new rubiginones were discovered and a biosynthetic pathway was proposed based on the production profiles of wild-type and mutant strains. This research paves the way for further studies on cytochrome P450-catalyzed oxidation of angucyclines and the development of novel compounds through combinatorial biosynthesis strategies.
MICROBIAL CELL FACTORIES
(2021)
Review
Agriculture, Dairy & Animal Science
M. J. Kuhn, V Mavangira, L. M. Sordillo
Summary: Dairy cattle are at a high risk of disease around calving due to compromised immunity and oxidative stress, influenced by the metabolism of fatty acids and fat-soluble vitamins. The importance of the cytochrome P450 (CYP450) enzyme family in cattle health is underappreciated, with potential interactions between fatty acids and fat-soluble vitamins needing further exploration. Understanding the role of CYP450 in immune function and oxidant status could help in reducing postpartum disease occurrence.
JOURNAL OF DAIRY SCIENCE
(2021)
Review
Pharmacology & Pharmacy
Kai-Di Ni, Jun-Yan Liu
Summary: CYP omega-hydroxylases are a subfamily of enzymes that mediate the metabolism of fatty acids and their metabolites. The metabolites have pleiotropic effects in inflammation and related diseases. Further studies are needed to understand the function and mechanisms of other CYP omega-hydroxylases-mediated eicosanoids in order to develop therapeutic strategies for various diseases.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuying Hou, Yufei Wang, Xiaoyu Liu, Naveed Ahmad, Nan Wang, Libo Jin, Na Yao, Xiuming Liu
Summary: In this study, a CtC4H1 gene was identified from safflower, regulating flavonoid biosynthesis and antioxidant defense system under drought stress. Overexpressing CtC4H1 in Arabidopsis resulted in wider leaves, long and early stem development, increased levels of metabolites and anthocyanin contents, as well as enhanced antioxidant activity and osmotic balance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Maria Norlin, Kjell Wikvall
Summary: Vitamin D signaling plays a crucial role in maintaining calcium balance for bone health and has various functions in different tissues. Disturbances in vitamin D signaling are associated with numerous diseases. This review focuses on the identification of bioactivating enzymes and their genes involved in the production of active vitamin D metabolites. It evaluates species- and tissue-specific expression, enzyme kinetics, substrate specificity, and the consequences of gene mutations. The roles of different vitamin D receptors and alternative bioactivation pathways are also discussed. Although significant progress has been made in understanding vitamin D bioactivation enzymes, further research is needed to fully comprehend the pleiotropic effects and the mechanisms of enzymatic activation in vitamin D-induced responses.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Plant Sciences
Jiankang Xin, Tianmin Che, Xiaolong Huang, Huiqing Yan, Shan Jiang
Summary: In this study, the functions of PpCYP98 in Physcomitrium patens were investigated using transcriptome and metabolome analyses. A total of 5266 differentially expressed genes (DEGs) and 68 differentially abundant secondary metabolites were identified between wild-type and ∆PpCYP98 gametophores. The results showed that PpCYP98 plays vital roles in the pre-lignin synthesis pathway and the synthesis of flavonoids in bryophytes. PpCYP98 also showed systemic effects on metabolisms, including carbohydrate, fatty acid, and hormonal signaling transductions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Taisei Yamamoto, Yoshie Hasegawa, Hiroaki Iwaki
Summary: Cytochrome P450 monooxygenases are important in metabolism. In this study, a novel self-sufficient cytochrome P450, P450(CHC), was identified and characterized in Paraburkholderia terrae KU-64, which degrades cyclohexanecarboxylate.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2022)
Article
Agriculture, Multidisciplinary
Juan Liu, Zhiqiang Xiao, Siqi Zhang, Zhen Wang, Yun Chen, Yang Shan
Summary: Enzyme promiscuity is advantageous for plants in adapting to the environment, but it can negatively impact the expression of plant enzymes in microorganisms. We demonstrated that refining the promiscuity of two enzymes, F3'H and F4'OMT, improved the production of (2S)-hesperetin in Escherichia coli. By employing molecular docking and directed evolution, we obtained a highly substrate-specific ThF3'H and a promiscuity-restricted MpOMT mutant, resulting in a 14-fold increase in (2S)-hesperetin production and reduced side products. This study highlights the importance of optimizing plant enzyme promiscuity for improving natural product production in microbial cell factories.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Food Science & Technology
Danitza Carrasco, Camila Carrasco, Vanessa Souza-Mello, Cristian Sandoval
Summary: This systematic review suggests that antioxidant treatments may be beneficial and effective in reducing CYP2E1 activity and increasing GSH levels after alcohol exposure, thereby improving oxidative stress. The results indicate that antioxidant treatments could be beneficial and effective during ethanol exposure in human or in vitro models.
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2021)
Article
Plant Sciences
Jieyu Qian, Lingli Jiang, Hongsheng Qing, Jiahong Chen, Ziyun Wan, Menghan Xu, Jianxin Fu, Chao Zhang
Summary: The petal color in Zinnia elegans is determined by the accumulation of anthocyanins, especially cyanidins. The transcription factor ZeMYB9 from subgroup 6 was identified as a positive regulator of anthocyanin accumulation, and it can activate the expression of ZeF3'H, enhancing cyanidin synthesis and regulating petal color in Z. elegans. ZeMYB9 was also found to interact with the bHLH transcription factor ZeGL3, further promoting the promoter activity of ZeF3'H and inducing anthocyanin accumulation in tobacco leaves.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Materials Science, Characterization & Testing
D. F. Jingle Jabha, K. Selvi, R. Joselin
JOURNAL OF TESTING AND EVALUATION
(2018)
Article
Chemistry, Analytical
Emine Kilickaya Selvi, Halbay Turumtay, Adem Demir, Emine Akyuz Turumtay
ANALYTICAL LETTERS
(2018)
Article
Chemistry, Medicinal
Yoshinori Murai, Ryoji Takahashi, Junichi Kitajima, Tsukasa Iwashina
NATURAL PRODUCT COMMUNICATIONS
(2019)
Article
Biotechnology & Applied Microbiology
Tomoko Kuriyama, Setsuko Shimada, Minami Matsui
PLANT BIOTECHNOLOGY
(2019)
Article
Plant Sciences
Fan Yan, Stephen M. Githiri, Yajing Liu, Yu Sang, Qingyu Wang, Ryoji Takahashi
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Plant Sciences
Tomoko Yamaguchi, Yukio Kurihara, Yuko Makita, Emiko Okubo-Kurihara, Ami Kageyama, Emi Osada, Setsuko Shimada, Hiroko Tsuchida, Hiroaki Shimada, Minami Matsui
Article
Biochemistry & Molecular Biology
Preethi Vetrivel, Seong Min Kim, Sang Eun Ha, Hun Hwan Kim, Pritam Bhagwan Bhosale, Kalaiselvi Senthil, Gon Sup Kim
Article
Plant Sciences
Bharathi Pukalenthy, Dhasarathan Manickam, Karthikeyan Adhimoolam, Sandesh Goragundi Mahesh, Nagalakshmi Ramanathan, Sarankumar Chandran, Vellaikumar Sampathrajan, Ravikesavan Rajasekaran, Kavithapushpam Arunachalam, Kalaiselvi Senthil, Vignesh Muthusamy, Firoz Hossain, Senthil Natesan
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2020)
Article
Chemistry, Multidisciplinary
Jin A. Kim, Preethi Vetrivel, Seong Min Kim, Sang Eun Ha, Hun Hwan Kim, Pritam Bhagwan Bhosale, Jeong Doo Heo, Won Sup Lee, Kalaiselvi Senthil, Gon Sup Kim
Summary: The study used proteomic techniques to analyze muscle tissues of young and old rats, revealing differentially expressed proteins between the two age groups. This may lead to the development of protein targets for the treatment of sarcopenia, providing insights into potential therapies for muscle loss.
Review
Pharmacology & Pharmacy
Adhimoolam Karthikeyan, Kim Na Young, Mohammad Moniruzzaman, Anteneh Marelign Beyene, Kyoungtag Do, Senthil Kalaiselvi, Taesun Min
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the small intestine and colon, including ulcerative colitis (UC) and Crohn's disease (CD), which are major factors for the development of colon cancer. Current treatments involve synthetic drugs and monoclonal antibodies with side effects and high relapse rates. Recent studies suggest that curcumin derived from turmeric may be a potential therapeutic agent for IBD, effectively reducing clinical relapse in patients.
Article
Plant Sciences
Masaaki Sakuta, Asuka Tanaka, Kaori Iwase, Mizuki Miyasaka, Sachiko Ichiki, Miho Hatai, Yoriko T. Inoue, Ayumi Yamagami, Takeshi Nakano, Kazuko Yoshida, Setsuko Shimada
Summary: This study revealed that the lack of anthocyanins in betalain-producing Caryophyllales plants is due to the loss of function in MYB transcription factors, suppressing the expression of genes involved in the late stage of anthocyanin synthesis.
JOURNAL OF PLANT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Aishwarya Vetrivel, Janani Ramasamy, Santhi Natchimuthu, Kalaiselvi Senthil, Monica Ramasamy, Rajeswari Murugesan
Summary: This study utilized machine learning, pharmacophore-based virtual screening, molecular docking, and simulation studies to search for new leads as LasR inhibitors by obstructing the quorum sensing network of Pseudomonas aeruginosa. QSAR models were generated using the support vector machine algorithm, and potential leads were identified through pharmacophore screening and molecular docking. The stability of these leads was further examined through molecular dynamics simulation, and their ADME properties were determined. The compounds identified in this study have the potential to be developed as novel inhibitors for LasR.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Plant Sciences
Setsuko Shimada, Yuki Yanagawa, Takachika Munesada, Yoko Horii, Tomoko Kuriyama, Mika Kawashima, Youichi Kondou, Takeshi Yoshizumi, Nobutaka Mitsuda, Masaru Ohme-Takagi, Yuko Makita, Minami Matsui
Summary: Arabidopsis has approximately 2000 transcription factors in its genome, but their overlapping activities have made it difficult to analyze their functions. In this study, researchers generated inducible TF-GR lines in Arabidopsis to uncover clues to their function. By examining one of these TF-GR lines, they identified 13 novel TF candidates and investigated the regulating genes of two TFs showing higher anthocyanin accumulation under light conditions.
Review
Biochemistry & Molecular Biology
Muthuswamy Sathyabhama, Loganathan Chandramani Priya Dharshini, Adhimoolam Karthikeyan, Senthil Kalaiselvi, Taesun Min
Summary: Oxidative stress and mitochondrial dysfunction are closely linked to the development of various human diseases. Curcumin, a bioactive compound found in turmeric, has been shown to reduce oxidative stress and mitochondrial dysfunction, leading to potential therapeutic effects in these diseases.
Article
Thermodynamics
C. K. Selvi, A. N. S. Srinivas
THERMAL SCIENCE AND ENGINEERING PROGRESS
(2018)