Article
Biochemistry & Molecular Biology
Zoran Minic, Nico Huettmann, Suttinee Poolsup, Yingxi Li, Vanessa Susevski, Emil Zaripov, Maxim V. Berezovski
Summary: In this study, the phosphoproteomic analysis of breast cancer-derived small extracellular vesicles (sEVs) provided insights into the molecular and cellular regulatory mechanisms important for breast cancer tumor progression and metastasis. Functional metabolic enzymes were found in sEVs, which could potentially be used for early breast cancer diagnosis and therapy.
Article
Biochemistry & Molecular Biology
Zhongyuan Wang, Vivek Yadav, Xiaoyao Chen, Siyu Zhang, Xinhao Yuan, Hao Li, Jianxiang Ma, Yong Zhang, Jianqiang Yang, Xian Zhang, Chunhua Wei
Summary: This study identified and characterized the molecular network involved in sex determination and female development in melon using comparative multi-omics data analysis. Four biological process modules related to female development were summarized, and important candidate genes with crucial roles in female development were identified. This study provides new insights into the sex differentiation mechanisms in melon.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Jing Li, Senlin Jiang, Guobin Yang, Yanwei Xu, Lujun Li, Fengjuan Yang
Summary: This study determined the molecular mechanism of color formation in eggplants and identified novel genes related to the difference in peel color and anthocyanin biosynthesis. Functional analysis confirmed the important roles of five genes in anthocyanin accumulation in eggplant peels. A regulatory model for the mechanism of color formation in eggplant was proposed.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Sudhaker Dharavath, Khushboo Kumari, Sudhir Kumar, Samudrala Gourinath
Summary: This study reveals the mechanism of insensitivity of serine acetyltransferase EhSAT3 to L-cysteine and identifies the crucial role of the C-terminal region in regulating feedback inhibition.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Jian Lyu, Yue Wu, Xin Jin, Zhongqi Tang, Weibiao Liao, Mohammed Mujitaba Dawuda, Linli Hu, Jianming Xie, Jihua Yu, Alejandro Calderon-Urrea
Summary: This study revealed the essential role of ethylene in adventitious root development in cucumber seedlings, impacting photosynthesis, carbon metabolism, and energy production. Key enzymes involved in adventitious root development were identified, such as SAMS and AtpA. Exogenous ethylene application enhanced endogenous ethylene synthesis, promoting the induction and formation of adventitious roots.
Article
Plant Sciences
Guoyong Fu, Baojun Chen, Xinxin Pei, Xiaoyang Wang, Xiao Wang, Mian Faisal Nazir, Jingjing Wang, Xiaomeng Zhang, Aishuang Xing, Zhaoe Pan, Zhongxu Lin, Zhen Peng, Shoupu He, Xiongming Du
Summary: This study investigates the characteristics of SCPLs in different cotton species and reveals that segmental duplication and whole genome duplication are the main reasons for the increase in the number of genes during cotton evolution. It also identifies Gh_SCPL genes that exhibit differential expression in particular tissues or response to environmental stimuli, providing a new measure for the in-depth characterization of selected genes of importance.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Chao-Jun Pei, Qing-Xia He, Zhipu Luo, Hongwei Yao, Zhi-Xin Wang, Jia-Wei Wu
Summary: This study investigates the biochemical characteristics and three-dimensional structure of MKK5 in Arabidopsis. The results show that recombinant AtMKK5 can phosphorylate and activate its physiological substrate AtMPK6. The kinase domain of AtMKK5 adopts an active conformation, and there are sequence divergences indicating potential distinct modes of upstream kinase/substrate binding.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2022)
Article
Biochemical Research Methods
Zi Wang, Heng Zhou, Xiali Yue, Jiang Zhu, Yunhuang Yang, Maili Liu
Summary: SHIP2-SH2 mainly utilizes two regions to bind with Y292-phosphorylated Fc gamma RIIB-ITIM, with the BG-loop acting as an auxiliary interface. SHIP2-SH2 applies diverse patterns for binding to different ligand proteins, and certain residues are identified to mediate the binding of specific ligands.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Aritra Roy Choudhury, Swapan Kumar Roy, Pankaj Trivedi, Jeongyun Choi, Kun Cho, Sung Ho Yun, Denver Walitang, Jung-Ho Park, Kiyoon Kim, Tongmin Sa
Summary: This study evaluates the proteome changes in rice plants inoculated with ACC deaminase producing bacteria under salt-stress conditions. The findings suggest that inoculation with bacteria can enhance photosynthetic activity, antioxidant activities, and ethylene regulation related proteins, thus attenuating salt-induced apoptosis and sustaining plant growth and development.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Qiao Hu, Lun Yao, Xia Liao, Liang-Sheng Zhang, Hao-Tian Li, Ting-Ting Li, Qing-Gen Jiang, Mei-Fang Tan, Lu Li, Roger R. Draheim, Qi Huang, Rui Zhou
Summary: A comparative study on STK/STP in Streptococcus suis revealed that STK plays a more critical role in cell division, energy metabolism, and protein translation, while STP primarily impacts bacterial growth. Deletion of stk or stp had significant effects on protein expression, with more virulence factors downregulated in increment stk strains. Overall, both STP and STK are essential regulatory proteins for S. suis, with STK playing a more prominent role in growth, cell division, and pathogenesis.
Article
Biochemistry & Molecular Biology
Katarzyna Mrowiec, Marek Chadalski, Anna Paszek, Natalia Vydra, Patryk Janus, Monika Pietrowska, Agnieszka Toma-Jonik, Michalina Gramatyka, Wieslawa Widlak
Summary: The mouse BFARv3 protein interacts with various proteins involved in RNA processing, calcium signaling, and nuclear localization, suggesting its role in RNA metabolism and potential impact on oocyte development. The protein is specifically highly expressed in metaphase II oocytes, indicating its importance in critical developmental stages. This mouse-specific protein has no human ortholog, which may contribute to species differences in oocyte maturation and early embryonic development.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Kunxi Zhang, Xiaofei Chen, Penghao Yuan, Chunhui Song, Shangwei Song, Jian Jiao, Miaomiao Wang, Pengbo Hao, Xianbo Zheng, Tuanhui Bai
Summary: Apple is one of the most cultivated fruit crops in China, but it frequently suffers from waterlogging stress, leading to yellowing leaves and decreased fruit quality and yield. This study investigated the response of two apple rootstocks (M. hupehensis and M. toringoides) to waterlogging stress and found that M. toringoides showed more severe leaf chlorosis and had higher ethylene production. Transcriptomic analysis revealed differentially expressed genes related to flavonoid biosynthesis and hormone signaling between the two rootstocks under waterlogging stress. These findings provide important insights for understanding the mechanisms of waterlogging tolerance in apple and for future molecular breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Ziqian Cui, Shaodong Liu, Changwei Ge, Qian Shen, Siping Zhang, Huijuan Ma, Ruihua Liu, Xinhua Zhao, Ruida Liu, Pengzhen Li, Hongchen Wang, Qidi Wu, Chaoyou Pang, Jing Chen
Summary: Two significantly associated regions were identified for root diameter traits on chromosome A05 and D04, respectively. Two candidate genes related to root development were also identified. Silencing these genes resulted in reduced root length and surface area. This study provides important insights for the cultivation and improvement of cotton varieties.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Multidisciplinary Sciences
Hengwei Zou, Hanxiao Jiang, Liangbo Li, Rongshao Huang
Summary: The study found that the Aspergillus flavus elicitor 1-G4 can promote the growth and development of Bletilla striata, increasing the contents of SA, ICAld, and ME-IAA while decreasing IP and ACC contents. By analyzing transcriptomics and metabolomics, key genes involved in B. striata growth were identified, laying a foundation for the effective utilization of endophytic fungi and the optimization of B. striata germplasm resources.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Fang Hu, Yunxiang Zhang, Jinping Guo
Summary: This study identifies the importance of LOX gene in plant growth, development, and stress response. Different LOX genes have diverse reactions to abiotic stress and hormone treatment, highlighting their varied roles.
Article
Chemistry, Multidisciplinary
Guangshan Xie, Lin Zhu, Yuanyuan Song, Wei Huang, Di Hu, Zongwei Cai
Summary: An integrated quantitative proteomics strategy was established in this study to investigate the acute response to celastrol treatment in a rat macrophage cell line challenged with lipopolysaccharide. The study revealed that celastrol has acute anti-inflammatory effects and may act through a novel dual mechanism. This efficient workflow provides insights for monitoring drug responses.
CHINESE CHEMICAL LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Man Lung Yeung, Jade Lee Lee Teng, Lilong Jia, Chaoyu Zhang, Chengxi Huang, Jian-Piao Cai, Runhong Zhou, Kwok-Hung Chan, Hanjun Zhao, Lin Zhu, Kam-Leung Siu, Sin-Yee Fung, Susan Yung, Tak Mao Chan, Kelvin Kai-Wang To, Jasper Fuk-Woo Chan, Zongwei Cai, Susanna Kar Pui Lau, Zhiwei Chen, Dong-Yan Jin, Patrick Chiu Yat Woo, Kwok-Yung Yuen
Summary: Research has identified the crucial role of human host factors and sACE2 protein in virus dependency factors, cell entry, and other aspects of SARS-CoV-2 infection, shedding light on the pathogenesis of COVID-19 and potential treatment strategies.
Article
Chemistry, Medicinal
Wei Huang, Lin Zhu, Guodong Cao, Peisi Xie, Yuanyuan Song, Jialing Huang, Xiangfeng Chen, Zongwei Cai
Summary: The study found a decrease in body weight gain (although not significant) and identified 52 proteins and 57 metabolites with differential expression in mice exposed to TCS. These differentially expressed proteins and metabolites play important roles in neuronal signaling and function. Bioinformatics analysis revealed that they are involved in glucose metabolism, purine metabolism, neurotransmitter release, and neural plasticity, indicating disruption in energy metabolism, mitochondria function, neurotransmitter system, and neuronal function.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Engineering, Environmental
Hongna Zhang, Katherine Z. Sanidad, Lin Zhu, Julie Parsonnet, Thomas D. Haggerty, Guodong Zhang, Zongwei Cai
Summary: The metabolic activation of TCS in mammals is often through aromatic hydroxylation, with CYP2B6 potentially playing a key role. Multiple oxidative metabolites of TCS were identified in mouse liver, bile, feces, plasma, and urine, showing isomer-specific retention in mice. Isomeric hydroxylated metabolites were also detected in the urine and stool of human subjects using TCS-containing household and personal care products for the first time.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Hongzhi Zhao, Yuanyuan Zheng, Lin Zhu, Li Xiang, Shunqing Xu, Zongwei Cai
Summary: A large longitudinal non-targeted metabolomics study was conducted to investigate the metabolite alterations during the pathogenesis and development process of gestational diabetes mellitus (GDM). The study found significant changes in the levels of various metabolites during different trimesters of pregnancy, as well as differences between the GDM group and the control group. These differential metabolites were involved in multiple metabolic pathways, providing insights into the pathogenesis of GDM.
CHINESE CHEMICAL LETTERS
(2022)
Article
Gastroenterology & Hepatology
Shiyan Wang, Shanshan Gao, Yong Zeng, Lin Zhu, Yulin Mo, Chi Chun Wong, Yi Bao, Peiran Su, Jianning Zhai, Lina Wang, Fraser Soares, Xin Xu, Huarong Chen, Kebria Hezaveh, Xinpei Ci, Aobo He, Tracy McGaha, Catherine O'Brien, Robert Rottapel, Wei Kang, Jianfeng Wu, Gang Zheng, Zongwei Cai, Jun Yu, Housheng Hansen He
Summary: The study identified a novel oncogenic epitranscriptome axis of YTHDF1-m(6)A-ARHGEF2 that regulates CRC tumorigenesis and metastasis.
Article
Chemistry, Multidisciplinary
Siwen Liu, Lin Zhu, Guangshan Xie, Bobo Wing-Yee Mok, Zhu Yang, Shaofeng Deng, Siu-Ying Lau, Pin Chen, Pui Wang, Honglin Chen, Zongwei Cai
Summary: Currently, there is no effective antiviral medication for COVID-19. This study identified protein kinase C (PKC), interleukin-1 receptor-associated kinase 4 (IRAK4), MAP/microtubule affinity-regulating kinase 3 (MARK3), and TANK-binding kinase 1 (TBK1) as potential therapeutic targets for inhibiting SARS-CoV-2 replication based on the alterations in phosphorylation events. Inhibition of PKC, IRAK4, and p38 showed significant antiviral effects and reduced proinflammatory cytokine production. Furthermore, PKC was found to contribute to viral entry, suggesting its importance as a potential target for COVID-19 treatment.
Article
Environmental Sciences
Yuting Shen, Guangshan Xie, Siyi Lin, Lin Zhu, Hongna Zhang, Zhu Yang, Zongwei Cai
Summary: Our study demonstrated the detrimental effects of benzo[a]pyrene on the migration of esophageal cells and proposed a potential mechanism underlying its carcinogenicity, contributing to our understanding of environmental pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Zhitong Zhu, Xiaoming Ma, Lin Zhu, Qiong Luo, Nan Lin, Zhonghui Chen, Xingchen Zhao, Zhenyu Lin, Zongwei Cai
Summary: COVID-19 continues to pose a major threat to human society. A reliable and rapid assay for serum neutralizing antibodies is necessary for pandemic management. Using gold nanoparticles as an immunosensor, a semi-quantitative, instrument-free assay for measuring COVID-19 antibody levels was developed. The method showed good correlation with conventional ELISA assays and could potentially be used for on-site measurement of COVID-19 antibodies.
Article
Engineering, Environmental
Xin Diao, Chengyi Xie, Guangshan Xie, Yuanyuan Song, Yanshan Liang, Ruijin Li, Chuan Dong, Lin Zhu, Jianing Wang, Zongwei Cai
Summary: This study utilized advanced techniques to investigate the spatial distribution of lipids in rat brains after exposure to high levels of PM2.5. The findings suggest a possible association between PM2.5 exposure and neurological damage, potentially mediated by reductions in sulfatides and subsequent neuroinflammation in the corpus callosum region.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Article
Environmental Sciences
Liting Hua, Wu Liu, Yarui Liu, Ming Yang, Beibei Wang, Hongkai Zhu, Lin Zhu, Yiming Yao, Yuqin Zhang, Hongzhi Zhao
Summary: This study investigated the exposure to environmental phenols in a rural area in Northwest China, revealing the presence of various phenols in urine samples with higher levels in females. Age might influence the concentrations of phenols in urine.
ENVIRONMENTAL POLLUTION
(2022)
Article
Chemistry, Physical
Ruipeng Luo, Yuyang Li, Ning Wang, Ruyi Zhong, Lixin Xing, Lin Zhu, Yucheng Wang, Lei Du, Siyu Ye
Summary: Replacing the oxygen evolution reaction with alternative low-overpotential anodic reactions is promising for reducing the cell voltage of water splitting. In this study, a NiFeP@NiFe(OH)x catalyst was designed and synthesized for the 5-hydroxymethylfurfural oxidation reaction (HMFOR), demonstrating better activity and stability compared to the reference catalyst.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Engineering, Environmental
Lin Zhu, Lei Du, Guodong Cao, Zongwei Cai
Summary: Existing and emerging persistent organic pollutants (POPs) pose significant threats to human health and economies. The development of effective mitigation strategies to remove or degrade these chemicals in the environment is crucial due to their persistence. Although various methods for electrocatalytic degradation of POPs have been developed in lab conditions, challenges such as harmful byproducts and limited working time hinder their practical application. This perspective work highlights high-entropy alloys as ideal catalyst materials for electrocatalytic degradation and discusses the use of AI to facilitate the design and testing of electrocatalysts. The challenges, negative effects, and solutions of applying AI in this process are also addressed.
ENVIRONMENTAL SCIENCE-ADVANCES
(2023)
Article
Biochemistry & Molecular Biology
Xiaojuan Liu, Cun Li, Zhixin Wan, Man Chun Chiu, Jingjing Huang, Yifei Yu, Lin Zhu, Jian-Piao Cai, Lei Rong, You-qiang Song, Hin Chu, Zongwei Cai, Shibo Jiang, Kwok-yung Yuen, Jie Zhou
Summary: By comparing the innate immune response of bat and human intestinal organoids, researchers have revealed the mechanism of bat tolerance to coronaviruses. Bat organoids show higher expression levels of antiviral genes and a faster and stronger innate immune response, enabling them to curtail virus propagation in the early phase of infection. The susceptibility of bat organoids to certain viruses matches their susceptibility in bats.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Chengyi Xie, Yanyan Chen, Xiaoxiao Wang, Yuanyuan Song, Yuting Shen, Xin Diao, Lin Zhu, Jianing Wang, Zongwei Cai
Summary: In this study, the combination of FDAA derivatization and TIMS-MS enables the simultaneous separation of 19 pairs of chiral amino acids. The developed methods show promising application prospects in biomarker discovery and biological research.
