Article
Food Science & Technology
Rassoul Mozafarpour, Arash Koocheki, Taco Nicolai
Summary: This study investigated the effect of high intensity ultrasound on the structure and functional properties of grass pea protein isolate. It was found that higher sonication amplitudes and longer times improved the protein solubility and surface hydrophobicity. However, prolonged sonication may reduce the free SH content and thermal stability of the protein.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Guilherme C. Lechuga, Paloma Napoleao-Pego, Carlos M. Morel, David W. Provance, Salvatore G. De-Simone
Summary: This study analyzed the interaction sequences of Hemopexin (Hx) with hemin and hemoglobin, and identified specific amino acid sequences involved in the binding process. The study demonstrated the relevance of histidine residues in heme binding and revealed the protein-protein interaction between Hx and the beta-chain of hemoglobin. These findings have important implications for the therapeutic and diagnostic applications of Hx.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Xiaohu Zhou, Chaohua Zhang, Wenhong Cao, Chunxia Zhou, Huina Zheng, Liangzhong Zhao
Summary: The study reports the more effective method of co-precipitation for producing protein mixtures from pea and grass carp. The results showed that the protein co-precipitates had higher subunit ratios, solubility, and foaming activity compared to isolated protein blends, indicating better functional properties. Co-precipitation was found to be more effective than blending for preparing mixed protein using pea and grass carp.
Article
Biochemistry & Molecular Biology
Dafeng Liu, Cai Yuan, Chenyun Guo, Mingdong Huang, Donghai Lin
Summary: This study reported the crystal structure and functional mechanism of the CpsY protein in Mycobacterium tuberculosis. The researchers found that CpsY has conserved regions and spacer segments surrounding its catalytic cavity, and the residue R419 is strictly conserved in regulating its phosphotransferase activity. In addition, deletion of certain spacer segments drastically increased the enzyme activity of CpsY.
Article
Food Science & Technology
Yanyan Lao, Qianyu Ye, Yong Wang, Jitraporn Vongsvivut, Cordelia Selomulya
Summary: Roasting at 150 degrees C for shorter durations (10 and 20 min) positively affected the solubility and emulsion ability of pea protein concentrates and isolates, while longer duration (30 min) decreased the extraction efficiency. Pre-roasting also altered the structure and functional properties of pea proteins, increasing the emulsion ability index but decreasing the emulsion stability index.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Hong-Guang Zhu, Han-Qi Tang, Yong-Qiang Cheng, Zai-Gui Li, Li-Tao Tong
Summary: The study compared pea proteins obtained by dry and wet separation methods, revealing that dry-separated proteins maintained their native structure while wet-separated proteins had higher purity but significant denaturation. Each type of protein had different functional properties, with dry-separated proteins leading to a soft texture in meat analogues and wet-separated proteins displaying better emulsification and foaming properties.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Libo Liu, Yu Miao, Chengwen Hu, Lu Gao, Weijia He, Hong Chu, Tong Zhang, Chun Li, Wenkui Guo
Summary: The study found that ultrasound treatment can improve the structural and functional properties of pea protein, but secondary treatment is not more effective than primary treatment for an extended period of time.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Food Science & Technology
Xixiang Shuai, Lizhi Gao, Qin Geng, Ti Li, Xuemei He, Jun Chen, Chengmei Liu, Taotao Dai
Summary: This study investigated the effects of different proteolytic enzymes and degrees of hydrolysis on the structural and functional properties of pea protein. The results showed that enzymatic treatment significantly increased the solubility of pea protein and improved its foaming and emulsification properties. The degree of hydrolysis also affected the protein's structure and functionality, with trypsin having the greatest impact.
Article
Engineering, Chemical
Hamed Mahdavian Mehr, Nabilah Binti Abdul Hadi
Summary: In this study, the Pickering stabilizing capacity of Grass pea protein isolate (GPPI) was improved through cold plasma treatment. The plasma-treated particles showed improved interfacial properties, resulting in lower contact angle with oil and smaller oil droplets formation. The stabilized emulsions by the plasma-treated particles exhibited better flocculation and reduced oil droplet coalescence. The best stabilizing capacity was achieved with 18.6 kVpp treatment for 300 s, providing a new method for enhancing protein Pickering stabilization.
JOURNAL OF FOOD ENGINEERING
(2023)
Article
Food Science & Technology
Xiaohu Zhou, Chaohua Zhang, Liangzhong Zhao, Wenhong Cao, Chunxia Zhou, Xin Xie, YuLian Chen
Summary: This study investigated the effect of extraction pH on the functional properties of co-precipitated plant-animal dual-protein. The results showed that alkaline extraction resulted in better functional properties, with Co10 exhibiting superior emulsification and gel properties.
Article
Multidisciplinary Sciences
Zhisen Yang, Jing Xia, Jingjing Hong, Chenxi Zhang, Hong Wei, Wei Ying, Chunqiao Sun, Lianghanxiao Sun, Yanbo Mao, Yongxiang Gao, Shutang Tan, Jiri Friml, Dianfan Li, Xin Liu, Linfeng Sun
Summary: This study reports different conformational structures of Arabidopsis thaliana PIN1, revealing the substrate recognition and transport mechanisms of PIN proteins. These findings are of great significance for understanding the directional auxin transport process in plant development.
Article
Food Science & Technology
Hong Sun, Jialu Sun, Nianxu Dou, Jinzhe Li, Muhammad Altaf Hussain, Jiage Ma, Juncai Hou
Summary: This study comprehensively analyzed and compared eight commercially available pea proteins (PPs). Results showed that the contents of total, soluble, and non-denatured protein varied among the different PP samples. PP-V exhibited higher beta-sheet content and better gelation properties, while PP-IV had the highest denaturing temperature. PP-III and PP-IV had higher solubility and emulsion stability, as well as significant foaming capacity and foaming stability. Principal component analysis ranked PP-IV as the highest among the eight PPs. This study provides important information for evaluating the quality characteristics of PPs and guiding the production of multifunctional PP products.
Article
Chemistry, Applied
Liuyi Chang, Yang Lan, Nonoy Bandillo, Jae-Bom Ohm, Bingcan Chen, Jiajia Rao
Summary: The study investigated the structural and functional attributes, as well as the aroma profile of major fractions of pulse storage proteins isolated from green pea and chickpea. Different protein compositions had significant impacts on the structural and functional properties of proteins. Chickpea protein fractions contained higher typical beany aromatic compounds than green pea protein fractions.
FOOD HYDROCOLLOIDS
(2022)
Article
Agriculture, Multidisciplinary
Wenming Wang, Yuan Wang, Hongfang Xi, Zidan Song, Wenlong Zhang, Leilei Xie, Danyang Ma, Nan Qin, Hongfei Wang
Summary: The extension peptide (EP) is a unique feature of mature plant ferritin and may have protease activity related to iron uptake and release. Our study on the crystal structure of ferritin-1 from Setaria italica revealed that the EP in this monocotyledon plant had a different folding pattern than other plant ferritins, suggesting a novel fold pattern for EPs in monocotyledons. Additionally, our study found additional iron atoms binding in the fourfold channels, highlighting the important role of these channels in iron diffusion.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Yan Yuan Tseng, Matthew A. Sanders, Huamei Zhang, Li Zhou, Chia-Yi Chou, James G. Granneman
Summary: By constructing theoretical structural models and validating them experimentally, we have revealed the key structural and functional residues of ABHD5 in lipid metabolism, providing new insights into the diverse functions of ABHD5 and the pathological mutations associated with CDS.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Sk. Abdul Amin, Kalyan Ghosh, Samayaditya Singh, Insaf Ahmed Qureshi, Tarun Jha, Shovanlal Gayen
Summary: The COVID-19 pandemic poses a global threat to public health, with the novel coronavirus possibly never completely disappearing. Studying enzymes vital for viral replication can accelerate the design of broad-spectrum PLpro inhibitors against infectious SARS-CoV and SARS-CoV-2 in the future.
MOLECULAR DIVERSITY
(2022)
Article
Chemistry, Multidisciplinary
Bandigi Narsimulu, Rahila Qureshi, Pranay Jakkula, Sayanna Are, Insaf Ahmed Qureshi
Summary: The study focuses on cloning, purification, and characterization of Ribulose-5-phosphate epimerase (RPE) of Leishmania donovani, revealing unique structural features of the enzyme. Biophysical and structural approaches showed the dimeric state of the enzyme and variations in alpha helical content under different conditions, while molecular docking and dynamics simulations provided insights into substrate and ligand binding. The research suggests that understanding the structure of leishmanial RPE may lead to the development of effective antileishmanial drugs.
Review
Biochemistry & Molecular Biology
Mayank Saini, Sayanna Are, Insaf Ahmed Qureshi
Summary: Human parasites cause deadly diseases, but there is no effective vaccine. Vitamin B6 plays a crucial role in parasites and the enzyme PdxK can be a potential target for drug discovery.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2022)
Article
Virology
Vijay Rani Rajpal, Shashi Sharma, Avinash Kumar, Samantha Vaishnavi, Apekshita Singh, Deepmala Sehgal, Mughdha Tiwari, Shailendra Goel, Soom Nath Raina
Summary: This study investigated the evolution of the SARS-CoV-2 spike protein during the first and second wave of COVID-19 infections in India. Mutation analysis revealed 242 mutations, including 51 novel mutations with higher transmissibility and immune evasion functions.
Review
Agronomy
Dhananjay Raturi, Manju Chaudhary, Vishnu Bhat, Shailendra Goel, Soom Nath Raina, Vijay Rani Rajpal, Apekshita Singh
Summary: The genus Oryza consists of cultivated and wild species, with cultivated rice being a staple food and a model crop for genomic studies. To enhance the characterization and utilization of rice germplasm, core collections and mini core collections have been developed, providing maximum diversity and wider genetic coverage in manageable numbers.
Article
Agronomy
Suman Sharma, Avinash Kumar, Vijay Rani Rajpal, Apekshita Singh, Sadhana Babbar, Soom Nath Raina
Summary: In this study, the genetic diversity of 42 tea clones was analyzed using RAPD and ISSR markers. The results showed that tea has a narrow genetic base and high genetic variation. Population structure analysis revealed diverse genetic ancestry of the tea clones. Some superior clones were found to be genetically distinct and could be utilized in future tea breeding programs.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Review
Virology
Vijay Rani Rajpal, Shashi Sharma, Deepmala Sehgal, Apekshita Singh, Avinash Kumar, Samantha Vaishnavi, Mugdha Tiwari, Hemal Bhalla, Shailendra Goel, Soom Nath Raina
Summary: This review provides a comprehensive overview of COVID-19 onset, SARS-CoV-2 genome architecture, emergence of novel viral lineages, and the development of vaccines. The continuously evolving SARS-CoV-2 has acquired a larger number of mutations, leading to increased transmission, pathogenesis, and immune escape potential. These mutations have also resulted in reduced efficacy of vaccines and antibodies currently in use. Therefore, there is a need for developing next-generation vaccines and therapeutics targeting more conservative genomic regions for adequate protection.
Article
Biochemistry & Molecular Biology
Manisha Sharma, Manju Chaudhary, Soom Nath Raina, Dinabandhu Sahoo, Neel Sarovar Bhavesh, Rakesh Kr Thakur, Vijay Rani Rajpal, Dhananjay Raturi, Apekshita Singh
Summary: This research analyzed the genetic diversity and population structure of black rice cultivars in Manipur, North East India, which has distinct geographical and agroclimatic variations. The results showed that the black rice cultivars in Manipur formed two main groups, with the hill black rice cultivars being more diverse. The population structure analysis revealed seven subpopulations, indicating high genetic variability. Some diverse black rice cultivars, such as Amubi, Chedo Anal, and Chipi Buh, were identified and can be used in future black rice breeding programs to prevent genetic erosion and protect intellectual property rights.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Bandigi Narsimulu, Rahila Qureshi, Pranay Jakkula, Priti Singh, Mohammed Arifuddin, Insaf Ahmed Qureshi
Summary: In this study, the authors purified and characterized seryl tRNA synthetase (LdSerRS) and its mutants in Leishmania donovani. The purified LdSerRS showed high enzymatic activity and a higher content of alpha helices in secondary structure. A mutation in the ATP binding residues resulted in a reduced affinity for cofactor without significant alteration in secondary structure. The researchers identified Comp 5l as a specific inhibitor for leishmanial SerRS, which showed low potency towards purified HsSerRS. Structural analysis revealed the binding of Comp 5l into the cofactor binding site of LdSerRS with high affinity. The study provides a promising approach for developing specific therapeutics targeting leishmanial SerRS.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Plant Sciences
Vijay Rani Rajpal, Apekshita Singh, Renu Kathpalia, Rakesh Kr. Thakur, Mohd. Kamran Khan, Anamika Pandey, Mehmet Hamurcu, Soom Nath Raina
Summary: Crop wild relatives (CWRs), landraces, and exotic germplasm are important sources of genetic variability and useful crop traits. In the pulse crop genus Lens, cultivated varieties have a narrow genetic base, while collecting and characterizing wild Lens germplasm resources have offered new avenues for genetic improvement and development of stress-tolerant lentil varieties. Advances in genetic diversity studies, genome mapping, and high-throughput sequencing technologies have identified stress-responsive adaptive genes and quantitative trait loci (QTLs) in CWRs.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Fouzia Nasim, Insaf Ahmed Qureshi
Summary: The ensemble of aminoacyl tRNA synthetases is a crucial component of the protein translation machinery and is considered an important target for the development of antimicrobial drugs. Studies on tRNA synthetase-ligand complexes have provided a detailed understanding of these enzymes, which play a critical role in decoding the genetic code. In addition to their role in antimicrobial drug development, aminoacyl tRNA synthetases have also been validated as important for the survivability of trypanosomatids, leading to the search for inhibitors that specifically target these enzymes.
Article
Biochemistry & Molecular Biology
Sourav Sardar, Jyotisha, Sk. Abdul Amin, Samima Khatun, Insaf Ahmed Qureshi, Umesh Kumar Patil, Tarun Jha, Shovanlal Gayen
Summary: HDAC1 is an important target for cancer epigenetics and different molecular modeling techniques have been used to study its interactions with natural inhibitors. By analyzing the molecular structures of these inhibitors, certain amino acid residues have been identified as potential binding sites. The stability of the HDAC1-natural inhibitors complexes has also been evaluated.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Bandigi Narsimulu, Pranay Jakkula, Rahila Qureshi, Fouzia Nasim, Insaf Ahmed Qureshi
Summary: This study explores the functional and structural aspects of Leishmania donovani glutamyl-tRNA synthetase (LdGluRS), an essential component in protein synthesis and a potential therapeutic target against parasitic diseases. The study demonstrates the competitive inhibition of LdGluRS by salicylate, which has negligible effect on human macrophages. The role of histidine as a catalytic residue is also highlighted. The findings provide insights into the molecular inhibition and structural features of glutamyl-tRNA synthetase from kinetoplastid parasites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Immunology
Jyotisha, Rahila Qureshi, Insaf Ahmed Qureshi
Summary: This study aims to design and evaluate a multi-epitope vaccine against Leishmaniasis. The designed vaccine was highly antigenic, non-allergenic, and non-toxic, with suitable physicochemical properties. Molecular docking and simulation studies confirmed the high binding affinity and stable interactions between human toll-like receptors and the vaccine. In addition, the vaccine was codon optimized, cloned, and expressed in a bacterial host, showing promising potential as a vaccine candidate against Leishmania parasites.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jyotisha, Samayaditya Singh, Insaf Ahmed Qureshi
Summary: COVID-19, caused by SARS-CoV-2, has resulted in significant global deaths, emphasizing the urgent need for vaccine development. The use of immunoinformatics to design a multi-epitope vaccine may provide specific immune responses against SARS-CoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)