Article
Plant Sciences
Xudong Ye, Zarir Vaghchhipawala, Edward J. Williams, Changlin Fu, Jinyuan Liu, Fengming Lu, Erin L. Hall, Shirley X. Guo, LaRee Frank, Larry A. Gilbertson
Summary: Efficient selectable marker gene autoexcision in transgenic plants of soybean, cotton, canola, and maize is achieved by effective Cre recombinase expression. Different crops require diverse promoters to drive Cre expression, and the efficiency of marker-free progeny generation varies among different crops.
PLANT CELL REPORTS
(2023)
Article
Multidisciplinary Sciences
Savio D. Rodrigues, Mansour Karimi, Lennert Impens, Els Van Lerberge, Griet Coussens, Stijn Aesaert, Debbie Rombaut, Dominique Holtappels, Heba M. M. Ibrahim, Marc Van Montagu, Jeroen Wagemans, Thomas B. Jacobs, Barbara De Coninck, Laurens Pauwels
Summary: Agrobacterium spp. are important plant pathogens that can be genetically modified using CRISPR-mediated base editing to introduce targeted point mutations, improving the efficiency and functionality of the strains for plant transformation and gene editing. The method developed in this study has shown high efficiency and potential for future advancements in engineering Agrobacterium strains.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Yongyu Zhao, Dongxu Yang, Yumei Liu, Fengqing Han, Zhansheng Li
Summary: In this study, the effects of different factors on the genetic transformation efficiency of broccoli were explored. Hypocotyl explants were found to be more suitable for transgene and gene editing. The usage of hygromycin B at a concentration of 5 mg/L was more advantageous for selecting resistant broccoli sprouts, and genotype 19B42 exhibited the highest transformation rate. Additionally, subcellular localization confirmed the expression of glucoraphanin metabolism-related gene Bol031350 and clubroot resistance gene CRa in the cytoplasm and nucleus. These findings contribute to the improvement of genetic transformation and molecular breeding in Brassica oleracea crops.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Kari Johnson, Uyen Cao Chu, Geny Anthony, Emily Wu, Ping Che, Todd J. Jones
Summary: Two wheat transformation methods using morphogenic regulator genes, with or without gene excision, have been developed. These methods achieve independent transformation efficiencies of up to 58% and 75%, respectively, and significantly reduce tissue culture duration. The procedures are simplified, less labor-intensive, higher-throughput, and cost-effective.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Zhelong Lin, Lei Chen, Shanjie Tang, Mengjie Zhao, Tong Li, Jia You, Changqing You, Boshu Li, Qinghua Zhao, Dongmei Zhang, Jianli Wang, Zhongbao Shen, Xianwei Song, Shuaibin Zhang, Xiaofeng Cao
Summary: The lack of genome editing platforms has hindered the study and improvement of forage crops that can be grown on lands not suitable for other crops. This study established an efficient CRISPR/Cas9 genome editing method in a stress-tolerant forage grass called sheepgrass, and knocking out the TB1 gene significantly increased tiller number and biomass.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Min Zhou, Jin Luo, Dong Xiao, Aiqin Wang, Longfei He, Jie Zhan
Summary: This study developed a pollen tube-mediated Agrobacterium tumefaciens transformation method and successfully transformed the AhBI-1 gene into peanut. It was found that overexpression of the AhBI-1 gene enhanced root and shoot growth in peanut.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Review
Plant Sciences
Geoffrey Thomson, Lauren Dickinson, Yannick Jacob
Summary: Agrobacterium-mediated transformation is a commonly used tool in agricultural biotechnology. However, the extent of genomic disruption associated with this method remains underappreciated. New technologies and previous research have shed light on the molecular mechanisms and consequences of DNA integration into plant genomes using Agrobacterium.
Article
Horticulture
Meng Zhang, Sichao Yang, Wenxing Chen, Liqing Xu, Dayong Guo, Zhengrong Luo, Qinglin Zhang
Summary: This study developed an efficient transformation system and in vivo regeneration protocol for persimmon. RNA interference of DkANR gene was successfully achieved, resulting in a decrease in proanthocyanidin accumulation. The reliable and highly efficient transformation system achieved in this study may contribute to gene function identification and genetic improvement in persimmon.
Article
Plant Sciences
Xudong Ye, Ashok Shrawat, Lorena Moeller, Rebecca Rode, Anatoly Rivlin, David Kelm, Brian J. Martinell, Edward J. Williams, Anthony Paisley, David R. Duncan, Charles L. Armstrong
Summary: Transgenic wheat plants with fertility have been successfully regenerated through direct transformation of mechanically isolated mature embryos from field-grown seed. Centrifugation of the embryos in the presence of Agrobacterium is important for efficient T-DNA delivery. The transformed embryos formed multiple buds/shoots on high-cytokinin medium and directly regenerated into transgenic shoots on hormone-free medium. Rooted transgenic plantlets were obtained within 10-12 weeks. Optimization of the protocol reduced the occurrence of chimeric plants to below 5%.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Horticulture
Lisha Gong, Min Lu, Huaming An
Summary: This study demonstrates that Agrobacterium-mediated transformation is a simple, fast, and efficient approach for generating composite R. roxburghii plants.
Article
Plant Sciences
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao
Summary: This study developed a regeneration system for Chinese cabbage and established a simple and efficient genetic transformation method. The method can obtain transformants in a relatively short period of time, paving the way for further gene editing and functional studies.
HORTICULTURAL PLANT JOURNAL
(2021)
Review
Plant Sciences
Madhu Tiwari, Arun Kumar Mishra, Debasis Chakrabarty
Summary: Plants respond to Agrobacterium infection by mounting a three-layered immune response, which determines their susceptibility or resistance to the pathogen. Understanding the molecular dialogues between plants and Agrobacterium may provide important insights for the treatment of crown gall disease.
Article
Biochemistry & Molecular Biology
Shuang-Shuang Wen, Xiao-Lan Ge, Rui Wang, Hai-Feng Yang, Yu-E. Bai, Ying-Hua Guo, Jin Zhang, Meng-Zhu Lu, Shu-Tang Zhao, Liu-Qiang Wang
Summary: In this study, a callus-based transformation method was developed for efficient generation of transgenic plants in hybrid poplar 84K. The optimized protocol showed high efficiency and reproducibility, with a transformation frequency greater than 50%. The method was also found to maintain its high efficiency even after multiple subcultures, making it a valuable reference for developing effective transformation protocols for other poplar species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Alka Jangra, Siddhant Chaturvedi, Garima Sharma, Sonia Sihag, Siddharth Tiwari, Vinod Chhokar
Summary: This study aims to optimize Agrobacterium-mediated transformation in Aloe vera by refining several parameters to improve the infection efficiency. The results showed that an improved transformation efficiency of over 2% was obtained.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Agronomy
Yue Zhang, Chunxiao Qin, Shijia Liu, Yue Xu, Ying Li, Yongxue Zhang, Yingying Song, Meihong Sun, Chunxiang Fu, Zhi Qin, Shaojun Dai
Summary: This study successfully established an Agrobacterium-mediated transformation system for Alkaligrass, laying a foundation for further gene function analysis.
MOLECULAR BREEDING
(2021)
Article
Biotechnology & Applied Microbiology
Qing Liu, Man Wu, Baolong Zhang, Pushkar Shrestha, James Petrie, Allan G. Green, Surinder P. Singh
PLANT BIOTECHNOLOGY JOURNAL
(2017)
Article
Biochemistry & Molecular Biology
Anna El Tahchy, Kyle B. Reynolds, James R. Petrie, Surinder P. Singh, Thomas Vanhercke
Article
Biotechnology & Applied Microbiology
Thomas Vanhercke, Uday K. Divi, Anna El Tahchy, Qing Liu, Madeline Mitchell, Matthew C. Taylor, Peter J. Eastmond, Fiona Bryant, Anna Mechanicos, Cheryl Blundell, Yao Zhi, Srinivas Belide, Pushkar Shrestha, Xue-Rong Zhou, Jean-Philippe Ral, Rosemary G. White, Allan Green, Surinder P. Singh, James R. Petrie
METABOLIC ENGINEERING
(2017)
Article
Biotechnology & Applied Microbiology
Kyle B. Reynolds, Matthew C. Taylor, Darren P. Cullerne, Christopher L. Blanchard, Craig C. Wood, Surinder P. Singh, James R. Petrie
PLANT BIOTECHNOLOGY JOURNAL
(2017)
Article
Biochemical Research Methods
Srinivas Belide, Thomas Vanhercke, James Robertson Petrie, Surinder Pal Singh
Article
Plant Sciences
Yao Zhi, Matthew C. Taylor, Peter M. Campbell, Andrew C. Warden, Pushkar Shrestha, Anna El Tahchy, Vivien Rolland, Thomas Vanhercke, James R. Petrie, Rosemary G. White, Wenli Chen, Surinder P. Singh, Qing Liu
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Plant Sciences
Robert S. Allen, Kimberley Tilbrook, Andrew C. Warden, Peter C. Campbell, Vivien Rolland, Surinder P. Singh, Craig C. Wood
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Biotechnology & Applied Microbiology
Lin Xu, Lian Wang, Xue-Rong Zhou, Wen-Chao Chen, Surinder Singh, Zhe Hu, Feng-Hong Huang, Xia Wan
BIOTECHNOLOGY FOR BIOFUELS
(2018)
Article
Biotechnology & Applied Microbiology
Thomas Vanhercke, Srinivas Belide, Matthew C. Taylor, Anna El Tahchy, Shoko Okada, Vivien Rolland, Qing Liu, Madeline Mitchell, Pushkar Shrestha, Ingrid Venables, Lina Ma, Cheryl Blundell, Anu Mathew, Lisa Ziolkowski, Nathalie Niesner, Dawar Hussain, Bei Dong, Guoquan Liu, Ian D. Godwin, Jiwon Lee, Melanie Rug, Xue-Rong Zhou, Surinder P. Singh, James R. Petrie
PLANT BIOTECHNOLOGY JOURNAL
(2019)
Review
Biochemistry & Molecular Biology
Thomas Vanhercke, John M. Dyer, Robert T. Mulle, Aruna Kilaru, Md. Mahbubur Rahman, James R. Petrie, Allan G. Green, Olga Yurchenko, Surinder P. Singh
PROGRESS IN LIPID RESEARCH
(2019)
Article
Plant Sciences
James R. Petrie, Xue-Rong Zhou, Antonio Leonforte, Jason McAllister, Pushkar Shrestha, Yoko Kennedy, Srinivas Belide, Greg Buzza, Nelson Gororo, Wenxiang Gao, Geraldine Lester, Maged P. Mansour, Roger J. Mulder, Qing Liu, Lijun Tian, Claudio Silva, Noel O. Cogan, Peter D. Nichols, Allan G. Green, Robert de Feyter, Malcolm D. Devine, Surinder P. Singh
FRONTIERS IN PLANT SCIENCE
(2020)
Letter
Biotechnology & Applied Microbiology
Srinivas Belide, Pushkar Shrestha, Yoko Kennedy, Antonio Leonforte, Malcolm D. Devine, James R. Petrie, Surinder P. Singh, Xue-Rong Zhou
PLANT BIOTECHNOLOGY JOURNAL
(2022)