Article
Agronomy
Gaddam Prasanna Kumar, Pooja Pathania, Nitu Goyal, Nishu Gupta, R. Parimalan, J. Radhamani, Sunil Shriram Gomashe, Palchamy Kadirvel, S. Rajkumar
Summary: This study evaluated the genetic diversity of 3115 safflower accessions from the Indian National Gene Bank and developed a manageable set of accessions with similar genetic variations. The results showed that the safflower germplasm was highly diverse and there was no correlation between genetic diversity and geographical collection. A core set of 351 accessions was developed using core hunter software.
Article
Horticulture
Sara Barrias, Leonor Pereira, Sara Rocha, Tiago Alves de Sousa, Javier Ibanez, Paula Martins-Lopes
Summary: Precise identification of ancient grapevine varieties is crucial but often hampered by synonyms and misidentifications. In this study, a combination of SSR-Multiplex PCR, SNP genotyping, and HRM was used to identify 19 varieties in a Portuguese vineyard. The results revealed new somatic variants and demonstrated the usefulness of HRM in variety identification.
SCIENTIA HORTICULTURAE
(2023)
Article
Multidisciplinary Sciences
Kalliopi Kadoglidou, Catherine Cook, Anastasia Boutsika, Eirini Sarrou, Ifigeneia Mellidou, Christina Aidonidou, Ioannis Grigoriadis, Andrea Angeli, Stefan Martens, Vasiliki Georgiadou, Theodoros Moysiadis, Parthenopi Ralli, Ioannis Mylonas, Nikolaos Tourvas, Michail Michailidis, Apostolos Kalivas, Eleni Maloupa, Ioannis Ganopoulos, Aliki Xanthopoulou
Summary: Greek landraces of dill have distinct morphological, genetic, and chemical traits compared to modern commercial cultivars. Landraces have desirable characteristics such as taller height, larger umbels, and denser foliage. Genetic divergence exists between landraces and cultivars, but some gene flow may have occurred. Greek landraces of dill have potential for breeding programs to develop improved cultivars.
Article
Genetics & Heredity
Aida Dervishi, Jernej Jakse, Hairi Ismaili, Branka Javornik, Natasa Stajner
Summary: This study investigated the genetic diversity and structure of Albanian olive germplasm using 26 microsatellite markers. The findings revealed that Albanian olive germplasm is a unique gene pool and provides an interesting genetic basis for breeding programs. A core collection of 57 genotypes representing all allelic richness found in Albanian germplasm was developed for the first time.
Article
Food Science & Technology
Irini Bosmali, Georgios Lagiotis, Evangelia Stavridou, Nadia Haider, Maslin Osathanunkul, Kostas Pasentsis, Panagiotis Madesis
Summary: Coffee, a popular and heavily traded commodity, is often targeted by fraudulent practices. However, through the use of an ITS2-based marker and HRM analysis, coffee authenticity can be effectively determined, even from brewed beverages.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Anthoula Gleridou, Ioannis Tokatlidis, Alexios Polidoros
Summary: Genetic differentiation among 40 lentil genotypes was tested using molecular markers. Results showed low variation among consecutive generations produced via honeycomb breeding methodology. Molecular analysis revealed that the variation was higher within each generation's population than between them. Population structure analysis indicated that ongoing selection could effectively alter the allelic composition in each generation.
APPLIED SCIENCES-BASEL
(2022)
Article
Microbiology
Guillaume Girault, Ludivine Perrot, Virginie Mick, Claire Ponsart
Summary: In this study, a high-resolution melting (HRM) method was developed for the rapid identification and classification of the Brucella genus based on 17 specific single nucleotide polymorphisms (SNPs) and SNP analysis. This genotyping tool provides a new and quick alternative for Brucella identification, significantly saving time for the diagnosis of brucellosis.
Article
Plant Sciences
Alibek Zatybekov, Moldir Yermagambetova, Yuliya Genievskaya, Svetlana Didorenko, Saule Abugalieva
Summary: This study analyzed 100 soybean accessions and found significant genetic diversity differences among different origin groups. Plant height was identified as an important agronomic trait. The study also discovered significant associations between certain SSR markers and agronomic traits.
Article
Agriculture, Dairy & Animal Science
H. D. Blackburn, B. C. Krehbiel
Summary: This study evaluated the genetic diversity of several traditional chicken breeds in the United States and aimed to provide baseline data for the conservation of their genetic resources. The results showed that there is significant genetic variability within these breeds' populations, and the gene bank has successfully captured this diversity for future use.
Article
Plant Sciences
Bin Li, Noor Muhammad, Shufeng Zhang, Yunxin Lan, Yihan Yang, Shoukun Han, Mengjun Liu, Meng Yang
Summary: Jujube is a commercially important tree native to China due to its high nutritional value, widespread distribution, and diverse germplasm resources. The cultivation of jujube holds potential in providing food security and income for people living in arid and semi-arid regions. Simple Sequence Repeat (SSR) markers are essential for evaluating the genetic diversity and germplasm resources of jujube. However, traditional methods of SSR development are time-consuming and inefficient. A novel approach called Multiple-Genome-Based SSR identification (MGB-SSR) was developed to quickly screen for polymorphic SSRs in the jujube genome. This study successfully differentiated jujube germplasm using 12 SSR markers and provided valuable information for jujube researchers and breeders.
Article
Plant Sciences
Marcello Borin, Fabio Palumbo, Alessandro Vannozzi, Francesco Scariolo, Gio Batta Sacilotto, Marco Gazzola, Gianni Barcaccia
Summary: This study developed a set of simple sequence repeat markers for genotyping high breeding value Cannabis sativa lines, successfully extracting information on homozygosity, genetic uniformity, and genetic variation. Population structure analysis identified eight genetic groups, clustering individuals based on sexual behaviors and geographical origins. The study provides important tools for genetic characterization, authentication, conservation of biodiversity, genetic improvement, and traceability of this increasingly important plant species.
Article
Pharmacology & Pharmacy
Leonardo Anabalon, Jaime Solano, Francisco Encina-Montoya, Marco Bustos, Alejandra Figueroa, David Gangitano
Summary: A rapid, effective, and low-cost method based on nuclear DNA and chloroplast DNA was developed to screen and detect contamination in commercial cannabis seeds. This method can serve as a quality control tool for cannabis seeds and other plant materials.
CANNABIS AND CANNABINOID RESEARCH
(2022)
Article
Agronomy
Maria Victoria Gomez-Rodriguez, Carmen Beuzon, Juan J. Gonzalez-Plaza, Ana Maria Fernandez-Ocana
Summary: Simple Sequence Repeat (SSR) or microsatellite markers are valuable tools in genetic studies, with a new set of tetra- and hexa-nucleotide SSR markers being developed for olive tree cultivar identification. The multiplex PCR protocol allows for efficient differentiation between olive cultivars using only a few SSR markers. Additionally, the use of a subset of SSR markers can successfully distinguish any cultivar from a core collection of olive germplasm.
GENETIC RESOURCES AND CROP EVOLUTION
(2021)
Article
Food Science & Technology
Kaan Hurkan, Menekse Bulut
Summary: A DNA-based high resolution melting (HRM) assay was developed to accurately identify the animal species sources in milk and detect cross-adulteration. This method showed high specificity based on nucleotide variations and could detect adulteration down to 0.5% and 1% for certain species. It provides a rapid, sensitive, and cost-effective tool for milk authentication.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Plant Sciences
Feifei Wu, Shangxiong Zhang, Qiu Gao, Fang Liu, Jianli Wang, Xianguo Wang
Summary: In this study, genetic variation of Vicia amoena populations was assessed using newly developed SSR markers, revealing high genetic diversity within populations. The clustering of V. amoena populations based on habitat types, rather than geographical origins, suggests a complex genetic structure that warrants further investigation. The results highlight the potential of SSR markers as valuable tools for genetic research, breeding improvement, and germplasm resource conservation in V. amoena.
Article
Genetics & Heredity
Aphrodite Tsaballa, Ilektra Sperdouli, Evangelia V. Avramidou, Ioannis Ganopoulos, Athanasios Koukounaras, Georgios K. Ntinas
Summary: The study found that at lower root-zone temperatures, the DNA methylation levels and photosynthetic apparatus systems of rocket plants are altered, but they can still grow efficiently. This suggests that rocket can effectively grow in suboptimal yet sustainable temperatures.
Article
Cell Biology
Maria Ganopoulou, Michail Michailidis, Lefteris Angelis, Ioannis Ganopoulos, Athanassios Molassiotis, Aliki Xanthopoulou, Theodoros Moysiadis
Summary: Genome-wide transcriptome analysis provides crucial data on plant biology at a systemic level. To understand the relationships between proteins and genes in plants, a further thorough analysis at the proteogenomic level is necessary. In this study, a quantitative proteogenomic atlas of sweet cherry tissues was generated to assess the structure of gene/protein relations and their biological implications, revealing the complex nature of causal relations among genes/proteins important for traits in perennial fruit trees. Causal modeling has been employed for the first time within the proteogenomics concept in plants, highlighting persistent relations at the gene/protein level.
Article
Plant Sciences
Kalliopi Kadoglidou, Maria Irakli, Anastasia Boutsika, Ifigeneia Mellidou, Nikolas Maninis, Eirini Sarrou, Vasiliki Georgiadou, Nikolaos Tourvas, Nikos Krigas, Theodoros Moysiadis, Katerina Grigoriadou, Eleni Maloupa, Aliki Xanthopoulou, Ioannis Ganopoulos
Summary: The traditionally edible aerial parts of rock samphire could be a valuable functional food or feed ingredient due to their high antioxidant capacity, ascorbic acid content, and rich content in secondary metabolites such as phenolics and flavonoids. This study evaluated eighteen genotypes from different regions of Greece regarding their phytochemical contents and antioxidant capacity, and also characterized the molecular aspects of native Greek C. maritimum genotypes. The results showed great variation among genotypes in terms of antioxidant capacity and phytochemical content, and low genetic diversity between populations.
Article
Agronomy
Aristeides P. Papapanagiotou, Christos A. Damalas, Irene Bosmali, Panagiotis Madesis, Georgios Menexes, Ilias Eleftherohorinos
Summary: Field and pot experiments in Greece revealed resistance in silky windgrass to certain herbicides. The resistance to chlorsulfuron was confirmed in most populations, while other ALS inhibitors provided adequate control. The gene sequencing showed mutations causing resistance. It is crucial to adopt integrated management practices to delay the evolution of resistant populations due to limited chemistries/modes of action for grass weed control in cereals.
Article
Plant Sciences
Irene Bosmali, Georgios Lagiotis, Nadia Haider, Maslin Osathanunkul, Costas Biliaderis, Panagiotis Madesis
Summary: By analyzing DNA barcoding regions, this study identified 71 Vicia specimens of Eurasian descent. The trnL and ITS2 regions were effective in discriminating the analyzed species, while the rpoC1 region could not identify all selected species due to sequence conservation or missing sequences in GenBank. The combination of DNA barcoding regions is essential for accurate species discrimination within the Vicia genus.
Article
Food Science & Technology
Bastien Anthoons, Georgios Lagiotis, Andreas D. Drouzas, Hugo de Boer, Panagiotis Madesis
Summary: This study developed a high-resolution melting DNA-based protocol for discriminating between phenotypically similar toxic and edible plant species in mixtures, even in simulated gastric acid, which has important implications for application in medicine, forensics, and the agricultural industry.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Chemistry, Analytical
Xanthoula Eirini Pantazi, Anastasia L. Lagopodi, Afroditi Alexandra Tamouridou, Nathalie Nephelie Kamou, Ioannis Giannakis, Georgios Lagiotis, Evangelia Stavridou, Panagiotis Madesis, Georgios Tziotzios, Konstantinos Dolaptsis, Dimitrios Moshou
Summary: The aim of this study was to develop supervised self-organizing map (SOM) models for automatic recognition of systemic resistance state in plants after resistance inducer application. The models were validated using fluorescence kinetics data obtained from a handheld fluorometer. The best performing model achieved 97.22% to 100% accuracy and was confirmed using gene expression data.
Article
Forestry
Maslin Osathanunkul, Nipitpong Sawongta, Panagiotis Madesis, Wittaya Pheera
Summary: This study investigated the application of a hybrid method of DNA barcoding and high-resolution melting analysis (Bar-HRM) for native plant species confirmation. The nuclear ribosomal internal transcribed spacer (ITS) region was identified as a primer set that can clearly discriminate native plant species. The study demonstrated the usefulness of Bar-HRM in native plant species confirmation, even without molecular data.
Article
Biology
Evangelia Stavridou, Ioanna Karamichali, Georgios Lagiotis, Elena Patsea, Maslin Osathanunkul, Panagiotis Madesis
Summary: This study investigated the effects of seasonal changes on the soil microbiome associated with runner bean cultivation. The presence of the runner bean differentially shaped the soil microbial community structure, which is important for understanding the role of soil microbiomes in agriculture.
Article
Plant Sciences
Lefkothea Karapetsi, Emmanouil Pratsinakis, Fotis Xirakias, Maslin Osathanunkul, Ioannis Vagelas, Panagiotis Madesis
Summary: The destructive effects of Fusarium wilts on crop production have been mitigated through soil fumigation and the use of fungicides. However, due to restrictions on residue tolerances and environmental impacts, alternative methods using natural compounds like essential oils are being investigated. Oregano essential oil has been found to inhibit the growth of several fungal species, including Fusarium oxysporum. This study aims to utilize metabarcoding to evaluate the impact of oregano essential oil on F. oxysporum and other fungal species present in soil samples.
Article
Plant Sciences
Eleni Avramidou, Efi Sarri, Ioannis Ganopoulos, Panagiotis Madesis, Leonidas Kougiteas, Evgenia-Anna Papadopoulou, Konstantinos A. Aliferis, Eleni M. Abraham, Eleni Tani
Summary: This study compared the genetic diversity and seed metabolite profiles of five genetic materials of faba bean. Genetic diversity was assessed by SCoT markers and genetic distances were estimated by UPGMA dendrogram. The advanced line KK18 displayed the best nutritional profile and showed the highest potential for further exploration in breeding programs.
Article
Plant Sciences
Eleftherios Karapatzak, Olga Dichala, Katerina Papanastasi, Ioannis Manthos, Ioannis Ganopoulos, Antonis Karydas, Anastasia V. Badeka, Ioanna S. Kosma, Dimitris Kyrkas, Paraskevi Yfanti, Nikolaos Nikisianis, Giorgos Patakioutas, Eleni Maloupa, Nikos Krigas
Summary: This study presents a multifaceted approach for the sustainable agronomic exploitation of four neglected and underutilized forest fruit trees and shrubs in Greece. The evaluation of the Greek germplasm and the feasibility and readiness timescale evaluation showed promising results, indicating the high exploitation potential of some species, especially Rosa canina and Sambucus nigra.
Article
Agronomy
Pavlina Drogoudi, Georgios Pantelidis, Lefkothea Karapetsi, Konstantina Ziakou, Konstantinos Kazantzis, Panagiotis Madesis, Thomas Thomidis
Summary: This study conducted a prospection of local traditional and underutilized peach genetic resources in Greece and investigated their genetic and morphological variations and susceptibility to shoot blight. A total of 32 peach cultivars/accessions were collected, and leaf and fruit morphological characterization was performed. The results showed that the local peach genotypes were different from foreign cultivars in several leaf and fruit phenotypic traits. Local cultivars exhibited greater resistance to Cylindrocarpon destructans, with certain cultivars showing the highest resistance. Genetic characterization revealed moderate to low genetic diversity among the peach cultivars, and several markers were found to be significantly correlated with fruit traits. These findings are valuable for breeding programs and future research on peach.
Article
Biochemistry & Molecular Biology
Nikolaos D. Georgakis, Elisavet Ioannou, Marianna Chatzikonstantinou, Marian Merino, Evangelia G. Chronopoulou, Jose Luis Mullor, Panagiotis Madesis, Nikolaos E. Labrou
Summary: In this study, the cosmeceutical properties of aqueous extracts from the microalgae strain Trachydiscus minutus were evaluated and compared with three widely used Chlorella strains. The T. minutus extracts showed the highest total antioxidant capacity and inhibitory potency towards elastase, indicating potential in controlling skin aging. The extracts also exhibited anti-inflammatory activity and protection against UVA-induced cell damage. The results suggest that T. minutus extract has promising cosmeceutical properties and potential for skincare use.
Review
Biochemistry & Molecular Biology
Alexandros Mavrommatis, Eleni Tsiplakou, Anastasia Zerva, Panagiota D. Pantiora, Nikolaos D. Georgakis, Georgia P. Tsintzou, Panagiotis Madesis, Nikolaos E. Labrou
Summary: Microalgae are a renewable source with beneficial effects on nutrition and health. They have been explored as feed additives in livestock animals, improving their health, product quality, and environmental impact. However, the cost-effective production and use of microalgae remains a challenge.
