Article
Nutrition & Dietetics
Qianhui Qi, Guohua Zhang, Wei Wang, Faizan Ahmed Sadiq, Yu Zhang, Xue Li, Qihe Chen, Qile Xia, Xinquan Wang, Yougui Li
Summary: This study compared and described the characteristics of soybeans at different germination stages. The findings showed that soybeans at 24 hours of germination had higher protein and isoflavone contents. The hydrolyzed protein from germinated soybeans exhibited higher antioxidant activity compared to soybean protein from soybean meal. The study also determined the optimal conditions for protein hydrolysis and identified the best fraction with antioxidant activity. Germination for 24 hours with a bud length of 0.5-1 cm was suggested as a special pretreatment for the development of germinated foods.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Applied
Mengmeng Zhang, Xuan Xin, Hui Wu, Hong Zhang
Summary: Proteases extracted from soybean seedlings germinated on the sixth day showed the highest activity and were able to effectively reduce the bitterness of soybean protein hydrolysates without compromising their antioxidant activity.
Article
Biochemistry & Molecular Biology
Jencia Carminha Colaco, Arun Pandian Chandrasekaran, Janardhan Keshav Karapurkar, Girish Birappa, Sripriya Rajkumar, D. A. Ayush Gowda, Bharathi Suresh, Junwon Lee, Vijai Singh, Seok-Ho Hong, Kye-Seon Kim, Suresh Ramakrishna
Summary: In this study, the researchers identified a membrane-bound carrier protein called SLC35F2, which is associated with multiple cancers. They found that the expression level of SLC35F2 is a key factor in cancer progression. They also discovered that STrCP1, an E3 ligase, interacts with and reduces the abundance of SLC35F2 protein in cancer cells. Depletion of STrCP1 led to an accumulation of SLC35F2 protein and promoted cancer cell growth, migration, invasion, and colony formation. Overall, they demonstrate that STrCP1 acts as a tumor suppressor by controlling SLC35F2 protein abundance in cancer cells, suggesting that STrCP1 could be a potential target for cancer therapeutics.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Food Science & Technology
Weixin Li, Lixia Mu, Yuxiao Zou, Weifei Wang, Haifeng Zhao, Xuli Wu, Sentai Liao
Summary: The study found that the hydrolysates from silkworm pupa proteins could inhibit the proliferation of MGC-803 gastric cancer cells, possibly by activating apoptosis.
Article
Food Science & Technology
Geng Li, Jingwen Xu, Huiwen Wang, Lianzhou Jiang, Huan Wang, Yan Zhang, Hua Jin, Zhijun Fan, Jing Xu, Qingshan Zhao
Summary: This study investigated the changes in structure and properties of soybean protein after hydrolysis using two types of hybrid nanoflowers. The results indicated that the hydrolysates treated with hybrid nanoflowers showed better solubility and antioxidant properties compared to soybean protein. Furthermore, the basic properties and oxidative stability of the soybean-protein-hydrolysates oil-in-water emulsions were improved. These findings highlight the importance of proteins hydrolyzed by hybrid nanoflowers as emulsifiers and antioxidants in the food and pharmaceutical industry.
Article
Chemistry, Applied
Lin Li, Hui He, Daize Wu, Derong Lin, Wen Qin, Demei Meng, Rui Yang, Qing Zhang
Summary: The research showed that incorporating SPI hydrolysates reduced the elastic modulus and hardness of SPI gel, especially with lower molecular weight hydrolysates. Glycosylation partially mitigated the adverse effects of hydrolysates on gel formation, but did not improve gel quality compared to the control. These results provide insights into the influencing mechanism of SPI hydrolysates and their glycosylated products on SPI gel formation.
Article
Food Science & Technology
Huaxing Sun, Liyu Liu, Weiwei He, Chenchen Cao, Xueying Song, Mouming Zhao, Jianan Zhang, Guowan Su
Summary: This study investigated the composition and factors affecting the formation of aggregates in concentrated soybean protein isolate hydrolysates (SPIH), as well as their impact on product quality. It was found that adding polysaccharide coagulants and aldose could effectively inhibit aggregate formation and enhance umami taste.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Carlotta Bollati, Ruoxian Xu, Giovanna Boschin, Martina Bartolomei, Fabrizio Rivardo, Jianqiang Li, Anna Arnoldi, Carmen Lammi
Summary: This study characterized the potential pleotropic activity of two commercially available soybean and pea protein hydrolysates, revealing their ability to inhibit DPP-IV and ACE activity. These findings suggest that these hydrolysates could be used in the development of innovative multifunctional foods and nutraceuticals for the prevention of metabolic syndrome.
Article
Biochemistry & Molecular Biology
Georgii V. Dolgalev, Taras A. Safonov, Viktoriia A. Arzumanian, Olga I. Kiseleva, Ekaterina V. Poverennaya
Summary: The improvement of proteomic techniques, especially mass spectrometry, has provided quantified proteomes with unprecedented depth and accuracy. However, there is still a significant difference in the reported numbers of total protein molecules per specific cell type. This article investigates proteomic studies of Escherichia coli, Saccharomyces cerevisiae, and HeLa cells in terms of total protein copy numbers per cell and finds up to a ten-fold difference between reported values. The study concludes that neither an unmeasured fraction of the proteome nor biases in protein quantification can explain the observed discrepancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jian Zhang, Qiyue Zhang, He Li, Xinwei Chen, Wanlu Liu, Xinqi Liu
Summary: This study aimed to purify and identify selenium-containing antioxidative peptides from selenium-enriched soybean peptides. The identified selenium-containing pentapeptide showed preventive effects against oxidative stress induced by hydrogen peroxide in HepG2 cells, demonstrating its potential as a natural antioxidative functional material for dietary supplementation.
Article
Pathology
Claudio Cordova, Carlo Lozano, Belen Rodriguez, Ivanny Marchant, Rodrigo Zuniga, Paola Ochova, Pablo Olivero, Wilfredo Alejandro Gonzalez-Arriagada
Summary: Optogenetics is a molecular biological technique involving transfection of cells with photosensitive proteins to study their biological effects. This study evaluated the impact of blue light on the survival of HeLa cells transfected with channelrhodopsin-2 (ChR2), revealing a significant correlation between the frequency and time of light stimulation and cell survival.
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Changhong Li, Linli Zhang, Chengmei Liu, Xuemei He, Mingshun Chen, Jun Chen
Summary: This study investigates the anti-cervical cancer activity of lipophilic grape seed proanthocyanidin (LGSP). LGSP showed a significant anti-proliferative effect on HeLa cells by increasing reactive oxygen species, inducing cell apoptosis, and blocking cell cycle progression. It also effectively suppressed the growth of a HeLa xenograft tumor in a zebrafish model.
Article
Agriculture, Multidisciplinary
Haining Guan, Yanli Tian, Dengyong Liu, Xiaoqin Diao, Chunmei Feng, Xiaojun Xu
Summary: This study investigates the effects of soybean protein isolate hydrolysates (SPIHs) produced at high hydrostatic pressure on the gelling properties, structural characteristics, and main forces of myofibrillar protein (MP) in plural gels. The results show that the addition of SPIHs improves the gel strength, water holding capacity, and structural properties of the plural gels.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Chemistry, Applied
Xiaoying Zhang, Yuyang Huang, Ruxin Ma, Yuqing Tang, Yang Li, Shuang Zhang
Summary: In this study, the effects of hydrolyzation with cell envelope proteinase (CEP) on the structure and biological properties of soybean protein isolate (SPI) were investigated. CEP hydrolysis resulted in the unfolding of soybean proteins and an increase in hydrophobic amino acids, leading to increased surface hydrophobicity and fluorescence of the soybean protein hydrolysates. The hydrolysates also exhibited enhanced antioxidant activity and potential biological activities such as angiotensin-converting enzyme inhibitory activity and dipeptidyl peptidase-IV inhibitory activity.
Article
Food Science & Technology
Chenchen Cao, Huaxing Sun, Xueying Song, Mouming Zhao, Weifeng Lin, Weizheng Sun, Lizhao Lin, Weixin Li, Guowan Su
Summary: This study focused on the impact of fermentation with Tetragenococcus halophilus and Zygosaccharomyces rouxii on the flavor characteristics of soy protein hydrolysates (SPH). Sensory evaluation, high performance liquid chromatography, and electronic tongue were used to analyze the sensory attributes and non-volatile taste compounds of SPHs. The results showed that fermentation significantly increased the content of certain amino acids and organic acids, thereby improving the acidity, umaminess, and sweetness taste of the samples. Principal component analysis and partial least square discriminant analysis confirmed the substantial influence of lactic acid bacteria and yeast fermentation on the non-volatile taste characteristics of SPHs.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Medicine, General & Internal
Pablo Romero-Morelos, Cindy Bandala, Julian Jimenez-Tenorio, Mariana Valdespino-Zavala, Miriam Rodriguez-Esquivel, Reyna Anaid Gama-Rios, Artfy Bandera, Monica Mendoza-Rodriguez, Keiko Taniguchi, Daniel Marrero-Rodriguez, Ricardo Lopez-Romero, Eva Ramon-Gallegos, Mauricio Salcedo
Article
Chemistry, Medicinal
Abraham Pucheta, Aaron Mendieta, Damian A. Madrigal, Roberto I. Hernandez-Benitez, Liseth Romero, Leticia Garduno-Siciliano, Catalina Rugerio-Escalona, Maria C. Cruz-Lopez, Fabiola Jimenez, Alejandra Ramirez-Villalva, Aydee Fuentes-Benites, Carlos Gonzalez-Romero, Omar Gomez-Garcia, Julio Lopez, Miguel A. Vazquez, Blanca Rosales-Acosta, Lourdes Villa-Tanaca, Alfonso Sequeda-Juarez, Eva Ramon-Gallegos, German Chamorro-Cevallos, Francisco Delgado, Joaquin Tamariz
MEDICINAL CHEMISTRY RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Sergio Andres Torres-Perez, Maria del Pilar Ramos-Godinez, Eva Ramon-Gallegos
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2020)
Review
Oncology
Sergio Andres Torres-Perez, Cindy Estefani Torres-Perez, Martha Pedraza-Escalona, Sonia Mayra Perez-Tapia, Eva Ramon-Gallegos
FRONTIERS IN ONCOLOGY
(2020)
Article
Oncology
Maria Guadalupe Cupido-Sanchez, Norma Estela Herrera-Gonzalez, Columba Citlalli Barrera Mendoza, Maria Luisa Morales Hernandez, Eva Ramon-Gallegos
Summary: This study compared the differential expression of microRNAs between untreated MDA-MB-231 cells and those surviving photodynamic therapy (PDT). The results showed a significant decrease in hsa-miR-16 expression in cells surviving PDT, which may have altered its downstream target network. Analysis also predicted a network related to cell cycle, proliferation, and apoptosis, suggesting an enhanced survival capacity in the treated population.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2021)
Review
Oncology
Alejandro Martinez-Escobar, Benjamin Luna-Callejas, Eva Ramon-Gallegos
Summary: Cancer treatment is challenging due to high resistance, but recent advancements in therapies have improved effectiveness and reduced toxic and invasive effects. Drug development for cancer is costly and time-consuming, but repurposed drugs combined with CRISPR-dCas9-based artificial transcription factors show potential for improving efficacy and reducing mortality.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Citlali Cecilia Mendoza-Guevara, Eva Ramon-Gallegos, Alejandro Martinez-Escobar, Rogelio Alonso-Morales, Maria del Pilar Ramos-Godinez, Jorge Ortega
Summary: Urban rabies in Mexico has been reduced through canine control and vaccination campaigns, but wild animal transmitted rabies, especially by vampire bats, has been increasing. This project presents an alternative for controlling rabies in bats using plasmid vaccines created through bio-nanotechnology.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2022)
Article
Biochemical Research Methods
Irving Trejo-Santillan, Citlali Cecilia Mendoza-Guevara, Maria del Pilar Ramos-Godinez, Eva Ramon-Gallegos
Summary: This study evaluated the biosafety of chitosan polymeric nanoparticles conjugated with protoporphyrin IX and vitamin B9. The conjugate showed no cytotoxic or genotoxic effects and even decreased chromosomal aberrations, thus improving genomic stability.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2022)
Article
Biochemical Research Methods
Irving Trejo-Santillan, Citlali Cecilia Mendoza-Guevara, Maria del Pilar Ramos-Godinez, Eva Ramon-Gallegos
Summary: One of the main obstacles in Photodynamic Therapy (PDT) is the aggregation of most photosensitizers (Ps) in aqueous solutions without specificity. Nanotechnology provides a new method to enhance the specificity towards target cells. This study aimed to optimize the synthesis of chitosan polymeric nanoparticles conjugated with protoporphyrin IX and vitamin B9 for improved efficiency.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2022)
Article
Oncology
Alina Serrano-Quintero, Alfonso Sequeda-Juarez, C. Angelica Perez-Hernandez, Sara M. Sosa-Delgado, Alfonso Mendez-Tenorio, Eva Ramon-Gallegos
Summary: This study identified two immunogenic peptides from calreticulin and HSP90 induced by PDT in MDA-MB-231 cells, showing potential as peptide-based vaccine for triple-negative breast cancer.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Maria Teresa Lopez-Cardenas, Adriana Jimenez, Araceli Espinosa-Montesinos, Elizabeth Maldonado-Alvarado, Martha Olivia Osorio-Peralta, Alejandro Martinez-Escobar, Alejandra Moreno-Vazquez, Ma. Guadalupe Aguilera-Arreola, Eva Ramon-Gallegos
Summary: Photodynamic therapy (PDT) showed high effectiveness in eliminating CIN I, HPV infection, and pathogenic microorganisms, indicating its potential as a promising therapy for cervical infections.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Benjamin Luna-Callejas, Laura Oropeza-Ramos, Eva Ramon-Gallegos
Summary: This study presents a comparative genomic analysis to identify target genes for designing LAMP primers and implements a LAMP assay for fast and accurate detection of Mycoplasma contamination in cell culture. The infB gene was found to be the best candidate for designing LAMP primers based on multiple sequence alignment. The LAMP assay at 63 degrees Celsius for 30 minutes showed 100% positive amplifications for detecting Mycoplasma species.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Lourdes A. Vega Rasgado, Eva Ramon-Gallegos, Lorena Rodriguez-Paez, Veronica Alcantara-Farfan
Summary: Nitric oxide (NO) is involved in processes such as vasodilation and neurotransmission/neuromodulation. Its role in epilepsy is controversial, as it can have anticonvulsant and proconvulsant properties. Understanding the dual effect of NO in seizures could lead to the development of new antiepileptic drugs. This study investigated the effects of convulsant drugs on neuronal and endothelial NO levels.
ACTA PHARMACEUTICA
(2023)
Article
Oncology
Alfonso Sequeda-Juarez, Adriana Jimenez, Araceli Espinosa-Montesinos, Maria del Carmen Cardenas-Aguayo, Eva Ramon-Gallegos
ANTICANCER RESEARCH
(2020)
Article
Engineering, Electrical & Electronic
Roberto G. Ramirez-Chavarria, Celia Sanchez-Perez, Luisa Romero-Ornelas, Eva Ramon-Gallegos
Summary: Impedance measurement is a common technique used for detecting the electrical properties of biological cells. This study introduces a label-free method for sensing biological cells based on impedance and the DRT model, which maps impedance data to a time-constant-domain spectrum for data analysis, providing a high sensitivity and resolution electrical fingerprint for the sample.
IEEE SENSORS JOURNAL
(2021)