Article
Plant Sciences
Wenting Li, Zhenxi Li, Jiaquan Huang, Meirong Xu, Zheng Zheng, Xiaoling Deng
Summary: This study provides a reliable CRISPR/cas system for Xcc strains and identifies 15 types of CRISPR arrays with 25 spacers in XccA strains from six provinces in China. The phylogenetic analysis based on the CRISPR locus shows a more reliable and accurate typing result compared to commonly used loci. In addition, seven associated cas genes are found adjacent to the CRISPR array.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Multidisciplinary Sciences
Paola E. Campos, Olivier Pruvost, Karine Boyer, Frederic Chiroleu, Thuy Trang Cao, Myriam Gaudeul, Claudia Baider, Timothy M. A. Utteridge, Nathalie Becker, Adrien Rieux, Lionel Gagnevin
Summary: Herbarium collections provide valuable DNA resources for studying the evolutionary history of plant pathogens. This study reconstructs historical genomes of the bacterial crop pathogen Xanthomonas citri pv. citri (Xci) from Citrus herbarium specimens and compares them with modern genomes to understand its origin and diversification.
NATURE COMMUNICATIONS
(2023)
Article
Horticulture
Ashok Kumar Mahawer, Anil Kumar Dubey, Om Prakash Awasthi, Dinesh Singh, Anil Dahuja, Amitha Mithra Sevanthi, Amrender Kumar, Amit Kumar Goswami, Nimisha Sharma, Jagdish Yadav, Amit Kumar Kesharwani, Abhijeet Shankar Kashyap, Aditya Kulshreshtha, Ravinder Pal Singh, Amrut Morade, Radha Mohan Sharma
Summary: India is a leading citrus-producing country with significant production of lemons and limes. However, its contribution to the global citrus market is limited due to factors like citrus canker. This study investigated the impact of Xanthomonas citri pv. citri on different Citrus spp. and found that susceptible genotypes showed greater degradation in chlorophyll content and reduction in leaf gas exchange parameters compared to resistant genotypes. Resistant genotypes also exhibited higher activities of antioxidant enzymes and phenolics. The results provide insights into the pathogen mechanism and resistance in Citrus species.
Article
Biochemistry & Molecular Biology
Mei Zhu, Yan Li, Xuesha Long, Congyu Wang, Guiping Ouyang, Zhenchao Wang
Summary: Compound 2-(butyldisulfanyl) quinazolin-4(3H)-one exhibited strong anti-Xac activity and effectively controlled citrus canker. It reduced biofilm formation, increased reactive oxygen species levels, damaged bacterial morphology, and caused bacterial death. Through down-regulation of enzymes in the MEP pathway, this compound showed destructive ability against Xac. Overall, this study suggests that such derivatives hold promise as novel bactericides for citrus canker control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Muhammad Nauman, Sidra Mushtaq, Muhammad Fahad Khan, Amjad Ali, Syed Atif Hasan Naqvi, Zia Ul Haq, Muhammad Asif Zulfiqar, Ateeq Ur Rehman, Mahmoud Moustafa, Ummad Ud Din Umar
Summary: This study collected citrus canker infected samples from different citrus orchards in Punjab and isolated the pathogen Xanthomonas citri subsp. citri (Xcc). The pathogenicity of Xcc was confirmed through attached and detached leaf assays, and its hypersensitive response was confirmed on tobacco plants. The isolated Xcc was characterized using molecular methods and revealed significant variation. Phylogenetic analysis showed that the Pakistani isolates are closely related to isolates from Japan, India, and Spain, but distantly related to isolates from Brazil and USA.
PAKISTAN JOURNAL OF BOTANY
(2023)
Article
Microbiology
K. Dineshkumar, Ginny Antony
Summary: In this study, using the genome subtraction approach, we identified 69 possible drug target proteins in Xanthomonas citri pv. punicae (Xcp). Further analysis revealed 33 potential virulent proteins, including two iron complex outer membrane receptors and a T4SS PilQ protein. These outer membrane-localized proteins could serve as candidate targets for antibacterial agents, and the two iron complex outer membrane receptor proteins show homology with the Drug Bank listed drug target sequences. We also identified PilQ as a novel antimicrobial target of Xcp and analyzed its protein-protein interacting partners and phylogenetic relatedness.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Plant Sciences
Wenting Li, Zhenxi Li, Ling Zhang, Xiaoling Deng, Zheng Zheng
Summary: This study presents the genome sequence of the oldest X. citri pv. citri strain from historical citrus canker specimens in China, which will contribute to the genomic evolution research of X. citri pv. citri.
Article
Biochemistry & Molecular Biology
Nicoletta Pucci, Valeria Scala, Giuseppe Tatulli, Alessia L'Aurora, Simone Lucchesi, Manuel Salustri, Stefania Loreti
Summary: This study evaluated the influence of four different DNA extraction methods on the reliability of detecting citrus bacterial canker. The droplet digital PCR method was found to be more effective based on the comparison of real-time PCR standard curves and the evaluation of performance criteria of spiked samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Doron Teper, Jin Xu, Sheo Shankar Pandey, Nian Wang
Summary: Citrus canker disease caused by Xanthomonas citri subsp. citri is a destructive disease in citrus, with different pathotypes displaying varying host ranges. This study investigated the adaptation of Xcc(AW) strains to incompatible sweet orange, revealing increased expression of type III secretion system and effector genes in adapted strains. Mutations in pthAW1 were identified as contributing to the adaptation of Xcc(AW) to sweet orange, demonstrating active rearrangements of the TALE repeat array during host adaptation.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Biotechnology & Applied Microbiology
R. Ragupathy, K. A. Jolley, C. Zamuner, J. B. Jones, J. Redfern, F. Behlau, H. Ferreira, M. C. Enright
Summary: Xanthomonas citri subsp. citri is a major plant pathogen that causes bacterial citrus canker, resulting in substantial economic losses to the citrus industry. Genomic sequencing has provided insights into the evolutionary relationships and distinct pathotypes of X. citri subsp. citri. The core-genome multilocus sequence typing (cgMLST) scheme described in this study will be a valuable resource for studying the epidemiology and evolution of this plant-pathogenic bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Kaihuai Li, Jinxing Liao, Ming Wei, Shanxu Qiu, Weiyin Wu, Yancun Zhao, Haihong Wang, Qiongguang Liu, Fengquan Liu, Changqing Chang
Summary: This study found that low osmostress can reverse the growth and virulence deficiencies of X. citri.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
In-Jeong Kang, Kyung Seok Kim, Gwyn A. Beattie, Jung-Wook Yang, Kee Hoon Sohn, Sunggi Heu, Ingyu Hwang
Summary: Analysis of pathogenicity genes of Xanthomonas citri pv. glycines strains from Korea and the United States revealed differences in candidate type III secretion system effector (T3SE) genes and diverse repeat sizes of transcription activator-like effectors (TALEs). Phylogenetic analysis showed two distinct clusters of TALEs with 17.5 repeats, suggesting adaptation to different soybean cultivars. The evolving target specificity of TALEs' binding domains in soybean cultivars enhances understanding of Xcg pathogenicity.
Article
Agronomy
Helvio Gledson Maciel Ferraz, Jorge Luis Badel, Yane Fernandes Neves, Ana Carolina Lopes Eloi, Pedro Marcus Pereira Vidigal, Lucio Mauro da Silva Guimaraes, Acelino Couto Alfenas
Summary: The causal agent of bacterial leaf blight (BLB) in eucalypt plants in Brazil has been identified as Xanthomonas spp., with high genetic diversity. The transfer of Xanthomonas axonopodis pv. eucalyptorum to Xanthomonas citri is proposed. These findings are crucial for the management of eucalypt BLB in nurseries and fields.
Article
Biotechnology & Applied Microbiology
A. Sampathkumar, K. Eraivan Arutkani Aiyanathan, S. Nakkeeran, S. Manickam
Summary: Bacterial antagonists were isolated from cotton plant associated bacteria and evaluated for their efficacy against X. citri pv. malvacearum in vitro. Bacillus amyloliquefaciens strain ETL2 was found to be highly effective in inhibiting bacterial growth, followed by Bacillus velezensis strain ELS2. PCR amplification of antimicrobial peptide (AMP) genes revealed that B. amyloliquefaciens strain ETL2 had the highest number of antibiotic biosynthesis genes, and GC-MS analysis confirmed the presence of aromatic hydrocarbons, phenols, and fatty acids in the culture extracts of ETL2. Field experiments showed that seed treatment and foliar spray with ETL2 and ELS2 resulted in optimal plant growth and cotton yield, as well as effective disease control.
BIOLOGICAL CONTROL
(2023)
Article
Microbiology
Filip Gazdik, Samuel Magnus, Steven J. Roberts, Rafal Baranski, Jana Cechova, Robert Pokluda, Ales Eichmeier, Dariusz Grzebelus, Miroslav Baranek
Summary: This study aimed to monitor the persistence of Xanthomonas campestris pv. campestris (Xcc) in field soil in central Europe, using different PCR assays. It was found that Xcc can persist in soil for up to two years, and the nested real-time PCR method showed higher sensitivity in detecting Xcc in soil compared to conventional PCR.
News Item
Plant Sciences
D. Richard, C. Boyer, S. Javegny, K. Boyer, P. Grygiel, O. Pruvost, A. L. Rioualec, V. Rakotobe, J. Iotti, R. Picard, C. Verniere, C. Audusseau, C. Francois, V. Olivier, A. Moreau, A. Chabirand
Article
Plant Sciences
C. Zombre, P. Sankara, S. L. Ouedraogo, I. Wonni, K. Boyer, C. Boyer, M. Terville, S. Javegny, A. Allibert, C. Verniere, O. Pruvost
Review
Biochemistry & Molecular Biology
D. Richard, V. Ravigne, A. Rieux, B. Facon, C. Boyer, K. Boyer, P. Grygiel, S. Javegny, M. Terville, B. I. Canteros, I. Robene, C. Verniere, A. Chabirand, O. Pruvost, P. Lefeuvre
News Item
Plant Sciences
C. Zombre, I. Wonni, S. L. Ouedraogo, K. E. Kpemoua, K. Assignon, P. Sankara, C. Verniere, C. Boyer, K. Boyer, S. Javegny, O. Pruvost
News Item
Plant Sciences
D. Richard, N. Tribot, C. Boyer, M. Terville, K. Boyer, S. Javegny, M. Roux-Cuvelier, O. Pruvost, A. Moreau, A. Chabirand, C. Verniere
Article
Plant Sciences
Thomas Chesneau, Geraldine Maignien, Claudine Boyer, Jean-Jacques Cheron, Michel Roux-Cuvelier, Luc Vanhuffel, Stephane Poussier, Philippe Prior
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Biochemical Research Methods
Emmanuel Jouen, Frederic Chiroleu, Veronique Maillot-Lebon, Aude Chabirand, Sabine Merion, Claudine Boyer, Olivier Pruvost, Isabelle Robene
JOURNAL OF MICROBIOLOGICAL METHODS
(2019)
Article
Biochemistry & Molecular Biology
Damien Richard, Olivier Pruvost, Francois Balloux, Claudine Boyer, Adrien Rieux, Pierre Lefeuvre
Summary: Horizontal gene transfer plays a key role in the evolution by redistributing phenotypically important genes among lineages. Studies on a bacterial plant pathogen lineage demonstrated extensive gene content variation among lineages even at small phylogenetic and geographic scales. Accessory genes are preferentially located on plasmids, while chromosomal regions also play a role in hosting ecologically important genes.
Article
Multidisciplinary Sciences
Angelina Trotereau, Claudine Boyer, Isabelle Bornard, Max Jean Bernard Pecheur, Catherine Schouler, Clara Torres-Barcelo
Summary: This study presents the full genomes of 23 new phages infecting RSSC isolated from agricultural samples in Mauritius and Reunion islands, revealing high genetic diversity between them and weak similarities with previous related phages. The results support the proposal of 13 new species and seven new genera of R. solanacearum phages. The findings highlight the wide prevalence of phages of RSSC in infected agricultural settings and the underlying genetic diversity.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Olivier Pruvost, Damien Richard, Karine Boyer, Stephanie Javegny, Claudine Boyer, Frederic Chiroleu, Pierre Grygiel, Evelyne Parvedy, Isabelle Robene, Veronique Maillot-Lebon, Azali Hamza, Kanta Kumar Lobin, Marc Naiken, Christian Verniere
Summary: A comprehensive study on X. citri pv. citri in the South West Indian Ocean (SWIO) region revealed a new genetically unrelated pathotype A* and diverse strains in the Seychelles. The study also showed that strains from the Seychelles formed three different clusters, indicating higher diversity compared to the Comoros and the Mascarenes.
Article
Microbiology
Grazia Licciardello, Paola Caruso, Patrizia Bella, Claudine Boyer, Malcolm W. Smith, Olivier Pruvost, Isabelle Robene, Jaime Cubero, Vittoria Catara
Summary: This study evaluated the susceptibility of 32 ornamental Rutaceae plants to Xcc and Xca, causal agents of citrus bacterial canker. The results showed high variability in symptoms and bacterial population among different strains and hosts, indicating complex host-pathogen interactions. The findings support the need to regulate non-citrus Rutaceae plant introductions into areas currently free of this economically important pathogen.
Editorial Material
Plant Sciences
F. Pilet, M. Loiseau, C. Boyer, A. Cavalier, C. Diman
Article
Microbiology
Damien Richard, Adrien Rieux, Pierre Lefeuvre, Azali Hamza, Kanta Kumar Lobin, Mark Naiken, Randy Stravens, Claudine Boyer, Karine Boyer, Stephanie Javegny, Olivier Pruvost
Summary: High-quality Illumina assemblies were produced from 284 strains of Xanthomonas citri pv. citri pathotype A, mainly from the Southwest Indian Ocean region, with some strains also sequenced using MinION technology. It was found that some strains of this bacterium hosted chromosomally encoded transcription activator-like effector (TALE) genes, which is an atypical feature.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Microbiology
Damien Richard, Claudine Boyer, Christian Verniere, Blanca I. Canteros, Pierre Lefeuvre, Olivier Pruvost
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Damien Richard, Claudine Boyer, Pierre Lefeuvre, Blanca I. Canteros, Shyam Beni-Madhu, Perrine Portier, Olivier Pruvost
GENOME ANNOUNCEMENTS
(2017)