Review
Plant Sciences
Sarada D. Tetali, Satyabrata Acharya, Aditya B. Ankari, Vadthyavath Nanakram, Agepati S. Raghavendra
Summary: Withania somnifera, commonly known as Indian ginseng, has been extensively used in Ayurveda for various health benefits. Metabolomic studies have shown that the metabolite profiles of W. somnifera vary depending on plant parts collected, developmental stage, season, and geographical location. The use of sophisticated techniques like LC-MS, GC-MS, HPTLC, and NMR has facilitated the discovery of new metabolites and comprehensive metabolomic profiles.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Vivek K. Kashyap, Godwin Peasah-Darkwah, Anupam Dhasmana, Meena Jaggi, Murali M. Yallapu, Subhash C. Chauhan
Summary: This review highlights the potential of Withania somnifera (WS) as an anticancer and immunomodulatory molecule. Several preclinical studies have shown that WS can prevent or slow the progression of cancer originating from various organs. However, stability, bioavailability, and target specificity are major obstacles in the application of WS for cancer therapy.
Review
Pharmacology & Pharmacy
Arsalan Bashir, Masarat Nabi, Nahida Tabassum, Suhaib Afzal, Mehrose Ayoub
Summary: Ashwagandha, as a significant medicinal plant, has been used in Ayurvedic and indigenous systems of medicine for over 3,000 years. It contains bioactive constituents known as withanolides, which have a broad spectrum of therapeutic potential. Research has shown that Ashwagandha can combat various chronic diseases and has effects on multiple molecular targets.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Medicine, Research & Experimental
Subhabrata Paul, Shreya Chakraborty, Uttpal Anand, Swarnali Dey, Samapika Nandy, Mimosa Ghorai, Suchismita Chatterjee Saha, Manoj Tukaram Patil, Ramesh Kandimalla, Jaroslaw Prockow, Abhijit Dey
Summary: Withania somnifera, a traditional Rasayana herb, exhibits potent pharmacological activities against a variety of human medical conditions. Further studies are needed to explore its clinical applications and toxicological properties for safe and efficacious use as a health-promoting herb.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Pukar Khanal, Rupesh Chikhale, Yadu Nandan Dey, Ismail Pasha, Sharad Chand, Nilambari Gurav, Muniappan Ayyanar, B. M. Patil, Shailendra Gurav
Summary: Traditionally used as an immune booster and anti-viral, Withania somnifera contains withanolides which have shown promising potential against COVID-19. Withanolide_G, Withanolide_I, and Withanolide_M from Withania somnifera exhibited the highest binding affinity with PLpro, 3CLpro, and spike protein, respectively. The findings suggest that withanolides could be effective in boosting the immune system and inhibiting COVID-19 infection.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Plant Sciences
Seoung Rak Lee, Bum Soo Lee, Jae Sik Yu, Heesun Kang, Min Jeong Yoo, Sang Ah Yi, Jeung-Whan Han, Sil Kim, Jung Kyu Kim, Jin-Chul Kim, Ki Hyun Kim
Summary: This study identifies withasilolides from Indian ginseng as potential anti-adipogenic compounds for the treatment of obesity and related metabolic diseases.
JOURNAL OF GINSENG RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Kuldeep Kaur, Dechen Dolker, Shashikanta Behera, Pratap Kumar Pati
Summary: This review discusses the advancements in in vitro propagation and secondary metabolites biosynthesis of Withania somnifera.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Sachin Ashok Thorat, Arya Kaniyassery, Poornima Poojari, Melissa Rangel, Shashikala Tantry, Kodsara Ramachandra Kiran, Manjunath B. Joshi, Padmalatha S. Rai, Anna-Maria Botha, Annamalai Muthusamy
Summary: This study investigated the influence of plant growth regulators on the growth and biosynthesis of bioactive compounds in Ashwagandha at different growth stages. The results show that plant growth regulators can promote plant growth and the synthesis of important bioactive compounds like withanolides.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agricultural Engineering
Kuldeep Kaur, Pritika Singh, Kulwinder Kaur, Abhishek Bhandawat, Panchsheela Nogia, Pratap Kumar Pati
Summary: Withania somnifera is a medicinal plant with immense pharmaceutical potential. In vitro propagation system is used to produce genetically and phytochemically uniform plants, with a multi-step protocol developed to minimize the adverse effects of cytokinin. The protocol also includes modulation of ammonium nitrate strength for in vitro rooting initiation.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Nutrition & Dietetics
Helen Holvoet, Dani M. Long, Alexander Law, Christine McClure, Jaewoo Choi, Liping Yang, Luke Marney, Burkhard Poeck, Roland Strauss, Jan F. Stevens, Claudia S. Maier, Amala Soumyanath, Doris Kretzschmar
Summary: Withania somnifera (WS) extracts, widely used in traditional medicine and as nutritional supplements, have shown positive effects on healthy aging, sleep, and resilience against stress. Different extracts may have different beneficial effects due to the variation in active ingredients.
Article
Agricultural Engineering
Prince Choyal, Maharishi Tomar, Virendra Singh Rana, Manish Kumar Suthar, Kailashpati Tripathi, Kuldeepsingh A. Kalariya, Ponnuchamy Manivel, Bhupinder Singh
Summary: The study showed that altering the source-sink relationship can effectively increase root yields. Chemical gametocide and mechanical deflowering significantly increased root biomass and withanolides content.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Manali Singh, Sanjeev Agrawal, Obaid Afzal, Abdulmalik S. A. Altamimi, Alya Redhwan, Nawaf Alshammari, Mitesh Patel, Mohd Adnan, Abdelbaset Mohamed Elasbali, Shahanavaj Khan
Summary: This study aimed to optimize the production of withanolides in Withania somnifera by foliar spraying different elicitors. The results showed that the use of jasmonic acid and chitosan significantly increased the content of withanolides. This low-cost and eco-friendly approach can be a feasible way for farmers to enhance the production of withanolides.
Article
Biochemistry & Molecular Biology
Aditi Srivastava, Sahabjada Siddiqui, Rumana Ahmad, Sudhir Mehrotra, Bilal Ahmad, A. N. Srivastava
Summary: This study evaluated the antiviral potential of Withania somnifera phytoconstituents against SARS-CoV-2 using computational methods. The results showed that these phytoconstituents exhibited strong binding to viral proteins and receptors, with no toxic side effects. Further in vitro and in vivo investigations are warranted to explore the potential of these phytoconstituents as novel antiviral agents against COVID-19.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Agricultural Engineering
Kuldeep Kaur, Kulwinder Kaur, Abhishek Bhandawat, Pratap Kumar Pati
Summary: This study successfully selected an elite accession of Withania somnifera with high withaferin A content and utilized meta-Topolin (mT) as a novel cytokinin for aseptic cultures, achieving clonal mass propagation of shoots. Additionally, an effective regeneration protocol using leaf explant of W. somnifera for in vitro mass propagation and genetic transformation studies was developed. The genetic uniformity of the in vitro regenerated shoots was confirmed using RAPD, ISSR, and SCoT markers.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Integrative & Complementary Medicine
Getaye Tessema Desta, Yared Andargie Ferede, Woretaw Sisay Zewdu, Muluken Adela Alemu
Summary: This study evaluated the antidiarrheal activity of Withania somnifera leaves and found that the crude extract and solvent fractions of the plant can delay the onset of diarrhea and reduce the number and weight of stools.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
So Young Jeong, Hyomin Ahn, Jahee Ryu, Youngbin Oh, Ganeshan Sivanandhan, Kang-Hee Won, Young Doo Park, Jin-Soo Kim, Hyeran Kim, Yong Pyo Lim, Sang-Gyu Kim
PLANT BIOTECHNOLOGY REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Veda Krishnan, Joshna Jose, Monica Jolly, T. Vinutha, Raja Kumar, Markandan Manickavasagam, Shelly Praveen, Archana Sachdev
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Plant Sciences
Dorairaj Sathish, Jeevaraj Theboral, Venkatachalam Vasudevan, Gadamchetty Pavan, Chandrasekaran Ajithan, Chinnaswamy Appunu, Markandan Manickavasagam
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2020)
Article
Plant Sciences
Venkatachalam Vasudevan, Ramamoorthy Siva, Veda Krishnan, Markandan Manickavasagam
SOUTH AFRICAN JOURNAL OF BOTANY
(2020)
Article
Biotechnology & Applied Microbiology
Sivabalan Karthik, Gadamchetty Pavan, Markandan Manickavasagam
PLANT CELL TISSUE AND ORGAN CULTURE
(2020)
Article
Biochemical Research Methods
S. Dharshini, V. M. Manoj, G. S. Suresha, J. Ashwin Narayan, T. S. Sarath Padmanabhan, Ravinder Kumar, Mintu Ram Meena, M. Manickavasagam, Bakshi Ram, C. Appunu
PLANT MOLECULAR BIOLOGY REPORTER
(2020)
Article
Agricultural Engineering
Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, Yong Pyo Lim
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Plant Sciences
Sivabalan Karthik, Gadamchetty Pavan, Arumugam Prasanth, Selvam Sathish, Chinnaswamy Appunu, Markandan Manickavasagam
Summary: In this study, a new protocol for genetic transformation of bitter gourd plants in planta using seeds as target material was developed, with improved transformation efficiency achieved through vacuum infiltration and sonication. The protocol allows for the genetic engineering of bitter gourd plants with disease and pest-resistant traits.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Biotechnology & Applied Microbiology
Venkatachalam Vasudevan, Dorairaj Sathish, Chandrasekaran Ajithan, Selvam Sathish, Markandan Manickavasagam
Summary: The study developed an efficient, cost-effective, and rapid transformation method by infecting half-seed explants with Agrobacterium, which can produce a large number of transgenic watermelon lines within 56 days without involving any tissue culture phases.
PLANT BIOTECHNOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Selvam Sathish, Venkatachalam Vasudevan, Sivabalan Karthik, Chandrasekaran Ajithan, Ramamoorthy Siva, Shanthi Pandurengan Parthasarathy, Markandan Manickavasagam
Summary: The use of green synthesized AgNPs enhanced the regeneration frequency of Hybanthus enneaspermus plants and maintained genetic uniformity among the regenerated plants. This micropropagation protocol provides a new approach for targeted gene editing and functional gene analysis in this medicinally significant plant.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Plant Sciences
Chandrasekaran Ajithan, Venkatachalam Vasudevan, Selvam Sathish, Gadamchetty Pavan, Elangovan Yamini, Markandan Manickavasagam
Summary: An effective protocol for callus-dependent indirect organogenesis in Indian pea cv. Ageta 6 was standardized using cotyledonary node explant. The study highlighted the positive effects of polyamines and plant growth regulators on pea regeneration, and demonstrated that polyamine-assisted regenerated pea plants exhibited improved physiological and antioxidant profiles.
PLANT PHYSIOLOGY REPORTS
(2022)
Article
Plant Sciences
Sivabalan Karthik, Selvam Sathish, Jesudass Joseph Sahayarayan, Markandan Manickavasagam
Summary: In this study, a novel plant hormone called Meta-topolin (mT) was found to enhance regeneration and genetic transformation efficiency in radish. By adding mT in optimal concentrations to the culture medium, shoot induction, elongation, and rooting abilities of radish were improved. Using Agrobacterium-mediated transformation, mT also increased the efficiency of genetic transformation in radish.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Biotechnology & Applied Microbiology
Dhandapani Elayaraja, Kondeti Subramanyam, Venkatachalam Vasudevan, Selvam Sathish, Sampath Kasthurirengan, Andy Ganapathi, Markandan Manickavasagam
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2019)
Article
Horticulture
Ganeshan Sivanandhan, Sheng Bean Choi, Moon Jiae, Su Ryun Choi, Sang Gyu Kim, Young Doo Park, Yong Pyo Lim
HORTICULTURAL SCIENCE & TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Sivabalan Karthik, Gadamchetty Pavan, Veda Krishnan, Selvam Sathish, Markandan Manickavasagam
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2019)
