Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 154, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.indcrop.2020.112706
Keywords
Hairy root culture; Squalene synthase; Up-regulation; Withanolides; Withania somnifera
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Funding
- Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT, South Korea [2017H1D3A1A01054325]
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) through the Golden Seed Project - Ministry of Agriculture, Food and Rural Affairs (MAFRA) [213006-05-4-SB110]
- Ministry of Oceans and Fisheries (MOF)
- Rural Development Administration (RDA)
- Korea Forest Services (KFS)
- Chungnam National University, Daejeon, South Korea
- National Research Foundation of Korea [2017H1D3A1A01054325] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The roots of Indian ginseng, Withania somnifera (L.) Dunal (Solanaceae) are extensively used in ayurvedic and other traditional medicines. The plant produces a steroidal lactone, withanolide which has multiple health benefits. Squalene synthase plays a central role in regulating the withanolide biosynthetic pathway in W. somnifera. The present approach investigated the up-regulation of squalene synthase in withanolides biosynthesis pathway in hairy roots. The higher expression of squalene synthase was noticed in hairy root line Ws9 which exhibited withanolide A (2.82 mg/g DW), withanolide B (1.34 mg/g DW), withaferin A (1.83 mg/g DW) and withanone (1.97 mg/g DW) on the 40th day of culture with 1.08 to 1.25-times higher, respectively. Semi-quantitative and quantitative polymerase chain reaction confirmed the incorporation and expression of squalene synthase in cultured hairy roots.
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