Article
Plant Sciences
Milica D. Bogdanovic, Katarina B. Cukovic, Angelina R. Subotic, Milan B. Dragicevic, Ana D. Simonovic, Biljana K. Filipovic, Sladana I. Todorovic
Summary: Somatic embryogenesis (SE) is a crucial method for plant propagation in vitro, which can be induced from different tissues and organs. Research shows that inducing secondary SE at specific concentrations is beneficial for obtaining more and better somatic embryos.
Article
Agronomy
Hayriye Yildiz Dasgan, Sevda Kacmaz, Bekir Buelent Arpaci, Boran Ikiz, Nazim S. S. Gruda
Summary: The study aims to partially replace intensive mineral fertilizers with bio-fertilizers by supplementing 50% of the mineral fertilizers with Chlorella vulgaris microalgae, beneficial bacteria, and mycorrhiza. The research findings demonstrate that the use of bio-fertilizers significantly improves the quality of hydroponically grown baby spinach and reduces reliance on mineral fertilizers.
Article
Biochemistry & Molecular Biology
Intan Ernieza Farhana Nizan, Katialisa Kamaruddin, Pei-Wen Ong, Zubaidah Ramli, Rajinder Singh, Ray J. Rose, Pek-Lan Chan
Summary: EgENOD93 plays a significant role in somatic embryo formation in oil palm and Medicago truncatula. Overexpression of EgENOD93 in Arabidopsis results in enhanced embryogenic potential and increased shoot regeneration.
MOLECULAR BIOTECHNOLOGY
(2022)
Article
Forestry
Momi Tsuruta, Tsuyoshi E. Maruyama, Saneyoshi Ueno, Takumi Kaneeda, Yoshinari Moriguchi
Summary: This study provides basic information on somatic embryo maturation, germination, and growth performance of pollen-free sugi somatic plantlets derived from different embryogenic cell lines (ECLs). Significant differences in growth rate among ECLs were observed, indicating varying abilities in somatic seedling development.
Article
Forestry
Joao Paulo de Morais Oliveira, Natalia Arruda Sanglard, Adesio Ferreira, Wellington Ronildo Clarindo
Summary: Different genotypes of Coffea arabica exhibit distinct responses in vitro and can experience somaclonal variation. Epigenetic mechanisms, such as global cytosine methylation, play a role in influencing Coffea in vitro responses. During indirect somatic embryogenesis, the methylated cytosine levels can affect cell competence and somatic embryos regeneration, with different genotypes showing varied levels of methylation.
Article
Biotechnology & Applied Microbiology
Sara Hernandez-Castellano, Mauricio Andrade-Marcial, Edder D. Aguilar-Mendez, Victor M. Loyola-Vargas, Stefan de Folter, Clelia De-la-Pena
Summary: The study found that certain miRNAs play a crucial role in differentiation and stage development during somatic embryo development in coffee, influencing relevant genes involved in cell division and differentiation to enhance plant cellular totipotency.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Horticulture
Snezana Zdravkovic-Korac, Maja Belic, Dusica Calic, Jelena Milojevic
Summary: A spinach-supplemented diet offers various health benefits, but caution is necessary due to the potential health issues from high levels of oxalic acid and nitrate. Breeding can increase the levels of vitamins and phytochemicals, but conventional breeding is constrained by complex sex determination. By utilizing tissue culture techniques and biotechnological methods, limitations in spinach somatic embryogenesis can be overcome, providing a pathway for further research and improvement in the efficiency of this process.
Article
Plant Sciences
Eveline Y. Y. Kong, Julianne Biddle, Sundaravelpandian Kalaipandian, Steve W. W. Adkins
Summary: This study attempted to obtain friable coconut embryogenic callus (EC) through various approaches, but none of them were successful. However, some of the culture conditions were found to influence the formation of compact EC, which is important for further studies on somatic embryogenesis in coconut and other species.
Article
Plant Sciences
Charu Sharma, Papiya Mukherjee
Summary: This review summarizes the latest information on biotechnological prospects such as plant, cell, tissue, and organ culture, cryopreservation, and secondary metabolites production in the genus Saussurea. These techniques are crucial for the conservation of this plant and the production of its specialized metabolites.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Review
Plant Sciences
Xuan Sun, Xue Wang, Bijaya Sharma Subedi, Yin Jiang, Di Wang, Rongxin Gou, Guojun Zhang, Wenting Xu, Zunzheng Wei
Summary: This article introduces a plant called calla lily, which is native to tropical regions of Africa and has gained popularity in the international market due to its intricate morphology and prolonged flowering duration. However, there are challenges in hybrid production for two sub-groups of calla lily, known as group Zantedeschia and group Aestivae, due to 'plastome-genome incompatibility'. The article provides a comprehensive summary of calla lily tissue culture research, focusing on the regeneration pathways and factors influencing de novo organogenesis and somatic embryogenesis.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Hongbing She, Zhaosheng Xu, Helong Zhang, Guoliang Li, Jian Wu, Xiaowu Wang, Ying Li, Zhiyuan Liu, Wei Qian
Summary: Research on spinach dioecy identified a male-specific region on chromosome 4, potentially linked to the male-determination gene. A KASP marker, SponR, developed from a SNP closely linked to the MSR, co-segregated with the sex determination gene in a population of approximately 958 individuals.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Horticulture
Jiaqi Zou, Xiao Zou, Zhichao Gong, Gengxing Song, Jie Ren, Hui Feng
Summary: Curly kale, known for its rich nutrition and health benefits, is highly sought after and considered one of the healthiest vegetables. However, conventional breeding methods for this vegetable take a long time to produce homozygous inbred lines. In this study, the isolated microspore culture (IMC) technique was successfully used to accelerate the breeding cycle of curly kale through efficient cytokinin thidiazuron (TDZ) treatment. The results showed that 0.2 mg/L TDZ had the optimal effect on embryo survival and plant regeneration, significantly shortening the breeding cycle.
Article
Plant Sciences
Meiping Li, Dan Wang, Xiaofei Long, Zhaodong Hao, Ye Lu, Yanwei Zhou, Ye Peng, Tielong Cheng, Jisen Shi, Jinhui Chen
Summary: This study established and optimized a highly efficient genetic transformation system for Liriodendron hybrid using Agrobacterium-mediated transformation. By investigating various factors, the study successfully inserted functional genes into embryogenic calli and achieved plant regeneration and selection. The results demonstrated successful expression and integration of the inserted genes into the Liriodendron hybrid genome.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Asunta Mukami, Bicko Steve Juma, Cecilia Mweu, Mathew Ngugi, Richard Oduor, Wilton Mwema Mbinda
Summary: This study evaluated factors influencing protoplast isolation, callus induction, and plant regeneration in cassava, and developed a simple and highly reproducible protocol.
Article
Plant Sciences
Yekti Asih Purwestri, Yang-Seok Lee, Cathal Meehan, Windi Mose, Febri Adi Susanto, Putri Wijayanti, Anisa Nazera Fauzia, Tri Rini Nuringtyas, Nosheen Hussain, Hadi Lanang Putra, Jose Gutierrez-Marcos
Summary: Plants have the unique ability to form embryos from both gametes and somatic cells. The RWP-RK domain-containing protein (RKD) transcription factors play a crucial role in germ cell differentiation and embryo development in land plants. In this study, the overexpression of the rice RKD3 (OsRKD3) was found to induce somatic embryogenesis in a resistant black rice landrace. The activation of OsRKD3 led to the modulation of an extensive gene network involved in hormone signal transduction, stress responses, and post-embryonic pathways.
Article
Plant Sciences
Sofija Stupar, Milan Dragicevic, Vele Tesevic, Jovana Stankovic-Jeremic, Vuk Maksimovic, Tatjana Cosic, Nina Devrnja, Ljiljana Tubic, Aleksandar Cingel, Branka Vinterhalter, Slavica Ninkovic, Jelena Savic
Summary: A microarray transcriptome analysis was conducted to study the effects of French Marigold essential oil on potato metabolism, revealing that exposure to the oil suppressed genes related to photosynthesis and altered leaf starch metabolism. Additionally, the exposure led to an increase in sucrose accumulation in potato plants compared to glucose and fructose. The changes in transcriptional levels and sugar metabolism caused by the essential oil also resulted in enhanced feeding behavior and development of Colorado potato beetle larvae.
Review
Biochemistry & Molecular Biology
Martin Raspor, Vaclav Motyka, Abdul Rasheed Kaleri, Slavica Ninkovic, Ljiljana Tubic, Aleksandar Cingel, Tatjana Cosic
Summary: De novo shoot organogenesis (DNSO) is a common procedure used for shoot regeneration in vitro. The genetic regulation of DNSO has been studied in detail, highlighting the roles of plant hormones cytokinin (CK) and auxin, as well as their interaction with sucrose in the regeneration media. These signaling events mediate the complex process of shoot regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Dusica Calic, Jelena Milojevic, Maja Belic, Rade Miletic, Snezana Zdravkovic-Korac
Summary: This study found that the viability and germination rate of fresh pollen varied under different storage temperatures, with Budimka cultivar showing the highest viability and germination rate. Storage at 4 degrees Celsius extended the pollen viability and germinability, while -20 or -80 degrees Celsius significantly prolonged the pollen longevity for all cultivars. After 6 months, pollen stored at -20 and -80 degrees Celsius had much higher germination rates compared to pollen stored at room temperature for the same period.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Dobrivoj Postic, Ratibor Strbanovic, Marijenka Tabakovic, Tatjana Popovic, Ana Ciric, Nevena Banjac, Nenad Trkulja, Rade Stanisavljevic
Summary: Seed treatments with zinc, boron, biostimulant Coveron and MIX have shown positive effects on seed germination and seedling growth of various lettuce, celeriac, and wheat cultivars, while also inhibiting the growth of pathogens. Different cultivars showed varying responses to the seed treatments, with significant interactions observed between cultivars and treatments. The relationship between seed germination and seedling growth was found to be significant, while the relationship between seed germination and pathogens was negative for all cultivars.
Article
Plant Sciences
Snezana Zdravkovic-Korac, Jelena Milojevic, Maja Belic, Dusica Calic
Summary: Species of the genus Aesculus, such as horse chestnut, have medicinal uses and can be used for the treatment of venous insufficiency. Various in vitro propagation methods have been established for mass production of embryos and shoots, including somatic embryogenesis and shoot organogenesis. Additionally, an efficient method for cryopreservation of embryogenic tissue has been developed to ensure constant availability of the plant material.
Article
Plant Sciences
Nina Devrnja, Uros Gasic, Sanja Sajkunic, Aleksandar Cingel, Sofija Stupar, Ljiljana Tubic, Jelena Savic
Summary: French marigold, a plant rich in polyphenolic secondary metabolites, was examined for its pesticidal potential. The methanol extract of the flower showed high antioxidant activity and demonstrated promising antifeedant effects against Colorado potato beetle larvae.
Article
Plant Sciences
Dragan Vinterhalter, Branka Vinterhalter, Vaclav Motyka
Summary: This study found that hypocotyl elongation of sunflower seedlings varied under different light conditions, with rhythmic elongation under diurnal photoperiods and uniform elongation under continuous light or darkness.
Article
Agronomy
Igor Kostic, Slobodan Milanovic, Miroslav Kostic, Darka Seslija Jovanovic, Dusica Calic, Libor Jankovsky, Jelica Lazarevic
Summary: The study explored the potential use of two EO compounds, trans-anethole and S-(+)-carvone, against gypsy moth larvae, showing that anethole and carvone may be used as control agents against GML, affecting their feeding and growth to a certain extent.
Article
Plant Sciences
Jelica Lazarevic, Igor Kostic, Darka Seslija Jovanovic, Dusica Calic, Slobodan Milanovic, Miroslav Kostic
Summary: The antifeedant activity of pure camphor and a thujone-camphor mixture against Colorado potato beetle was examined. The compounds showed efficacy in reducing leaf damage, but their effectiveness varied depending on the developmental stage of the beetle and the concentration applied. Promising eco-friendly antifeedants were identified.
Article
Horticulture
Nevena Banjac, Dijana Krstic-Milosevic, Tatjana Mijalkovic, Mirjana Petrovic, Tatjana Cosic, Mariana Stanisic, Branka Vinterhalter
Summary: In this study, an efficient tissue culture system for improving the rocket cultivar Domaca rukola was established. Optimal shoot multiplication and organogenesis protocols were determined through experiments with different hormone concentrations and combinations. In addition, in vitro-produced rocket shoots were found to be a potential source of the antioxidant flavonoid kaempferol.
Article
Plant Sciences
Sofija Stupar, Vaclav Motyka, Petre I. Dobrev, Tatjana Cosic, Nina Devrnja, Ljiljana Tubic, Jelena Savic
Summary: The study investigated the comprehensive phytohormone profile of potato plants exposed to French marigold essential oil (EO) for different time periods. The results showed altered phytohormone responses of EO-exposed potato plants, with stress-related phytohormones being the most affected. The expression levels of genes involved in biosynthesis and catabolism of stress-related phytohormones indicated that the jasmonic acid biosynthetic pathway was the most affected.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biology
Panagiota Kolitsida, Vladimir Nolic, Jianwen Zhou, Michael Stumpe, Natalie M. Niemi, Jorn Dengjel, Hagai Abeliovich
Summary: The mitophagic degradation of mitochondrial matrix proteins in Saccharomyces cerevisiae is selectively regulated by the matrix kinases Pkp1 and Pkp2, which are in turn regulated by the phosphatase Aup1/Ptc6. These proteins also regulate the phosphorylation status and catalytic activity of the pyruvate dehydrogenase complex, which is critical for mitochondrial metabolism. The pyruvate dehydrogenase complex has been found to have a novel function in regulating mitophagic selectivity independent of its enzymatic activity.
LIFE SCIENCE ALLIANCE
(2023)
Review
Horticulture
Snezana Zdravkovic-Korac, Maja Belic, Dusica Calic, Jelena Milojevic
Summary: A spinach-supplemented diet offers various health benefits, but caution is necessary due to the potential health issues from high levels of oxalic acid and nitrate. Breeding can increase the levels of vitamins and phytochemicals, but conventional breeding is constrained by complex sex determination. By utilizing tissue culture techniques and biotechnological methods, limitations in spinach somatic embryogenesis can be overcome, providing a pathway for further research and improvement in the efficiency of this process.
Article
Agronomy
Dusica Calic, Jasna L. Ristic-Djurovic, Sasa Cirkovic, Jelena Milojevic, Maja Belic, Mariana Stanisic, Snezana Zdravkovic-Korac
Summary: This study demonstrates that exposing flax seeds to static magnetic fields can improve their germination and quality. Specifically, a 350 mT magnetic field treatment for 100 and 120 minutes achieved the best germination results and showed significant improvements in several biological indicators.