Control limits for accumulation of plant metabolites: brute force is no substitute for understanding

Engineering ascorbic acid biosynthetic pathway in Arabidopsis leaves by single and double gene transformation

(2012) Y. Zhou et al.   BIOLOGIA PLANTARUM

A comparative transcriptomic, fluxomic and metabolomic analysis of the response of Saccharomyces cerevisiae to increases in NADPH oxidation

(2012) Magalie Celton et al.   BMC GENOMICS

Retention during Processing and Bioaccessibility of β-Carotene in High β-Carotene Transgenic Cassava Root

(2012) Mark L. Failla et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants

(2012) N. Ruiz-Lopez et al.   JOURNAL OF EXPERIMENTAL BOTANY

The multifaceted role of aspartate-family amino acids in plant metabolism

(2012) M. Kirma et al.   JOURNAL OF EXPERIMENTAL BOTANY

Expression of a heterologous SnRK1 in tomato increases carbon assimilation, nitrogen uptake and modifies fruit development

(2012) Xinliang Wang et al.   JOURNAL OF PLANT PHYSIOLOGY

Expression of Arabidopsis plastidial phosphoglucomutase in tobacco stimulates photosynthetic carbon flow into starch synthesis

(2012) Kimio Uematsu et al.   JOURNAL OF PLANT PHYSIOLOGY

A constraint-based model analysis of the metabolic consequences of increased NADPH oxidation in Saccharomyces cerevisiae

(2012) Magalie Celton et al.   METABOLIC ENGINEERING

Confronting the Gordian knot

(2012) L Val Giddings et al.   NATURE BIOTECHNOLOGY

Post-Translational Redox Modification of ADP-Glucose Pyrophosphorylase in Response to Light is Not a Major Determinant of Fine Regulation of Transitory Starch Accumulation in Arabidopsis Leaves

(2012) Jun Li et al.   PLANT AND CELL PHYSIOLOGY

Engineering starch accumulation by manipulation of phosphate metabolism of starch

(2012) Sean E. Weise et al.   PLANT BIOTECHNOLOGY JOURNAL

Field trials and tribulations-making sense of the regulations for experimental field trials of transgenic crops in Europe

(2012) Sonia Gómez-Galera et al.   PLANT BIOTECHNOLOGY JOURNAL

Enhanced polyhydroxybutyrate production in transgenic sugarcane

(2012) Lars A. Petrasovits et al.   PLANT BIOTECHNOLOGY JOURNAL

Vitamin Deficiencies in Humans: Can Plant Science Help?

(2012) T. B. Fitzpatrick et al.   PLANT CELL

Ectopic overexpression of peach GDP-d-mannose pyrophosphorylase and GDP-d-mannose-3′,5′-epimerase in transgenic tobacco

(2012) Tsuyoshi Imai et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Recruiting a New Substrate for Triacylglycerol Synthesis in Plants: The Monoacylglycerol Acyltransferase Pathway

(2012) James R. Petrie et al.   PLoS One

Transgenic rice grains expressing a heterologous ρ-hydroxyphenylpyruvate dioxygenase shift tocopherol synthesis from the γ to the α isoform without increasing absolute tocopherol levels

(2012) Gemma Farré et al.   TRANSGENIC RESEARCH

Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing

(2012) Noemi Ruiz-López et al.   TRANSGENIC RESEARCH

Maize Provitamin A Carotenoids, Current Resources, and Future Metabolic Engineering Challenges

(2012) Eleanore T. Wurtzel et al.   Frontiers in Plant Science

The BioCassava Plus Program: Biofortification of Cassava for Sub-Saharan Africa

(2011) Richard Sayre et al.   Annual Review of Plant Biology

Folate Biosynthesis, Turnover, and Transport in Plants

(2011) Andrew D. Hanson et al.   Annual Review of Plant Biology

Response of Pseudomonas putida KT2440 to Increased NADH and ATP Demand

(2011) Birgitta E. Ebert et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacterial growth rate reflects a bottleneck in resource allocation

(2011) A. Goelzer et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Transgenic potato overproducing l-ascorbic acid resisted an increase in methylglyoxal under salinity stress via maintaining higher reduced glutathione level and glyoxalase enzyme activity

(2011) Chandrama Prakash Upadhyaya et al.   BIOTECHNOLOGY LETTERS

Microbial sucrose isomerases: Producing organisms, genes and enzymes

(2011) Ken C. Goulter et al.   ENZYME AND MICROBIAL TECHNOLOGY

A golden era—pro-vitamin A enhancement in diverse crops

(2011) Chao Bai et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Compartmentation of Triacylglycerol Accumulation in Plants

(2011) Kent D. Chapman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

High levan accumulation in transgenic tobacco plants expressing the Gluconacetobacter diazotrophicus levansucrase gene

(2011) Alexander Banguela et al.   JOURNAL OF BIOTECHNOLOGY

Use of TILLING and robotised enzyme assays to generate an allelic series of Arabidopsis thaliana mutants with altered ADP-glucose pyrophosphorylase activity

(2011) Nadja Hädrich et al.   JOURNAL OF PLANT PHYSIOLOGY

An in vivo data-driven framework for classification and quantification of enzyme kinetics and determination of apparent thermodynamic data

(2011) André B. Canelas et al.   METABOLIC ENGINEERING

Remodeling the isoprenoid pathway in tobacco by expressing the cytoplasmic mevalonate pathway in chloroplasts

(2011) Shashi Kumar et al.   METABOLIC ENGINEERING

Membrane-Transport Systems for Sucrose in Relation to Whole-Plant Carbon Partitioning

(2011) Brian G. Ayre   Molecular Plant

Evidence against sink limitation by the sucrose-to-starch route in potato plants expressing fructosyltransferases

(2011) Ellen Zuther et al.   PHYSIOLOGIA PLANTARUM

Down-regulation of β-carotene hydroxylase increases β-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato

(2011) Sun Ha Kim et al.   PHYTOCHEMISTRY

Endoplasmic Reticulum-Located PDAT1-2 from Castor Bean Enhances Hydroxy Fatty Acid Accumulation in Transgenic Plants

(2011) Hyun Uk Kim et al.   PLANT AND CELL PHYSIOLOGY

Pathway engineering of plants toward astaxanthin production

(2011) Norihiko Misawa   Plant Biotechnology

Field performance of transgenic sugarcane expressing isomaltulose synthase

(2011) Shiromani W. V. Basnayake et al.   PLANT BIOTECHNOLOGY JOURNAL

Trade-offs between biomass growth and inducible biosynthesis of polyhydroxybutyrate in transgenic poplar

(2011) David A. Dalton et al.   PLANT BIOTECHNOLOGY JOURNAL

Peroxisomal polyhydroxyalkanoate biosynthesis is a promising strategy for bioplastic production in high biomass crops

(2011) Kimberley Tilbrook et al.   PLANT BIOTECHNOLOGY JOURNAL

The role of Δ6-desaturase acyl-carrier specificity in the efficient synthesis of long-chain polyunsaturated fatty acids in transgenic plants

(2011) Olga Sayanova et al.   PLANT BIOTECHNOLOGY JOURNAL

Enhancing ascorbate in fruits and tubers through over-expression of the l-galactose pathway gene GDP-l-galactose phosphorylase

(2011) Sean Bulley et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering plastid fatty acid biosynthesis to improve food quality and biofuel production in higher plants

(2011) Marcelo Rogalski et al.   PLANT BIOTECHNOLOGY JOURNAL

Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis

(2011)   Sanjaya et al.   PLANT BIOTECHNOLOGY JOURNAL

Isomaltulose Is Actively Metabolized in Plant Cells

(2011) L. Wu et al.   PLANT PHYSIOLOGY

High Levels of Bioplastic Are Produced in Fertile Transplastomic Tobacco Plants Engineered with a Synthetic Operon for the Production of Polyhydroxybutyrate

(2011) K. Bohmert-Tatarev et al.   PLANT PHYSIOLOGY

Nonsymbiotic Hemoglobin-2 Leads to an Elevated Energy State and to a Combined Increase in Polyunsaturated Fatty Acids and Total Oil Content When Overexpressed in Developing Seeds of Transgenic Arabidopsis Plants

(2011) H. Vigeolas et al.   PLANT PHYSIOLOGY

Altering Trehalose-6-Phosphate Content in Transgenic Potato Tubers Affects Tuber Growth and Alters Responsiveness to Hormones during Sprouting

(2011) S. Debast et al.   PLANT PHYSIOLOGY

Duplicate Maize Wrinkled1 Transcription Factors Activate Target Genes Involved in Seed Oil Biosynthesis

(2011) B. Pouvreau et al.   PLANT PHYSIOLOGY

Regulation of Starch Biosynthesis in Response to a Fluctuating Environment

(2011) P. Geigenberger   PLANT PHYSIOLOGY

Expression of Fungal diacylglycerol acyltransferase2 Genes to Increase Kernel Oil in Maize

(2011) J. Oakes et al.   PLANT PHYSIOLOGY

Controlling lipid accumulation in cereal grains

(2011) Guillaume Barthole et al.   PLANT SCIENCE

Manipulation of l-ascorbic acid biosynthesis pathways in Solanum lycopersicum: elevated GDP-mannose pyrophosphorylase activity enhances l-ascorbate levels in red fruit

(2011) Christelle Cronje et al.   PLANTA

Expression of polyhydroxybutyric acid as a model for metabolic engineering of soybean seed coats

(2011) J. A. Schnell et al.   TRANSGENIC RESEARCH

Nutritious crops producing multiple carotenoids – a metabolic balancing act

(2011) Gemma Farré et al.   TRENDS IN PLANT SCIENCE

Synthesis of Short-Chain-Length/Medium-Chain Length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisomes of Transgenic Sugarcane Plants

(2011) David J. Anderson et al.   Tropical Plant Biology

Level of ascorbic acid in transgenic rice for l-galactono-1,4-lactone dehydrogenase overexpressing or suppressed is associated with plant growth and seed set

(2010) Yonghai Liu et al.   ACTA PHYSIOLOGIAE PLANTARUM

Methylglyoxal, glyoxalase 1 and the dicarbonyl proteome

(2010) Naila Rabbani et al.   AMINO ACIDS

High-lysine maize: the key discoveries that have made it possible

(2010) R. A. Azevedo et al.   AMINO ACIDS

New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications

(2010) Chaofu Lu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes

(2010) Ken'ichiro Matsumoto et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative Stress Tolerance inArabidopsis

(2010) Zinan Wang et al.   Journal of Integrative Plant Biology

Kinetic and Thermodynamic Aspects of Enzyme Control and Regulation†

(2010) Johann M. Rohwer et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Ascorbic acid contents in transgenic potato plants overexpressing two dehydroascorbate reductase genes

(2010) Aiguo Qin et al.   MOLECULAR BIOLOGY REPORTS

Regulation must be revolutionized

(2010) Ingo Potrykus   NATURE

Rare genetic variation at Zea mays crtRB1 increases β-carotene in maize grain

(2010) Jianbing Yan et al.   NATURE GENETICS

Genetic engineering compared to natural genetic variations

(2010) Werner Arber   New Biotechnology

Genetically modified myths and realities

(2010) Wayne Parrott   New Biotechnology

Inactivation of allergens and toxins

(2010) Piero Morandini   New Biotechnology

The regulation of agricultural biotechnology: science shows a better way

(2010) Henry I. Miller   New Biotechnology

Golden Rice and ‘Golden’ crops for human nutrition

(2010) Peter Beyer   New Biotechnology

Co-ordinated synthesis of gentiobiitol and sorbitol, evidence of sorbitol glycosylation in transgenic sugarcane

(2010) Barrie Fong Chong et al.   PHYTOCHEMISTRY

The role of plastidial glucose-6-phosphate/phosphate translocators in vegetative tissues of Arabidopsis thaliana mutants impaired in starch biosynthesis

(2010) H. H. Kunz et al.   PLANT BIOLOGY

Synergistic metabolism in hybrid corn indicates bottlenecks in the carotenoid pathway and leads to the accumulation of extraordinary levels of the nutritionally important carotenoid zeaxanthin

(2010) Shaista Naqvi et al.   PLANT BIOTECHNOLOGY JOURNAL

Transgenic expression of trehalulose synthase results in high concentrations of the sucrose isomer trehalulose in mature stems of field-grown sugarcane

(2010) Dénes Hamerli et al.   PLANT BIOTECHNOLOGY JOURNAL

VernoniaDGATs increase accumulation of epoxy fatty acids in oil

(2010) Runzhi Li et al.   PLANT BIOTECHNOLOGY JOURNAL

Overexpression of SlGMEs leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato

(2010) Chanjuan Zhang et al.   PLANT CELL REPORTS

Coexpression Analysis Identifies Rice Starch Regulator1, a Rice AP2/EREBP Family Transcription Factor, as a Novel Rice Starch Biosynthesis Regulator

(2010) F.-F. Fu et al.   PLANT PHYSIOLOGY

Metabolic Engineering of Seeds Can Achieve Levels of  -7 Fatty Acids Comparable with the Highest Levels Found in Natural Plant Sources

(2010) H. T. Nguyen et al.   PLANT PHYSIOLOGY

Expression of ZmLEC1 and ZmWRI1 Increases Seed Oil Production in Maize

(2010) B. Shen et al.   PLANT PHYSIOLOGY

Current perspectives on the regulation of whole-plant carbohydrate partitioning

(2010) Thomas L. Slewinski et al.   PLANT SCIENCE

Physiological and developmental regulation of seed oil production

(2010) Sébastien Baud et al.   PROGRESS IN LIPID RESEARCH

Nanometer Propagation of Millisecond Motions in V-Type Allostery

(2010) James M. Lipchock et al.   STRUCTURE

Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content

(2010) Shaista Naqvi et al.   TRANSGENIC RESEARCH

Phenology, growth and physiological adjustments of oil palm (Elaeis guineensis) to sink limitation induced by fruit pruning

(2009) S. Legros et al.   ANNALS OF BOTANY

The second green revolution? Production of plant-based biodegradable plastics

(2009) Brian P. Mooney   BIOCHEMICAL JOURNAL

Increasing the flow of carbon into seed oil

(2009) Randall J. Weselake et al.   BIOTECHNOLOGY ADVANCES

Enhanced ascorbic acid accumulation in transgenic potato confers tolerance to various abiotic stresses

(2009) Hemavathi et al.   BIOTECHNOLOGY LETTERS

Molecular modification of triacylglycerol accumulation by over-expression of DGAT1 to produce canola with increased seed oil content under field conditionsThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

(2009) David C. Taylor et al.   Botany

Removing allergens and reducing toxins from food crops

(2009) Maria Gallo et al.   CURRENT OPINION IN BIOTECHNOLOGY

Pathway engineering of Brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation

(2009) Masaki Fujisawa et al.   JOURNAL OF EXPERIMENTAL BOTANY

Analysis of polyhydroxybutyrate flux limitations by systematic genetic and metabolic perturbations

(2009) Keith E.J. Tyo et al.   METABOLIC ENGINEERING

Developmental and Feedforward Control of the Expression of Folate Biosynthesis Genes in Tomato Fruit

(2009) Jeffrey C. Waller et al.   Molecular Plant

Understanding the regulation of aspartate metabolism using a model based on measured kinetic parameters

(2009) Gilles Curien et al.   Molecular Systems Biology

Metabolomics-driven quantitative analysis of ammonia assimilation in E. coli

(2009) Jie Yuan et al.   Molecular Systems Biology

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

(2009) Bryson D Bennett et al.   Nature Chemical Biology

Increase in Ascorbate Content of Transgenic Tobacco Plants Overexpressing the Acerola (Malpighia glabra) Phosphomannomutase Gene

(2009) A. A. Badejo et al.   PLANT AND CELL PHYSIOLOGY

Molecular cloning and characterization of aKCSgene fromCardamine graecaand its heterologous expression inBrassicaoilseeds to engineer high nervonic acid oils for potential medical and industrial use

(2009) David C. Taylor et al.   PLANT BIOTECHNOLOGY JOURNAL

Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways

(2009) Steve P. Slocombe et al.   PLANT BIOTECHNOLOGY JOURNAL

Tobacco as a production platform for biofuel: overexpression ofArabidopsis DGATandLEC2genes increases accumulation and shifts the composition of lipids in green biomass

(2009) Vyacheslav Andrianov et al.   PLANT BIOTECHNOLOGY JOURNAL

Repression of Seed Maturation Genes by a Trihelix Transcriptional Repressor in Arabidopsis Seedlings

(2009) M.-J. Gao et al.   PLANT CELL

Expression of Rapeseed Microsomal Lysophosphatidic Acid Acyltransferase Isozymes Enhances Seed Oil Content in Arabidopsis

(2009) S. Maisonneuve et al.   PLANT PHYSIOLOGY

Biosynthesis, regulation and functions of tocochromanols in plants

(2009) Laurent Mène-Saffrané et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Rethinking metabolic control

(2009) Piero Morandini   PLANT SCIENCE

Acetyl-CoA—Life at the metabolic nexus

(2009) David J. Oliver et al.   PLANT SCIENCE

Carotenoid Crystal Formation in Arabidopsis and Carrot Roots Caused by Increased Phytoene Synthase Protein Levels

(2009) Dirk Maass et al.   PLoS One

Transgenic multivitamin corn through biofortification of endosperm with three vitamins representing three distinct metabolic pathways

(2009) S. Naqvi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

NTRC links built-in thioredoxin to light and sucrose in regulating starch synthesis in chloroplasts and amyloplasts

(2009) J. Michalska et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Folate biofortification of lettuce by expression of a codon optimized chicken GTP cyclohydrolase I gene

(2009) Aline C. S. Nunes et al.   TRANSGENIC RESEARCH

When more is better: multigene engineering in plants

(2009) Shaista Naqvi et al.   TRENDS IN PLANT SCIENCE

Application of Functional Genomics to Pathway Optimization for Increased Isoprenoid Production

(2008) L. Kizer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genetic engineering of carotenoid formation in tomato fruit and the potential application of systems and synthetic biology approaches

(2008) Paul D. Fraser et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Metabolic Engineering of ω3-Very Long Chain Polyunsaturated Fatty Acid Production by an Exclusively Acyl-CoA-dependent Pathway

(2008) Mareike Hoffmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Metabolic Control Analysis: A Tool for Designing Strategies to Manipulate Metabolic Pathways

(2008) Rafael Moreno-Sánchez et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Decreased expression of plastidial adenylate kinase in potato tubers results in an enhanced rate of respiration and a stimulation of starch synthesis that is attributable to post-translational redox-activation of ADP-glucose pyrophosphorylase

(2008) Sandra N. Oliver et al.   JOURNAL OF EXPERIMENTAL BOTANY

Generation of transgenic maize with enhanced provitamin A content

(2008) Maneesha Aluru et al.   JOURNAL OF EXPERIMENTAL BOTANY

A phenylalanine in DGAT is a key determinant of oil content and composition in maize

(2008) Peizhong Zheng et al.   NATURE GENETICS

Arabidopsis 3-hydroxy-3-methylglutaryl-CoA reductase is regulated at the post-translational level in response to alterations of the sphingolipid and the sterol biosynthetic pathways

(2008) Benjamín Nieto et al.   PHYTOCHEMISTRY

Overriding the co-limiting import of carbon and energy into tuber amyloplasts increases the starch content and yield of transgenic potato plants

(2008) Lizhi Zhang et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of polyhydroxybutyrate in switchgrass, a value-added co-product in an important lignocellulosic biomass crop

(2008) Maria N. Somleva et al.   PLANT BIOTECHNOLOGY JOURNAL

Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil

(2008) Julie Burgal et al.   PLANT BIOTECHNOLOGY JOURNAL

Transport and Sorting of the Solanum tuberosum Sucrose Transporter SUT1 Is Affected by Posttranslational Modification

(2008) U. Krugel et al.   PLANT CELL

The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production

(2008) Atsushi Ishihara et al.   PLANT JOURNAL

Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering

(2008) Tomohisa Hasunuma et al.   PLANT JOURNAL

Production of renewable polymers from crop plants

(2008) Jan B. van Beilen et al.   PLANT JOURNAL

Improving the Content of Essential Amino Acids in Crop Plants: Goals and Opportunities

(2008) S. Ufaz et al.   PLANT PHYSIOLOGY

Efficient silencing of reporter transgenes coupled to known functional promoters in sugarcane, a highly polyploid crop species

(2008) Stephen R. Mudge et al.   PLANTA

Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize

(2008) C. Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Natural Genetic Variation in Lycopene Epsilon Cyclase Tapped for Maize Biofortification

(2008) C. E. Harjes et al.   SCIENCE

Identifying and characterising regulatory metabolites with generalised supply–demand analysis

(2007) Johann M. Rohwer et al.   JOURNAL OF THEORETICAL BIOLOGY

Folate biofortification in food plants

(2007) Samir Bekaert et al.   TRENDS IN PLANT SCIENCE