Article
Agronomy
Ayumu Takatsuka, Tomohiko Kazama, Kinya Toriyama
Summary: The study obtained a Tadukan-type CMS line and a restorer line through backcrossing, determined the whole-genome sequences of the TAA mitochondria, and identified the orf312 gene specific to this genome, which showed differential expression patterns at different growth stages.
Article
Plant Sciences
Ayumu Takatsuka, Tomohiko Kazama, Shin-ichi Arimura, Kinya Toriyama
Summary: Cytoplasmic male sterility (CMS) is a trait that causes dysfunction in pollen or anthers, leading to the lack of seed setting. The orf312 gene has been identified as the causative gene for Tadukan-type CMS (TAA) in rice. Knocking out orf312 in TAA plants restored anther dehiscence and seed setting, confirming its role as a CMS-causative gene.
Article
Biochemistry & Molecular Biology
Na Xu, Hai Xu, Zhengjin Xu, Fengcheng Li, Quan Xu
Summary: The commercial japonica/GJ variety DHX2 displayed hybrid sterility when crossing with other GJ varieties, which was improved by knocking out extra copies of the Sc gene in DHX2. The genome of DHX2 may have undergone slight introgression from Basmati1, and both DHX2 and Basmati1 harbored three copies of Sc. Additionally, the introduction of GS3 and BADH2/fgr genes from Basmati1 conferred the slender and fragrance grain characteristics of DHX2, providing a novel strategy for hybrid breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Conghao Xu, Yifeng Xu, Zhengji Wang, Xiaoyu Zhang, Yuying Wu, Xinyan Lu, Hongwei Sun, Lei Wang, Qinglu Zhang, Qinghua Zhang, Xianghua Li, Jinghua Xiao, Xu Li, Mingfu Zhao, Yidan Ouyang, Xianbo Huang, Qifa Zhang
Summary: Male sterility in plants is important for germplasm innovation and hybrid crop production. In this study, a rare genetic resource for dominant genic male sterility in rice, called Sanming Dominant Genic Male Sterility, was characterized and the gene SDGMS was identified. The activation of SDGMS by the movement of a transposon leads to abnormal programmed cell death of tapetal cells and triggers transcription reprogramming of genes responsive to biotic stress, resulting in male sterility. This study demonstrates the importance of ectopic gene activation in phenotypic diversity and practical utility.
NATIONAL SCIENCE REVIEW
(2023)
Article
Biochemistry & Molecular Biology
Le Zhou, Yingzhe Wang, Xiaobo Xu, Dong Yan, Weijie Yu, Yifan Miao, Bo Xu
Summary: This study investigated the molecular mechanism of male sterility in alfalfa using bulked segregant analysis and bulked segregant RNA-seq. The results identified candidate genes associated with male sterility in alfalfa and revealed two common detected candidate genes. This research provides a theory basis for further understanding the molecular mechanism of male sterility in alfalfa and has significant implications for the genetic breeding of Medicago sativa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Huili Yang, Yadong Xue, Bing Li, Yanan Lin, Haochuan Li, Zhanyong Guo, Weihua Li, Zhiyuan Fu, Dong Ding, Jihua Tang
Summary: This study identified a chimeric mitochondrial gene (ATP6C) as the factor causing CMS in maize, and discovered its mechanism of action involving reduction in the quantity and activity of assembled F1Fo-ATP synthase, accumulation of excess protons in the mitochondrial inner membrane space, triggering ROS burst, premature programmed cell death of tapetal cells, and pollen abortion.
Article
Plant Sciences
Wei Yan, Shuting Yuan, Yazhou Zu, Zhenyi Chang, Yiqi Li, Zhufeng Chen, Gang Xie, Lei Chen, Changqing Lu, Xing Wang Deng, Chengwei Yang, Chunjue Xu, Xiaoyan Tang
Summary: Cold stress is a major limitation to rice production and distribution. However, the molecular mechanisms behind cold tolerance in rice remain unclear. This study reveals that ornithine d-aminotransferase (OsOAT) plays a role in cold tolerance during the vegetative and reproductive development of rice.
Article
Plant Sciences
Yue Jin, Xinyue Song, Huizhong Chang, Yueyue Zhao, Chenhao Cao, Xinbao Qiu, Jun Zhu, Ertao Wang, Zhongnan Yang, Nan Yu
Summary: Pollen protects male sperm and regulates anther development, allowing flowering plants to adapt to different terrestrial environments. The SLR1 gene in rice plays a crucial role in anther development, mainly expressed in tapetal cells and tetrads, and interacts with OsMS188 to regulate pollen wall formation.
Article
Agronomy
Huadong Wang, Qing Xiao, Chao Wei, Hui Chen, Xiaohan Chen, Cheng Dai, Jing Wen, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen, Bin Yi
Summary: By utilizing Brassica 60 K array, bulk segregant analysis, and map-based cloning, a mitochondria-localized pentatricopeptide repeat protein gene was identified and transferred into the hau CMS line, where it successfully restored fertility. The research showed that Rfh was highly expressed in flower buds, and the functional divergence between RFH and rfh may be attributed to 59 amino acid residue differences in the deduced protein sequences.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Agronomy
Naoyuki Katsura, Kanna Itoh, Hiroaki Matsuhira, Yosuke Kuroda, Tomohiko Kubo, Kazuyoshi Kitazaki
Summary: Cytoplasmic male sterility (CMS) is a mitochondrion-encoded trait that specifically affects anther development. This study compared the anther development in two sugar beet CMS lines and found that both lines exhibited degeneration of microspores and tapetum, leading to similar male sterile phenotypes. However, the specific developmental abnormalities differed between the two lines. Additionally, this study revealed that mitochondrial appearance in the root apical meristem was normal, indicating no association between mitochondrial activation and CMS expression.
Article
Plant Sciences
Gang Wei, Hongxia Yang, Jingwen Wu, Zixian Xiong, Nan Wang
Summary: A rice male sterile mutant, ms10, induced by EMS was reported in this study. The mutant showed significant male sterility phenotype in the reproductive growth stage, possibly due to abnormal pollen and decreased exine waxy layer of the anther. Genetic analysis revealed that the male sterility phenotype is controlled by a single recessive nuclear gene and mapped to a specific region on chromosome 4.
PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Yun Chen, Wenping Zhu, Shudan Shi, Lina Wu, Shuanglin Du, Liangshen Jin, Kuan Yang, Wenjia Zhao, Jiaxin Yang, Longbiao Guo, Zhongwei Wang, Yi Zhang
Summary: The study established an easy and efficient method using RNAi and OsMYB76R as a reporter to create and identify GMS, aiming to promote research on pollen development and heterosis utilization. The method demonstrated successful preparation of OsPTD1 GMS, saving time, reducing costs, and providing more accurate results compared to current methods.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Haksong Pak, Haoyi Wang, Yusin Kim, Unchol Song, Mengxin Tu, Dezhi Wu, Lixi Jiang
Summary: Male sterility is widely used in hybrid seed production in rice, but the use of genic male sterility is limited due to high labour costs. A new method was developed by editing the OsOPR7 gene in rice, resulting in successful generation of male-sterile lines in a field crop, laying the foundation for establishing a two-line system for rice hybrid production.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Plant Sciences
Jingyi You, Min Li, Hongwei Li, Yulin Bai, Xuan Zhu, Xiangjun Kong, Xiaoyan Chen, Ruiyang Zhou
Summary: This study investigated the DNA methylation patterns in cytoplasmic male sterility (CMS) lines of cotton using bisulfite sequencing technology and RNA-Seq. The results showed that CMS lines had more hypermethylated genes compared to near-isogenic lines, indicating a higher susceptibility to methylation. Furthermore, differentially expressed methylated genes were mainly involved in vital metabolic pathways, and there was a negative correlation between gene methylation and expression. Additionally, five key genes potentially associated with CMS were identified.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Yu Zhang, Jie Wang, Qiuhong Pu, Ying Yang, Yonggang Lv, Jiawu Zhou, Jing Li, Xianneng Deng, Min Wang, Dayun Tao
Summary: The study on intraspecific hybrid sterility in Asian cultivated rice reveals the origin, differentiation, and genetic aspects among subgroups. The identification of conserved and specific loci responsible for hybrid sterility between subgroups contributes to the divergence of Asian cultivated rice. Future research aims to characterize the genes for hybrid sterility and segregation distortion to unlock the mysterious relationship between hybrid sterility and Asian cultivated rice divergence.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Aaron Yap, Peter Kindgren, Catherine Colas des Francs-Small, Tomohiko Kazama, Sandra K. Tanz, Kinya Toriyama, Ian Small
Article
Plant Sciences
Keisuke Igarashi, Tomohiko Kazama, Kinya Toriyama
PLANT AND CELL PHYSIOLOGY
(2016)
Article
Plant Sciences
Tomohiko Kazama, Etsuko Itabashi, Shinya Fujii, Takahiro Nakamura, Kinya Toriyama
Article
Agronomy
Kinya Toriyama, Tomohiko Kazama
Article
Agronomy
Tetsuya Murakami, Tomohiko Kazama, Kinya Toriyama
Review
Biochemistry & Molecular Biology
Kenji Osabe, Takahiro Kawanabe, Taku Sasaki, Ryo Ishikawa, Keiichi Okazaki, Elizabeth S. Dennis, Tomohiko Kazama, Ryo Fujimoto
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2012)
Article
Biochemistry & Molecular Biology
Keiko Kobayashi, Masuyo Kawabata, Keizo Hisano, Tomohiko Kazama, Ken Matsuoka, Mamoru Sugita, Takahiro Nakamura
NUCLEIC ACIDS RESEARCH
(2012)
Article
Plant Sciences
Keisuke Igarashi, Tomohiko Kazama, Keiji Motomura, Kinya Toriyama
PLANT AND CELL PHYSIOLOGY
(2013)
Article
Plant Sciences
Masayuki Okazaki, Tomohiko Kazama, Hayato Murata, Keiji Motomura, Kinya Toriyama
PLANT AND CELL PHYSIOLOGY
(2013)
Article
Multidisciplinary Sciences
Tomohiko Kazama, Kinya Toriyama
Article
Agronomy
Tomohiko Kazama, Kinya Toriyama
Article
Agronomy
Shinya Fujii, Tomohiko Kazama, Yukihiro Ito, Soichi Kojima, Kinya Toriyama
Editorial Material
Plant Sciences
Tomohiko Kazama, Yusuke Yagi, Kinya Toriyama, Takahiro Nakamura
FRONTIERS IN PLANT SCIENCE
(2013)
Article
Plant Sciences
Shiho Omukai, Shin-ich Arimura, Kinya Toriyama, Tomohiko Kazama
Summary: In this study, orf352 gene was successfully knocked out from the mitochondrial genome of a CMS rice variety using mitoTALENs technology. The results showed that plants with a chimeric mitochondrial gene encoding amino acids 179-352 of ORF352 exhibited the same shrunken pollen grain phenotype, while plants lacking orf352 partially restored pollen development but failed to set seed.
Article
Plant Sciences
Tomohiko Kazama, Miki Okuno, Yuta Watari, Shungo Yanase, Chie Koizuka, Yu Tsuruta, Hajime Sugaya, Atsushi Toyoda, Takehiko Itoh, Nobuhiro Tsutsumi, Kinya Toriyama, Nobuya Koizuka, Shin-ichi Arimura