Article
Plant Sciences
Alma Costarelli, Sara Cannavo, Martina Cerri, Roberto Maria Pellegrino, Lara Reale, Francesco Paolocci, Stefania Pasqualini
Summary: Azolla species have been traditionally used as biofertilizers in rice paddies in South-east Asia due to their high growth and N-2 fixation rates. In recent years, there is a growing interest in the diverse applications of Azolla as a sustainable plant material, but their high levels of feed deterrent flavonoids have hindered their use as a sustainable protein source for animals.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sanja Radman, Igor Jerkovic
Summary: The analysis of volatile organic compounds (VOCs) in two brown algae from the Adriatic Sea revealed differences between fresh and air-dried samples, particularly in the percentages of fatty acids and their derivatives, terpenes, and aliphatic compounds.
Review
Biochemical Research Methods
Shane Fitzgerald, Linda Holland, Waqar Ahmed, Birgit Piechulla, Stephen J. Fowler, Aoife Morrin
Summary: This review examines the impact of infection on the production of volatile metabolites and the value of volatile biomarkers in non-invasive detection. By linking microbial studies to clinical investigations, the metabolic species and pathways leading to volatile signatures can be robustly elucidated.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Chemical
Jaroslaw Widelski, Konstantia Graikou, Christos Ganos, Krystyna Skalicka-Wozniak, Ioanna Chinou
Summary: The study investigated the essential oils of nine selected Apiaceae plants cultivated in Poland, identifying over 60 different metabolites using Gas Chromatography-Mass Spectrometry. The essential oils from S. devenyense, H. dulce, T. japonica and P. luxurians were reported for the first time. Among all species examined, H. dulce, S. devenyense and S. libanotis showed the strongest antimicrobial activity against Gram-positive bacteria strains.
Article
Food Science & Technology
Maria Filatova, Kamila Bechynska, Jana Hajslova, Michal Stupak
Summary: This study employed gas chromatography coupled to high-resolution mass spectrometry to evaluate the sensory properties of plum brandies. The non-target screening approach enabled the assessment of different plum brandies and the construction of chemometric models for authentication and classification.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Matthew T. Bingman, Josephine L. Hinkley, Colin P. P. Bradley II, Callie A. Cole
Summary: This study monitored the volatile organic compounds (VOCs) in cider production and revealed the influence of lees resting and dry hopping on cider aroma. The findings suggest that different hop varieties and processing steps significantly alter the identity and concentration of aroma-important VOCs in dry-hopped cider.
APPLIED SCIENCES-BASEL
(2022)
Article
Thermodynamics
Bijoy Biswas, Aishwarya Arun Kumar, Yashasvi Bisht, Bhavya B. Krishna, Jitendra Kumar, Thallada Bhaskar
Summary: The study investigated the effects of temperatures, reaction times, and different solvents on hydrothermal liquefaction of Azolla filiculoides. It was found that using methanol and ethanol solvents significantly increased the bio-oil yield, with valuable compounds detected in the bio-oils. The bio-residue decomposition into products varied with different solvents.
Article
Agriculture, Multidisciplinary
Cheng Jin-tao, Chen Hai-wen, Ding Xiao-chen, Shen Tai, Peng Zhao-wen, Kong Qiu-sheng, Huang Yuan, Bie Zhi-long
Summary: The application of CPPU in fruit production reduces the content of important esters in melon fruit, interferes with phenylalanine metabolism pathway, and affects the production of aromatic esters.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Yanyan Lu, Jianbo Wang, Gang Shen, Jiuling Liu, Hongwei Zhu, Junning Zhao, Shitang He
Summary: A novel analytical method using GC-SAW was developed for rapid determination of pharmacological volatiles of turmeric. Curcumene, beta-sesquiphellandrene, and ar-turmerone were confirmed as the main pharmacological volatiles of turmeric. Different categories of turmeric samples were identified based on their quality characteristics from different parts and origins.
Article
Chemistry, Analytical
Sophia A. Schreckenbach, Denina Simmons, Adam Ladak, Lauren Mullin, Derek C. G. Muir, Myrna J. Simpson, Karl J. Jobst
Summary: This study developed a suspect screening method based on industrial compounds with bioaccumulation potential. Results showed that SQDIA has a significantly lower false-positive rate compared to standard DIA. Through suspect screening and nontargeted screening, multiple pollutants were identified, with some compounds confirmed through standards and structural analysis.
ANALYTICAL CHEMISTRY
(2021)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Plant Sciences
Wenxuan Quan, Jing Jin, Chenyu Qian, Chaochan Li, Hongying Zhou
Summary: In this study, volatile compounds from four Rosa chinensis cultivars were analyzed using HS-SPME and GC x GC-QTOFMS. A total of 122 volatiles were identified, with alcohols, alkanes, and esters being the major chemical groups. Different cultivars exhibited distinct aroma characteristics, with RCG having floral and rose descriptors, RBR containing a high content of phenylethyl alcohol, and RF containing a high content of 3,5-dimethoxytoluene.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Applied
Araceli Rivera-Perez, Roberto Romero-Gonzalez, Antonia Garrido Frenich
Summary: This study applied GC-HRMS-based fingerprinting to determine the geographical traceability of thyme and assess its processing effects. The results showed that the geographical origin had a greater influence on thyme fingerprints than the processing effects. Chemometric methods were used to effectively discriminate the geographical origin of thyme and identify differential compounds.
Article
Plant Sciences
Salwa M. Abdel Rahman, Maher A. Kamel, Mennatallah A. Ali, Badriyah S. Alotaibi, Ohud Muslat Aharthy, Mustafa Shukry, Hala Mohamed Abd El-Bary
Summary: This study compared the phytochemical composition and various biological activities of Azolla caroliniana and Azolla filiculoides. The results showed that the methanol extracts of both species exhibited antimicrobial, antioxidant, anti-inflammatory, and cytotoxicity activities, but with some differences in their chemical profiles. The methanolic extracts of A. caroliniana displayed higher cytotoxicity against HepG2 cells in a dose-dependent manner.
Article
Multidisciplinary Sciences
Cara Olivier, Bianca Allen, Laneke Luies
Summary: This study optimized a urine preparation procedure for non-targeted metabolomics using GC-MS. The direct analysis method showed superior repeatability and higher metabolome coverage compared to the organic acid methods. The findings contribute to the advancement of urine metabolomics research.
SCIENTIFIC REPORTS
(2023)
Article
Agricultural Engineering
Helena Trindade, Carolina Ferro Rodrigues, Rogerio Tenreiro, Ana Tenreiro, Luis Gaspar Pedro
Summary: This study evaluated the chemical stability and gene expression levels in Thymus caespititius Brot. essential oil, investigating the correlation between seasonal essential oil variation and terpene synthase gene expression patterns. Different genes showed varying effects on essential oil composition, suggesting a need for further research to identify the factors controlling the final bouquet of essential oil composition.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Helena Santos, Catarina Augusto, Pedro Reis, Cecilia Rego, Ana Cristina Figueiredo, Ana Margarida Fortes
Summary: This study characterized the volatile profile of Trincadeira grapes and investigated its changes under infection with Botrytis cinerea. The results showed that infected grapes had higher contents of ester acetates and lower contents of fatty acid esters. The up-regulation of a gene involved in volatile metabolism was also observed. These changes may impact the grape quality and defense against B. cinerea.
Article
Biochemistry & Molecular Biology
Ana Ruas, Angelica Graca, Joana Marto, Lidia Goncalves, Ana Oliveira, Alexandra Nogueira da Silva, Madalena Pimentel, Artur Mendes Moura, Ana Teresa Serra, Ana Cristina Figueiredo, Helena M. Ribeiro
Summary: This study assessed the chemical composition, bioactivity, and sensory properties of Portuguese essential oils and hydrolates. Some of the studied oils and hydrolates showed antioxidant and antimicrobial activities, making them possible candidates for more sustainable and responsibly sourced ingredients.
Review
Plant Sciences
Claudia S. Tavares, Jose A. Gameiro, Luisa B. Roseiro, A. Cristina Figueiredo
Summary: An hydrolate is a distillate obtained after essential oil distillation, consisting of distilled water, essential oil droplets, and water-soluble components. This review provides a comprehensive overview of the volatile components of 362 hydrolates from 194 species, detailing the corresponding essential oil components. It also surveys the extraction and analysis methodologies, characteristics, traditional uses, and biological properties of hydrolates.
PHYTOCHEMISTRY REVIEWS
(2022)
Article
Chemistry, Medicinal
Sara El-Kharraf, Soukaina El-Guendouz, Farah Abdellah, El Mestafa El Hadrami, Alexandra M. Machado, Claudia S. Tavares, Ana Cristina Figueiredo, Maria Graca Miguel
Summary: The present study estimated the kinetic extraction and energy consumption of simultaneous hydro- and steam-distillation (SHSD) and SHSD assisted by carbon dioxide (SHSDACD) using a modeling approach. The results showed that SHSDACD provided higher yields of volatile oils with shorter extraction time and lower energy consumption. Different extraction methods also affected the chemical composition and biological activities of the volatile oils.
Article
Food Science & Technology
Alexandra M. Machado, Andreia Tomas, Paulo Russo-Almeida, Aida Duarte, Marilia Antunes, Miguel Vilas-Boas, M. Graca Miguel, A. Cristina Figueiredo
Summary: The quality and physicochemical parameters of Portuguese monofloral honeys were evaluated, including pollen analysis, microbiological safety, and characterization of physicochemical parameters. Significant differences were found among different honey types in terms of physicochemical parameters, suggesting that specific parameters can aid in identifying monofloral honeys.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agronomy
Paula S. Coelho, Joao M. Reis, Ana L. Pereira, Aliana Vairinhos, Violeta Lopes, Jose M. Leitao
Summary: One hundred accessions of Diplotaxis tenuifolia and Eruca spp. were screened for resistance to downy mildew. Cultivated rocket accessions showed a resistant response, while wild rocket accessions exhibited higher susceptibility in cotyledons. There was a significant correlation between disease index in cotyledons and leaves. Molecular markers analysis revealed genetic distance between Diplotaxis and Eruca, along with diverse interactions with the pathogen. Future studies will focus on evaluating different responses under greenhouse or field production and genome mapping of downy mildew resistance-related genetic features.
Article
Plant Sciences
Alexandra M. Machado, Violeta Lopes, Ana M. Barata, Orlanda Povoa, Noemia Farinha, A. Cristina Figueiredo
Summary: This study evaluated the essential oils (EOs) of Origanum vulgare subsp. virens (Hoffmanns. & Link) Ietsw. isolated from different regions in mainland Portugal. The EOs were analyzed for their composition and yield using hydrodistillation and gas chromatography-mass spectrometry. The results showed a considerable variability in the composition of the EOs, emphasizing the importance of avoiding wild plant collections for market-relevant crop production.
Article
Multidisciplinary Sciences
Joselia Monteiro, Paula Scotti-Campos, Isabel Pais, A. Cristina Figueiredo, Dulce Viegas, Fernando Reboredo
Summary: The evaluation of the elemental content of moringa leaves and flowers revealed that they are a good source of macro and micronutrients, as well as important polyunsaturated fatty acids. The flowers contain a significant amount of soluble sugars. The essential oils extracted from different parts of the moringa plant are mainly composed of alkanes and fatty acids. Further studies will determine if the consumption of moringa can address nutritional imbalances and prevent hypertension and diabetes.
Article
Biotechnology & Applied Microbiology
Ana L. L. Pereira, Francisco Carrapico
Summary: This passage mainly discusses the method for studying the cyanophycin content in the nitrogen-fixing heterocystous symbiotic cyanobacterium, Anabaena azollae. Three fluorochromes were used to observe the blue and yellow fluorescence emitted from the polar nodes and cytoplasmic cyanophycin granules of the heterocysts. It was found that aluminum trichloride, lead acetate, and Wilson citroboric solution could be used to detect cyanophycin.
BIOTECHNIC & HISTOCHEMISTRY
(2023)
Article
Plant Sciences
Ana Margarida Rodrigues, Ana Rita Mendes, Maria Filomena Caeiro, Ana Cristina Figueiredo, Lia Ascensao
Summary: This study aimed to characterize the secretory structures of the endemic aromatic Asteraceae plant Santolina impressa, analyze its essential oil from the aerial organs, and evaluate its antiviral activity against herpes simplex viruses HSV-1 and HSV-2. The study found that Santolina impressa secreted an oleoresin and a resin rich in flavonoids through biseriate glandular trichomes and secretory ducts. The essential oil of Santolina impressa mainly consisted of monoterpenes, with β-pinene and β-phellandrene as the major components. The essential oil exhibited dose-dependent inhibition of HSV-1 and HSV-2 infections in Vero cells.
Article
Biochemistry & Molecular Biology
Claudia Tavares, Carolina Ferro Rodrigues, Elsa Goncalves, Alexandra M. Machado, Luis Pedro, Jose Barroso, Anabela Mauricio, Nuno Franco, Delio Raimundo, Valerio Pita, Claudia Sanchez, Ana Cristina Figueiredo
Summary: This study evaluated the effect of cultural factors and conservation methods on the volatile profile of 'Gala' apples. The results showed that irrigation and nitrogen treatments had minimal impact on the volatile profile, while storage conditions significantly altered the ratio between compounds.
Article
Chemistry, Multidisciplinary
Ana Lima, Filipe Arruda, Alexandre Janeiro, Tania Rodrigues, Jose Baptista, Ana Cristina Figueiredo, Elisabete Lima
Summary: This study aimed to determine the yield and chemical composition of essential oils extracted from different parts of Azorean Cryptomeria japonica and investigate their antimicrobial and brine shrimp lethality effects. Results showed differences in yield and chemical composition of the essential oils from different parts, as well as different biological activities, especially in the cone samples.
APPLIED SCIENCES-BASEL
(2023)
Article
Plant Sciences
Alexandra M. Machado, Violeta Lopes, Ana Maria Barata, Orlanda Povoa, Noemia Farinha, Ana Cristina Figueiredo
Summary: This study evaluated the essential oils of two Apiaceae species, Coriandrum sativum and Foeniculum vulgare, commonly grown in Portugal. The essential oils were analyzed and revealed differences in chemical composition between the fruits and vegetative aerial parts of coriander, as well as differences in composition between different samples of fennel fruits. These findings highlight the importance of understanding the variability of these species' essential oils.
