Article
Microbiology
Tan Liu, Zhihui Chen, Yunhua Xiao, Mingmin Yuan, Chenkai Zhou, Gang Liu, Jun Fang, Bo Yang
Summary: Nitrogen limitation and starvation can increase the lipid content of microalgal cells but decrease biomass productivity, pigment concentration, and protein content. Three-day nitrogen starvation is a suitable strategy to produce lipid-rich biomass and causes changes in cell morphology. Saturated fatty acids dominate, making microalgae a potential feedstock for biodiesel production.
Article
Energy & Fuels
Farzaneh Noori, Saeid Vahdat
Summary: This study aimed to evaluate the effect of different environmental shocks on the nutrients concentration in Chlorella sp. The results showed that protein content decreased significantly in all treatments, while lipid content increased significantly in treatments of starvation and high pH. In conclusion, environmental shocks can induce biosynthesis of lipids and fatty acids in Chlorella sp.
BIOENERGY RESEARCH
(2023)
Article
Engineering, Chemical
Hao-Cheng Yu, Chyi-How Lay, Peer Mohamed Abdul, Jane-Yii Wu
Summary: This study optimized the cultivation conditions for a local microalgae species, Chlorella sp. G3H3-1-2, with high pH-resistant, high growth rate, and high lipid content. It was found that a pH range of 6.0 to 8.0 with glucose as the carbon source resulted in the highest specific growth rate of 1.22 day(-1) for Chlorella sp. G3H3-1-2. However, the highest total FAMEs content of 59% in the biomass of 1.75 g/L was obtained when using the combination of glucose and (NH4)2SO4 at a high pH value of 10.
Article
Biotechnology & Applied Microbiology
Liufu Wang, Di Ming, Yingying pan, Liqiu Shi, Wenzhi Wei
Summary: The main aim of this study is to investigate the effects of combined nitrogen limitation and hydrogen peroxide treatment on lipid accumulation in Chlorella sorokiniana. In addition, the relationships between reactive oxygen species (ROS), Ca2+ and lipogenic genes were analyzed to study the regulative networks involved in lipid accumulation. The results showed that lipid content and levels of endogenous ROS, Ca2+ increased under nitrogen limitation with H2O2 added, and ROS level could affect lipid accumulation through lipogenic gene expression. The study provides an effective strategy to improve lipid accumulation in C. sorokiniana and offers evidence of the involvement of ROS, Ca2+ and lipogenic genes in lipid biosynthesis in microalgae under combined treatment.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Eldrin Dlr Arguelles, Milagrosa R. Martinez-Goss
Summary: Lipid-accumulating microalgae, such as Chlorolobion sp. and Chlorella sp., showed increased lipid yield and decreased biomass production under nitrogen-starved culture conditions. The obtained algal biodiesel exhibited high concentrations of saturated and monounsaturated fatty acid methyl esters, making them suitable for biodiesel production. Additionally, the biodiesel properties of the two microalgae met the set specifications of biodiesel standards EN 14214 (European) and ASTM D6751 (American), indicating their potential as raw materials for biodiesel production with superior fuel quality.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Agricultural Engineering
Shiyan Zheng, Shanyi Chen, Shangyun Zou, Yiwen Yan, Guang Gao, Meilin He, Changhai Wang, Hui Chen, Qiang Wang
Summary: Chlorella sp. demonstrated high efficiency in removing various nutrients in Pyropia-processing wastewater (PPW) while accumulating higher biomass and exhibiting superior biodiesel characteristics.
BIORESOURCE TECHNOLOGY
(2021)
Article
Energy & Fuels
Junying Zhu, Baowen Guo, Xu Li, Lin Cheng, Xugeng Huang, Zhuanghong Mo, Baoning Zong, Junfeng Rong
Summary: Biological removal of NOx was achieved by mixotrophic cultivation of Chlorella sp. C2 using HNO3 as an intermediate, which provided a new strategy for indirect BioDeNO(x) and overcame drawbacks in traditional technologies. The biomass productivity and nitrogen consumption rate were higher compared to using NaNO3 as the nitrogen source. The use of HNO3 minimized sodium ion accumulation and the medium could be reused for water conservation.
Article
Engineering, Chemical
Tianyi Bao, Yuanyuan Shao, Haiping Zhang, Jesse Zhu
Summary: This study investigates the distribution of nitrogen in biocrude obtained from hydrothermal liquefaction of microalgae under different reaction conditions. Three main forms of nitrogen-containing compounds were detected in the biocrude, and a reaction map was proposed to describe the nitrogen pathway during the liquefaction process.
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Paul Castro-Cosio, Elizabeth Monreal-Escalante, Reyna Romero-Geraldo, Carlos Angulo
Summary: This review paper discusses studies focused on maximizing the production of high-valued bioproducts in Schizochytrium sp. through culture media optimization and genetic/metabolic engineering using a biorefinery approach for biopharmaceutical and recombinant protein production.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Plant Sciences
Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima
Summary: This study analyzed the impact of membrane lipid remodeling on inorganic phosphate uptake and discovered the crucial role of MpPAH in the growth of Marchantia polymorpha. The mutation of MpPAH affects plant growth by suppressing the synthesis of plastid glycolipids through the endoplasmic reticulum pathway.
Article
Biotechnology & Applied Microbiology
Mana Nakayasu, Momoka Amano, Tsuyoshi Tanaka, Ginga Shimakawa, Yusuke Matsuda
Summary: Highly oil-accumulative diatoms have potential as biomass for biofuel production. Understanding the relationship between oil accumulation and photosynthesis under fluctuating environmental conditions is crucial for high-yield diatom oil harvesting. In this study, the physiological responses of two diatom species (Fistulifera solaris and Mayamaea sp. JPCC CTDA0820) to nitrogen starvation were characterized, revealing distinct nitrogen requirements and photosynthetic responses. F. solaris exhibited growth retardation and suppressed photosynthetic activity, while Mayamaea sp. maintained normal growth and decreased Chl a amount per cells. These findings suggest two types of photosynthetic responses in oleaginous diatoms under nitrogen starvation.
MARINE BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Muhammad Amin, Pakamas Chetpattananondh, Chin-Kui Cheng, Syed Kamran Sami, Muhammad Najam Khan
Summary: Drying methods such as freeze drying, oven drying, and solar drying were compared for marine Chlorella sp., with freeze drying showing advantages in lipid content and chlorophyll extraction. The choice of drying method had an impact on the chemical properties of algal biomass, but not significantly.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Environmental
Laure Wetterwald, Antoine Leybros, Gatien Fleury, Florian Delrue, Alexandra Dimitriades-Lemaire, Paul Chambonniere, Audrey Hertz
Summary: This study investigates the effects of water content, operating pressure and temperature on supercritical CO2 extraction of lipids from Chlorella vulgaris microalgae. The highest extraction yield was obtained with freeze-dried microalgae at 40℃ and 200 bar. However, extracts obtained at 40℃ and 250 bar had the highest fatty acid content, while those obtained at 60℃ and 250 bar had the highest cetane number and oxidative stability. Water in the initial biomass had a negative impact on total extraction yield, but promoted the extraction of fatty acids, particularly monounsaturated ones.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Hong Li, Jun Tan, Yun Mu, Jianfeng Gao
Summary: Chlorella sp. TLD6B, a species with high lipid concentrations and salt tolerance, was able to accumulate lipid under salt stress conditions. High salt stress promoted lipid accumulation at 0.2M NaCl, but inhibited cell growth. Transcriptomic analysis revealed enhanced expression of genes related to lipid synthesis under salt stress, clarifying the relationship between salt tolerance and lipid accumulation in Chlorella sp. TLD6B.
Article
Energy & Fuels
Hanxiao Wang, Yunhan Tang, Haiyan Gao, Hao Gao, Quanyu Zhao, Jiawen Li, Fengxue Xin, Wenming Zhang, Min Jiang
Summary: A co-culture system composed of Chlorella sp. and Rhodotorula toruloides was established to improve microbial lipid production. The mixed culture showed higher biomass and lipid production due to the oxygen emission and pH regulation provided by Chlorella sp. Optimal conditions were determined to further enhance biomass and lipid content. Different temperatures in the mixed culture system resulted in changes in fatty acid composition. Molasses showed great potential as a substrate for industrial applications.
Article
Biochemistry & Molecular Biology
Pablo Calzadilla, Jiao Zhan, Pierre Setif, Claire Lemaire, Daniel Solymosi, Natalia Battchikova, Qiang Wang, Diana Kirilovsky
Article
Plant Sciences
Longlong Wang, Maria Carmen Rubio, Xian Xin, Baoli Zhang, Qiuling Fan, Qiang Wang, Guogui Ning, Manuel Becana, Deqiang Duanmu
Review
Agronomy
Hui Chen, Xinyang Wang, Qiang Wang
GLOBAL CHANGE BIOLOGY BIOENERGY
(2020)
Article
Biochemistry & Molecular Biology
Jing Zhang, Zechen Bai, Min Ouyang, Xiumei Xu, Haibo Xiong, Qiang Wang, Bernhard Grimm, Jean-David Rochaix, Lixin Zhang
Summary: Fe-S clusters are ancient and important for life processes. The biogenesis of Fe-S clusters involves iron acquisition, sulfur mobilization, and cluster formation. Two Arabidopsis DnaJ proteins, DJA6 and DJA5, play a crucial role in facilitating iron incorporation into Fe-S clusters during chloroplast Fe-S cluster biogenesis. Loss of these proteins leads to defects in chloroplast Fe-S protein accumulation and photosynthesis dysfunction. Evolutionary analysis shows that DJA6 and DJA5 are highly conserved in photosynthetic organisms and have a strong evolutionary relationship with SUFE1, SUFC, and SUFD.
Article
Biology
Hui Chen, Qiang Wang
Summary: Microalgal lipids are promising feedstocks for biofuel production, particularly triacylglycerol, but addressing fundamental biological questions such as lipid biosynthesis regulation is crucial for commercial use. Understanding of microalgal lipid biosynthesis and potential solutions to obstacles provide valuable guidance for future research and application of microalgae-based lipids.
BIOLOGICAL REVIEWS
(2021)
Article
Biochemical Research Methods
Qi Zhang, Shengchao Yu, Qiang Wang, Mingkun Yang, Feng Ge
Summary: A label-free quantitative proteomic strategy was used to investigate the nitrogen deficiency response of Anabaena 7120, revealing significant changes in protein abundance at different time points. The study also uncovered proteomic perturbation and regulation of carbon and nitrogen metabolism under nitrogen deficiency, with time-specific dysregulated proteins involved in heterocyst development. These results provide novel insights into the molecular mechanisms of nitrogen stress responses and heterocyst development in Anabaena 7120.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Plant Sciences
Xiang Li, Chunling Xue, Hui Chen, Huafeng Zhang, Qiang Wang
Summary: This study discovered a small antisense RNA gene (ThfR) that interacts with the thf1 gene, regulating photosynthesis under nitrogen starvation or high-light stress, and enhancing the growth ability of cyanobacteria under high-light stress.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Long-Sheng Zhao, Chun-Yang Li, Xiu-Lan Chen, Qiang Wang, Yu-Zhong Zhang, Lu-Ning Liu
Summary: Visualization of the photosynthetic complexes in the thylakoid membranes of a cyanobacterium reveals their native architecture and interactions, providing insights into the organization and adaptation of the photosynthetic apparatus under environmental stress. This study sheds light on the molecular mechanisms of efficient light harvesting and energy conversion.
Review
Agronomy
Zhongliang Sun, Hui Chen, Liqin Sun, Qiang Wang
Summary: Today's world is facing the challenges of reducing carbon dioxide emissions and addressing the energy crisis. Microalgae have emerged as a promising solution, as they can use solar energy to convert CO2 into valuable compounds and biofuels, while also providing environmental benefits. However, there are limitations to microalgae-based production, such as low productivity. Genetic and metabolic modifications have shown potential in improving productivity. This review discusses the latest developments in microalgae-based production and its strategic importance in solving the current energy crisis and environmental pollution problems.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2023)
Review
Biochemistry & Molecular Biology
Jinlu Hu, Dan Wang, Hui Chen, Qiang Wang
Summary: Even though sunlight energy far outweighs the energy required by human activities, genetic engineering is expected to allow for further increases in the photosynthesis and productivity of microalgae to meet rising food and feed demand. Understanding the mechanisms that control photosynthesis will enable researchers to identify targets for genetic engineering and increase biomass yield, offsetting the costs of cultivation systems and downstream biomass processing. This review highlights the major achievements in manipulating the fundamental mechanisms of microalgal photosynthesis and biomass production through genetic engineering, as well as promising approaches for future microalgal-based biotechnology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Green & Sustainable Science & Technology
Weixian Chen, Tianpei Li, Yangyi Ren, Jiaming Wang, Hui Chen, Qiang Wang
Summary: Hydrogen is widely used in various industries and is considered a clean and environmentally friendly secondary energy source. However, most hydrogen production currently relies on fossil fuels, leading to significant emissions. Biohydrogen production offers a greener alternative, but the current instability of the production system hinders its industrialization. This review examines the key issues in biohydrogen production and suggests that coupling hydrogen production with organic waste treatment and combining different strategies may be a viable approach for stable and scalable production.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Plant Sciences
Yanli Zheng, Chunling Xue, Hui Chen, Anqi Jia, Liang Zhao, Junli Zhang, Lixin Zhang, Qiang Wang
Summary: By utilizing synthetic biology, the biosynthetic gene cluster of MC-LR in Microcystis aeruginosa was reassembled and expressed in a model cyanobacterium, achieving autotrophic production of MC-LR. The study revealed that the expression of MC-LR caused abnormal cell division and filamentation, and further investigation demonstrated that MC-LR inhibits cell division by irreversibly competing for the GTP-binding site of the cell division protein FtsZ. This research lays the foundation for understanding the biosynthetic pathway of microcystins and reveals a mechanism of how blooming cyanobacteria gain a competitive edge.
Article
Agricultural Engineering
Shiyan Zheng, Aihua Wu, Hongyan Wang, Lei Chen, Jiamei Song, Huai Zhang, Meilin He, Changhai Wang, Hui Chen, Qiang Wang
Summary: In this study, the effects of temperature on nutrient removal efficiency of Pyropia-processing wastewater (PPW) and microalgal biomass production were investigated. The results showed that higher biomass and total lipids were attained at 35 degrees C. The maximal COD, phycobiliprotein, total nitrogen, and total phosphorus removal rates were observed at 30-35 degrees C. Additionally, chitosan assisted in achieving high COD removal efficiencies and clarification efficiency of algal cells.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Xiang Li, Wenwen Hou, Jiaxi Lei, Hui Chen, Qiang Wang
Summary: The phycobilisome is a protein complex found in cyanobacteria and red algae that serves as their main light-harvesting apparatus. It can absorb light between 450 and 650 nm due to the functional tuning of different phycobiliproteins mediated by linker proteins. Understanding the role of phycobilisomes in photosynthesis and exploring their potential applications requires basic research and technological innovations, particularly in synthetic biology. This review focuses on the essential components of phycobilisome assembly, the functional basis of its photosynthesis, and the applications of phycobiliproteins, as well as the challenges in heterologous biosynthesis of phycobiliproteins in chassis cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Environmental Sciences
Yunusa Adamu Ugya, Hui Chen, Yangyang Sheng, Fidelis Odedishemi Ajibade, Qiang Wang
Summary: This comprehensive review focuses on the challenges hindering the large-scale production of microalgae-based bioplastics, particularly economic feasibility and bioplastic quality. Microalgae biofilm cultivation is explored as a sustainable and highly viable approach for bioplastic production, and a proposed method is presented and evaluated for its environmental impact. The findings demonstrate that using microalgae biofilm for bioplastic production offers significant environmental sustainability benefits, with low energy requirements and a minimal carbon footprint.
ENVIRONMENTAL RESEARCH
(2023)