Article
Biochemistry & Molecular Biology
Viviana Scalavino, Emanuele Piccinno, Antonio Lacalamita, Angela Tafaro, Raffaele Armentano, Gianluigi Giannelli, Grazia Serino
Summary: This study identified 18 dysregulated miRNAs in intestinal epithelial cells from the ulcerative colitis (UC) mice model. MiR-195-5p, one of the down-regulated miRNAs, was found to be involved in impaired barrier function. The study demonstrated that miR-195-5p can regulate tight junctions by decreasing CLDN2 levels and subsequently affecting CLDN1 expression.
Article
Multidisciplinary Sciences
Kazuto Tsukita, Manabu Kitamata, Hiroka Kashihara, Tomoki Yano, Ikuko Fujiwara, Timothy F. Day, Tatsuya Katsuno, Jaewon Kim, Fumiko Takenaga, Hiroo Tanaka, Sungsu Park, Makoto Miyata, Hitomi Watanabe, Gen Kondoh, Ryosuke Takahashi, Atsushi Tamura, Sachiko Tsukita
Summary: Liquid-liquid phase separation (LLPS) plays a role in dynamic biological phenomena. In this study, the role of LLPS in the dynamic regulation of junctional actin filaments in epithelial cells was investigated. The researchers identified a multifunctional actin nucleator called cordon bleu (Cobl) as an AJC-enriched microtubule-associated protein and conducted comprehensive in vitro and in vivo analyses. They found that apical microtubules promoted LLPS of Cobl at the AJC, leading to increased Cobl actin assembly activity and spatiotemporal regulation of junctional actin assembly for epithelial morphogenesis and paracellular barriers.
Review
Food Science & Technology
Marta Justyna Koziel, Maksymilian Ziaja, Agnieszka Wanda Piastowska-Ciesielska
Summary: The intestinal barrier acts as the main defense against substances entering the body, ensuring balance in the organism; Mycotoxins, toxic fungi metabolites, may disrupt homeostasis by affecting the intestinal barrier; Claudins, proteins involved in tight junction formation between epithelial cells, play a significant role in responding to mycotoxin-induced cytotoxicity.
Article
Cell Biology
Shaista Anwer, Emily Branchard, Qinghong Dan, Angela Dan, Katalin Szaszi
Summary: Claudins are crucial for tight junction formation and paracellular transport. TNFa can impact epithelial permeability through modulating the expression of specific claudins, including claudin-3. The effect of TNFa on claudin-3 is mediated by ERK-dependent activation of NF-kappa B and PKA-induced activation of CREB1 pathways, both of which are necessary for the upregulation of claudin-3.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Medicine, General & Internal
Spyridoula Katsarou, Michael Makris, Efstratios Vakirlis, Stamatios Gregoriou
Summary: This systematic review focuses on the role of tight junctions in atopic dermatitis and their potential impact on therapeutic approaches. The findings suggest that dysfunctional tight junctions are closely associated with the pathogenesis and inflammation in atopic dermatitis, and further investigation into their functionality may lead to improved epidermal barrier function in this condition.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Nicolas Obtel, Adeline Le Cabec, The Nghia Nguyen, Eloise Giabicani, Stijn J. M. Van Malderen, Jan Garrevoet, Aline Percot, Celine Paris, Christopher Dean, Smail Hadj-Rabia, Pascal Houillier, Tilman Breiderhoff, Claire Bardet, Thibaud Coradin, Fernando Ramirez Rozzi, Catherine Chaussain
Summary: Claudin proteins are crucial for determining the permeability and ion specificity of tight junctions in epithelia. Deficiency in claudin-10 is associated with severe enamel wear in HELIX syndrome patients. Analysis of a HELIX patient's molar revealed accentuated strontium variations in the enamel and dentin, possibly indicating episodes of worsening renal dysfunction.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Endocrinology & Metabolism
Lars Winkler, Rosel Blasig, Olga Breitkreuz-Korff, Philipp Berndt, Sophie Dithmer, Hans C. Helms, Dmytro Puchkov, Kavi Devraj, Mehmet Kaya, Zhihai Qin, Stefan Liebner, Hartwig Wolburg, Anuska Andjelkovic, Andre Rex, Ingolf E. Blasig, Reiner F. Haseloff
Summary: The study shows that Cldn3 and occludin play a protective role in the stroke by maintaining the integrity of TJ and BBB, but also contribute to the development of edema and infarction.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Engineering, Biomedical
Elisa Boehm, Linda Droessler, Salah Amasheh
Summary: CBD, as a component of Cannabis-based biomaterials, has a strengthening effect on the cellular barrier under inflammatory conditions and mitigates the disruption of paracellular permeability and sealing proteins. These findings demonstrate the potential of CBD in therapeutic approaches against inflammatory pathogenesis.
MATERIALS TODAY BIO
(2023)
Article
Medicine, Research & Experimental
Lorena Rosas-Martinez, Rafael Rodriguez-Munoz, Maria del Carmen Namorado-Tonix, Fanis Missirlis, Leonardo del Valle-Mondragon, Alicia Sanchez-Mendoza, Jose L. Reyes-Sanchez, Luz Graciela Cervantes-Perez
Summary: This study aims to investigate the impact of hyperglycemia on renal expression of claudins-2 and -5 in diabetic nephropathy and the role of glucose-induced oxidative stress. The results show that chronic high-glycemia levels in early stages of diabetic nephropathy decrease expression of claudins-2 and -5, increase oxidative stress, and induce MMP-activity faster than chronic middle-glycemia levels. The level of hyperglycemia plays a crucial role in the progression of diabetic nephropathy.
Article
Food Science & Technology
Zheng Cao, Jinsong Gao, Wanyue Huang, Juli Yan, Anshan Shan, Xiang Gao
Summary: Curcumin protects against Deoxynivalenol-induced disruption of intestinal epithelial barrier by regulating apoptotic pathway and TJs pathway.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Alessandro Berselli, Giulio Alberini, Fabio Benfenati, Luca Maragliano
Summary: Claudins are a family of protein homologs that play a crucial role in cell-to-cell tight junctions. Despite experimental evidence suggesting that Claudin 4 can form anion-selective channels in the renal epithelium, its exact function remains unclear. This study uses computational methods to transfer structural models of Claudin-based complexes to Claudin 4 and investigates the thermodynamic properties driving ion selectivity. The results show that one model allows chloride permeability and repels cations, while the other forms a barrier to the passage of major physiological ions. Additionally, the study confirms the charge selectivity role of the residue Lys65 in regulating paracellular permeability.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Urology & Nephrology
Ali S. Alzahrani, Maged Hussein, Meshael Alswailem, Ahmad Mouna, Lina Albalawi, Yosra Moria, Mai Abdel Jabbar, Yufei Shi, Dorothee Guenzel, Majed Dasouki
Summary: A novel CLDN10 mutation was identified in two unrelated Saudi families with HELIX syndrome, disrupting the C-terminus pdz-binding motif of claudin-10. Functional studies indicated that this mutation led to mRNA degradation, protein retention in intracellular compartments, and translocation of other claudins in renal and sweat gland tissues.
KIDNEY INTERNATIONAL
(2021)
Review
Oncology
Abderrahman Ouban, Tarek Ziad Arabi
Summary: Premalignant lesions of the gastrointestinal tract are crucial in predicting and preventing the development of malignant tumors. Alterations in claudin protein expression have been found in various cancers, and their early identification can aid in the diagnosis and management of gastrointestinal tumors.
Review
Cell Biology
Chinemerem P. Ogbu, Sourav Roy, Alex J. Vecchio
Summary: Claudins are integral membrane proteins that play a crucial role in cell-cell interactions by forming tight junctions. The bacterium Clostridium perfringens causes gastrointestinal disorders by targeting claudins with its enterotoxin (CpE), disrupting the barrier function of the gut. Understanding the structure and function of Claudin and CpE provides insights into the mechanisms underlying the disruption of tight junctions. This review summarizes the current progress in this field and highlights the importance of structural studies in developing therapeutic strategies.
Article
Fisheries
Peng He, Lin Feng, Lu Zhang, Hai-Feng Mi, Pei Wu, Yang Liu, Jun Jiang, Sheng-Yao Kuang, Ling Tang, Xiao-Qiu Zhou, Wei-Dan Jiang
Summary: The study revealed that biotin deficiency significantly increased oxidative damage, aggravated cell apoptosis, and damaged tight junctions in immune organs, potentially through modulation of Nrf2, p38MAPK, and MLCK signaling pathways.
