Article
Agricultural Engineering
Xiangyu Guan, Biying Lin, Yuanmei Xu, Shunqin Bai, Rui Li, Shaojin Wang
Summary: This study aims to investigate the thermal inactivation kinetics of Acidovorax citrulli to design an effective control protocol for watermelon seeds. Results showed that the Weibull model was more suitable for studying the heat resistance of A. citrulli on embryos compared to a first-order kinetic model, and proposed a reliable prediction of population reduction of A. citrulli using thermal death kinetics.
BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Jie Jiang, Mei Zhao, Xiaoxiao Zhang, Linlin Yang, Nuoya Fei, Weiqin Ji, Wei Guan, Ron Walcott, Yuwen Yang, Tingchang Zhao
Summary: This study investigated bacterial fruit blotch caused by Acidovorax citrulli, and found that its secreted effector protein AopV suppresses plant immunity and interacts with the aromatic dehydratase ADT6 in watermelon. These findings provide new insights into the molecular interaction of AopV with host cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Anthikan Klomchit, Jorge Daniel Calderin, Wuttichai Jaidee, Kanchana Watla-iad, Siraprapa Brooks
Summary: The study identified Neocosmospora sp. MFLUCC 17-0253 as a potential biocontrol agent for bacterial fruit blotch (BFB) disease, showing promising antibacterial properties against the pathogen Acidovorax citrulli. The fungus produced multiple secondary metabolites that exhibited antagonistic activity against the causative agent of BFB, successfully colonizing the host plant and inhibiting biofilm development of Aac. The chemical profile data and biofilm assay suggest that Neocosmospora sp. MFLUCC 17-0253 has high potential for controlling BFB disease.
Article
Microbiology
Linlin Yang, Mei Zhao, Xiaoxiao Zhang, Jie Jiang, Nuoya Fei, Weiqin Ji, Yunfeng Ye, Wei Guan, Yuwen Yang, Tingchang Zhao
Summary: This study provides new insights into the mechanism of plant immune responses regulated by T3Es in A. citrulli. A novel T3E called AopU was identified and shown to affect host immunity, sharing homology with XopU in Xanthomonas oryzae.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shoko Fujiwara, Mana Toshio, Eri Nakayama, Naotaka Tanaka, Mitsuaki Tabuchi
Summary: The study reveals that Aave_4606 recognizes and activates specific host Trxs to decrease the host cellular GSH, thereby promoting the pathogenic bacteria growth.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Min Young Chung, Hoikyung Kim, Larry R. Beuchat, Jee-Hoon Ryu
Summary: The study developed a seed decontamination treatment for organic seeds against plant pathogens using essential oil vapours while maintaining germination rates. The applications of EO vapours showed significant antimicrobial effects against the pathogens without affecting seed germination rates.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Sumer Horuz
Summary: This study showed that certain bacterial biocontrol agents from the phyllosphere of melon and watermelon can significantly reduce disease incidence, providing useful solutions for disease control in cucurbit production. Further field studies are planned to validate these findings.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Ana Maria Hernandez-Anguiano, Vicente Rosas-Medina, Cristian Nava-Diaz, Jose Gustavo Torres-Martinez
Summary: In Mexico, the presence of Acidovorax citrulli, the causal agent of bacterial fruit blotch and seedling blight in cucurbits, has not been reported. However, there is a latent risk of its introduction through the importation of seeds and seedlings. This study detected A. citrulli in watermelon seedlings in Hopelchen, Campeche, using PCR analysis, confirming the presence of the bacterium and its potential spread to other cucurbit-producing areas in Mexico.
Review
Agronomy
Ainur Ainiah Azman Husni, Siti Izera Ismail, Noraini Jaafar, Dzarifah Zulperi
Summary: Bacterial fruit blotch (BFB) caused by Acidovorax citrulli is a devastating disease for cucurbits, especially watermelon- and melon producing regions, leading to massive yield losses globally. Recent studies have identified two distinct groups of A. citrulli strains based on their aggressiveness and host preferences, providing insights for more efficient BFB control strategies.
PLANT PROTECTION SCIENCE
(2021)
Article
Agronomy
Emanuel Feitosa de Assuncao, Claudeana Souza da Conceicao, Elizabeth Rodrigues Alexandre, Marco Aurelio Siqueira da Gama, Glauber Henrique de Souza Nunes, Elineide Barbosa de Souza
Summary: The study aims to identify melon accessions with genetic resistance to bacterial fruit blotch, with accessions I-136 and A-43 showing high levels of resistance in multiple assays. The results suggest that these accessions can be potential donors of resistance genes for genetic improvement programs in commercial cultivars.
Article
Immunology
Shiho Fukushima, Takaaki Shimohata, Yuri Inoue, Junko Kido, Takashi Uebanso, Kazuaki Mawatari, Akira Takahashi
Summary: Campylobacter jejuni infection activates the Rac1 signaling pathway to promote bacterial internalization, and LC3 plays a role in regulating the invasion signaling of C. jejuni, thereby participating in autophagy-mediated host immune response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Plant Sciences
Edgar H. Nieto-Lopez, Daniel G. Cerritos-Garcia, Rachel A. Koch A. Bach, Aparna Petkar, Christine D. Smart, Christy Hoepting, David Langston, Steven Rideout, Bhabesh Dutta, Sydney E. Everhart
Summary: Alternaria leaf blight and head rot is a serious disease in broccoli and other cole crops in the southeastern US. The use of QoI fungicides has been ineffective in controlling the disease. Two species of Alternaria, A. brassicicola and A. japonica, were isolated from symptomatic lesions in different states. A. japonica showed higher resistance to azoxystrobin, which may contribute to the loss of disease control.
Article
Plant Sciences
Christian Silva, Jaclyn A. Adaskaveg, Saskia D. Mesquida-Pesci, Isabel B. Ortega-Salazar, Sivakumar Pattathil, Lisha Zhang, Michael G. Hahn, Jan A. L. van Kan, Dario Cantu, Ann L. T. Powell, Barbara Blanco-Ulate
Summary: Postharvest fungal pathogen Botrytis cinerea actively induces ripening in tomato fruit to facilitate infection, depending on the presence of two fungal polygalacturonases. It accelerates external coloration, ethylene production, and softening in unripe fruit while upregulating host genes involved in ripening processes. Glycomic analysis also showed similar changes in cell wall polysaccharides between infected unripe fruit and ripe healthy fruit. The failure to accelerate ripening severely inhibits fungal survival on unripe fruit.
Article
Biochemistry & Molecular Biology
Mei Zhao, Gi Yoon Shin, Shaun Stice, Jonathon Luke Bown, Teresa Coutinho, William W. Metcalf, Ron Gitaitis, Brian Kvitko, Bhabesh Dutta
Summary: This study reports onion pathogenicity determinants in Pantoea stewartii subsp. indologenes and P. allii. Two distinct secondary metabolite biosynthetic gene clusters were identified in these species, similar to the HiVir phosphonate biosynthetic cluster in P. ananatis and a novel putative phosphonate biosynthetic gene cluster called Halophos. Deletion of the pepM gene from either cluster caused loss of pathogenicity, indicating the importance of these clusters in onion necrosis. Furthermore, exogenous expression of the Halophos cluster in a nonpathogenic strain resulted in the ability to cause foliar necrosis on onion.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Virology
Saurabh Gautam, James W. Buck, Bhabesh Dutta, Timothy Coolong, Tatiana Sanchez, Hugh A. Smith, Scott Adkins, Rajagopalbabu Srinivasan
Summary: This study found that Sida golden mosaic virus (SiGMV) collected from prickly sida can infect various crops and weeds. Whiteflies can efficiently transmit SiGMV from infected plants to snap bean. SiGMV can potentially pose a threat to snap bean and other crops in the southeastern United States.
Article
Biochemistry & Molecular Biology
Main Ern Ang, James M. Cowley, Kuok Yap, Michael G. Hahn, Deirdre Mikkelsen, Matthew R. Tucker, Barbara A. Williams, Rachel A. Burton
Summary: Upon wetting, chia nutlets produce a gel-like capsule of mucilage, which is a significant part of their dietary fibre content. The composition and structure of chia nutlet mucilage are complex and contain several types of polymers. The fermentation of chia nutlet mucilage is poor, with only oligosaccharides being fermentable substrates. Grinding chia nutlets improves the fermentation and bioaccessibility of key nutrients, including dietary fibre.
Article
Microbiology
Brendon K. Myers, Gi Yoon Shin, Gaurav Agarwal, Shaun P. Stice, Ronald D. Gitaitis, Brian H. Kvitko, Bhabesh Dutta
Summary: This study investigated the genetic basis of pathogenicity in P. ananatis strains on different Allium species. Phenotype-dependent and phenotype-independent gene association analyses identified genes related to stress responses, toxin-antitoxin systems, flagellar motility, quorum sensing, and phosphonoglycan biosynthesis. Comparative genomics analysis further revealed a putative type III secretion system and other shared genes between different hosts.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gi Yoon Shin, Bhabesh Dutta, Brian Kvitko
Summary: Pantoea ananatis is an unusual bacterial pathogen that causes extensive necrosis in onion foliage and bulb tissues. The onion necrosis phenotype is dependent on the expression of the phosphonate toxin, pantaphos, which is synthesized by putative enzymes encoded by the HiVir gene cluster. In this study, the genetic contributions of individual hvr genes in HiVir-mediated onion necrosis were investigated and the essential hvr genes were characterized in phenotypically deviant strains. The study also demonstrated the importance of necrotic tissues for the proliferation of P. ananatis in onion.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Plant Sciences
Santosh Koirala, Brendon Myers, Gi Yoon Shin, Ron Gitaitis, Brian H. Kvitko, Bhabesh Dutta
Summary: Center rot of onion is a complex disease caused by the plant pathogenic Pantoea species, leading to significant yield losses. Organic growers have limited management options for this disease. The use of peroxide as a pretreatment did not effectively enhance the colonizing ability of biocontrol agents (BCAs) and had limited impact on managing center rot. Field trials showed no significant difference in disease progression between peroxide-pretreated BCAs plots and individual BCA treatments. The foliar microbiome study also indicated lower levels of P. fluorescens retention at the end of the growing season.
Article
Entomology
Swikriti Pandey, Andre Luiz Biscaia Ribeiro da Silva, Bhabesh Dutta, Juang Horng Chong, Martha Ann Mutschler, Jason M. Schmidt
Summary: Acylsugar-producing tomato plants can reduce whitefly populations but not the transmission of Tomato yellow leaf curl virus. Predatory mite release methods did not establish well on tomato plants.
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA
(2023)
Article
Horticulture
Hanna Ibiapina de Jesus, Andre Luiz Biscaia Ribeiro da Silva, Bhabesh Dutta, Kate Cassity-Duffey, Timothy Coolong
Summary: This study evaluates the performance of two commonly used organic fertilizers in the production of Vidalia onions. The results show that increasing the application rate of fertilizer can increase onion yields, but an optimal application rate for organic fertilizers was not determined.
Article
Microbiology
Mei Zhao, Ron Gitaitis, Bhabesh Dutta
Summary: Outbreaks of bacterial leaf spot and blight in pepper and tomato can be caused by novel Pseudomonas species, which have a wide host range and possess various bacterial virulence factors. The management of this pathogen may be challenging.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Saurabh Gautam, Kiran R. Gadhave, James W. Buck, Bhabesh Dutta, Timothy Coolong, Scott Adkins, Alvin M. Simmons, Rajagopalbabu Srinivasan
Summary: This study reveals that the transmission of CuLCrV mediated by whiteflies between host plants is dependent on virus density, and the infection status of the plants has varying effects on transmission. These findings have important implications for epidemics.
Article
Biotechnology & Applied Microbiology
Utku Avci
Summary: The annual temperate grass Brachypodium distachyon is used as a model system for monocot biomass crops and lignocellulosic recalcitrance study. Its cell walls differ from dicot plants and closely resemble those of other grasses. Antibodies against glycan epitopes were used to investigate glycan distribution in Brachypodium's root and leaf tissues. The differences in epitope presence between tissues and cell types provide insights into monocot biomass.
FERMENTATION-BASEL
(2023)
Article
Entomology
Thiago Rutz, Timothy Coolong, Rajagopalbabu Srinivasan, Alton Sparks, Bhabesh Dutta, Clarence Codod, Alvin M. Simmons, Andre Luiz Biscaia Ribeiro da Silva
Summary: This study evaluated the use of reflective silver plastic mulch and an insect row cover as alternative methods to manage whiteflies in zucchini production. The results showed that these methods reduced whitefly populations and improved plant growth and yield. These treatments can be considered ready-to-use integrated pest management practices for growers.
Meeting Abstract
Plant Sciences
V. S. Negi, G. Agarwal, J. Nichols, A. Bennett, R. Srinivasan, B. Dutta