Article
Cell Biology
Rijwan Uddin Ahammad, Tomoki Nishioka, Junichiro Yoshimoto, Takayuki Kannon, Mutsuki Amano, Yasuhiro Funahashi, Daisuke Tsuboi, Md. Omar Faruk, Yukie Yamahashi, Kiyofumi Yamada, Taku Nagai, Kozo Kaibuchi
Summary: Protein phosphorylation plays a critical role in intracellular signaling pathways and physiological functions in the brain. Dysregulation of these pathways is associated with neurodevelopmental disorders. This study developed an online database to provide information on phosphorylation signals and analyzed related pathways.
Article
Biochemistry & Molecular Biology
Jesse Stoop, Erik H. Douma, Marc van Der Vlag, Marten P. Smidt, Lars P. van Der Heide
Summary: Our study provides detailed insights into the regulation of dopamine synthesis, revealing that Ser40 phosphorylation serves as the crucial switch controlling tyrosine hydroxylase activity.
JOURNAL OF NEUROCHEMISTRY
(2023)
Review
Cell Biology
Vanessa M. Ruscetta, Taj J. J. Seaton, Aleen Shakeel, Stanley N. S. Vasconcelos, Russell D. D. Viirre, Marc J. J. Adler, Michael F. F. Olson
Summary: Cytoskeleton organization and dynamics are regulated by post-translational modifications of key target proteins, including the phosphorylation of myosin light chain proteins by MRCK kinases. Compared to ROCK kinases, the contributions of MRCK kinases are less characterized due to the late discovery of selective inhibitors. The recent development of selective MRCK inhibitors has expanded the tools to study MRCK function and shown therapeutic benefits in cancer studies.
Article
Anesthesiology
Attila Keresztes, Keith Olson, Paul Nguyen, Marissa A. Lopez-Pier, Ryan Hecksel, Natalie K. Barker, Zekun Liu, Victor Hruby, John Konhilas, Paul R. Langlais, John M. Streicher
Summary: This study reveals a new function of opioid receptors as a negative feedback brake on opioid-induced analgesia. By using a selective antagonist for the mu and delta opioid receptors (MDOR), the researchers found that the antagonist could enhance the analgesic effect of oxymorphone in mice, suggesting a role for MDOR in regulating pain. Additionally, the study found that the enhancement effect was selective to MDOR and occurred through the repression of Src and CaMKII signal transduction. These findings suggest that MDOR antagonism could be a potential approach to improve clinical opioid therapy.
Article
Cell Biology
Joaquim Javary, Eugenie Goupil, Mathilde Soulez, Evgeny Kanshin, Antoine Bouchard, Ole-Morten Seternes, Pierre Thibault, Jean-Claude Labbe, Sylvain Meloche
Summary: ERK3, an atypical member of the mitogen-activated protein kinase family, has been poorly characterized in terms of its substrates and cellular functions. This study identifies supervillin (SVIL) as a direct substrate of ERK3 and demonstrates its role in regulating myosin II activation and cytokinesis completion in dividing cells. These findings unveil a new function of ERK3 in cell division and highlight its importance in the regulation of cellular processes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Plant Sciences
Qinpei Li, Haiqi Fu, Xiang Yu, Xing Wen, Hongwei Guo, Yan Guo, Jingrui Li
Summary: This study reveals the role of the SOS2-CTR1 regulatory module in promoting the activation of the ethylene signalling pathway and enhancing plant salt tolerance. The phosphorylation of CTR1 by SOS2 at a specific site activates the ethylene signalling response, leading to improved plant salt resistance. Additionally, compromised proteolytic processing of EIN2 and reduced nuclear localization of EIN2-C are observed in the sos2 mutant.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Weikun Jing, Yonghong Li, Shuai Zhang, Xiaofeng Zhou, Junping Gao, Nan Ma
Summary: Aquaporins (AQPs), as an ancient superfamily protein, play a crucial role in maintaining fluid homeostasis in organisms across different environments. This review summarizes the recent findings on the involvement of AQPs in plant cell signal transduction and highlights the unresolved issues for future investigation.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Biochemical Research Methods
Peipei Zhu, Xiaofeng Wu, Ruo-Yu Zhang, Chuan-Chih Hsu, Zhong-Yin Zhang, W. Andy Tao
Summary: Protein phosphatases are important in cellular processes, but studying their substrates and mechanisms is challenging. This study introduces a novel strategy to identify substrates of SHP2 using quantitative proteomics methods, and discovers new substrates.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Multidisciplinary Sciences
Serhan Yilmaz, Marzieh Ayati, Daniela Schlatzer, A. Ercument Cicek, Mark R. Chance, Mehmet Koyuturk
Summary: Mass spectrometry combined with computational algorithms enables high-throughput screening of phosphoproteins and inference of kinase activity. The RoKAI network framework integrates functional information sources to improve activity inference accuracy and identify understudied kinases.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Bozena Klodova, Jan Fila
Summary: Angiosperm mature pollen enters quiescence before pollination, but rapidly activates and grows a pollen tube post-pollination, accompanied by metabolic activity and protein synthesis. Phosphoproteomic studies aim to compare different species' pollen development and discover common proteins involved in root hair growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Pavneet Kaur, Apeksha Anand, Adil Bhat, Jaswinder Singh Maras, Neena Goyal
Summary: The study identified LdMAPK1 target proteins in Leishmania through proteomics, revealing its role in regulating diverse biological processes such as metabolism, signal transduction, replication, transcription, translation, transporters and cytoskeleton/motor proteins. This implies the potential of LdMAPK1 as a therapeutic drug target for treating visceral leishmaniasis.
JOURNAL OF PROTEOMICS
(2021)
Review
Biochemistry & Molecular Biology
Aziz M. Rangwala, Victoria R. Mingione, George Georghiou, Markus A. Seeliger
Summary: Phosphorylation plays a vital role in regulating biological processes, and protein kinases have been extensively studied for their involvement in human health and disease. Some kinases possess additional catalytic functions in addition to phosphotransferase activity, while others have lost their catalytic activity completely. In this study, we analyzed the UniProtKB database for bifunctional protein kinases and focused on those crucial for bacterial and human cellular homeostasis. These kinases have diverse functional roles in environmental sensing, metabolic regulation, immune-host defense, and cell cycle control. This article explores their dual catalytic activities and their contributions to disease pathogenesis.
Article
Plant Sciences
Jun Takeuchi, Kosuke Fukui, Yoshiya Seto, Yousuke Takaoka, Masanori Okamoto
Summary: Small-molecule plant hormones play a crucial role in controlling plant growth, development, differentiation, and environmental responses. Through genomic decoding of model plants with genetic mutants, scientists have elucidated the molecular mechanisms of nine types of plant hormones and identified the structure of hormone receptors. Studies on the three-dimensional structure of ligand-receptor complexes have revealed the details of ligand perception mechanisms.
Article
Biochemical Research Methods
Harumi Kagiwada, Takayuki Kiboku, Hitomi Matsuo, Masashi Kitazawa, Kazuhiko Fukui, Katsuhisa Horimoto
Summary: Cellular protein phosphorylation is crucial for signal transduction, and the newly developed phosphorylation array system effectively assesses the activity of signaling pathways perturbed by external stimuli, offering a new approach to understanding intracellular signal transduction mechanisms.
Review
Biochemical Research Methods
Shaofeng Lin, Chenwei Wang, Jiaqi Zhou, Ying Shi, Chen Ruan, Yiran Tu, Lan Yao, Di Peng, Yu Xue
Summary: Protein phosphorylation is crucial in regulating biological processes in eukaryotes, with a growing number of phosphorylation sites being identified through mass spectrometry-based phosphoproteomics. The development of the eukaryotic phosphorylation site database (EPSD) has provided a comprehensive collection and annotation of phosphorylation sites in eukaryotic proteins, serving as a valuable resource for further analysis in this field.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Microbiology
Hailong Guo, Hee-Kyung Ahn, Jan Sklenar, Jianhua Huang, Yan Ma, Pingtao Ding, Frank L. H. Menke, Jonathan D. G. Jones
CELL HOST & MICROBE
(2020)
Article
Multidisciplinary Sciences
Kathrin Thor, Shushu Jiang, Erwan Michard, Jeoffrey George, Soenke Scherzer, Shouguang Huang, Julian Dindas, Paul Derbyshire, Nuno Leitao, Thomas A. DeFalco, Philipp Koester, Kerri Hunter, Sachie Kimura, Julien Gronnier, Lena Stransfeld, Yasuhiro Kadota, Christoph A. Buecherl, Myriam Charpentier, Michael Wrzaczek, Daniel MacLean, Giles E. D. Oldroyd, Frank L. H. Menke, M. Rob G. Roelfsema, Rainer Hedrich, Jose Feijo, Cyril Zipfel
Article
Multidisciplinary Sciences
Volker Hegenauer, Peter Slaby, Max Koerner, Julien-Alexander Bruckmueller, Ronja Burggraf, Isabell Albert, Bettina Kaiser, Birgit Loeffelhardt, Irina Droste-Borel, Jan Sklenar, Frank L. H. Menke, Boris Macek, Aashish Ranjan, Neelima Sinha, Thorsten Nuernberger, Georg Felix, Kirsten Krause, Mark Stahl, Markus Albert
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Kyle W. Bender, Daniel Couto, Yasuhiro Kadota, Alberto P. Macho, Jan Sklenar, Paul Derbyshire, Marta Bjornson, Thomas A. DeFalco, Annalise Petriello, Maria Font Farre, Benjamin Schwessinger, Vardis Ntoukakis, Lena Stransfeld, Alexandra M. E. Jones, Frank L. H. Menke, Cyril Zipfel
Summary: Receptor kinases are key in extracellular sensing and stress responses in plants, with leucine-rich repeat receptor kinases regulating responses to internal and external stimuli. The phosphorylation of cytoplasmic domains is crucial for activating RK complexes, but it is not a ubiquitous requirement for LRR-RK activation. Further studies on different protein kinase domains may provide insights into the regulation of LRR-RK complexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Xinhua Sun, Dmitry Lapin, Joanna M. Feehan, Sara C. Stolze, Katharina Kramer, Joram A. Dongus, Jakub Rzemieniewski, Servane Blanvillain-Baufume, Anne Harzen, Jaqueline Bautor, Paul Derbyshire, Frank L. H. Menke, Iris Finkemeier, Hirofumi Nakagami, Jonathan D. G. Jones, Jane E. Parker
Summary: Plants utilize intracellular NLR immune receptors to detect pathogen effectors and activate defense mechanisms involving modular networks of downstream proteins. Two distinct modules, NRG1/EDS1/SAG101 and ADR1/EDS1/PAD4, mediate TNL receptor defense signaling in plants, with rapid induced association of non-interchangeable components after NLR activation.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Lida Derevnina, Mauricio P. Contreras, Hiroaki Adachi, Jessica Upson, Angel Vergara Cruces, Rongrong Xie, Jan Sklenar, Frank L. H. Menke, Sam T. Mugford, Dan MacLean, Wenbo Ma, Saskia Hogenhout, Aska Goverse, Abbas Maqbool, Chih-Hang Wu, Sophien Kamoun
Summary: Research has shown that plant NLR proteins can form receptor networks to provide cell death and immunity. Pathogens have evolved proteins to counteract NRC activities and suppress plant defense responses, leading to the continuous evolution of NRC networks.
Article
Microbiology
Miriam Oses-Ruiz, Neftaly Cruz-Mireles, Magdalena Martin-Urdiroz, Darren M. Soanes, Alice Bisola Eseola, Bozeng Tang, Paul Derbyshire, Mathias Nielsen, Jitender Cheema, Vincent Were, Iris Eisermann, Michael J. Kershaw, Xia Yan, Guadalupe Valdovinos-Ponce, Camilla Molinari, George R. Littlejohn, Barbara Valent, Frank L. H. Menke, Nicholas J. Talbot
Summary: Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is a devastating disease that poses a threat to rice production worldwide. Researchers have identified a network of transcription factors that are coregulated downstream of the Pmk1 pathway, regulating gene expression during appressorium-mediated plant infection. This regulatory cascade provides new potential targets for disease intervention in rice plants.
NATURE MICROBIOLOGY
(2021)
Article
Biology
Jack Rhodes, Andra-Octavia Roman, Marta Bjornson, Benjamin Brandt, Paul Derbyshire, Michele Wyler, Marc W. Schmid, Frank L. H. Menke, Julia Santiago, Cyril Zipfel
Summary: This study reports on a previously uncharacterised family of plant signalling peptides, CTNIPs, and identifies the orphan receptor HSL3 as their receptor. The HSL3-CTNIP signalling module is conserved in most extant angiosperms. This discovery is important for understanding the diverse functions of plant signalling peptides and receptor-ligand co-evolution.
Article
Biochemistry & Molecular Biology
Gang Yu, Maria Derkacheva, Jose S. Rufian, Carla Brillada, Kathrin Kowarschik, Shushu Jiang, Paul Derbyshire, Miaomiao Ma, Thomas A. DeFalco, Rafael J. L. Morcillo, Lena Stransfeld, Yali Wei, Jian-Min Zhou, Frank L. H. Menke, Marco Trujillo, Cyril Zipfel, Alberto P. Macho
Summary: A bacterial effector RipAC targets the plant E3 ubiquitin ligase PUB4 to inhibit pattern-triggered immunity (PTI), shedding light on the role played by PUB4 in immune regulation and illustrating an indirect targeting of the immune signaling hub BIK1.
Article
Biochemistry & Molecular Biology
Jiashu Chu, Isabel Monte, Thomas A. DeFalco, Philipp Koster, Paul Derbyshire, Frank L. H. Menke, Cyril Zipfel
Summary: In this study, the researchers discovered that MpRBOH1 and MpPBLa, single members of the RBOH and PBL families in the liverwort Marchantia polymorpha, are essential for chitin-induced ROS production. MpPBLa directly interacts with and phosphorylates MpRBOH1, and this phosphorylation is crucial for chitin-induced ROS production mediated by MpRBOH1.
Article
Biochemistry & Molecular Biology
Hui Li, Jinlon Wang, Tung Ariel Kuan, Bozeng Tang, Li Feng, Jiuyu Wang, Zhi Cheng, Jan Sklenar, Michelle Hulin, Yufei Li, Yi Zhai, Yingnan Hou, Frank L. H. Menke, Yanli Wang, Wenbo Ma
Summary: Pathogens employ a strategy of molecular mimicry to hijack host phosphatase and diversify their effector repertoire by protein modularity. The (L)WY-LWY module in Phytophthora effectors recruits plant PP2A core enzyme to regulate host phosphoproteins. The conservation of the PP2A-interacting module but divergent C-terminal LWY units allows for functional diversity in effector-mediated manipulation of host cellular processes.
Article
Plant Sciences
Yukihisa Goto, Noriko Maki, Jan Sklenar, Paul Derbyshire, Frank L. H. Menke, Cyril Zipfel, Yasuhiro Kadota, Ken Shirasu
Summary: This study reveals a novel negative regulator, PB1CP, for RBOHD and elucidates its possible regulatory mechanisms involving the removal of phosphorylated BIK1 from RBOHD and the promotion of RBOHD endocytosis.
Article
Plant Sciences
Thomas A. DeFalco, Pauline Anne, Sean R. James, Andrew C. Willoughby, Florian Schwanke, Oliver Johanndrees, Yasmine Genolet, Paul Derbyshire, Qian Wang, Surbhi Rana, Anne-Marie Pullen, Frank L. H. Menke, Cyril Zipfel, Christian S. Hardtke, Zachary L. Nimchuk
Summary: This study uncovers a common regulatory mechanism involving a RLCK kinase and a PP2C phosphatase that regulates receptor signaling in both immune responses and plant development.
Letter
Plant Sciences
Lauren E. Grubb, Paul Derbyshire, Katherine E. Dunning, Cyril Zipfel, Frank L. H. Menke, Jacqueline Monaghan
Summary: The analysis focuses on the ubiquitination sites of proteins located at the cell periphery, including a wide range of protein kinases. This study provides valuable insights for further research on protein functions.
Article
Biochemistry & Molecular Biology
Benjamin Petre, Mauricio P. Contreras, Tolga O. Bozkurt, Martin H. Schattat, Jan Sklenar, Sebastian Schornack, Ahmed Abd-El-Haliem, Roger Castells-Graells, Rosa Lozano-Duran, Yasin F. Dagdas, Frank L. H. Menke, Alexandra M. E. Jones, Jack H. Vossen, Silke Robatzek, Sophien Kamoun, Joe Win
Summary: By transiently expressing RXLR effectors of the potato blight pathogen Phytophthora infestans in Nicotiana benthamiana leaves, an effector-host protein interactome matrix was generated, revealing over 35 biological processes potentially targeted by P. infestans. This study provides a valuable resource for functional studies of P. infestans effectors and effector-targeted host processes.
Article
Biochemistry & Molecular Biology
Armando Moreno-Velasco, Mabel Fragoso-Serrano, Pedro de Jesus Flores-Tafoya, Sebastian Carrillo-Rojas, Elihu Bautista, Suzana Guimara Leitao, Jhon F. Castaneda-Gomez, Rogelio Pereda-Miranda
Summary: This study investigated the roots of Operculina hamiltonii, a traditional medicinal plant in Brazil. Three new acyl sugars or resin glycosides were identified and their structures elucidated. The combination of these compounds with other drugs showed enhanced cytotoxic effects on breast cancer cells.
Article
Biochemistry & Molecular Biology
Qian-Qing Liu, Lin-An Xiong, Jia-Yu Qian, Ting-Ting Gong, Lie-Feng Ma, Luo Fang, Zha-Jun Zhan
Summary: A new naturally occurring compound, Linderagatins C-F (1-4), with the structures of diaryltetrahydrofuran-type 7,9'-dinorlignans, was discovered in the roots of Lindera aggregata. The neuroprotective effects of compounds 2 and 3 on erastin-induced ferroptosis in HT-22 cells were found to be significant.
