Reporter cell lines for skin sensitization testing

Assessing skin sensitization hazard in mice and men using non-animal test methods

(2015) Daniel Urbisch et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Human relevance of an in vitro gene signature in HaCaT for skin sensitization

(2015) Jochem W. van der Veen et al.   TOXICOLOGY IN VITRO

A fast Resazurin-based live viability assay is equivalent to the MTT-test in the KeratinoSens assay

(2015) Roger Emter et al.   TOXICOLOGY IN VITRO

Detection of potentially skin sensitizing hydroperoxides of linalool in fragranced products

(2014) Susanne Kern et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Epoxyalcohols: Bioactivation and Conjugation Required for Skin Sensitization

(2014) Tamara Delaine et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Oxidative stress-dependent activation of the eIF2α–ATFr unfolded protein response branch by skin sensitizer 1-fluoro-2,4-dinitrobenzene modulates dendritic-like cell maturation and inflammatory status in a biphasic manner

(2014) Andreia Luís et al.   FREE RADICAL BIOLOGY AND MEDICINE

Heat Shock Proteins HSP27 and HSP70 Are Present in the Skin and Are Important Mediators of Allergic Contact Hypersensitivity

(2014) N. Yusuf et al.   JOURNAL OF IMMUNOLOGY

Evaluating the performance of integrated approaches for hazard identification of skin sensitizing chemicals

(2014) Jochem W. van der Veen et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Predicting Skin Sensitizer Potency Based on In Vitro Data from KeratinoSens and Kinetic Peptide Binding: Global Versus Domain-Based Assessment

(2014) Andreas Natsch et al.   TOXICOLOGICAL SCIENCES

LuSens: A keratinocyte based ARE reporter gene assay for use in integrated testing strategies for skin sensitization hazard identification

(2014) Tzutzuy Ramirez et al.   TOXICOLOGY IN VITRO

Performance of the N/TERT epidermal model for skin sensitizer identification via Nrf2-Keap1-ARE pathway activation

(2014) Mariam Alloul-Ramdhani et al.   TOXICOLOGY IN VITRO

Integrating non-animal test information into an adaptive testing strategy – skin sensitization proof of concept case

(2014) Joanna Jaworska   ALTEX-Alternatives to Animal Experimentation

Development of an in Vitro Dendritic Cell-Based Test for Skin Sensitizer Identification

(2013) Bruno Miguel Neves et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Allergic Skin Inflammation Induced by Chemical Sensitizers Is Controlled by the Transcription Factor Nrf2

(2013) Zeina El Ali et al.   TOXICOLOGICAL SCIENCES

Utility of Rat Liver S9 Fractions to Study Skin-Sensitizing Prohaptens in a Modified KeratinoSens Assay

(2013) Andreas Natsch et al.   TOXICOLOGICAL SCIENCES

Reactivity of Chemical Sensitizers Toward Amino Acids In Cellulo Plays a Role in the Activation of the Nrf2-ARE Pathway in Human Monocyte Dendritic Cells and the THP-1 Cell Line

(2013) Camille Migdal et al.   TOXICOLOGICAL SCIENCES

An epidermal equivalent assay for identification and ranking potency of contact sensitizers

(2013) Susan Gibbs et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Development of a new in vitro skin sensitization assay (Epidermal Sensitization Assay; EpiSensA) using reconstructed human epidermis

(2013) Kazutoshi Saito et al.   TOXICOLOGY IN VITRO

Gene expression changes induced by skin sensitizers in the KeratinoSens™ cell line: Discriminating Nrf2-dependent and Nrf2-independent events

(2013) Roger Emter et al.   TOXICOLOGY IN VITRO

Evaluation of selected biomarkers for the detection of chemical sensitization in human skin: A comparative study applying THP-1, MUTZ-3 and primary dendritic cells in culture

(2013) Manuel Hitzler et al.   TOXICOLOGY IN VITRO

Induction of skin sensitization is augmented in Nrf2-deficient mice

(2012) Jochem W. van der Veen et al.   ARCHIVES OF TOXICOLOGY

Analogues of the Epoxy Resin Monomer Diglycidyl Ether of Bisphenol F: Effects on Contact Allergenic Potency and Cytotoxicity

(2012) Niamh M. O'Boyle et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Allergic contact dermatitis: xenoinflammation of the skin

(2012) Stefan F Martin   CURRENT OPINION IN IMMUNOLOGY

Anin vitromethod for detecting chemical sensitization using human reconstructed skin models and its applicability to cosmetic, pharmaceutical, and medical device safety testing

(2012) James M. McKim et al.   Cutaneous and Ocular Toxicology

Development and validation of a new in vitro assay designed to measure contact allergen-triggered oxidative stress in dendritic cells

(2012) Masaaki Miyazawa et al.   JOURNAL OF DERMATOLOGICAL SCIENCE

Copper(ii) oxide nanoparticles penetrate into HepG2 cells, exert cytotoxicity via oxidative stress and induce pro-inflammatory response

(2012) Jean-Pascal Piret et al.   Nanoscale

Contact Sensitizers Induce Skin Inflammation via ROS Production and Hyaluronic Acid Degradation

(2012) Philipp R. Esser et al.   PLoS One

Elucidation of Xenobiotic Metabolism Pathways in Human Skin and Human Skin Models by Proteomic Profiling

(2012) Sven van Eijl et al.   PLoS One

Putting the parts together: Combining in vitro methods to test for skin sensitizing potentials

(2012) Caroline Bauch et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Applicability of a keratinocyte gene signature to predict skin sensitizing potential

(2012) Jochem W. van der Veen et al.   TOXICOLOGY IN VITRO

Transfer of a two-tiered keratinocyte assay: IL-18 production by NCTC2544 to determine the skin sensitizing capacity and epidermal equivalent assay to determine sensitizer potency

(2012) Marc Teunis et al.   TOXICOLOGY IN VITRO

A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests

(2011) Henrik Johansson et al.   BMC GENOMICS

Impact of a Heteroatom in a Structure−Activity Relationship Study on Analogues of Phenyl Glycidyl Ether (PGE) from Epoxy Resin Systems

(2011) Ida B. Niklasson et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Structure–Activity Relationship between the in Vivo Skin Sensitizing Potency of Analogues of Phenyl Glycidyl Ether and the Induction of Nrf2-Dependent Luciferase Activity in the KeratinoSens in Vitro Assay

(2011) Tamara Delaine et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Evaluating the sensitization potential of surfactants: Integrating data from the local lymph node assay, guinea pig maximization test, and in vitro methods in a weight-of-evidence approach

(2011) Nicholas Ball et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

An In Vitro Test to Screen Skin Sensitizers Using a Stable THP-1–Derived IL-8 Reporter Cell Line, THP-G8

(2011) Toshiya Takahashi et al.   TOXICOLOGICAL SCIENCES

The Role of MAPK and Nrf2 Pathways in Ketanserin-Elicited Attenuation of Cigarette Smoke–Induced IL-8 Production in Human Bronchial Epithelial Cells

(2011) Way Kwok Wai Lau et al.   TOXICOLOGICAL SCIENCES

Intralaboratory validation of four in vitro assays for the prediction of the skin sensitizing potential of chemicals

(2011) Caroline Bauch et al.   TOXICOLOGY IN VITRO

The future of toxicity testing: a focus on in vitro methods using a quantitative high-throughput screening platform

(2010) Sunita J. Shukla et al.   DRUG DISCOVERY TODAY

Adverse outcome pathways: A conceptual framework to support ecotoxicology research and risk assessment

(2010) Gerald T. Ankley et al.   ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY

Lack of the purinergic receptor P2X7results in resistance to contact hypersensitivity

(2010) Felix C. Weber et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Keratinocyte Gene Expression Profiles Discriminate Sensitizing and Irritating Compounds

(2010) Rob J. Vandebriel et al.   TOXICOLOGICAL SCIENCES

Chemical Allergy: Translating Biology into Hazard Characterization

(2010) I. Kimber et al.   TOXICOLOGICAL SCIENCES

Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro

(2010) Roger Emter et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Signal transduction profile of chemical sensitisers in dendritic cells: An endpoint to be included in a cell-based in vitro alternative approach to hazard identification?

(2010) Bruno Miguel Neves et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

In vitro detection of contact allergens: Development of an optimized protocol using human peripheral blood monocyte-derived dendritic cells

(2010) Hendrik Reuter et al.   TOXICOLOGY IN VITRO

The intra- and inter-laboratory reproducibility and predictivity of the KeratinoSens assay to predict skin sensitizers in vitro: Results of a ring-study in five laboratories

(2010) Natsch Andreas et al.   TOXICOLOGY IN VITRO

Prediction of the contact sensitizing potential of chemicals using analysis of gene expression changes in human THP-1 monocytes

(2010) Joanna Arkusz et al.   TOXICOLOGY LETTERS

Characterization of the cancer chemopreventive NRF2-dependent gene battery in human keratinocytes: demonstration that the KEAP1–NRF2 pathway, and not the BACH1–NRF2 pathway, controls cytoprotection against electrophiles as well as redox-cycling compounds

(2009) A.Kenneth MacLeod et al.   CARCINOGENESIS

Reduced Sensitizing Capacity of Epoxy Resin Systems: A Structure−Activity Relationship Study

(2009) Ida B. Niklasson et al.   CHEMICAL RESEARCH IN TOXICOLOGY

High Throughput Kinetic Profiling Approach for Covalent Binding to Peptides: Application to Skin Sensitization Potency of Michael Acceptor Electrophiles

(2009) David W. Roberts et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Oxidation of Cell Surface Thiol Groups by Contact Sensitizers Triggers the Maturation of Dendritic Cells

(2009) Saori Kagatani et al.   JOURNAL OF INVESTIGATIVE DERMATOLOGY

Quantitative DY-maleimide-based proteomic 2-DE-labeling strategies using human skin proteins

(2009) Lisa Dietz et al.   PROTEOMICS

The Nrf2-Keap1-ARE Toxicity Pathway as a Cellular Sensor for Skin Sensitizers—Functional Relevance and a Hypothesis on Innate Reactions to Skin Sensitizers

(2009) Andreas Natsch   TOXICOLOGICAL SCIENCES

Reactivity Profiling: Covalent Modification of Single Nucleophile Peptides for Skin Sensitization Risk Assessment

(2009) Maja Aleksic et al.   TOXICOLOGICAL SCIENCES

Role of protein haptenation in triggering maturation events in the dendritic cell surrogate cell line THP-1

(2009) Rym Megherbi et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Comparative DNA microarray analysis of human monocyte derived dendritic cells and MUTZ-3 cells exposed to the moderate skin sensitizer cinnamaldehyde

(2009) François Python et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Use of IL-18 production in a human keratinocyte cell line to discriminate contact sensitizers from irritants and low molecular weight respiratory allergens

(2009) Emanuela Corsini et al.   TOXICOLOGY IN VITRO

Evaluation of cytochrome P450 1 (CYP1) and N-acetyltransferase 1 (NAT1) activities in HaCaT cells: Implications for the development of in vitro techniques for predictive testing of contact sensitizers

(2009) Jutta Bonifas et al.   TOXICOLOGY IN VITRO

Nrf2 activation by sulforaphane restores the age-related decrease of TH1 immunity: Role of dendritic cells

(2008) Hyon-Jeen Kim et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Depletion of Human Peripheral Blood Lymphocytes in CD25+ Cells Allows for the Sensitive In Vitro Screening of Contact Allergens

(2008) Marc Vocanson et al.   JOURNAL OF INVESTIGATIVE DERMATOLOGY

Role of MAPK signaling pathway in the activation of dendritic type cell line, THP-1, induced by DNCB and NiSO4

(2008) Masaaki Miyazawa et al.   JOURNAL OF TOXICOLOGICAL SCIENCES

Production of IL-8 in THP-1 cells following contact allergen stimulation via mitogen-activated protein kinase activation or tumor necrosis factor-α production

(2008) Yuko Nukada et al.   JOURNAL OF TOXICOLOGICAL SCIENCES

Increased Level of Extracellular ATP at Tumor Sites: In Vivo Imaging with Plasma Membrane Luciferase

(2008) Patrizia Pellegatti et al.   PLoS One

Filling the Concept with Data: Integrating Data from Different In Vitro and In Silico Assays on Skin Sensitizers to Explore the Battery Approach for Animal-Free Skin Sensitization Testing

(2008) Andreas Natsch et al.   TOXICOLOGICAL SCIENCES

LC-MS–Based Characterization of the Peptide Reactivity of Chemicals to Improve the In Vitro Prediction of the Skin Sensitization Potential

(2008) Andreas Natsch et al.   TOXICOLOGICAL SCIENCES

HMOX1 and NQO1 Genes are Upregulated in Response to Contact Sensitizers in Dendritic Cells and THP-1 Cell Line: Role of the Keap1/Nrf2 Pathway

(2008) Nadège Ade et al.   TOXICOLOGICAL SCIENCES

A cell-based in vitro alternative to identify skin sensitizers by gene expression

(2008) Jef Hooyberghs et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Allergic Contact Dermatitis––Formation, Structural Requirements, and Reactivity of Skin Sensitizers

(2007) Ann-Therese Karlberg et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Chemical reactivity indices and mechanism-based read-across for non-animal based assessment of skin sensitisation potential

(2007) David W. Roberts et al.   JOURNAL OF APPLIED TOXICOLOGY

A review of critical factors in the conduct and interpretation of the human repeat insult patch test

(2007) Pauline M. McNamee et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Role of p38 MAPK in the selective release of IL-8 induced by chemical allergen in naïve THP-1 cells

(2007) Montserrat Mitjans et al.   TOXICOLOGY IN VITRO