Article
Biochemistry & Molecular Biology
Shin-Ping Lin, Ting-Hsuan Sung, Artik Elisa Angkawijaya, Alchris Woo Go, Chang-Wei Hsieh, Hsien-Yi Hsu, Shella Permatasari Santoso, Kuan-Chen Cheng
Summary: Repeated-batch fermentation with fungal mycelia immobilized in plastic composite support (PCS) reduces lag phase and increases metabolite productivity. This study developed a modified PCS to enhance exopolysaccharide (EPS) production from Cordyceps militaris. The use of SYE + PCS led to consistent cell productivity and higher cell productivity compared to unmodified PCS. The EPS yield and cordycepin content from mycelium grown on SYE + PCS were significantly higher than those on unmodified PCS.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Sujarinee Sangwanna, Weerasak Seelarat, Teerapap Panklai, Natthaphon Chaosuan, Ahkasit Subcharoen, Nuttapon Subcharoen, Nattapong Chanchula, Tanapak Inyod, Thanaphat Toemarrom, Atipong Bootchanont, Chakkaphan Wattanawikkam, Sorapong Pavasupree, Dheerawan Boonyawan, Porramain Porjai
Summary: This study investigated the effects of different treatment times of an air atmospheric pressure plasma jet (APPJ) on the production of fruiting bodies and phytochemicals of white Cordyceps militaris. Results showed that optimal APPJ treatment significantly increased the fresh and dry weights of white C. militaris fruiting bodies. APPJ treatments also led to higher levels of cordycepin, adenosine, superoxide dismutase, glutathione S-transferase, and beta-glucan in the fruiting bodies, as well as enhanced antioxidant properties. However, carotenoid content decreased with APPJ treatment.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Gongbo Lv, Yue Zhu, Xiaojie Cheng, Yan Cao, Bin Zeng, Xinping Liu, Bin He
Summary: This study explored the effects of salt treatments on Cordyceps militaris and found that increased salt concentration can significantly enhance cordycepin production. Transcriptome analysis revealed differentially expressed genes related to salt adaptation and cordycepin biosynthesis. Overall, this research provides insights for developing industrial strains with high cordycepin production and salt tolerance in C. militaris.
FRONTIERS IN NUTRITION
(2021)
Article
Chemistry, Multidisciplinary
Yoonjin Park, Seoyoon Choi, Boyong Kim, Seung Gwan Lee
Summary: The study demonstrates that Cordyceps militaris extract has strong effects on modulating macrophages, dendritic cells, and T cells under inflammatory stress, promoting M2 polarization and attenuating M1 polarization. It also enhances macrophage phagocytosis and promotes differentiation of dendritic cells towards CD11b(+) and Xcr1(+) cells, as well as T-reg and natural killer T-like (NKTL) cells.
APPLIED SCIENCES-BASEL
(2021)
Review
Food Science & Technology
Karol Jerzy Jedrejko, Jan Lazur, Bozena Muszynska
Summary: Due to their adaptogenic, tonic effects and ability to reduce fatigue and stimulate the immune system in humans, Cordyceps militaris mushrooms contain bioactive compounds like cordycepin, polysaccharides, gamma-aminobutyric acid (GABA), ergothioneine, making them suitable as functional foods and potential for medicinal use. Scientific reports also suggest the antiviral activity of cordycepin, promising in combating diseases like COVID-19.
Article
Biotechnology & Applied Microbiology
Li Wang, Huanhuan Yan, Bin Zeng, Zhihong Hu
Summary: This study provides a comprehensive review of the chemical synthesis methods, biosynthesis pathways, fermentation optimization, and genetic modifications of C. militaris and its major bioactive component COR. The research progress of genetic modifications of C. militaris and methods for separation and purification COR are also introduced. The existing problems and future research direction of C. militaris are discussed. The study serves as a reference for the future production of COR.
BIOENGINEERING-BASEL
(2022)
Article
Microbiology
Tatsuya Kato, Konomi Nishimura, Ahmad Suparmin, Kazuho Ikeo, Enoch Y. Park
Summary: The study showed that Cordyceps militaris produces cordycepin which enhances its pathogenicity towards silkworm larvae, impacting the expression of certain genes in the silkworm larvae.
Article
Biochemistry & Molecular Biology
Karol Jedrejko, Katarzyna Kala, Katarzyna Sulkowska-Ziaja, Agata Krakowska, Piotr Zieba, Krystian Marzec, Agnieszka Szewczyk, Agnieszka Sekara, Jolanta Pytko-Polonczyk, Bozena Muszynska
Summary: This study aimed to determine the content of biologically active substances, total phenolic content, and antioxidant activity of Cordyceps militaris fruiting bodies, mycelia, and food supplements. The results showed that substrate composition and cultivation method influenced the properties of the mushroom materials. An important aspect of the study was the evaluation of bioactive substance content after extraction in a simulated digestive system, which allowed for determining potential bioavailability. The mycelium and fruiting bodies of C. militaris were found to be ideal food supplements and pharmaceutical agents that could provide prohealth substances for humans.
Article
Biotechnology & Applied Microbiology
Bingjie Zhao, Yong Zhang, Sasa Zhang, Ting Hu, Yanbin Guo
Summary: This study investigates the factors influencing cordycepin production in Cordyceps militaris and finds that regulating the glycolysis, pentose phosphate, and histidine metabolism pathways can significantly increase the production of cordycepin. The gene expression of several key enzymes in these pathways is affected by multifactorial interactions. Additionally, the study reveals that increasing cordycepin production can impact glucose uptake, mycelia growth, and cell wall integrity.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Ankang Li, Yiqiao Song, Can Wang, Jun Wang
Summary: This study quantitatively analyzed the contents of cordycepin and its arabinoside in Cordyceps militaris using high-performance liquid chromatography with tandem mass spectrometry. The results showed an upward trend in the content of cordycepin and its arabinoside with growth, reaching the highest level at the mature stage.
Article
Green & Sustainable Science & Technology
Khamphe Phoungthong, Waraporn Aiphuk, Tharakorn Maneerat, Thitipone Suwunwong, Patcharanan Choto, Putarak Chomnunti
Summary: This study investigates the effects of adding different conditions of biochar on cordycepin and adenosine production in Cordyceps militaris. The results show that mixing 10 g of biochar with a rice berry medium is the optimal condition for the highest cordycepin yield and dry fruiting body weight.
Article
Agricultural Engineering
Liangkun Long, Zhen Liu, Yizhou Wang, Qunying Lin, Shaojun Ding, Chuanhua Li, Chunying Deng
Summary: This study identified a xylose-utilising strain of C. militaris that produced a high level of cordycepin in xylose culture. Optimal combination of xylose, alanine, and ammonium citrate was determined using response surface methodology, resulting in the maximum production of cordycepin. Transcriptome analysis revealed the impact of xylose utilisation on fungal metabolism and cordycepin biosynthesis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ayman Turk, Solip Lee, Sang Won Yeon, Se Hwan Ryu, Yoo Kyong Han, Young Jun Kim, Sung Min Ko, Beom Seok Kim, Bang Yeon Hwang, Ki Yong Lee, Mi Kyeong Lee
Summary: In this study, eight medicinal plants were supplemented with cultivation substrates of Cordyceps militaris to increase cordycepin content. Among them, Mori Folium showed the highest enhancement, increasing cordycepin content up to 4 times. Additionally, the study identified medicinal plants that could strongly inhibit ADA activity, suggesting a potential therapeutic strategy.
Article
Pharmacology & Pharmacy
Jian Chen, Hai-Fang Li, Guozhu Zhao, Jin-Ming Lin, Xiangwei He
Summary: Cordycepin, a secondary metabolite of Cordyceps militaris, exhibits various immunomodulatory activities. To improve production, researchers used MALDI-MS detection combined with isotope-labeled internal standard to measure cordycepin content in the fermentation broth and mycelium, achieving accurate quantification of cordycepin.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Jinyan Yu, Min Sun, Xiaoyu Wang, Dongmei Qi, Chunchao Han
Summary: This study obtained high-yielding strains of cordycepin through ultraviolet mutagenesis and found that the protein content increased while the polysaccharide content decreased in these strains. Metabolomics results showed a decrease in the relative content of most metabolites in the high-yielding cordycepin strains. Various metabolic pathways, such as the citric acid cycle, purine metabolism, and pyrimidine metabolism, as well as changes in metabolic poly-pathways related to polysaccharide and protein synthesis, promoted an increase in cordycepin content.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Review
Pharmacology & Pharmacy
Hardeep Singh Tuli, Vivek Kumar Garg, Ajay Kumar, Diwakar Aggarwal, Uttpal Anand, Nidarshana Chaturvedi Parashar, Adesh K. Saini, Ranjan K. Mohapatra, Kuldeep Dhama, Manoj Kumar, Tejveer Singh, Jagjit Kaur, Katrin Sak
Summary: This review article provides a comprehensive description of the potential applications of Oroxylin A (OA), a constituent of Scutellariae plants, in the treatment of malignant tumors. It discusses the various mechanisms of action, including antiproliferative, anti-inflammatory, and proapoptotic effects, and explores the synergistic effects with conventional anticancer agents as well as the advantages of nanotechnological methods in OA application.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dhruv Sanjay Gupta, Vaishnavi Gadi, Ginpreet Kaur, Meena Chintamaneni, Hardeep Singh Tuli, Seema Ramniwas, Gautam Sethi
Summary: The growing incidence of B cell malignancies worldwide has led to research on the pharmacological properties of phytoconstituents in cancer management. Resveratrol, a polyphenolic stilbenoid found in nature, has shown promising anti-inflammatory and antioxidant properties, making it a potential candidate for managing B cell malignancies. However, more clinical trials in diverse populations are needed to establish its safety and efficacy profile, and exploring nanodelivery methods can enhance its bioavailability. This review provides an overview of the occurrence and pathogenesis of B cell malignancies, the mechanism of actions of resveratrol, results from pre-clinical and clinical studies, and discusses the challenges and future perspectives in the development and use of resveratrol as a therapeutic agent.
Review
Chemistry, Medicinal
Suneel Kumar, Stephen O. Mathew, Ravindra Prasad Aharwal, Hardeep Singh Tulli, Chakrabhavi Dhananjaya Mohan, Gautam Sethi, Kwang-Seok Ahn, Kassidy Webber, Sardul Singh Sandhu, Anupam Bishayee
Summary: Cancer is a highly significant global health concern, and current treatment options, such as surgery, chemotherapy, radiation therapy, and immune therapy, have limitations and may lead to disease recurrence. Withaferin A, a natural bioactive compound found in the Indian medicinal plant Withania somnifera, has shown promising anticancer activities by modulating the expression and activity of oncogenic proteins. This article comprehensively discusses the biosynthesis, antineoplastic activities, mode-of-action, and clinical potential of withaferin A, as well as its effect on miRNA expression, combinational use with other cytotoxic agents, formulations, and safety profiles.
Review
Biotechnology & Applied Microbiology
Bharat Singh, Ankit Kumar, Adesh Kumar Saini, Reena Vohra Saini, Rahul Thakur, Shakeel A. Mohammed, Hardeep Singh Tuli, Vijai Kumar Gupta, Mohammed Y. Areeshi, Hani Faidah, Naif A. Jalal, Shafiul Haque
Summary: The demand for bioactive molecules for various commercial products is increasing due to the limitations and associated toxicity of synthetic chemical products. Traditional methods have limited productivity in natural scenarios, but microbial cell factories can improve production yields and screen promising structural homologues of natural molecules. By using cell engineering approaches and new technologies such as high throughput OMICs tools and genome editing, the robustness of microbial hosts can be enhanced to accelerate the production of biomolecules for commercial purposes.
BIOTECHNOLOGY AND GENETIC ENGINEERING REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Damanjeet Kaur, Varruchi Sharma, Amit Joshi, Navneet Batra, Seema Ramniwas, Anil K. Sharma
Summary: Pectinases are a group of enzymes with a common substrate, pectin, which can act on different parts of pectin due to its structural heterogeneity. They are naturally present in both multicellular and unicellular organisms. In recent years, the use of chemical and mechanical methods in industries has led to environmental hazards and health disorders, increasing the demand for eco-friendly approaches. Microbial enzymes, especially pectinases, have been widely used as safer alternatives in various industries. This review focuses on the structure of pectin, microbial sources of pectin, and the industrial applications of pectinases.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Rippin, Anil Sharma, Vikas Beniwal
Summary: Condensed and hydrolyzable tannins in various fruits can be hydrolyzed by tannase enzyme, resulting in the production of gallic acid and tannase-mediated products with potential therapeutic benefits. Prunus domestica and Plum had the highest phenol and hydrolyzable tannin contents, respectively. The hydrolysis of fruit extracts led to increased antioxidant and antibacterial activities.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Hardeep Singh Tuli, Hemant Joshi, Kanupriya Vashishth, Seema Ramniwas, Mehmet Varol, Manoj Kumar, Isha Rani, Vibha Rani, Katrin Sak
Summary: This review article discusses the anticancer properties of a natural biflavone compound called amentoflavone, derived from various plants such as ginkgo, hinoki, and St. John`s wort. It has been found to suppress malignant growth, induce apoptosis in tumor cells, and inhibit angiogenesis and metastasis. Amentoflavone shows promise as a potential lead compound for the development of novel anticancer drugs, and further in vivo studies and clinical trials are warranted.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Suneel Kumar, Nitin Swamy, Hardeep Singh Tuli, Seema Rani, Abhijeet Garg, Deepa Mishra, Hadi Sajid Abdulabbas, Sardul Singh Sandhu
Summary: The global fight against the deadliest cancer sickness is being fought using various treatment options such as chemotherapy, hormonal therapy, surgery, and radiation therapy. However, these treatments have drawbacks, leading to the search for natural phytochemical substances. Various phytochemical compounds have shown promising results against deadly diseases, offering considerable advantages in terms of safety and cost-effectiveness.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Hemant Joshi, Dhruv Sanjay Gupta, Nosheen Kamruddin Abjani, Ginpreet Kaur, Chakrabhavi Dhananjaya Mohan, Jagjit Kaur, Diwakar Aggarwal, Isha Rani, Seema Ramniwas, Hadi Sajid Abdulabbas, Madhu Gupta, Hardeep Singh Tuli
Summary: Genistein, a commonly occurring isoflavone, has gained popularity for its diverse pharmacological benefits, including improved bone health, reduced postmenopausal complications, and anti-cancer potential. This review provides insights into its usage, chemistry, pharmacokinetics, apoptotic mechanisms in cancer management, opportunities for synergism, and recent clinical trials. Additionally, advancements in nanotechnology for improved safety and efficacy and results from safety and toxicology studies are discussed.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Shivani Attri, Ajay Kumar, Kirandeep Kaur, Prabhjot Kaur, Sanha Punj, Neena Bedi, Hardeep Singh Tuli, Saroj Arora
Summary: The aim of this study was to evaluate the anti-psoriatic potential of bakuchiol loaded solid lipid nanoparticles (SLNs) by modulating inflammatory and oxidative pathways. Bak-loaded SLNs were prepared and characterized. The release studies confirmed sustained release of Bak-SLNs-based gel. In a UV-B-induced psoriatic rat model, Bak showed significant anti-psoriatic effect via regulating inflammatory markers and levels of antioxidant enzymes. RT-qPCR analysis confirmed the downregulation of inflammatory markers by Bak. The study indicates that Bak-loaded SLNs-based gel can be a novel therapeutic approach for psoriasis.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Poonam Bansal, Varruchi Sharma, Anil Panwar, Raman Kumar, Aanchal Sharma, Seema Ramniwas, Abhijit Dey, Deepak Chandran, Kuldeep Dhama, Anil K. Sharma
Summary: The emergence of the Omicron variant has posed challenges in containing SARS-CoV-2 infection, but a study suggests that Silybin may be used as a therapeutic agent to effectively inhibit viral entry into human cells by blocking the interaction between the spike protein and the host ACE2 receptor.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2023)
Article
Immunology
Sirwan Khalid Ahmed, Salar Omar Abdulqadir, Rukhsar Muhammad Omar, Ardalan Jabbar Abdullah, Hawre Asaad Rahman, Safin Hassan Hussein, Halkawt Ismail Mohammed Amin, Deepak Chandran, Anil Kumar Sharma, Kuldeep Dhama, Malik Sallam, Harapan Harapan, Nader Salari, Chiranjib Chakraborty, Araz Qadir Abdulla
Summary: The general population in the Kurdistan region of Iraq has a moderate level of knowledge, a neutral attitude, and a moderate level of worry about monkeypox. Factors such as age, gender, religion, education level, and residential area are associated with knowledge, attitude, and worry toward monkeypox.
Article
Biotechnology & Applied Microbiology
Palvi Sharma, Ankita Rajput, Nitish Kumar, Sharabjit Singh, Sukhprit Singh, Hardeep S. Tuli, Shafiul Haque, Rinaldo Pellicano, Sharmila Fagoonee, Saroj Arora
Summary: This study found that Hibiscus rosa-sinensis flower extracts have antioxidant and inhibitory activity against α-amylase and α-glucosidase, which can help attenuate chronic problems caused by hyperglycemia. Among the extracts, the ethyl acetate extract showed the strongest antioxidant and enzyme inhibitory activity.
MINERVA BIOTECHNOLOGY AND BIOMOLECULAR RESEARCH
(2023)
Review
Biotechnology & Applied Microbiology
Rashmi Bangarh, Chainika Khatana, Simranjeet Kaur, Anchita Sharma, Ankur Kaushal, Samarjeet Singh Siwal, Hardeep Singh Tuli, Kuldeep Dhama, Vijay Kumar Thakur, Reena Saini, Adesh K. Saini
Summary: Glycosylation-mediated post-translational modification plays a critical role in regulating fundamental processes and can be associated with diseases like cancer. Changes in glycosylation patterns can lead to heterogeneity in tumor cells and affect immune cells, resulting in pro-inflammatory or anti-inflammatory effects. Understanding glycosylation can be used for diagnosis, prognosis, and immunotherapy in cancer. Advanced technologies like lectin microarrays enable high-throughput research on aberrant glycosylation. This paper reviews the role of glycosylation and discusses emerging technologies and clinical applications.
BIOTECHNOLOGY ADVANCES
(2023)
Review
Multidisciplinary Sciences
Neel Parekh, Anjali Bhagat, Binith Raj, Raunak Singh Chhabra, Harpal Singh Buttar, Ginpreet Kaur, Seema Ramniwas, Hardeep Singh Tuli
Summary: Artificial intelligence has the potential to revolutionize the detection, monitoring, and management of Huntington's disease through applications such as medical image analysis and predictive analytics. AI-driven algorithms can analyze brain imaging data to detect subtle changes and biomarkers, enabling early diagnosis. Additionally, AI's predictive analytics capabilities can analyze disease progression and forecast clinical outcomes, assisting doctors in treatment decisions and care planning.
BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES
(2023)
