Article
Multidisciplinary Sciences
Riccardo Vago, Giorgia Radano, Davide Zocco, Natasa Zarovni
Summary: Urine is an ideal source of non-invasive diagnostic markers, however, issues such as urine concentration variability and biases introduced in urine EV analysis restrict its full potential. This study evaluated methods to decrease biases and found that appropriate urine storage, optimal single-step EV purification method, and normalization method can enable accurate analysis of urine EV RNA.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yiru Wang, Shijie Zhang, Yang Bai, Gen Li, Siyu Wang, Jiayi Chen, Xin Liu, Hang Yin
Summary: This study identified differentially expressed ferroptosis-related lncRNAs and constructed a prediction model consisting of five lncRNAs. The model showed good predictive ability and was confirmed as an independent prognostic indicator. The study also found that these lncRNAs may be regulated by m6A modification in bladder cancer. Patients in the low-risk group were predicted to have a higher response rate to immunotherapy, while those in the high-risk group were predicted to be more sensitive to traditional chemotherapeutic agents.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Urology & Nephrology
Yanish Soorojebally, Yann Neuzillet, Mathieu Roumiguie, Pierre-Jean Lamy, Yves Allory, Francoise Descotes, Sophie Ferlicot, Diana Kassab-Chahmi, Stephane Oudard, Xavier Rebillard, Catherine Roy, Thierry Lebret, Morgan Roupret, Francois Audenet
Summary: This systematic review evaluated the clinical validity and utility of eleven urinary biomarkers for bladder cancer diagnosis and NMIBC follow-up. The results suggested that urinary biomarkers may have a complementary role in bladder cancer diagnosis and NMIBC surveillance, but further confirmation of their clinical benefit is needed.
WORLD JOURNAL OF UROLOGY
(2023)
Review
Genetics & Heredity
Molly Davies, Matthew G. Davey, Nicola Miller
Summary: Ovarian cancer is a common malignancy in women, and early diagnosis is crucial for favorable outcomes. MicroRNAs, which regulate gene expression, have potential as biomarkers for diagnosis and treatment strategies in ovarian cancer.
Review
Pathology
Madelyn Lew
Summary: Urine cytology is an economical and convenient method for screening patients with urinary symptoms and monitoring those previously diagnosed with urothelial carcinoma. Familiarity with nonurothelial malignancies in urine cytology allows for optimal patient care.
ADVANCES IN ANATOMIC PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jen-Tai Lin, Kuo-Wang Tsai
Summary: The study showed that certain miRNAs in urine could potentially serve as noninvasive biomarkers for diagnosing bladder cancer, with implications for early detection and patient survival. Further validation in larger cohorts is necessary to confirm the clinical utility of urinary miRNAs in bladder cancer diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Stefan Harsanyi, Zuzana Varchulova Novakova, Katarina Bevizova, Lubos Danisovic, Stanislav Ziaran
Summary: Bladder cancer is the 10th most common cancer worldwide, with a variety of diagnostic methods available, including standard cystoscopy and urine cytology. Liquid biopsy as a new trend is proving to be almost equally effective in detecting bladder cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Kamala Vanarsa, Jessica Castillo, Long Wang, Kyung Hyun Lee, Claudia Pedroza, Yair Lotan, Chandra Mohan
Summary: This study identified potential biomarkers for bladder cancer diagnosis and disease progression using aptamer screening and systems biology analysis. Urine d-dimer showed high sensitivity (97%) for bladder cancer diagnosis, while urine IL-8 and IgA were associated with disease progression. These biomarkers could be used for initial triage and patient follow-up, reducing the need for invasive cystoscopy-based diagnostic methods.
Review
Oncology
Ekaterina Laukhtina, Sung Ryul Shim, Keiichiro Mori, David D'Andrea, Francesco Soria, Pawel Rajwa, Hadi Mostafaei, Eva Comperat, Alessia Cimadamore, Marco Moschini, Jeremy Yuen-Chun Teoh, Dmitry Enikeev, Evanguelos Xylinas, Yair Lotan, Juan Palou, Paolo Gontero, Marko Babjuk, J. Alfred Witjes, Ashish M. Kamat, Morgan Roupret, Shahrokh F. Shariat, Benjamin Pradere
Summary: The study assessed the diagnostic estimates of novel commercially available UBTs for NMIBC diagnosis and surveillance, showing high diagnostic accuracy and potential to reduce unnecessary cystoscopic examinations.
EUROPEAN UROLOGY ONCOLOGY
(2021)
Review
Genetics & Heredity
Ishaq Tantray, Rani Ojha, Aditya P. Sharma
Summary: A significant portion of the human genome is transcribed into non-coding RNA (ncRNA), which plays a role in various diseases including cancer. In bladder cancer, non-coding RNAs have been identified to be involved in processes such as autophagy, metastasis, drug resistance, and tumor proliferation. Further molecular characterization is needed to explore new diagnostic and treatment options for bladder cancer, utilizing the potential of non-coding RNA as targeted tools and therapeutic approaches.
FRONTIERS IN GENETICS
(2023)
Article
Chemistry, Analytical
Xiaowei Wei, Lijun Cai, Hanxu Chen, Luoran Shang, Yuanjin Zhao, Weijian Sun
Summary: This study proposes a novel system for multiplexed analysis of bladder cancer-derived urine exosomes using magnetic microspheres as barcoded microcarriers. The unique structure of the microcarriers allows for controllable movement and high sensitivity detection of exosomes. The noninvasive nature of the platform, combined with its good sensitivity, specificity, low sample consumption, and ease of use, indicates its great potential for bladder cancer diagnosis in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Review
Plant Sciences
Yi Ren, Yue Song, Lipeng Zhang, Dinghan Guo, Juan He, Lei Wang, Shiren Song, Wenping Xu, Caixi Zhang, Amnon Lers, Chao Ma, Shiping Wang
Summary: Peptides composed of a short chain of amino acids play important roles in plant growth, development, and stress responses, derived from precursor proteins or small open reading frames. They act as local signal molecules for intercellular communication and ligands for cellular signaling transduction. Recent studies have shown that non-coding RNAs can also encode peptides, such as miRNA-encoded peptides (miPEPs), which can increase the expression of corresponding miRNAs by enhancing MIRNA gene transcription.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Klaudia Klicka, Tomasz M. Grzywa, Alicja Klinke, Aleksandra Mielniczuk, Jaroslaw Wejman, Joanna Ostrowska, Agata Gondek, Pawel K. Wlodarski
Summary: This study aimed to assess the expression levels of 16 miRNAs in endometrial cancer and found aberrant expression profiles in some miRNAs. This research is important for understanding the epigenetic mechanisms and potential development of endometrial cancer.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Jinshan Xu, Shuxiong Zeng, Jun Li, Li Gao, Wenjun Le, Xin Huang, Guandan Wang, Bingdi Chen, Zhensheng Zhang, Chuanliang Xu
Summary: The study developed a novel UCT method called Nano-cytology by mixing urine with mNPs to improve the sensitivity of bladder cancer detection. The Nano-cytology assay showed significantly higher sensitivity compared to the UCT assay, and the combination of the two approaches further increased sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Jinbo Song, Xiaoke Sun, Ting Wang, Li Yan, Pengxiao Su, Leihong Yuan
Summary: A predictive signature based on 9 cuproptosis-related lncRNAs has been constructed for bladder cancer (BLCA) patients, which can serve as a promising prognostic biomarker. Patients in the high-risk group show significant differences in prognosis, with higher tumor immune dysfunction and exclusion (TIDE) scores, and lower tumor mutation burden (TMB). There is also a statistically significant difference in the response to antitumor drugs between the low- and high-risk groups.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Genetics & Heredity
Marwa Matboli, Doaa Ibrahim, Amany H. Hasanin, Mohamed Kamel Hassan, Eman K. Habib, Miram M. Bekhet, Ahmed M. Afifi, Sanaa Eissa
Summary: The study demonstrated the significant nephroprotective effect of isorhamnetin in a Type 2 diabetes mellitus rat model, showing improvements in blood glucose and lipid profiles, as well as modulation of autophagy-related genes and miRNA regulation.
Article
Medicine, Research & Experimental
Shaimaa H. Gadallah, Sanaa Eissa, Hala M. Ghanem, Emad K. Ahmed, Amany Helmy Hasanin, Manal M. El Mahdy, Marwa Matboli
Summary: The prophylactic efficacy of gut microbiota-based treatments on nonalcoholic steatohepatitis (NASH) management was investigated through modulation of Hippo signaling pathway-related genes and their epigenetic regulators. Both probiotic mixture and prebiotic inulin fiber attenuated steatosis, inflammation, and fibrosis in NASH rats induced by a high sucrose high fat diet. The treatments also led to improvements in liver function and modulation of RNA panel related to NASH progression.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Marwa Matboli, Marwa Mostafa Kamel, Nada Essawy, Meram Mohamed Bekhit, Basant Abdulrahman, Ghada F. Mohamed, Sanaa Eissa
Summary: The study constructed a ceRNA network associated with insulin resistance, validated its expression in clinical samples and cell lines, and successfully knocked out a specific lncRNA using CRISPR/Cas9. The findings highlight the significance of this network as a therapeutic target for gene editing in T2DM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marwa Matboli, Shaimaa H. Gadallah, Wafaa M. Rashed, Amany Helmy Hasanin, Nada Essawy, Hala M. Ghanem, Sanaa Eissa
Summary: This study aimed to construct a bioinformatics-based co-regulatory network of mRNAs and ncRNAs in NAFLD, which was validated in an animal model. The network revealed key players involved in NAFLD pathogenesis, with significant differences in expression levels between NAFLD and normal controls. This provides valuable insights for potential diagnostic and management strategies for NAFLD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Sara H. A. Agwa, Marwa Mostafa Kamel, Hesham Elghazaly, Aya M. Abd Elsamee, Hala Hafez, Samia Abdo Girgis, Hoda Ezz Elarab, Fatma S. E. Ebeid, Safa Matbouly Sayed, Lina Sherif, Marwa Matboli
Summary: This study investigated the impact of host genetic variation on COVID-19 infection and disease severity, revealing that certain genotypes may make individuals more susceptible and lead to a more severe illness. The A allele in the DDR1 gene rs4618569 variant was associated with disease deterioration.
Article
Cell Biology
Sara H. A. Agwa, Sherif Samir Elzahwy, Mahmoud Shawky El Meteini, Hesham Elghazaly, Maha Saad, Aya M. Abd Elsamee, Rania Shamekh, Marwa Matboli
Summary: The study assessed the predictive power of potential RNA panel expression in acute coronary syndrome, showing that the ABHD4-regulating RNA panel could be potential biomarkers for predicting ACS and stratifying the severity of the condition early. There was a significant association between the two miRNAs and ABHD4 mRNA, as well as the regulating FENDRR lncRNA.
Article
Biochemistry & Molecular Biology
Reda Albadawy, Sara H. A. Agwa, Eman Khairy, Maha Saad, Naglaa El Touchy, Mohamed Othman, Marwa Matboli
Summary: This pilot study evaluated the potential of the Nod-like receptor (NLR) signaling pathway related RNA panel in diagnosing non-alcoholic steatohepatitis (NASH). Upregulation of Lnc-SPARCL1-1:2 lncRNA led to increased levels of miR-6881-5P and subsequent mRNA levels of HSPD1, MMP14, and ITGB1 were observed. ROC curve analysis showed discriminative cutoff values that aided in distinguishing NASH cases from controls, as well as differentiating between NAFLD, simple steatosis, and NASH.
Article
Pharmacology & Pharmacy
Marwa Matboli, Mohmed Kamal Hassan, Mahmoud A. Ali, Mohamed Tarek Mansour, Waheba Elsayed, Reham Atteya, Hebatallah Said Aly, Mahmoud El Meteini, Hesham Elghazaly, Sherif El-Khamisy, Sara H. A. Agwa
Summary: Hepatocellular carcinoma (HCC) is a major cause of cancer-related death in Egypt. This study reveals a novel axis involving hsa-circ-0000221, miR-661, and PTPN11 mRNA in HCC pathogenesis, suggesting a tumor-suppressive role of hsa-circ-0000221 through miR-661 inhibition and subsequent PTPN11 mRNA increase. The findings highlight the potential use of circRNAs as molecular biomarkers in cancer research.
Article
Biochemistry & Molecular Biology
Sara H. A. Agwa, Hesham Elghazaly, Mahmoud Shawky El Meteini, Yahia A. Yahia, Radwa Khaled, Aya M. Abd Elsamee, Reham M. Darwish, Shaimaa M. Elsayed, Hala Hafez, Basma S. Mahmoud, E. M. Fouda, Marwa Matboli
Summary: The SARS-CoV-2 pandemic has resulted in over 4.9 million deaths, posing challenges in developing vaccines, treatments, and diagnostic tools due to the virus's mutations and emerging variants. In this study, we sequenced the viral RNA of 35 confirmed SARS-CoV-2 patients and identified a seven-mutation signature for the C36 lineage, which is detected in 56 countries and has emerged in Egypt. However, no significant correlation was found between the clinical outcomes and the C36 lineage.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medicine, Research & Experimental
Noha M. Hany, Sanaa Eissa, Manal Basyouni, Amany H. Hasanin, Yasmin M. Aboul-Ela, Nagwa M. Abo Elmagd, Iman F. Montasser, Mahmoud A. Ali, Paul J. Skipp, Marwa Matboli
Summary: This study constructed a genetic-epigenetic network linked to hepatic stellate cell (HSC) signaling and evaluated the effects of Mutaflor (R) on this network. The results showed that Mutaflor (R) administration minimized the alterations caused by NAFLD/NASH and reduced HSC activation and profibrogenic protein expression. This effect may be achieved through upregulation of RPARP AS-1 LncRNA and downregulation of miR-650, FOXA2 mRNA, and TEAD2 mRNA expression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Reda Albadawy, Amany Helmy Hasanin, Sara H. A. Agwa, Shaimaa Hamady, Yasmin M. Aboul-Ela, Mona Hussien Raafat, Samaa Samir Kamar, Mohamed Othman, Yahia A. Yahia, Marwa Matboli
Summary: This study investigated the pharmacological activity and potential mechanism of rosavin treatment on non-alcoholic steatohepatitis (NASH) by targeting hepatic cell death-related mRNAs and their upstream noncoding RNA regulators in NASH rats. The results showed that rosavin modulated the expression of hepatic cell death-related RNA panel and improved liver functions, lipid profile, hepatic inflammation, fibrosis, and apoptosis in NASH rats. The study suggests that rosavin may have the potential to attenuate disease progression and inhibit hepatic cell death in NASH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hebatalla Said Ali, Mariam Sameh Boshra, Sara H. A. Agwa, Mohamed S. Abdel Hakeem, Mahmoud Shawky El Meteini, Marwa Matboli
Summary: This study identified a group of differentially expressed mRNAs related to the cGAS/STING, NOD-like receptor pathways (NLR), and T2DM using a microarray dataset. Comparative analysis using real-time PCR revealed significantly higher expressions of ZBP1, DDX58, NFKB1, and CHUK in the prediabetes group compared to healthy controls or T2DM patients. The expressions of ZBP1 and NFKB1 mRNA could discriminate between good and poor glycemic control groups.
Article
Multidisciplinary Sciences
Noha Salah, Sanaa Eissa, Amal Mansour, Nagwa M. Abo El Magd, Amany Helmy Hasanin, Manal M. El Mahdy, Mohamed Kamel Hassan, Marwa Matboli
Summary: Non-alcoholic steatohepatitis (NASH), the aggressive variant of non-alcoholic fatty liver disease, can be regulated through the Hippo pathway. Probiotics, such as kefir milk, have been shown to effectively manage NASH. In this study, the efficacy of kefir milk in NASH management was investigated through the modulation of hepatic mRNA-miRNA panel linked to NAFLD/NASH Hippo signaling and gut microbita regulated genes. The results showed that kefir administration prevented steatosis and inflammatory component of NASH, improved liver function and lipid panel, and down-regulated the expression of miR 6807-5p while up-regulating the expression of SOX 11 and AMOTL2, resulting in the reduction of inflammatory and fibrotic markers. Overall, kefir suppressed NASH progression and improved fibrosis and hepatic inflammation.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Shaimaa Zabady, Nievin Mahran, Mohamed A. Soltan, Muhammad Alaa Eldeen, Refaat A. Eid, Sarah Albogami, Eman Fayad, Marwa Matboli, Eman K. Habib, Amany H. Hasanin, Mahmoud A. Ali, Noha M. Mesbah, Dina M. Abo-Elmatty, Asmaa R. Abdel-Hamed
Summary: C3G extracted from Morus alba L. has potential protective effects against hepatocellular carcinoma (HCC) by regulating the expression of autophagy protein ATG16L1 and related RNA molecules. This study provides evidence for the potential prophylactic use of C3G as a drug for HCC.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
