Article
Biotechnology & Applied Microbiology
Florence M. Schempp, Ingmar Strobel, Maria M. W. Etschmann, Elena Bierwirth, Johannes Panten, Hendrik Schewe, Jens Schrader, Markus Buchhaupt
Summary: Research has identified limonene-3-hydroxylase enzymes from two fungi, crucial for the production of (2)-menthol from renewable (+)-limonene in a two-step synthesis process. This study contributes to the development of a biotechnological process for (2)-menthol production utilizing renewable resources.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mohamed M. Radwan, Suman Chandra, Shahbaz Gul, Mahmoud A. ElSohly
Summary: Cannabis sativa, one of the oldest medicinal plants in the world, contains a complex mixture of secondary metabolites, with cannabinoids and non-cannabinoid constituents being the main components. This review focuses on the chemistry of cannabinoids and major non-cannabinoid constituents, discussing their chemical structures, methods of isolation, and identification.
Review
Chemistry, Organic
Archana Singh, Keisham S. Singh
Summary: Specialized metabolites produced by organisms in polar regions have applications in the pharmaceutical, food, and cosmetic industries. With climate change and resource exploration, it is important to understand the potential and scope of natural products in these regions.
CURRENT ORGANIC CHEMISTRY
(2022)
Review
Immunology
Xueling Liu, Zhiguo Wang, Hua Qian, Wenhua Tao, Ying Zhang, Chunyan Hu, Weiwei Mao, Qi Guo
Summary: Rheumatoid arthritis is an autoimmune disease affecting joints, and natural medicines have shown potential therapeutic effects on RA through mechanisms such as anti-inflammation, anti-oxidation, and immune regulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Nicole M. Desmet, Kalyani Dhusia, Wenjie Qi, Andrea I. Doseff, Sudin Bhattacharya, Assaf A. Gilad
Summary: Synthetic Biology has emerged as a new discipline in recent years, where cellular devices replace functions traditionally performed by electronic devices. A major challenge in the field is the crosstalk between cellular signaling pathways, but utilizing specific promoters and ligands has led to the successful creation of a synthetic gene off switch.
Review
Clinical Neurology
Chandra Kanta Bhusal, Daniel Ejim Uti, Dattatreya Mukherjee, Taha Alqahtani, Saud Alqahtani, Arghya Bhattacharya, Shopnil Akash
Summary: Parkinson's disease is a common neurodegenerative disorder characterized by degeneration of dopaminergic neurons. Natural phytocompounds have shown potential in managing PD, with their neuroprotective properties and ability to counteract neurodegenerative processes.
PARKINSONISM & RELATED DISORDERS
(2023)
Review
Chemistry, Medicinal
Francesco Melfi, Simone Carradori, Noemi Mencarelli, Cristina Campestre, Marialucia Gallorini, Silvia Di Giacomo, Antonella Di Sotto
Summary: This article provides a brief description of the anticancer potential of natural compounds, followed by a search for patents published from 2019 onwards according to the PRISMA guidelines. Additionally, relevant publications from the overall scientific literature were discussed.
EXPERT OPINION ON THERAPEUTIC PATENTS
(2023)
Article
Biochemistry & Molecular Biology
Qinghe Zhang, Fengbo Yang, Hong Tong, Yuan Hu, Xinyi Zhang, Tian Tian, Youjun Zhang, Qi Su
Summary: The ingestion of plant flavonoids enhances the tolerance of Bemisia tabaci to certain insecticides by increasing the activity of cytochrome P450 enzymes, which disrupts the mechanism of the insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Review
Medicine, Research & Experimental
Shi Wu, Yaobin Pang, Yingjie He, Xiaotong Zhang, Li Peng, Jing Guo, Jinhao Zeng
Summary: Atopic dermatitis (AD) presents a significant challenge for patients, and natural products such as puerarin, ferulic acid, and ginsenosides have shown promise in protecting against AD by inhibiting inflammatory cell activity. Future research should focus on developing natural products that address the clinical characteristics of AD for potential treatment options.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Andrew Yiakoumetti, Erik K. R. Hanko, Yutong Zou, Jeremy Chua, Jakub Chromy, Ruth A. Stoney, Kris Nino G. Valdehuesa, Jack A. Connolly, Cunyu Yan, Katherine A. Hollywood, Eriko Takano, Rainer Breitling
Summary: Flavones and flavonols are important classes of flavonoids. This study successfully produced various flavones and flavonols in E.coli using a rapid prototyping platform, with the highest titers reaching 128 mg L-1 and 151 mg L-1.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Aijun Wang, Li Ma, Xinyue Shu, Yuqi Jiang, Juan Liang, Aiping Zheng
Summary: CYP716A16 is a positive regulator of rice to Rhizoctonia solani AG1-IA and Xanthomonas oryzae pv. oryzae (Xoo), enhancing rice resistance through activating JA-dependent defense responses and increasing flavonoid contents in plants.
Article
Microbiology
Li Rong Lim, Garrett Wong, Maybelle K. K. Go, Wen Shan Yew
Summary: In this study, we demonstrated that Saccharomyces cerevisiae is a suitable host for the functional expression of the key enzyme CloA in the biosynthesis pathway of ergot alkaloids. Different orthologs of CloA were found to have varying abilities to oxidize the substrate agroclavine, with some orthologs only able to perform the first oxidation reaction. We also identified a region between the F-G helices of the enzyme that may play a role in substrate recognition and uptake. By engineering CloA, we achieved significantly higher production levels of lysergic acid, showing potential for industrial production of ergot alkaloids using biosynthetic routes.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Yongfa Wang, Hao Pan, Fuhao Wang, Chen Shen
Summary: This review focuses on the P450 groups in microorganisms, including their distribution and application. Microbial P450 enzymes have great potential for applications, but the functions of most of them have not been studied. Therefore, selecting suitable P450 enzymes from a large P450 library, combined with engineering and immobilization technologies, can promote the large-scale application of microbial P450 enzymes.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Microbiology
Xu Qian, Hui Jin, Zhuojun Chen, Qingqing Dai, Surendra Sarsaiya, Yitong Qin, Qi Jia, Leilei Jin, Jishuang Chen
Summary: A comparative transcriptome analysis was conducted on Trichoderma longibrachiatum strain MD33 and its positive mutant UN32, revealing differentially expressed genes that may impact sesquiterpene alkaloid production, potentially associated with the mevalonate (MVA) pathway. Differential expression of enzyme-coding genes suggests physical mutagenesis could disrupt gene expression.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yan Luo, Yaozhu Jiang, Linhao Chen, Chun Li, Ying Wang
Summary: This review summarizes the current progress in the studies of key enzymes (OSCs, P450s, and UGTs) in the triterpenoids synthetic pathway and discusses the application of protein engineering in the microbial synthesis of triterpenoids. The challenges of enzyme shortage, low expression, and low catalytic activity in microbes are addressed, and the potential of protein engineering in improving enzyme specificity, activity, and stability is highlighted.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marco Antonio Kevin Perez-Vazquez, Yesenia Pacheco-Hernandez, Edmundo Lozoya-Gloria, Clemente Mosso-Gonzalez, Sergio A. Ramirez-Garcia, Omar Romero-Arenas, Nemesio Villa-Ruano
Summary: The investigation demonstrates the antifungal activity of peppermint essential oil and its major volatiles against Fusarium sambucinum, delaying the onset of soft rot symptoms in cera peppers while maintaining fruit quality and nutritional content. These components also exert a preservative effect on carotenoids, ascorbic acid, and capsaicinoids in the fruits, making them a potential ecological alternative for postharvest control of fusariosis.
CHEMISTRY & BIODIVERSITY
(2022)
Letter
Surgery
Maria E. Aguilar-Aldrete, Jose Dominguez-Rodas, Carlos E. Cabrera-Pivaral, Nemesio Villa-Ruano, Sabina Lopez-Toledo, Nory O. Davalos-Rodriguez, Ana R. Rincon-Sanchez, Erick Ruiz-Balderas, Sergio A. Ramirez-Garcia
CIRUGIA Y CIRUJANOS
(2022)
Article
Food Science & Technology
Quetzali Nicte Morales-Rabanales, Wendy Abril Coyotl-Perez, Efrain Rubio-Rosas, Georgina Salud Cortes-Ramirez, Jose Francisco Sanchez Ramirez, Nemesio Villa-Ruano
Summary: This study reports on the use of hybrid films containing chitosan and leaf essential oil of Schinus molle to control postharvest diseases in tomatoes. The films showed strong antifungal properties and effectively delayed fungal growth while improving fruit firmness.
Article
Biochemistry & Molecular Biology
Yesenia Pacheco-Hernandez, Nemesio Villa-Ruano, Ramiro Cruz-Duran, Elvia Becerra-Martinez, Edmundo Lozoya-Gloria
Summary: The leaves of Piper auritum, known as 'Hoja Santa', have been consumed by native people of central and southern Mexico for centuries. Metabolomics profiling of different accessions of P.auritum harvested in different provinces of Mexico revealed that it is a rich source of essential amino acids, organic acids, monosaccharides, and valuable nutraceuticals. The analysis also showed that leaves harvested in Oaxaca have better nutritional properties, while those from the northern highlands of Puebla are safer for human consumption due to lower levels of the anti-nutrient safrole.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Nemesio Villa-Ruano, Nestor Hernandez-Silva, Jenaro Leocadio Varela-Caselis, Sergio Alberto-Ramirez-Garcia, Clemente Mosso-Gonzalez
Summary: In this study, a cell suspension system for the production of antioxidants carnosic acid (CA) and carnosol (CS) in Lepechinia meyenii was generated and evaluated through tissue culture approaches. External stimuli such as UV light exposure, polyethylene glycol, and NaCl were found to promote the maximum accumulation of CA and CS. Additionally, the bacterial elicitor pyocyanin was effective in increasing the production of both diterpenes. Metabolic engineering using Saccharomyces cerevisiae successfully led to the synthesis of miltiradiene/abietatriene. Optimized batch cultures with specific conditions resulted in a significant increase in the production of CA and CS.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Food Science & Technology
Cesar Omar Montoya-Garcia, Rosario Garcia-Mateos, J. Jesus Magdaleno-Villar, Victor Hugo Volke-Haller, Nemesio Villa-Ruano, L. Gerardo Zepeda-Vallejo, Elvia Becerra-Martinez
Summary: The aim of this study was to investigate the metabolomics profile of three native purslane cultivars from Mexico grown under hydroponic conditions using NMR spectroscopy. A total of 39 metabolites were identified in purslane, including sugars, amino acids, organic acids, and other compounds. Principal component analysis and orthogonal partial least squares discriminant analysis separated the cultivars into three clusters, with the Mixquic cultivar having the highest number of differential compounds. Additionally, changes in the metabolome were observed with later harvesting times for all cultivars.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Nemesio Villa-Ruano, Elvia Becerra-Martinez, Jose Maria Cunill-Flores, Jorge Ariel Torres-Castillo, Guillermo M. Horta-Valerdi, Yesenia Pacheco-Hernandez
Summary: Lepidopterism caused by caterpillar contact is a global public health problem. This study analyzes the venom of Hylesia continua larvae using H-1-Nuclear Magnetic Resonance (NMR) profiling. The venom contains 19 compounds with pain-inducing, inflammatory, and immunosuppressive activity. This is the first investigation to report such a complex mixture of small molecules with inflammatory properties in the venom of a lepidopteran larva.
Article
Plant Sciences
Antoni Garcia-Baeza, Miguel Angel Alcalde, Olivier Grovel, Isaias Balderas-Renteria, Nemesio Villa-Ruano, Manuel Velazquez-Ponce, Elvia Becerra-Martinez, Karla Ramirez-Estrada
Summary: English Summary: Hairy root cultures of Centella asiatica can be used as bioproduction platforms to produce high-valuable metabolites. The application of methyl-jasmonate and coronatine in these cultures can cause significant changes in the metabolism of the plants. In this study, the metabolic responses of hairy roots to these molecules were analyzed using metabolomics approaches. The results showed that coronatine had a greater effect on reprogramming the metabolome compared to methyl-jasmonate. The activation of specialized pathways and the consumption of carbohydrates and amino acids were observed in coronatine-treated cultures. These findings provide valuable insights into the metabolic alterations caused by methyl-jasmonate and coronatine and the potential to manipulate the metabolism of C. asiatica hairy roots.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Biochemistry & Molecular Biology
Yesenia Pacheco-Hernandez, Edmundo Lozoya-Gloria, Maricruz Rangel-Galvan, Jenaro Leocadio Varela-Caselis, Nemesio Villa-Ruano
Summary: This study found that the R. pompana anthocyanin fraction (RPAF) has inhibitory effects on alpha-glucosidase, pancreatic lipase, HMG-CoA reductase, and ornithine decarboxylase. It also regulates postprandial glucose levels in diabetic mice and reduces lipid levels.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Biochemistry & Molecular Biology
Yesenia Pacheco-Hernandez, Edmundo Lozoya-Gloria, Ramiro Cruz-Duran, Nemesio Villa-Ruano
Summary: We investigated the inhibitory properties of Solanum nigrescens anthocyanin fraction (SNAF) and its major constituents on different enzymes and their effects on triglyceride levels in mice. The fraction and major constituents showed strong inhibitory activity on pancreatic lipase and ameliorated hypertriglyceridemia. Furthermore, they had no inhibitory effect on beneficial bacteria but exhibited inhibitory activity on Helicobacter pylori ATCC 53504.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Pharmacology & Pharmacy
Nemesio Villa-Ruano, Maricruz Anaya-Ruiz, Luis Villafana-Diaz, Diana Barron-Villaverde, Martin Perez-Santos
Summary: Research shows that mito-Atovaquone can be used to treat various types of cancer, including pancreatic cancer, lung cancer, and brain cancer. The repurposing of this approved drug strategy saves time and investment.
PHARMACEUTICAL PATENT ANALYST
(2023)
Article
Horticulture
Yesenia Pacheco-Hernandez, Edmundo Lozoya-Gloria, Elvia Becerra-Martinez, Nemesio Villa-Ruano
Summary: This study elucidated the chemical and biochemical properties of several traditional foods from the northern highlands of Puebla city, revealing significant differences in their nutrient content, nutraceuticals, antioxidant capacity, and inhibitory properties against specific enzymes and microbial activity.
Article
Horticulture
Omar Romero-Arenas, Marco A. Kevin Perez-Vazquez, Antonio Rivera, Yesenia Pacheco-Hernandez, Sergio Alberto Ramirez-Garcia, Gerardo Landeta-Cortes, Nemesio Villa-Ruano
Summary: This study demonstrates the potential use of ZlEO and its major volatiles in preventing postharvest rot caused by Fusarium temperatum in manzano peppers. The results show that ZlEO and its major volatiles have fungicide activity against F. temperatum and can prolong the shelf life of infected peppers while preserving their nutritional value and quality.
