Article
Biochemical Research Methods
Hikmat Ghosson, Yann Guitton, Amani Ben Jrad, Chandrashekhar Patil, Delphine Raviglione, Marie-Virginie Salvia, Cedric Bertrand
Summary: It is crucial to consider ion suppression as a critical issue in metabolomics biomarker determination when comparing samples with heterogeneous metabolic profiles. Raw chromatograms and mass spectra crosschecks are necessary to identify and filter out potential false positives, and a dilution-based approach can help confirm reliable biomarker candidates before further analysis.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Review
Biochemistry & Molecular Biology
Rahul K. Suryawanshi, Raghuram Koganti, Alex Agelidis, Chandrashekhar D. Patil, Deepak Shukla
Summary: SARS-CoV-2 infection manipulates host pathways, reorganizes host cell structure, impairs innate cellular defenses, and induces delayed hyperinflammation while weakening interferon response. Understanding the complex interactions between SARS-CoV-2 and host signaling pathways provides crucial strategic clues for the development of novel therapeutics.
TRENDS IN MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Alex Agelidis, Benjamin A. Turturice, Rahul K. Suryawanshi, Tejabhiram Yadavalli, Dinesh Jaishankar, Joshua Ames, James Hopkins, Lulia Koujah, Chandrashekhar D. Patil, Satvik R. Hadigal, Evan J. Kyzar, Anaamika Campeau, Jacob M. Wozniak, David J. Gonzalez, Israel Vlodavsky, Jin-ping Li, David L. Perkins, Patricia W. Finn, Deepak Shukla
Summary: The drive to withstand environmental stresses and defend against invasion is a universal trait in all forms of life, but how signaling pathways interface to generate coordinated responses to diverse stimuli remains unclear. Research on heparanase reveals its role in disease development and its ability to counteract pathogen invasion through multiple defense mechanisms.
Article
Multidisciplinary Sciences
Alex Agelidis, Rahul K. Suryawanshi, Chandrashekhar D. Patil, Anaamika Campeau, David J. Gonzalez, Deepak Shukla
Summary: The study reveals the critical role of HPSE in cell cycle and stress responses, as well as its inhibition of innate responses to double-stranded DNA breakage. Modulating HPSE to control the DNA damage response offers a unique strategy in the development of unconventional multivalent therapy.
Article
Ophthalmology
Lulia Koujah, Mowafak Allaham, Chandrashekhar D. Patil, Joshua M. Ames, Rahul K. Suryawanshi, Tejabhiram Yadavalli, Alex Agelidis, Christine Mun, Bayasgalan Surenkhuu, Sandeep Jain, Deepak Shukla
Summary: This study investigated ocular pathologies associated with herpes simplex virus-1 infection, revealing genetic variances in clinical strains that may impact viral entry receptor usage and disease severity. Experimental approaches with patient ocular cultures and a murine model highlighted the potential role of genetic variances in dictating disease outcome and severity.
Article
Virology
Ghadah A. Karasneh, Divya Kapoor, Navya Bellamkonda, Chandrashekhar D. Patil, Deepak Shukla
Summary: The increased levels of HPSE can reduce syncytial plaque formation and promote viral egress and release. Transiently enhanced expression of HPSE does not affect HSV-1 entry into cells but facilitates extracellular release of mature virions.
Article
Multidisciplinary Sciences
Joshua Ames, Tejabhiram Yadavalli, Rahul Suryawanshi, James Hopkins, Alexander Agelidis, Chandrashekhar Patil, Brian Fredericks, Henry Tseng, Tibor Valyi-Nagy, Deepak Shukla
Summary: The study demonstrates that the autophagy receptor OPTN plays a crucial neuroprotective role during HSV-1 infection by degrading viral proteins, preventing virus spread and neuronal necroptosis. Lack of OPTN leads to cognitive decline and susceptibility to lethal CNS infection in mice, indicating its importance in survival from potentially deadly viral infections.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Rahul K. Suryawanshi, Chandrashekhar D. Patil, Raghuram Koganti, Sudhanshu Kumar Singh, Joshua M. Ames, Deepak Shukla
Summary: Research indicates that cationic peptides G1 and G2 may inhibit the entry of SARS-CoV-2 by reducing the essential receptor HS on cell surfaces and interfering with the virus interaction with ACE2.
Editorial Material
Cell Biology
Chandrashekhar D. Patil, Deepak Shukla
Summary: Very little is known about the mechanisms that restrict neurotropic herpesviruses from infecting the central nervous system and causing neuron death. However, recent research has shown that OPTN-mediated autophagy acts as an intrinsic immune barrier against these viruses and protects the CNS from neurodegenerative stress.
Article
Immunology
Chandrashekhar D. Patil, Rahul Suryawanshi, Joshua Ames, Raghuram Koganti, Alex Agelidis, Divya Kapoor, Tejabhiram Yadavalli, Lulia Koujah, Henry C. Tseng, Deepak Shukla
Summary: This study reveals the key role of optineurin (OPTN) in restricting HSV-2 infection and controlling viral spread. Lack of OPTN leads to enhanced virus production and impaired host autophagy response. The OPTN/CCL5 nexus identified in this study may represent an intrinsic host defense mechanism against herpesviruses.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Jitendra D. Salunkhe, Bhavana V. Mohite, Satish V. Patil
Summary: This study aimed to enhance the pharmacological activities of naringenin by modifying it with silver nanoparticles. The naringenin-nano silver conjugate showed significantly increased antibacterial potential against both Gram positive and Gram negative bacteria. It also exhibited improved antiacanthamoebal activity compared to naringin and standard naringenin. Therefore, modification of natural products as nanoconjugates is a promising approach to develop effective drugs.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Applied
Jitendra D. Salunkhe, Satish V. Patil
Summary: Naringinase is an important enzyme with both α-L-rhamnosidase and β-D-glucosidase activities. The traditional screening method has limitations and can result in false positive results. The double screen plate assay using synthetic substrates offers a more accurate way to identify genuine naringinase producers.
NATURAL PRODUCT RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Narendra S. Salunkhe, Sunil H. Koli, Bhavana Mohite, Vikas S. Patil, Satish Patil
Summary: This study reports an environment friendly, green approach for synthesizing silver nanoparticles using bacterial yellow color pigment. The nanoparticles showed potential in photo protection, antioxidant activity, and biocidal properties.
RESULTS IN CHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Pradnya B. Nikam, Jitendra D. Salunkhe, Tatiana Minkina, Vishnu D. Rajput, Beom Soo Kim, Satish V. Patil
Summary: Selenium nanoparticles (SeNPs) are essential micronutrients with significant applications in various fields. Biological synthesis is the preferred method for SeNPs synthesis due to its environmental safety, low toxicity, high stability, and low energy requirements. Many microorganisms have the ability to convert toxic selenium oxyanions into less toxic elemental Se nanoparticles, serving as detoxification agents.
RESULTS IN CHEMISTRY
(2022)
Letter
Materials Science, Multidisciplinary
Amir Nasajpour, Mohamadmahdi Samandari, Chandrashekhar D. Patil, Reza Abolhassani, Rahul K. Suryawanshi, Rainer Adelung, Horst-Gunter Rubahn, Ali Khademhosseini, Yogendra Kumar Mishra, Deepak Shukla, Ali Tamayol, Paul S. Weiss
Summary: The study developed and demonstrated a rose-thorn-inspired antiviral fibrous array that provides physical and chemical protection, disinfection, and sterilization through photocatalysis of hydrogen peroxide. Electron microscopy and X-ray photoelectron spectroscopy confirmed the morphology and chemical composition. The material showed dose-dependent antiviral properties against both HSV-1 and HSV-2.
ACS MATERIALS LETTERS
(2021)
