Review
Microbiology
Dan Zilberstein
Summary: Leishmania parasites reside in phagolysosomes of macrophages where they sense the lysosome-specific environment for differentiation initiation. Protein kinase A plays a crucial role as the gatekeeper in this process.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Dan Zilberstein, Peter J. Myler
Summary: Leishmania parasites cause human leishmaniasis and cycle between hosts, utilizing sensors to adjust arginine transporter levels in response to environmental arginine concentrations.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kendi Okuda, Miriam Maria Silva Costa Franco, Ari Yasunaga, Ricardo Gazzinelli, Michel Rabinovitch, Sara Cherry, Neal Silverman
Summary: Leishmania parasites utilize virulence mechanisms to invade and replicate in macrophages, inhibiting host defenses and altering gene expression to enhance their survival and replication.
Article
Immunology
Paula Diez, Martin Perez-Andres, Martin Bogsted, Mikel Azkargorta, Rodrigo Garcia-Valiente, Rosa M. Degano, Elena Blanco, Sheila Mateos-Gomez, Paloma Barcena, Santiago Santa Cruz, Rafael Gongora, Felix Elortza, Alicia Landeira-Vinuela, Pablo Juanes-Velasco, Victor Segura, Raul Manzano-Roman, Julia Almeida, Karen Dybkaer, Alberto Orfao, Manuel Fuentes
Summary: This study delves into the quantitative proteomic profiles of B-cells undergoing antigen-dependent maturation, revealing that the differences among B-cell subpopulations are a combination of stage-specific protein expression and changes in relative protein expression levels related to metabolic regulation. The considerable overlap in proteomic profiles from the 5 studied B-cell subsets underscores the key role of regulating the stoichiometry of molecules associated with metabolic regulation, programming, and other signaling cascades crucial for the transition between each B-cell maturation stage.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Microbiology
Eleanor C. Saunders, Marijke F. Sernee, Julie E. Ralton, Malcolm J. McConville
Summary: Leishmania parasites can survive long-term in the mature phagolysosome compartment of host cells by switching to a slow growth state and activating a stringent metabolic response. The heterogeneous expression of this response in vivo reflects temporal and spatial differences in lesion tissues, including dormant stages.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hanwei Jiao, Zhixiong Zhou, Bowen Li, Yu Xiao, Mengjuan Li, Hui Zeng, Xiaoyi Guo, Guojing Gu
Summary: Brucellosis, caused by Brucella, is a highly prevalent zoonotic disease with low mortality but harmful impact on human health and various industries. Brucella utilizes intracellular parasitism and immune evasion mechanisms to establish persistent infection, posing challenges to treatment and prevention efforts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lova Prasadareddy Kajuluri, Shivani Gargvanshi, Amogh A. Sahasrabuddhe
Summary: MyosinXXI can be used as a biomarker to detect the presence of L. seymouri in Leishmania cultures and quantify its load. This method is a remarkable tool for identifying laboratory strains of Leishmania.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Microbiology
Ramu Dandugudumula, Renana Fischer-Weinberger, Dan Zilberstein
Summary: In this study, the dynamics of microtubules during Leishmania development were investigated, revealing the importance of microtubule stabilization and rearrangement in parasite rounding and elongation processes.
Review
Immunology
Alessandro F. Valdez, Daniel Zamith Miranda, Allan Jefferson Guimaraes, Leonardo Nimrichter, Joshua D. Nosanchuk
Summary: This review discusses the virulence factors and pathogenesis traits of Histoplasma capsulatum, a thermally dimorphic fungus causing histoplasmosis. Understanding these mechanisms is crucial for developing new preventive and therapeutic interventions.
Article
Multidisciplinary Sciences
Megan K. Fentress, Anthony W. De Tomaso
Summary: Cell competition is a universal quality control process that contributes to an individual's fitness. In the germline regeneration process of colonial ascidian Botryllus schlosseri, competition between germline stem cells (GSCs) determines the development of germ cells. Winners of the competition exhibit faster migration and larger clusters, which are correlated with the expression of the Notch ligand, Jagged. The homing ability and niche occupancy controlled by the differential utilization of the Notch pathway play a role in GSC competition.
Article
Parasitology
Viviane Noll Louzada-Flores, Maria Stefania Latrofa, Maria Stella Lucente, Bibiana Paula Dambros, Jairo Alfonso Mendoza-Roldan, Ilaria Varotto-Boccazzi, Giulia Maria Cattaneo, Gerald F. Spath, Alessio Buonavoglia, Domenico Otranto
Summary: This study aimed to evaluate the ability of non-pathogenic species Leishmania tarentolae to colonize, survive, and persist in canine primary monocyte-derived mononuclear cells. The results showed that L. tarentolae strains can infect and persist inside the mononuclear cells of dogs, indicating the potential for further laboratory studies.
Article
Immunology
Atieh Moradimotlagh, Stella Chen, Sara Koohbor, Kyung-Mee Moon, Leonard J. Foster, Neil Reiner, Devki Nandan
Summary: This study shows that Leishmania infection selectively increases the abundance of host Argonaute 1 in infected macrophages, leading to higher levels of active Ago1 complexes and promoting RNAi. Silencing Ago1 significantly reduces Leishmania intracellular survival, and the level of macrophage Ago1 is related to the expression of several Leishmania pathogenesis-related proteins.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Exequiel O. J. Porta, Jaime A. Isern, Karunakaran Kalesh, Patrick G. Steel
Summary: In this study, a new therapeutic target for the treatment of leishmaniasis was discovered using an activity-based protein profiling strategy to investigate serine proteases (SPs) in Leishmania parasites. Significant differences in the expression levels of active Leishmania serine hydrolases (SHs) were observed throughout the parasite's life cycle and between different species. Two targetable SPs were identified in Leishmania mexicana using quantitative proteomic mass spectrometry. The results of this study provide templates for the discovery of new drugs for the treatment of leishmaniasis.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Pharmacology & Pharmacy
Muhammad Ajmal Khan, Aftab Amin, Awais Farid, Amin Ullah, Abdul Waris, Khyber Shinwari, Yaseen Hussain, Khalaf F. Alsharif, Khalid J. J. Alzahrani, Haroon Khan
Summary: Infectious diseases caused by intracellular pathogenic bacteria (IPB) remain a significant global health issue. Conventional vaccination methods have made significant progress, but limitations still prevent the development of adequate vaccines for many IPB-related diseases. However, the advent of genomics technology has opened up new avenues for vaccine research, allowing researchers to utilize complete genomic data to identify potential vaccine candidates and predict their effectiveness.
Article
Multidisciplinary Sciences
Yosuke Harada, Tomohisa Sujino, Kentaro Miyamoto, Ena Nomura, Yusuke Yoshimatsu, Shun Tanemoto, Satoko Umeda, Keiko Ono, Yohei Mikami, Nobuhiro Nakamoto, Kaoru Takabayashi, Naoki Hosoe, Haruhiko Ogata, Tuneo Ikenoue, Atsushi Hirao, Yoshiaki Kubota, Takanori Kanai
Summary: This study revealed the metabolic properties of intestinal intraepithelial lymphocytes (IELs) and found that the intraepithelial compartment becomes hypoxic in the presence of microbes. Induced CD4(+)CD8 alpha alpha+TCR beta(+) T cells (DPIELs) increase in this location and have lower oxygen and glucose consumption, as well as unique alterations in mitochondria.
Editorial Material
Biochemical Research Methods
Angelo Facchiano, Claudia Angelini, Roberta Bosotti, Alessandro Guffanti, Anna Marabotti, Roberto Marangoni, Stefano Pascarella, Paolo Romano, Andreas Zanzoni, Manuela Helmer-Citterich
BMC BIOINFORMATICS
(2015)
Article
Biochemical Research Methods
Fabrizio Ferre, Alessio Colantoni, Manuela Helmer-Citterich
BRIEFINGS IN BIOINFORMATICS
(2016)
Article
Biochemistry & Molecular Biology
Pau Creixell, Erwin M. Schoof, Craig D. Simpson, James Longden, Chad J. Miller, Hua Jane Lou, Lara Perryman, Thomas R. Cox, Nevena Zivanovic, Antonio Palmeri, Agata Wesolowska-Andersen, Manuela Helmer-Citterich, Jesper Ferkinghoff-Borg, Hiroaki Itamochi, Bernd Bodenmiller, Janine T. Erler, Benjamin E. Turk, Rune Linding
Article
Cell Biology
Elena Morini, Barbara Rizzacasa, Sabina Pucci, Chiara Polidoro, Fabrizio Ferre, Daniela Caporossi, Manuela Helmer Citterich, Giuseppe Novelli, Francesca Amati
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2016)
Article
Biochemistry & Molecular Biology
Eugenio Mattei, Marco Pietrosanto, Fabrizio Ferre, Manuela Helmer-Citterich
NUCLEIC ACIDS RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Jon Ison, Kristoffer Rapacki, Herve Menager, Matus Kalas, Emil Rydza, Piotr Chmura, Christian Anthon, Niall Beard, Karel Berka, Dan Bolser, Tim Booth, Anthony Bretaudeau, Jan Brezovsky, Rita Casadio, Gianni Cesareni, Frederik Coppens, Michael Cornell, Gianmauro Cuccuru, Kristian Davidsen, Gianluca Della Vedova, Tunca Dogan, Olivia Doppelt-Azeroual, Laura Emery, Elisabeth Gasteiger, Thomas Gatter, Tatyana Goldberg, Marie Grosjean, Bjoern Gruening, Manuela Helmer-Citterich, Hans Ienasescu, Vassilios Ioannidis, Martin Closter Jespersen, Rafael Jimenez, Nick Juty, Peter Juvan, Maximilian Koch, Camille Laibe, Jing-Woei Li, Luana Licata, Fabien Mareuil, Ivan Micetic, Rune Mollegaard Friborg, Sebastien Moretti, Chris Morris, Steffen Moeller, Aleksandra Nenadic, Hedi Peterson, Giuseppe Profiti, Peter Rice, Paolo Romano, Paola Roncaglia, Rabie Saidi, Andrea Schafferhans, Veit Schwammle, Callum Smith, Maria Maddalena Sperotto, Heinz Stockinger, Radka Svobodova Varekova, Silvio C. E. Tosatto, Victor de la Torre, Paolo Uva, Allegra Via, Guy Yachdav, Federico Zambelli, Gert Vriend, Burkhard Rost, Helen Parkinson, Peter Longreen, Soren Brunak
NUCLEIC ACIDS RESEARCH
(2016)
Article
Dermatology
May El Hachem, Paola Fortugno, Antonio Palmeri, Manuela Helmer Citterich, Andrea Diociaiuti, Vittoria Proto, Renata Boldrini, Giovanna Zambruno, Daniele Castiglia
ACTA DERMATO-VENEREOLOGICA
(2016)
Article
Cardiac & Cardiovascular Systems
Ruggiero Mango, Andrea Luchetti, Raffaele Sangiuolo, Valentina Ferradini, Nicola Briglia, Emiliano Giardina, Fabrizio Ferre, Manuela Helmer Citterich, Francesco Romeo, Giuseppe Novelli, Federica Sangiuolo
CIRCULATION JOURNAL
(2016)
Article
Biochemistry & Molecular Biology
Marco Pietrosanto, Eugenio Mattei, Manuela Helmer-Citterich, Fabrizio Ferre
NUCLEIC ACIDS RESEARCH
(2016)
Article
Genetics & Heredity
Elisabetta Tabolacci, Giorgia Mancano, Stella Lanni, Federica Palumbo, Martina Goracci, Fabrizio Ferre, Manuela Helmer-Citterich, Giovanni Neri
EPIGENETICS & CHROMATIN
(2016)
Article
Oncology
Maria Patrizia Mongiardi, Mauro Savino, Maria Laura Falchetti, Barbara Illi, Francesca Bozzo, Cristiana Valle, Manuela Helmer-Citterich, Fabrizio Ferre, Sergio Nasi, Andrea Levi
Article
Biochemical Research Methods
Luca Parca, Bruno Ariano, Andrea Cabibbo, Marco Paoletti, Annalaura Tamburrini, Antonio Palmeri, Gabriele Ausiello, Manuela Helmer-Citterich
Article
Cell Biology
Valentina Cianfanelli, Claudia Fuoco, Mar Lorente, Maria Salazar, Fabio Quondamatteo, Pier Federico Gherardini, Daniela De Zio, Francesca Nazio, Manuela Antonioli, Melania D'Orazio, Tatjana Skobo, Matteo Bordi, Mikkel Rohde, Luisa Dalla Valle, Manuela Helmer-Citterich, Christine Gretzmeier, Joern Dengjel, Gian Maria Fimia, Mauro Piacentini, Sabrina Di Bartolomeo, Guillermo Velasco, Francesco Cecconi
NATURE CELL BIOLOGY
(2015)
Review
Genetics & Heredity
Antonio Palmeri, Fabrizio Ferre, Manuela Helmer-Citterich
FRONTIERS IN GENETICS
(2014)
Article
Biochemical Research Methods
Marco Pietrosanto, Marta Adinolfi, Riccardo Casula, Gabriele Ausiello, Fabrizio Ferre, Manuela Helmer-Citterich