Article
Pharmacology & Pharmacy
Eriko Yamazoe, Jia-You Fang, Kohei Tahara
Summary: Densely polyethylene glycol-modified liposomes showed mucus permeability and improved oral absorption compared to mucoadhesive liposomes. The in vivo and in vitro experiments revealed that modifying liposomes with PEG increased oral absorption but excessive PEGylation decreased absorption. Incorporating spermine as an absorption enhancer in PEGylated liposomes showed the highest in vivo absorption of the model peptide drug tested.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Eliete de Souza Von Zuben, Josimar Oliveira Eloy, Maiara Destro Inacio, Victor Hugo Sousa Araujo, Amanda Martins Baviera, Maria Palmira Daflon Gremiao, Marlus Chorilli
Summary: Liposomes functionalized with cell-penetrating peptides are a promising strategy for delivering insulin through the nasal route. Hydrogel-based carriers made of hydroxyethylcellulose (HEC) possess desirable rheological properties and mucoadhesion for nasal administration. The incorporation of liposomes can enhance the drug retention, while the functionalization with peptides can affect the release rate of insulin.
Article
Biochemistry & Molecular Biology
Nan Zhang, Tao Bing, Luyao Shen, Le Feng, Xiangjun Liu, Dihua Shangguan
Summary: A high-affinity DNA aptamer (HG1-9) targeting human tumor cells was developed through cell-SELEX, showing potential as a promising ligand for general cancer-targeted diagnostics and therapeutics. The aptamer was found to bind to the human transferrin receptor and cross the epithelium barrier through transferrin receptor-mediated transcytosis for targeted drug delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biophysics
Diego Salas Sanzana, Emilia Flores Faundez, Jaime Melendez, Marco Soto-Arriaza
Summary: The functionalization of liposomes with snake venom phospholipase A2 peptides has been found to enhance the encapsulation and cytotoxicity of doxorubicin, leading to improved antitumor efficacy in HeLa cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Chaemin Lim, Yuseon Shin, Kioh Kang, Patihul Husni, Dayoon Lee, Sehwa Lee, Han-Gon Choi, Eun Seong Lee, Yu Seok Youn, Kyung Taek Oh
Summary: Previous studies have shown that increasing the length of the PEG linker can enhance the tumor-targeting ability of liposomes in vivo, leading to increased antitumor activity of the encapsulated drug.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Anselm F. L. Schneider, Marina Kithil, M. Cristina Cardoso, Martin Lehmann, Christian P. R. Hackenberger
Summary: The challenge of delivering proteins into cells has been addressed by conjugating arginine-rich cell-penetrating peptides to the cell surface, enabling efficient delivery of protein-CPP conjugates. The use of thiol-reactive cell-penetrating peptides allows for simple co-incubation to deliver thiol-containing proteins, greatly simplifying protein and antibody delivery with minimal genetic or chemical engineering.
Article
Pharmacology & Pharmacy
Stephanie Andrade, Joana A. Loureiro, Maria C. Pereira
Summary: This study prepared stealth liposomes for the delivery of gallic acid (GA) to the brain for Alzheimer's disease (AD) therapy. The liposomes were stable and efficiently released GA, and showed strong interaction with amyloid beta (A beta), slowing down its aggregation and disaggregating preformed fibrils.
Article
Medicine, Research & Experimental
Haruya Yagi, Takumi Tomono, Yuma Handa, Natsuki Saito, Masami Ukawa, Kohei Miyata, Koichi Shigeno, Shinji Sakuma
Summary: This study investigates the use of cell-penetrating peptides anchored to polymeric platforms as an absorption enhancer for delivering biologics via mucosal routes. Results show that hyaluronic acid modified with L-octaarginine significantly enhances the mucosal absorption of protein drugs in the nasal cavities. The lung mucosa and oral mucosa were found to be suitable routes for mucosal absorption of biologics.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Kai-Hsuan Hsieh, Chih-Ying Hsu, I-Ju Hung, Chih-Ling Yeh, Yau-Hung Chen, Chien-Chung Cheng
Summary: The study demonstrates that liposomes composed of rho-KTTKS-CONH2 have higher toxicity due to better transport across the cell membrane, increasing the effective concentration inside cells. Staining of zebrafish embryos using rho-KTTKS-CONH2 liposomes shows a longer retention time, suggesting deeper tissue penetration.
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
(2021)
Review
Infectious Diseases
Gabriela Silva Cruz, Ariane Teixeira dos Santos, Erika Helena Salles de Brito, Gandhi Radis-Baptista
Summary: This review presents the antibacterial, antiparasitic, and antiviral effects of cell-penetrating antimicrobial peptides and highlights their potential for treating intracellular infections.
Article
Biophysics
Tianzhi Yang, Skye Curtis, Albert Bai, Abby Young, Derek Derosier, Shannon Ripley, Shuhua Bai
Summary: This study aimed to knock down P-glycoprotein (P-gp) expression through CRISPR-Cas9 gene-editing and establish a viable cell line for evaluating the pharmacoresistance of therapeutic agents mediated by efflux. A cationic liposome was prepared as a smart bomb by conjugating with a peptide-based targeting ligand that specifically bound to transferrin receptors at the blood-brain barrier (BBB), and then formed a nanocomplex with the P-gp knockdown CRISPR/Cas9 plasmid. The cell-friendly CRISPR/Cas9 targeted liposomes successfully knocked down the P-gp transporters and increased P-gp associated ATP activities in the transfected cells. Functional studies of the knocked down cells showed enhanced accumulation of rhodamine 123 and increased cytotoxic sensitivity to doxorubicin.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Jenny Jin, Kenji Schorpp, Daniel Samaga, Kristian Unger, Kamyar Hadian, Brent R. Stockwell
Summary: A machine learning approach combined with staining techniques was developed to unbiasedly classify different cell death mechanisms based on specific cell features, providing a novel analytic framework.
ACS CHEMICAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Siying Li, Ruixia Wang, Junfeng Li, Yulin Liu, Yanfeng Fu, Jing Zhou, Guocheng Yang, Yuping Shan
Summary: The peptide HAIYPRH (T7) is commonly used as a ligand in tumor-targeted nanodrug delivery systems. This study used atomic force microscopy to track the dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering cells, revealing that transferrin can both decrease endocytosis force and increase endocytosis speed depending on the cell type. This research provides important insights for the redesign and development of T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.
MOLECULAR PHARMACEUTICS
(2021)
Article
Engineering, Biomedical
Li Li, Anqi Chen, Bingmi Liu, Hao Pan, Yanjie Yu, Yu Liu
Summary: The article presents a modified liver delivery system for the anti-tumor drug pingyangmycin (PYM) using a hepatocellular carcinoma cell surface-specific ligand glycyrrhetinic acid (GA) and cell-penetrating peptide (TAT). The modified liposome, GA-TAT-PYM-L, showed stronger inhibitory and apoptosis promotion effects on HepG2 cells compared to PYM. Pharmacokinetic studies also showed enhanced mean residence time (MRT0-infinity) and area under curve (AUC(0-infinity)) for GA-TAT-PYM-L, indicating its liver targeting ability.
BIOMEDICAL MATERIALS
(2022)
Article
Pharmacology & Pharmacy
Dina V. Hingorani, Maria F. Camargo, Maryam A. Quraishi, Stephen R. Adams, Sunil J. Advani
Summary: Recent advances in immunotherapy have shown efficacy in cancer treatment by targeting immune checkpoint inhibitors and toll-like receptor agonists. A targeted peptide-drug conjugate strategy has been developed for precise drug delivery to tumor tissues, avoiding toxicity to normal tissues. This precision peptide-drug conjugate delivery highlights the potential for effective cancer therapy.
