Journal
MOLECULAR PHARMACEUTICS
Volume 18, Issue 3, Pages 1480-1485Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.molpharmaceut.0c01119
Keywords
transferrin; transferrin receptor; peptide-conjugated nanostructures; force tracing; atomic force microscopy
Funding
- National Natural Science Foundation of China [21773017]
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The peptide HAIYPRH (T7) is commonly used as a ligand in tumor-targeted nanodrug delivery systems. This study used atomic force microscopy to track the dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering cells, revealing that transferrin can both decrease endocytosis force and increase endocytosis speed depending on the cell type. This research provides important insights for the redesign and development of T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.
The HAIYPRH (T7) peptide has been widely used as a ligand for constructing tumor-targeted nanodrug delivery systems since it can target the transferrin receptor (TfR) and then enter cells easily with the help of transferrin (Tf). However, the dynamic mechanism by which transferrin promotes the entry of T7-conjugated nanostructures into cells remains unclear. Herein, a force tracing technique based on atomic force microscopy (AFM) was used to track the ultrafast dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering a cell at the single-particle level in real time. Tf helped decrease the endocytosis force and increase the endocytosis speed of AuNP-T7 in A549 cells. However, Tf only increased the endocytosis speed of AuNP-T7 in HeLa cells. In contrast, in Vero cells without TfR overexpression, Tf decreased the endocytosis speed. This report provides important insights for redesigning and developing T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.
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