Review
Biochemistry & Molecular Biology
Noora Barzkar, Zahoor Khan, Saeid Tamadoni Jahromi, Sajjad Pourmozaffar, Mohsen Gozari, Reza Nahavandi
Summary: Marine organisms are valuable sources of enzymes and their inhibitors with great therapeutic potential, particularly serine proteases and their inhibitors showing promise in biomedical applications. The uncontrolled activity of these proteases can lead to diseases, but their actions are regulated by serine protease inhibitors to maintain physiological functions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Shanshan Qin, Zidi Wang, Congcong Huang, Pan Huang, Dandan Li
Summary: In this study, it was found that the serine protease PRSS23 promotes tumor-associated macrophage infiltration in gastric cancer by regulating the expression and secretion of FGF2, resulting in a poor prognosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Medicine, Research & Experimental
E. Burchacka, P. Pieta, A. Lupicka-Slowik
Summary: There are four types of antifungal drugs targeting different aspects of fungi, but increasing resistance poses a challenge to treatment options. Thus, developing new drug strategies is crucial for addressing fungal diseases effectively.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Anwesha Sarkar, K. V. Bhaskara Rao
Summary: The study aimed to isolate a serine protease inhibitor compound with potential anticancer activity against colorectal and breast cancer cells from marine yeast. The yeast isolate ABS1 showed the highest inhibition activity of 89% against trypsin. The protease inhibitor protein from ABS1 was successfully purified and characterized.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dandan Li, Lingyun Xia, Pan Huang, Zidi Wang, Qiwei Guo, Congcong Huang, Weidong Leng, Shanshan Qin
Summary: In this study, PRSS56, a testis-specific serine protease, was identified as a novel cancer/testis antigen. PRSS56 was frequently overexpressed in various cancers, especially in gastrointestinal cancer. The expression of PRSS56 was associated with DNA methylation levels, and its decreased methylation led to overexpression in GC and CRC. Furthermore, PRSS56 activated the oncogenic PI3K/AKT signaling pathway in GC and CRC.
CELL AND BIOSCIENCE
(2023)
Article
Immunology
Xin Zhang, Miao Li, Layla El Moussawi, Sally Saab, Shasha Zhang, Mike A. Osta, Kristin Michel
Summary: Humoral immune responses in animals are tightly controlled by regulated proteolysis, where extracellular protease cascades activate key immune proteins and enzymes. In mosquitoes, the protease network regulates melanization reactions and affects resistance against microorganisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Jimin Hwang, Natalie Strange, Rami Mazraani, Matthew J. Phillips, Allan B. Gamble, Wilhelmina M. Huston, Joel D. A. Tyndall
Summary: A new series of CtHtrA inhibitors were developed by proline ring expansion and Cg-substitutions, which showed significantly improved antichlamydial activity in vitro.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Gillian A. Kelly-Robinson, James A. Reihill, Fionnuala T. Lundy, Lorcan P. McGarvey, John C. Lockhart, Gary J. Litherland, Keith D. Thornbury, S. Lorraine Martin
Summary: COPD is a complex disease characterized by unregulated proteolytic destruction of lung tissue mediated via a protease-antiprotease imbalance. Serpins, including AAT, play a crucial role in regulating protease activity in the lungs and understanding their physiological/pathological role could lead to new therapeutic opportunities for COPD and other chronic lung diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Tahsin F. Kellici, Ewa S. Pilka, Michael J. Bodkin
Summary: Serpins, a large and diverse family of proteins, play a crucial role in regulating inflammation, coagulation, thrombosis, thrombolysis, and immune responses. Recent advances in research on small-molecule modulators of serpins, their mode of action, and potential therapeutic perspectives are discussed, highlighting the importance of continued research in this field.
DRUG DISCOVERY TODAY
(2021)
Article
Chemistry, Medicinal
Patrick Gonschorek, Alessandro Zorzi, Tamara Maric, Mathilde Le Jeune, Mischa Schuettel, Mathilde Montagnon, Rebeca Gomez-Ojea, Denis Patrick Vollmar, Chantal Whitfield, Luc Reymond, Vanessa Carle, Hitesh Verma, Oliver Schilling, Alain Hovnanian, Christian Heinis
Summary: In this study, selective and high-affinity inhibitors of KLK5 and KLK7 were identified using phage display. The stability and half-life of the inhibitors were improved by eliminating proteaseprone sites and conjugating them to an albumin-binding peptide. These inhibitors were shown to reach the target KLKs in mouse epidermis and have the potential to control deregulated protease activity in Netherton syndrome.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Xiaoliang Wang, Damjan Avsec, Ales Obreza, Shida Yousefi, Irena Mlinaric-Rascan, Hans-Uwe Simon
Summary: This study demonstrates that serine protease inhibitors can block necrosis triggered by CD44, preventing cell death in GM-CSF-primed neutrophils. The inhibitors inhibit the activation of MLKL, p38 MAPK, and PI3K. However, they have no effect on apoptosis mediated by the FAS receptor.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medicine, General & Internal
Marcelo B. Zani, Aquiles M. Sant'Ana, Rafael C. Tognato, Jair R. Chagas, Luciano Puzer
Summary: Human tissue Kallikrein-related peptidases (hKLKs) play important roles in skin desquamation and skin diseases by cleaving corneodesmosomes and activating the PAR2 pathway. Inhibition of hKLKs shows promise as a therapeutic approach for improving the quality of life in patients with skin diseases.
FRONTIERS IN MEDICINE
(2022)
Article
Agronomy
Qijuan Gu, Zhiwei Wu, Yuenan Zhou, Zhizhi Wang, Jianhua Huang, Min Shi, Xuexin Chen
Summary: The study revealed that a serpin (CvT-serpin15) secreted by teratocytes can interfere with the growth of Gram-positive bacteria Staphylococcus aureus, and increase the survival rate of Plutella xylostella infected by S. aureus. This suggests that CvT-serpin15 plays a role in boosting the host immune system during parasitism, ultimately protecting the development of the wasp larva from bacterial infection.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Soohyung Lee, Inkyu Yoo, Yugyeong Cheon, Hakhyun Ka
Summary: This study investigates the expression, regulation, and function of PI3 and SLPI at the maternal-fetal interface in pigs. The results show that PI3 and SLPI are expressed in the endometrium and conceptus tissues of pigs, with higher levels during late pregnancy. In endometrial explant cultures, the expression of PI3 is induced by increasing doses of progesterone, while the expression of SLPI is induced by increasing doses of estradiol and progesterone.
Article
Immunology
Ruobing Guan, Shaoru Hu, Xiang Li, Shiheng An, Xuexia Miao, Haichao Li
Summary: This study focused on the transcriptome sequence of the Asian corn borer and identified several protease and protease inhibitor genes. One novel serine protease inhibitor gene, ACB-TIL, was found to be involved in the insect's immune response to Gram-negative bacteria and viruses. This gene has the potential to be a target for pest control.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Jiangrong Chen, Chunchao Zhu, Chaojie Wang, Chuansheng Hu, Daniel M. Czajkowsky, Yan Guo, Bingya Liu, Zhifeng Shao
JOURNAL OF MEDICAL GENETICS
(2020)
Article
Biochemistry & Molecular Biology
Quan Zhou, Xiongyan Wu, Xiaofeng Wang, Zhenjia Yu, Tao Pan, Zhen Li, Xinyu Chang, Zhijian Jin, Jianfang Li, Zhenggang Zhu, Bingya Liu, Liping Su
Correction
Oncology
Xiaofeng Wang, Guangtao Luo, Kundong Zhang, Jun Cao, Chen Huang, Tao Jiang, Bingya Liu, Liping Su, Zhengjun Qiu
Article
Oncology
Tao Pan, Zhijian Jin, Zhenjia Yu, Xiongyan Wu, Xinyu Chang, Zhiyuan Fan, Fangyuan Li, Xiaofeng Wang, Zhen Li, Quan Zhou, Jianfang Li, Bingya Liu, Liping Su
Article
Oncology
Qiang Zheng, Yan Huang, Xin Zeng, Xiaoyan Chen, Shihong Shao, Yan Jin, Qianqian Xue, Yue Wang, Yan Guo, Bin Gu, Chunyan Wu, Yuan Li
Summary: The study evaluated the clinicopathological and molecular profiles associated with PD-L1 expression in NSCLC in a large, multi-center Chinese cohort. PD-L1 expression was linked to histological type, pathological features, and driver mutation status, highlighting implications for clinical practice.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Tao Pan, Zhenjia Yu, Zhijian Jin, Xiongyan Wu, Airong Wu, Junyi Hou, Xinyu Chang, Zhiyuan Fan, Jianfang Li, Beiqin Yu, Fangyuan Li, Chao Yan, Zhongyin Yang, Zhenggang Zhu, Bingya Liu, Liping Su
Summary: The study revealed that lnc-CTSLP4 is significantly downregulated in gastric cancer tumor tissues, and by interacting with proteins and recruiting E3 ubiquitin ligase ZFP91, it inhibits the metastatic potential of GC cells.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Xiaoxia Guo, Fang Du, Qin Liu, Yan Guo, Qingbing Wang, Wei Huang, Zhongmin Wang, Xiaoyi Ding, Zhiyuan Wu
Summary: The study showed that IRE treatment increased the counts of WBC, neutrophils, and monocytes while decreasing lymphocyte count in patients. It also indicated a decrease in CD4(+) T cell subsets 1day post-IRE, with an increase in activated T cells and NK cells, and a decrease in Treg cells. Furthermore, there was a significant increase in cytotoxic CD8(+) T cells infiltration in ablative tumors in mice, along with elevated serum IFN-gamma levels in the IRE group.
Article
Oncology
Zhongyin Yang, Chao Yan, Wentao Liu, Wei Xu, Chen Li, Min Yan, Bingya Liu, Zhenggang Zhu
Summary: This study identified novel autoantibodies in the ascites of relapsed PTX-resistant gastric cancer patients, including TPM3, EFHD2, KRT19, and vimentin. These autoantibodies may serve as potential biomarkers for monitoring the occurrence of PTX-resistance.
Article
Biochemistry & Molecular Biology
Xufeng Wang, Xinyu Chang, Changyu He, Zhiyuan Fan, Zhenjia Yu, Beiqin Yu, Xiongyan Wu, Junyi Hou, Jianfang Li, Liping Su, Bingya Liu, Zhenggang Zhu
Summary: ATP5B is highly expressed in tumor tissues of GC and is positively correlated with age, tumor size, TNM stage, lymph node metastasis, and poor prognosis. Overexpression of ATP5B in GC cells increases cellular ATP content and promotes migration, invasion, and proliferation through the FAK/AKT/MMP2 pathway.
Article
Cell Biology
Zhenqiang Wang, Xinjing Wang, Tianqi Zhang, Liping Su, Bingya Liu, Zhenggang Zhu, Chen Li
Summary: MALAT1 promotes IL-6 secretion in GC cells by blocking autophagic flux, leading to cancer progression. Mechanistically, MALAT1 competitively interacts with ELAVL1 to stabilize PTEN mRNA and activate the AKT/mTOR pathway, impacting autophagic flux.
CELL DEATH & DISEASE
(2021)
Article
Medicine, General & Internal
Fangyuan Li, Jianfang Li, Junxian Yu, Tao Pan, Beiqin Yu, Qingqing Sang, Wentao Dai, Junyi Hou, Chao Yan, Mingde Zang, Zhenggang Zhu, Liping Su, Yuan-Yuan Li, Bingya Liu
Summary: The study successfully constructed a 9-gene GCP model for gastric cancer, identifying ARGLU1 as a potential therapeutic target that inhibits tumor growth by enhancing transcriptional levels of mismatch repair genes.
Article
Biochemistry & Molecular Biology
Zhijian Jin, Yifan Lu, Xiongyan Wu, Tao Pan, Zhenjia Yu, Junyi Hou, Airong Wu, Jianfang Li, Zhongyin Yang, Chen Li, Min Yan, Chao Yan, Zhenggang Zhu, Bingya Liu, Weihua Qiu, Liping Su
Summary: In this study, anlotinib inhibited gastric cancer (GC) cell growth by inducing apoptosis and G2/M phase arrest in a dose- and time-dependent manner. The interaction between epithelial and stromal cells in the tumor microenvironment affects the response of GC cells to anlotinib. Secreted lactate from GC cells activates cancer-associated fibroblasts (CAFs) to produce BDNF in a NF-kappa B-dependent manner, reducing the sensitivity of GC cells to anlotinib. Targeting the BDNF-TrkB pathway increased the sensitivity of GC cells to anlotinib in a human patient-derived organoid model.
Article
Biochemistry & Molecular Biology
Beiqin Yu, Wentao Dai, Li Pang, Qingqing Sang, Fangyuan Li, Junxian Yu, Haoran Feng, Jianfang Li, Junyi Hou, Chao Yan, Liping Su, Zhenggang Zhu, Yuan-Yuan Li, Bingya Liu
Summary: This study identified crucial transcription factors related to gastric cancer progression and proposed testable hypotheses on the differential regulation mechanisms of gastric carcinogenesis. Through cytological experiments and IHC staining, the dynamic changes of target genes regulated by one of the transcription factors were validated. The identified transcription factors and their targets may have potential therapeutic value.
MOLECULAR MEDICINE
(2022)
Article
Oncology
Zhongyin Yang, Chao Yan, Zhenjia Yu, Changyu He, Jianfang Li, Chen Li, Min Yan, Bingya Liu, Yingli Wu, Zhenggang Zhu
Summary: The study revealed that CDH11 is inhibited in relapsed PTX-resistant patients, and downregulated CDH11 expression promotes invasion, migration, and PTX resistance in GC cells. CDH11 may potentially serve as a predictive marker for the occurrence of PTX resistance in GC patients with peritoneal metastasis.
Article
Pharmacology & Pharmacy
Y. -N. Zhang, B. Liu, T. Jiang, Q. Li
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2020)