Article
Oncology
Xiajie Zhang, Kira Groen, Brianna C. Morten, Luiza Steffens Reinhardt, Hamish G. Campbell, Antony W. Braithwaite, Jean-Christophe Bourdon, Kelly A. Avery-Kiejda
Summary: This study aimed to evaluate the role of Delta 40p53 in breast cancer migration and invasion, finding an association between Delta 40p53 and gene expression profiles. Knockdown of both p53 alpha and Delta 40p53 resulted in increased proliferation, while overexpression of Delta 40p53 reduced proliferation rates and decreased migratory and invasive properties of the cells. These results suggest that at a basal level, Delta 40p53 works similarly to p53 alpha in suppressing cellular mobility and proliferation.
MOLECULAR ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Jing Zhao, Alan Blayney, Xiaorong Liu, Lauren Gandy, Weihua Jin, Lufeng Yan, Jeung-Hoi Ha, Ashley J. Canning, Michael Connelly, Chao Yang, Xinyue Liu, Yuanyuan Xiao, Michael S. Cosgrove, Sozanne R. Solmaz, Yingkai Zhang, David Ban, Jianhan Chen, Stewart N. Loh, Chunyu Wang
Summary: Epigallocatechin gallate (EGCG) in green tea induces apoptosis in cancerous cells through a direct interaction with the tumor suppressor p53, inhibiting p53 ubiquitination by its regulatory E3 ligase MDM2 and stabilizing p53 for anti-tumor activity.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Alexandra N. Boix De Jesus, Ahmad Taha, David Wang, Paulomi M. M. Mehta, Sunali Mehta, Ashley Reily-Bell, Sasini Polwatta Lekamlage, Adriana Machado Saraiva, Tahmeed Tahmeedzaman, Fouzia Ziad, Ziad Thotathil, Peter Y. C. Gan, Janice Royds, Antony Braithwaite, Noelyn Hung, Tania L. L. Slatter
Summary: The expression of delta 133p53 beta is increased in various primary tumors and is associated with increased proliferation, migration, and inflammation. In this study, it was found that delta 133p53 beta expression is also related to the development and survival of brain metastases. Furthermore, delta 133p53 beta-expressing cells have increased cancer-promoting properties and invasiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Kyra Nicole Laubach, Wensheng Yan, Xiangmudong Kong, Wenqiang Sun, Mingyi Chen, Jin Zhang, Xinbin Chen
Summary: p73 alpha 1 plays a critical role in tumor suppression and the inflammatory response via regulation of Notch1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Urology & Nephrology
Staffan Jahnson, Peter Soderkvist, Firas Aljabery, Hans Olsson
Summary: In stage T1 urinary bladder cancer, the combination of TERT and p53 mutations is associated with tumor progression. A possible protective effect of the TERT promoter mutation against tumor progression induced by the p53 mutation and subsequent p53 accumulation in tumor cells may exist, but further investigations are necessary.
Article
Biochemistry & Molecular Biology
Guanglin Niu, Isabel Hellmuth, Tatiana Flisikowska, Hubert Pausch, Beate Rieblinger, Alexander Carrapeiro, Benjamin Schade, Brigitte Boehm, Eva Kappe, Konrad Fischer, Bernhard Klinger, Katja Steiger, Reiner Burgkart, Jean-Christophe Bourdon, Dieter Saur, Alexander Kind, Angelika Schnieke, Krzysztof Flisikowski
Summary: The mutation in TP53 gene in pig models leads to the development of tumors like osteosarcoma, pointing out similarities with human TP53. Specific TP53 isoforms determine the tumor spectrum in pigs, with circTP53 playing a critical role in malignant transformation.
Article
Biochemistry & Molecular Biology
Sergio Hernandez, Francisca Alvarez-Astudillo, Daniel Garrido, Cristian Prieto, Alejandra Loyola, Rodrigo A. Villanueva
Summary: Research has shown that HBV X protein can generate three distinct isoforms that exhibit different subcellular localizations when expressed in cells. Among these, the XS isoform, which is small in size, displays a predominantly cytoplasmic localization. Additionally, XS plays a crucial role in HBV replication, and infection with HBV genotype H can impact the expression of the XS isoform.
Article
Biochemistry & Molecular Biology
Margit Kaldmae, Thibault Vosselman, Xueying Zhong, Dilraj Lama, Gefei Chen, Mihkel Saluri, Nina Kronqvist, Jia Wei Siau, Aik Seng Ng, Farid J. Ghadessy, Pierre Sabatier, Borivoj Vojtesek, Medoune Sarr, Cagla Sahin, Nicklas Osterlund, Leopold L. Ilag, Venla A. Vaananen, Saikiran Sedimbi, Marie Arsenian-Henriksson, Roman A. Zubarev, Lennart Nilsson, Philip J. B. Koeck, Anna Rising, Axel Abelein, Nicolas Fritz, Jan Johansson, David P. Lane, Michael Landreh
Summary: Disordered proteins pose challenges to structural biology. Researchers have found that fusing the protein with a highly soluble spider silk domain can modulate its characteristics and enhance translation efficiency. This mechanism is of great significance for cancer treatment.
Article
Biochemistry & Molecular Biology
Emma J. van Bodegraven, Jacqueline A. Sluijs, A. Katherine Tan, Pierre A. J. T. Robe, Elly M. Hol
Summary: GFAP is a highly expressed protein in astrocytes, neural stem cells, and gliomas, and is used as a diagnostic marker due to its differential expression between glioma subtypes. The discovery of a new GFAP isoform, GFAP mu, adds to the complexity of GFAP regulation and highlights the importance of studying IF splice variants for glioma research.
Article
Clinical Neurology
Shinichiro Yamada, Atsushi Hashizume, Yasuhiro Hijikata, Daisuke Ito, Yoshiyuki Kishimoto, Madoka Iida, Haruki Koike, Akihiro Hirakawa, Masahisa Katsuno
Summary: The study demonstrated that urinary N-terminal titin fragment could serve as a biomarker for ALS, related to disease prognosis, and could be used in disease monitoring and prediction of disease progression in combination with serum NfL.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2021)
Article
Biochemistry & Molecular Biology
Sven Giese, Theresia Reindl, Patrick Y. A. Reinke, Lilach Zattelman, Roman Fedorov, Arnon Henn, Manuel H. Taft, Dietmar J. Manstein
Summary: Myosin-1C is a single-headed, short-tailed member of the myosin class I subfamily. Alternative splicing of the MYO1C gene in vertebrates produces three isoforms with different N-terminal extensions. These extensions affect the chemomechanical coupling of the myosin-1C isoforms, leading to differences in power output, velocity, and resistive force. The N-terminal extensions reduce the length of the working stroke and the rate of ADP release, especially in myosin-1C35. The functional differences between isoforms are further amplified by the presence of external loads.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jing Zhao, Xinyue Liu, Alan Blayney, Yumeng Zhang, Lauren Gandy, Paige Olivia Mirsky, Nathan Smith, Fuming Zhang, Robert J. Linhardt, Jianhan Chen, Christopher Baines, Stewart N. Loh, Chunyu Wang
Summary: The mitochondrial permeability transition pore (mPTP) plays crucial roles in various diseases. Cyclophilin D is confirmed as the only component of mPTP. Previous studies have reported that p53 interacts with CypD through its DNA-binding domain (DBD). However, this study found that the N-terminal domain (NTD) of p53 is the major binding site for CypD. The study provides insights into the dynamic interface between NTD and CypD.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Meng-Han Huang, Jin-Tao Wang, Jian-Hui Zhang, Xue-Ling Mao, Gui-Xin Peng, Xiuying Lin, Daizhu Lv, Chen Yuan, Huan Lin, En-Duo Wang, Xiao-Long Zhou
Summary: This study reveals that METTL8 regulates m3C32 modification in mitochondria, with METTL8-Iso4 being unable to activate m3C32 formation due to the lack of an N-Extension. Additionally, it is found that METTL8-Iso1 efficiently introduces m3C32 into cytoplasmic or bacterial tRNAs in vitro and stimulates the activity of mitochondrial tRNA modification and charging.
Article
Health Care Sciences & Services
Alyssa Bowling, Alice Eastman, Christian Merlo, Gabrielle Lin, Natalie West, Shivani Patel, Garry Cutting, Neeraj Sharma
Summary: This study investigates N-terminal variants in the CFTR gene, finding that they can escape NMD and generate stable transcripts. By combining readthrough compounds and protein modulators, CFTR function can be restored.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Oncology
Firas Aljabery, Ivan Shabo, Aus Saudi, Martin Holmbom, Hans Olson, Staffan Jahnson
Summary: This study investigated the association between p53 expression in tumor cells and intratumoral macrophage infiltration in muscle-invasive urinary bladder cancer (MIBC), as well as their relationship with clinical and pathological variables and outcomes after radical cystectomy. The results showed that high CD163+ macrophage infiltration in the tumor microenvironment often coexisted with p53+ cancer cells, and this simultaneous expression was associated with improved CSS.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Anne Havermans, Charlotte G. G. M. Pauwels, Erna J. Z. Krusemann, Wouter F. Visser, Reinskje Talhout
Summary: The presence of nicotine salt-containing e-liquids in the Netherlands market has increased significantly in recent years. Nicotine salts, due to their characteristics, make these e-liquids more addictive and attractive, particularly to young and inexperienced users. Policymakers are advised to regulate products containing nicotine salts.
Article
Substance Abuse
Jeroen L. A. Pennings, Geoffrey Ferris Wayne, Walther N. M. Klerx, Charlotte G. G. M. Pauwels, Reinskje Talhout
Summary: This study explores the impact of tobacco blend and design characteristics on the sensory experience of cigarette consumers. The findings suggest that while some variations in design are noticeable to consumers, many products on the market are not discernibly different, indicating that brand diversity may serve non-sensory purposes such as marketing.
Article
Substance Abuse
Ingrid M. E. Bakker-'t Hart, Frank Bakker, Jeroen L. A. Pennings, Naomi Weibolt, Selma Eising, Reinskje Talhout
Summary: This study examines the commonly used flavors and flavorings in waterpipe tobacco and related products, and presents a waterpipe tobacco flavor wheel for experts' reference. The analysis reveals that the majority of waterpipe tobacco products are flavored, with fruit flavors being the most prevalent. Herbal molasses and steam stones contain similar levels of flavorings, with lower levels compared to waterpipe tobacco. The flavorings in waterpipe products are mainly fruity and sweet, often combined with menthol/mint flavorings. Banning flavorings in all waterpipe products could be an effective strategy to reduce waterpipe smoking among youth.
Article
Pharmacology & Pharmacy
Marja Driessen, Suzanne van der Plas-Duivesteijn, Anne S. Kienhuis, Evert-Jan van den Brandhof, Marianne Roodbergen, Bob van de Water, Herman P. Spaink, Magnus Palmblad, Leo T. M. van der Ven, Jeroen L. A. Pennings
Summary: The zebrafish embryo is a promising model for studying hepatotoxicity and could potentially reduce the use of rodents in such assessments. Proteomics analysis identified a set of potential protein markers for detecting adverse liver responses.
Article
Multidisciplinary Sciences
Andreja Novak, Jeroen L. A. Pennings, Larissa van der Maas, Hugo D. Meiring, Irene Ludwig, Saertje Verkoeijen, Victor Rutten, Femke Broere, Arjen Sloots
Summary: Stimulation of a canine monocyte/macrophage cell line with Leptospira strains and vaccines revealed differentially expressed genes and proteins, suggesting potential biomarkers for Leptospira vaccine quality control.
SCIENTIFIC REPORTS
(2022)
Review
Substance Abuse
Anette K. Bolling, Nadja Mallock, Efthimios Zervas, Stephanie Caille-Garnier, Thibault Mansuy, Cecile Michel, Jeroen L. A. Pennings, Thomas Schulz, Per E. Schwarze, Renata Solimini, Jean-Pol Tassin, Constantine Vardavas, Miguel Merino, Charlotte G. G. M. Pauwels, Lotte E. van Nierop, Claude Lambre, Anne Havermans
Summary: The study found significant limitations in the methodology used by international tobacco companies in their reports, rendering them unsuitable for determining whether priority additives should be banned in tobacco products according to the Tobacco Products Directive.
TOBACCO PREVENTION & CESSATION
(2022)
Review
Substance Abuse
Anne Havermans, Nadja Mallock, Efthimios Zervas, Thibault Mansuy, Jeroen L. A. Pennings, Thomas Schulz, Per E. Schwarze, Renata Solimini, Jean -Pol Tassin, Constantine I. Vardavas, Miguel Merino, Charlotte G. G. M. Pauwels, Lotte E. van Nierop, Claude Lambre, Anette K. Bolling
Summary: This study evaluates the 15 priority additives in the European Union Tobacco Products Directive. The industry reports were found to be insufficient and methodologically limited, and the issues of toxicity, addictiveness, and attractiveness of the additives were not adequately addressed. Alternative approaches such as the precautionary principle are recommended.
TOBACCO PREVENTION & CESSATION
(2022)
Article
Environmental Sciences
Victoria C. de Leeuw, Conny T. M. van Oostrom, Paul F. K. Wackers, Jeroen L. A. Pennings, Hennie M. Hodemaekers, Aldert H. Piersma, Ellen V. S. Hessel
Summary: There is an increasing recognition of the limitations of using animals for compound-induced developmental neurotoxicity (DNT) testing. Human stem cell-based models, such as the human neural progenitor test (hNPT), play a pivotal role in studying DNT as they can replicate relevant processes in human brain development. This study characterized the differentiation of neural progenitor cells over time and identified neurodevelopmental processes sensitive to compound exposure using transcriptomics.
Article
Reproductive Biology
Laura M. M. Samrani, Jeroen L. A. Pennings, Nina Hallmark, Remi Bars, Helen Tinwell, Marc Pallardy, Aldert H. Piersma
Summary: The study investigates the effect of exposure duration on gene expression changes in the all-trans retinoic acid (ATRA) signaling pathway in zebrafish embryotoxicity test (ZET). It is found that the highest magnitudes of gene expression regulation occur after 2-24 hrs exposure, with the optimal response at 4 hrs. Early assessment of gene expression regulation after exposure onset in ZET could be optimal for predicting developmental toxicity.
REPRODUCTIVE TOXICOLOGY
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Anne Havermans, Erna J. Z. Krusemann, Dion Zijtveld, Eva H. W. Huiberts, Peter M. J. Bos, Erna Schenk, Wouter F. Visser, Ingrid M. EBakker-'t Hart, Yvonne C. M. Staal, Reinskje Talhout
Summary: The Dutch government plans to only allow tobacco flavors in e-liquids to discourage e-cigarette use among youth. Researchers have compiled a restrictive list of 16 tobacco flavorings to implement this policy.
Article
Psychiatry
M. Liset Rietman, N. Charlotte Onland-Moret, Astrid C. J. Nooyens, Dorina Ibi, Ko Willems van Dijk, Leonard Daniel Samson, Jeroen L. A. Pennings, Maarten Schipper, Albert Wong, Annemieke M. W. Spijkerman, Martijn E. T. Dolle, W. M. Monique Verschuren
Summary: Cognitive decline is a normal part of aging, but factors such as genetics and environment can cause some individuals to experience a faster decline. This study suggests that the decline in overall cognitive function is influenced by different mechanisms than those involved in regulating cognitive function.
TRANSLATIONAL PSYCHIATRY
(2022)
Review
Chemistry, Medicinal
Christina H. J. Veltman, Jeroen L. A. Pennings, Bob van de Water, Mirjam Luijten
Summary: Nongenotoxic (NGTX) carcinogens induce cancer through mechanisms other than direct DNA damage, primarily involving the induction of oxidative stress leading to regenerative proliferation. However, current carcinogenicity assessment methods mainly focus on genetic toxicity, which may overlook NGTX carcinogens. To improve the predictivity of carcinogenicity assessment, a mechanism-based approach such as the adverse outcome pathway (AOP) network for chemically induced oxidative stress leading to (NGTX) carcinogenesis is proposed. This AOP network considers the role of oxidative stress in cancer development and explores the mechanisms and biological effects of chemical-induced oxidative damage. Developing AOP networks relevant to human carcinogenesis will facilitate a transition to a mechanism-based, human-relevant carcinogenicity assessment with reduced reliance on laboratory animals.
CHEMICAL RESEARCH IN TOXICOLOGY
(2023)
Article
Neurosciences
Faycal Guedj, Elise Kane, Lauren A. Bishop, Jeroen L. A. Pennings, Yann Herault, Diana W. Bianchi
Summary: The effectiveness of the Ts65Dn mouse model in improving neurocognition in Down syndrome has not been successfully translated to humans. The Ts66Yah mouse model, which carries an extra chromosome and identical segmental trisomy as Ts65Dn, was used to investigate the underlying factors.
BIOLOGICAL PSYCHIATRY
(2023)
Article
Substance Abuse
Charlotte G. G. M. Pauwels, Wouter F. Visser, Jeroen L. A. Pennings, Ernesto P. Baloe, Arnout P. T. Hartendorp, Loes van Tiel, Mirjam van Mourik, Wim Vaessen, Sanne Boesveldt, Reinskje Talhout
Summary: This study aimed to determine whether nicotine salts increase sensory appeal at lower concentrations and compared the inhalation intensity of both types of e-liquids. The results showed that nicotine salts did not significantly affect sensory appeal, harshness, and puffing intensity compared to free-based nicotine.
DRUG AND ALCOHOL DEPENDENCE
(2023)
Article
Substance Abuse
Jeroen L. A. Pennings, Charlotte G. G. M. Pauwels, Erna Schenk, Reinskje Talhout
Summary: Cigarillos have emerged as a substitute for flavored cigarettes, which are prohibited in the European Union, and they are also cheaper. To gain a better understanding of the cigarillo market, we analyzed product data from manufacturers via the EU-CEG system.
TOBACCO INDUCED DISEASES
(2023)