Article
Oncology
Kai Guo, Di Qi, Bo Huang
Summary: The study revealed that lncRNA MEG8 plays a critical role in the development of NSCLC by modulating the miR-15a/b-5p/PSAT1 axis, indicating that lncRNA MEG8, miR-15a-5p, miR-15b-5p, and PSAT1 may serve as potential targets for NSCLC therapy.
CANCER CELL INTERNATIONAL
(2021)
Article
Cardiac & Cardiovascular Systems
My-Anh Nguyen, Huy-Dung Hoang, Adil Rasheed, Anne-Claire Duchez, Hailey Wyatt, Mary Lynn Cottee, Tyson E. Graber, Leah Susser, Sabrina Robichaud, Ibrahim Berber, Michele Geoffrion, Mireille Ouimet, Hilal Kazan, Lars Maegdefessel, Erin E. Mulvihill, Tommy Alain, Katey J. Rayner
Summary: miR-223 acts as a key regulator in macrophages, protecting against atherosclerosis by modulating cholesterol metabolism and inflammation pathways.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Tom Dendooven, Elisabeth Sonnleitner, Udo Blaesi, Ben F. Luisi
Summary: The bacterial RNA chaperone Hfq and its partner protein Crc form higher order assemblies on control regions of different P. aeruginosa target mRNAs. These assemblies have mRNA-specific quaternary architectures due to the combination of multivalent protein-protein interfaces and recognition of patterns in the RNA sequence. The structural polymorphism of these ribonucleoprotein assemblies enables selective translational repression of many different target mRNAs.
Article
Biochemistry & Molecular Biology
Emeline Bollaert, Melissa Claus, Virginie Vandewalle, Sandrine Lenglez, Ahmed Essaghir, Jean-Baptiste Demoulin, Violaine Havelange
Summary: The study found that miR-15a-5p induces chemoresistance in AML cells by inhibiting daunorubicin-induced autophagy, suggesting that this miRNA could be a promising therapeutic target for chemoresistant AML patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yoshitaka Matsuo, Toshifumi Inada
Summary: Genetic mutations, mRNA processing errors, and lack of charged tRNAs can slow down or inhibit translating ribosomes. Monitoring systems are in place to detect and dispose of incomplete nascent chains and aberrant translation products to prevent accumulation and maintain protein homeostasis. Yeast models have been instrumental in uncovering the molecular mechanisms of these co-translational quality control systems.
Article
Multidisciplinary Sciences
Dan He, Zhong-bao Ruan, Gui-xian Song, Ge-cai Chen, Fei Wang, Mei-xiang Wang, Mao-kun Yuan, Li Zhu
Summary: The study found that miR-15a-5p, TGF-beta 1, and collagen I were up-regulated while Smad7 was down-regulated in AF tissues and LPS-stimulated cells. MiR-15a-5p can regulate Smad7 expression and affect fibrosis and cell proliferation. These findings suggest that the miR-15a-5p/Smad7 pathway could be a potential target for AF therapy.
Article
Immunology
Katharina Hutter, Thomas Rulicke, Tamas G. Szabo, Lill Andersen, Andreas Villunger, Sebastian Herzog
Summary: MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in the development and function of immune cells. The miR-15 family has been shown to suppress tumors in chronic lymphocytic leukemia. However, its physiological functions are not well understood. This study reveals that the miR-15 family restricts the expansion of progenitor B cells by inhibiting cell cycle regulators and IL-7 receptor expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Paula Gonzalez-Lopez, Marta Alvarez-Villarreal, Ruben Ruiz-Simon, Andrea R. Lopez-Pastor, Melina Vega de Ceniga, Leticia Esparza, Jose L. Martin-Ventura, Oscar Escribano, Almudena Gomez-Hernandez
Summary: MicroRNAs miR-15a-5p and miR-199a-3p are significantly decreased in aortic and carotid samples from atherosclerosis patients and mice. Overexpression of these miRNAs can reduce inflammation and progression of atherosclerosis. MiR-15a-5p may serve as a novel diagnostic biomarker for advanced atherosclerosis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Xiang-jun Chen, Sha Liu, Dong-mei Han, De-zhi Han, Wei-jing Sun, Xiao-chun Zhao, Jun-qing Liang, Li Yu
Summary: Melanoma is a major skin malignancy with unclear molecular drivers for progression and prognosis. FUT8-AS1 has been identified as a lncRNA related to melanoma prognosis, and its downregulation is associated with aggressive clinical factors. Functionally, FUT8-AS1 suppresses melanoma cell proliferation, migration, and invasion by regulating the NF90/miR-145-5p/NRAS/MAPK signaling axis, highlighting its potential as a therapeutic target for melanoma treatment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Jishui Zhang, Wenhao Lv, Yagang Liu, Weihua Fu, Baosheng Chen, Qiutong Ma, Xin Gao
Summary: This study demonstrated that LINC_00355 promotes GC progression by up-regulating PHF19 through sponging miR-15a-5p. Knockdown of LINC_00355 inhibited cell viability, migration, invasion, and induced apoptosis in GC cells, while overexpression had the opposite effects. PHF19 overexpression reversed the effect of LINC_00355 knockdown on GC cell properties. These findings provide important insights for potential clinical intervention in reversing the malignant phenotype of GC.
Review
Biochemistry & Molecular Biology
Dada Wen, Huamin Zhang, Yutong Zhou, Jie Wang
Summary: This article reviews the important role and regulatory mechanisms of miR-15a/16 in fibrotic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Jihoon Tak, Yun Seok Kim, Tae Hyun Kim, Gil-Chun Park, Shin Hwang, Sang Geon Kim
Summary: This study demonstrates that ER stress-induced overexpression of G alpha(12) in hepatocytes promotes ferroptosis through dysregulation of ALOX12 mediated by ROCK1, as well as decreased miR-15a. Inhibition of G alpha(12) overexpression and/or the ROCK1 axis may be a promising therapeutic strategy for acute liver injury.
Article
Cell Biology
Yang Cao, Jiacheng Wu, Ye Hu, Yangyang Chai, Jiaying Song, Jiaqi Duan, Song Zhang, Xiaoqing Xu
Summary: A recently identified lncRNA, lncRNA-BTX, is found to promote viral replication and immune escape by enhancing the interactions between specific RBPs. This discovery unveils a strategy developed by viruses to evade host innate immunity, and provides a potential target for antiviral therapeutics.
Article
Agriculture, Dairy & Animal Science
Jinhui Bai, Hongyan Xu, Jiachen Fang, Congcong Zhang, Jixuan Song, Xinxin Zhang, Beibei Hao, Baozhen Yin, Guangjun Xia
Summary: In this study, miR-15a was found to regulate the differentiation of Yanbian yellow cattle preadipocytes by targeting ABAT and affecting fat metabolism. These findings provide new targets for investigating intramuscular fat deposits in cattle and treating human obesity.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Wenxin Qi, Qian Liu, Wenjun Fu, Jiaming Shi, Minmin Shi, Songqi Duan, Zhe Li, Shaohua Song, Jiao Wang, Yihao Liu
Summary: This study found that BHLHE40 is highly expressed in pancreatic cancer tissues and is associated with poor prognosis of patients. Further experiments revealed that BHLHE40 may be involved in immunosuppression of pancreatic cancer. Silencing BHLHE40 can inhibit the proliferation, invasion, and apoptosis of pancreatic cancer cells, suggesting that BHLHE40 is a potential therapeutic target for pancreatic cancer.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Carlos Garcia-Padilla, Jorge N. Dominguez, Valeria Lodde, Rachel Munk, Kotb Abdelmohsen, Myriam Gorospe, Veronica Jimenez-Sabado, Antonino Ginel, Leif Hove-Madsen, Amelia E. Aranega, Diego Franco
Summary: Atrial fibrillation (AF) is the most common cardiac arrhythmia in humans. Recent research shows that the Pitx2 transcription factor plays a crucial role in regulating the expression of growth factors, microRNAs, and ion channels, which contribute to the onset of AF. Additionally, several lncRNAs have been discovered to be involved in AF development and are regulated by different signaling pathways.
Article
Multidisciplinary Sciences
Valeria Lodde, Matteo Floris, Rachel Munk, Jennifer L. Martindale, Davide Piredda, Catello Mario Panu Napodano, Francesco Cucca, Sergio Uzzau, Kotb Abdelmohsen, Myriam Gorospe, Ji Heon Noh, M. Laura Idda
Summary: NF90, an RNA-binding protein, plays a critical role in RNA metabolism, particularly in viral infection and immune response. Studies have shown that NF90 can influence the expression of key genes such as IRF3 and IRF9, thereby modulating the interferon response to SARS-CoV-2 infection.
SCIENTIFIC REPORTS
(2022)
Article
Geriatrics & Gerontology
Hailiang Mei, Jasper Boom, Salma el Abdellaoui, Kotb Abdelmohsen, Rachel Munk, Jennifer L. Martindale, Susan Kloet, Szymone M. Kielbasa, Thomas H. Sharp, Myriam Gorospe, Vered Raz
Summary: Aging-associated muscle wasting is regulated by multiple molecular processes, and aberrant mRNA processing regulation is one of the causes of muscle wasting. The poly(A)-binding protein nuclear 1 (PABPN1) plays a key role in regulating polyadenylation site (PAS) utilization. This study shows that a persistent reduction in PABPN1 levels leads to a significant loss of translation efficiency, which in turn affects protein homeostasis and contributes to aging-associated muscle atrophy.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Ruin Moaddel, Martina Rossi, Stephanie Rodriguez, Rachel Munk, Mohammed Khadeer, Kotb Abdelmohsen, Myriam Gorospe, Luigi Ferrucci
Summary: Senescent cells, which accumulate with aging, have been shown to contribute to age-associated diseases and organ dysfunction. In this study, ginger extract was found to selectively eliminate senescent cells without affecting normal cells. The major components of ginger extract, gingerenone A and 6-shogaol, showed promising senolytic properties and also suppressed the senescence-associated secretory phenotype (SASP). These findings suggest that gingerenone A may have therapeutic benefit in diseases characterized by senescent cell accumulation.
Article
Multidisciplinary Sciences
Carlos Anerillas, Allison B. Herman, Martina Rossi, Rachel Munk, Elin Lehrmann, Jennifer L. Martindale, Chang-Yi Cui, Kotb Abdelmohsen, Supriyo De, Myriam Gorospe
Summary: This study analyzed the response of human fibroblasts to different doses of the genotoxin etoposide and found that SRC is a crucial kinase in determining whether the cells undergo apoptosis or senescence. SRC is activated by low levels of DNA damage, leading to cell survival and senescence, but not by high levels, resulting in apoptosis. Inhibition of SRC prevents the accumulation of senescent cells in tissues exposed to DNA damage, suggesting that targeting SRC could improve health outcomes by promoting apoptosis over senescence.
Article
Biochemistry & Molecular Biology
Jen-Hao Yang, Ming-Wen Chang, Dimitrios Tsitsipatis, Xiaoling Yang, Jennifer L. Martindale, Rachel Munk, Aiwu Cheng, Elizabeth Izydore, Poonam R. Pandey, Yulan Piao, Krystyna Mazan-Mamczarz, Supriyo De, Kotb Abdelmohsen, Myriam Gorospe
Summary: In this study, the researchers discovered a noncoding RNA regulatory network that promotes myoblast fusion into multinucleated myotubes by modulating the degradation of miR-7.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Ophthalmology
Rita Serra, Vincenzo Rallo, Antonio Pinna, Maristella Steri, Maria Grazia Piras, Michele Marongiu, Florence Coscas, Myriam Gorospe, David Schlessinger, Edoardo Fiorillo, Francesco Cucca, Andrea Angius
Summary: This study investigated the prevalence and clinical and genetic features of age-related macular degeneration (AMD) in a population living in the Lanusei valley, Central Sardinia, Italy. The results showed that most subjects did not show any signs of AMD, and late AMD was rare. The study supports the hypothesis that the Sardinian population may have a protective background against AMD development.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michele Marongiu, Gonzalo Perez-Mejias, Valeria Orru, Maristella Steri, Carlo Sidore, Antonio Diaz-Quintana, Antonella Mulas, Fabio Busonero, Andrea Maschio, Klaudia Walter, Manuel Tardaguila, Parsa Akbari, Nicole Soranzo, Edoardo Fiorillo, Myriam Gorospe, David Schlessinger, Irene Diaz-Moreno, Francesco Cucca, Magdalena Zoledziewska
Summary: This study conducted a genome-wide association study (GWAS) on the genetic regulation of morphological traits of white blood cells. It identified seven significant signals, four of which were novel and one of them had a large effect size on eosinophil morphology. Computational analysis revealed a mutation in the PRG2 gene that could possibly explain the observed changes in eosinophil morphology.
HUMAN MOLECULAR GENETICS
(2023)
Article
Immunology
Maria Laura Idda, Maristella Pitzalis, Valeria Lodde, Annalisa Loizedda, Jessica Frau, Monia Lobina, Magdalena Zoledziewska, Francesca Virdis, Giuseppe Delogu, Maria Giuseppina Marini, Maura Mingoia, Marco Masala, Lorena Lorefice, Marzia Fronza, Daniele Carmagnini, Elisa Carta, Silvy Pilotto, Paolo Castiglia, Paola Chessa, Sergio Uzzau, Gabriele Farina, Paolo Solla, Maristella Steri, Marcella Devoto, Edoardo Fiorillo, Matteo Floris, Roberto Ignazio Zarbo, Eleonora Cocco, Francesco Cucca
Summary: Monitoring immune responses to SARS-CoV-2 vaccination and its clinical efficacy over time in Multiple Sclerosis (MS) patients treated with disease-modifying therapies (DMTs) helps to establish optimal strategies for COVID-19 protection without compromising disease control. A study found that humoral responses decreased in MS patients treated with certain therapies, but significantly increased one month after the booster. Although patients on specific treatments had a higher rate of COVID-19 infection, only mild symptoms were reported.
FRONTIERS IN IMMUNOLOGY
(2022)
Correction
Multidisciplinary Sciences
Carlos Anerillas, Allison B. Herman, Rachel Munk, Amanda Garrido, Kwan-Wood Gabriel Lam, Matthew J. Payea, Martina Rossi, Dimitrios Tsitsipatis, Jennifer L. Martindale, Yulan Piao, Krystyna Mazan-Mamczarz, Jinshui Fan, Chang-Yi Cui, Supriyo De, Kotb Abdelmohsen, Rafael de Cabo, Myriam Gorospe
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Valeria Lodde, Matteo Floris, Enrico Zoroddu, Ignazio Roberto Zarbo, Maria Laura Idda
Summary: Autoimmune diseases are caused by the immune system's loss of tolerance to the body's own cells and tissues. RNA-binding proteins play a critical role in regulating immunity by modulating gene expression post-transcriptionally. This review explores the connection between the genetics and molecular biology of RNA-binding proteins in multiple sclerosis, type 1 diabetes mellitus, systemic lupus erythematosus, and rheumatoid arthritis. Understanding the genetics and biology of autoimmune diseases can lead to the discovery of new therapies.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Multidisciplinary Sciences
Sunil K. Ahuja, Muthu Saravanan Manoharan, Grace C. Lee, Lyle R. McKinnon, Justin A. Meunier, Maristella Steri, Nathan Harper, Edoardo Fiorillo, Alisha M. Smith, Marcos I. Restrepo, Anne P. Branum, Matthew J. Bottomley, Valeria Orru, Fabio Jimenez, Andrew Carrillo, Lavanya Pandranki, Caitlyn A. Winter, Lauryn A. Winter, Alvaro A. Gaitan, Alvaro G. Moreira, Elizabeth A. Walter, Guido Silvestri, Christopher L. King, Yong-Tang Zheng, Hong-Yi Zheng, Joshua Kimani, T. Blake Ball, Francis A. Plummer, Keith R. Fowke, Paul N. Harden, Kathryn J. Wood, Martin T. Ferris, Jennifer M. Lund, Mark T. Heise, Nigel Garrett, Kristen R. Canady, Salim S. Abdool Karim, Susan J. Little, Sara Gianella, DaveyM. Smith, Scott Letendre, Douglas D. Richman, Francesco Cucca, Hanh Trinh, Sandra Sanchez-Reilly, Joan M. Hecht, Jose A. Cadena Zuluaga, Antonio Anzueto, JacquelineA. Pugh, Brian K. Agan, Robert Root-Bernstein, Robert A. Clark, Jason F. Okulicz, Weijing He
Summary: Some individuals have better health throughout life, possibly due to optimal immune resilience. The preservation of optimal immune resilience is associated with a lower risk of various diseases and improved health outcomes.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Martina Rossi, Carlos Anerillas, Maria Laura Idda, Rachel Munk, Chang Hoon Shin, Stefano Donega, Dimitrios Tsitsipatis, Allison B. Herman, Jennifer L. Martindale, Xiaoling Yang, Yulan Piao, Krystyna Mazan-Mamczarz, Jinshui Fan, Luigi Ferrucci, Peter F. Johnson, Supriyo De, Kotb Abdelmohsen, Myriam Gorospe, Berenice A. Benayoun
Summary: Senescent cells release the senescence-associated secretory phenotype (SASP), which is a collection of cytokines, proteases, and growth factors associated with chronic inflammation and age-related diseases. This study explored the expression and role of the immunomodulatory cytokine BAFF in different senescence models. BAFF production was found to be elevated in various senescence paradigms, and the transcription factor IRF1 was identified as necessary for TNFSF13B mRNA transcription. Inhibition of BAFF production reduced the senescent phenotype and secretion of IL6 in fibroblasts and monocyte-like cells, with cell type-specific effects on NF-kappa B activation and TP53 function.
Article
Cell Biology
Noah Wechter, Martina Rossi, Carlos Anerillas, Dimitrios Tsitsipatis, Yulan Piao, Jinshui Fan, Jennifer L. Martindale, Supriyo De, Krystyna Mazan-Mamczarz, Myriam Gorospe
Summary: Senescence is a state of enduring growth arrest triggered by sublethal cell damage, and the accumulation of senescent cells in older individuals has been linked to age-related diseases. However, identifying robust markers of senescence has been challenging due to the heterogeneous and dynamic nature of the senescent phenotype. In this study, single-cell RNA-sequencing analysis was used to comprehensively analyze the senescent transcriptome of human diploid fibroblasts, revealing distinct cell subpopulations and dynamic changes in gene expression during senescence. This research provides a deeper understanding of senescent cell phenotypes and may lead to the development of more effective interventions.