Article
Microbiology
Giuseppe D. Tocchini-Valentini, Glauco P. Tocchini-Valentini
Summary: A homodimeric tRNA endonuclease from the euryarchaeota Ferroplasma acidarmanus has been characterized, showing the ability to cleave BHB substrates at low temperatures and create proteins with new functionalities through domain recombination.
Article
Multidisciplinary Sciences
Jennifer E. Hurtig, Michelle A. Steiger, Vinay K. Nagarajan, Tao Li, Ti-Chun Chao, Kuang-Lei Tsai, Ambro van Hoof
Summary: Recent studies show that yeast TSEN cleaves a specific subset of mRNAs encoding mitochondrial proteins, with cleavage sites determined by sequence. The development of the comPARE bioinformatic approach enables the identification of these mRNA target sites more effectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Xueling Lu, Fengtao Huang, Rui Cheng, Bin Zhu
Summary: In this study, the first virus-derived modification-dependent restriction endonuclease (HHPV4I) from the archaeal virus HHPV4 was reported. HHPV4I recognizes Gm6ATC sites and specifically binds to Gm6ATC site-containing DNA. Unlike the well-known m6A-specific restriction enzyme DpnI, HHPV4I cleaves DNA upstream and downstream of the fully methylated Gm6ATC site and preferentially cleaves DNA between VR bases. Mutations in the HHPV4I wH domain alter sequence specificity without affecting m6A-dependent endonuclease activity, providing a target for engineering m6A-dependent endonucleases with different specificities.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Ling Yuan, Yaoyao Han, Jiazheng Zhao, Yixiao Zhang, Yadong Sun
Summary: The removal of introns from transfer RNA precursors is vital for tRNA maturation and functionality. In this study, the researchers present the cryo-EM structure of an active human tRNA splicing endonuclease complex bound to an intron-containing pre-tRNA. They uncover the molecular mechanism for eukaryotic pre-tRNA recognition and cleavage, as well as the evolutionary relationship between archaeal and eukaryotic TSENs.
NATURE COMMUNICATIONS
(2023)
Article
Genetics & Heredity
Jennifer E. Hurtig, Ambro van Hoof
Summary: Through yeast genetics, it is discovered that TSEN not only participates in tRNA splicing but also has other functions, including degradation of a subset of mRNA encoding mitochondrial proteins and an unknown essential function. Overexpression of SEN54 can suppress sen2 mutants, indicating its general suppressive effect on sen2, regardless of tRNA-dependent or tRNA-independent functions. Mutations in the intron-debranching enzyme Dbr1 can also serve as tRNA splicing-independent suppressors. These findings provide insights into the essential function(s) of TSEN.
Article
Genetics & Heredity
Ambuj Srivastava, Dhanusha Yesudhas, Shandar Ahmad, M. Michael Gromiha
Summary: This study demonstrates that the disorder-to-order transition regions play crucial roles in stabilizing the Trm5-tRNA complex. The DOT1 and DOT2 residues are important for stabilizing the complex, while DOT3 coordinates interactions near the active site. Mutational studies show that the wild-type complex is more stable than the G37A tRNA mutant complex, with Arg145 in DOT3 playing a key role in stabilizing the ligand and G37 nucleotide. Overall, targeting the DOT regions could potentially improve inhibition of Trm5.
Article
Biology
Yuya Taguchi, Takahiro Yamasaki, Marie Ishikawa, Yuki Kawasaki, Ryuji Yukimura, Maki Mitani, Kunio Hirata, Daisuke Kohda
Summary: The study elucidates the structural basis for the exclusion of proline residues from N-glycosylation sequons by oligosaccharyltransferase, highlighting the essential role of the TIXE motif and its adjacent regions in this process. This provides valuable insight into the sequon specificity of N-glycosylation, a major posttranslational modification in eukaryotes.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pierre Aldag, Fabian Welzel, Leonhard Jakob, Andreas Schmidbauer, Marius Rutkauskas, Fergus Fettes, Dina Grohmann, Ralf Seidel
Summary: CRISPR-Cas9 is a versatile ribonucleoprotein complex used in various biological techniques, but its stable binding to DNA after cleavage poses a challenge in genome editing applications. Research shows that different Cas9 variants exhibit different release mechanisms after DNA cleavage, with post-cleavage state demonstrating higher stability. This study provides insights into the binding stability of Cas9 and its variants, shedding light on their potential applications in genome editing.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Oncology
Satoru Shinriki, Hirotaka Matsui
Summary: Mutations in DDX41 play a role in myeloid leukemogenesis by affecting various cellular processes including RNA splicing, nucleic acid sensing, R-loop resolution, and snoRNA processing. The distinctive disease phenotype associated with DDX41 mutations suggests its involvement in the pathogenesis of myeloid malignancies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael C. Yoon, Vivian Hook, Anthony J. O'Donoghue
Summary: Cathepsin B exhibits DPCP and endopeptidase activities under both acidic and neutral pH conditions. Cleavage profiling reveals distinct pH-dependent amino acid preferences for DPCP and endopeptidase activities. The improved activity of cathepsin B at neutral pH and acidic pH is achieved by designing new substrates.
Article
Biochemistry & Molecular Biology
Fumitaka Kudo, Takuji Chikuma, Mizuki Nambu, Taichi Chisuga, Shimpei Sumimoto, Arihiro Iwasaki, Kiyotake Suenaga, Akimasa Miyanaga, Tadashi Eguchi
Summary: In this study, we analyzed the genome sequence of Moorena bouillonii, a marine cyanobacterium that produces lyngbyapeptin B, and identified a gene cluster responsible for its biosynthesis. We characterized two unique enzymatic activities: O-methylation by LynB2 and oxidative decarboxylation by LynB7. These findings provide insights into the biosynthetic mechanisms of lyngbyapeptin B.
ACS CHEMICAL BIOLOGY
(2023)
Article
Reproductive Biology
Yohei Hayashi, Masaru Mori, Kaori Igarashi, Keiko Tanaka, Asuka Takehara, Yumi Ito-Matsuoka, Akio Kanai, Nobuo Yaegashi, Tomoyoshi Soga, Yasuhisa Matsui
BIOLOGY OF REPRODUCTION
(2020)
Article
Genetics & Heredity
Trieu-Duc Vu, Yuki Lwasaki, Shuji Shigenobu, Akiko Maruko, Kenshiro Oshima, Erica Lioka, Chao-Li Huang, Takashi Abe, Satoshi Tamaki, Yi-Wen Lin, Chih-Kuan Chen, Mei-Yeh Lu, Masaru Hojo, Hao-Ven Wang, Shun-Fen Tzeng, Hao-Jen Huang, Akio Kanai, Takashi Gojobori, Tzen-Yuh Chiang, H. Sunny Sun, Wen-Hsiung Li, Norihiro Okada
Article
Biochemical Research Methods
Vyacheslav Tretyachenko, Vaclav Voracek, Radko Soucek, Kosuke Fujishima, Klara Hlouchova
Summary: CoLiDe is a computational tool that offers precise control over protein sequence composition, length, and diversity for designing combinatorial libraries. It uses an evolutionary approach to provide solutions for degenerate DNA templates and can prepare purified protein library samples with up to 10^11 - 10^12 unique sequences. CoLiDe presents a composition-centric approach to protein design for different functional phenomena.
Article
Biotechnology & Applied Microbiology
Sabrina Galinanes Reyes, Yutetsu Kuruma, Mai Fujimi, Masako Yamazaki, Sumie Eto, Shota Nishikawa, Satoshi Tamaki, Asaki Kobayashi, Ryo Mizuuchi, Lynn Rothschild, Mark Ditzler, Kosuke Fujishima
Summary: The refined PURE-based display method was developed for the preparation of mRNA and cDNA-peptide conjugates, showing efficient enrichment of FLAG epitope motifs, especially in mRNA display. The study demonstrated that gel purification steps improve conjugate formation efficiency and enhance the enrichment rate of specific epitope motifs. Overall, the research suggests the potential of the PURE system for exploring diverse polypeptide sequences and functional space in future studies.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Ryota Yamagami, Jacob P. Sieg, Philip C. Bevilacqua
Summary: RNA regulates various cellular events and relies on magnesium ions to fold into complex structures. Recent studies show that weakly chelated magnesium ions can enhance RNA function, indicating the importance of considering chelated magnesium in future RNA and protein research.
Article
Biochemistry & Molecular Biology
Yu Nishida, Shiho Ohmori, Risa Kakizono, Kunpei Kawai, Miyu Namba, Kazuki Okada, Ryota Yamagami, Akira Hirata, Hiroyuki Hori
Summary: The Saccharomyces cerevisiae Trm11-Trm112 complex methylates the 2-amino group of guanosine at position 10 in tRNA, and this study investigates the elements required for methylation. The results show that precursor tRNA, tRNA with a special structure, and tRNA lacking the CCA terminus or regular-size variable region are not substrates for Trm11-Trm112. Additionally, the G10-C25 base pair is necessary for methylation, and Trm11-Trm112 also recognizes the anticodon-loop. The study provides insights into the m(2)G10 modification pattern of S. cerevisiae class I tRNAs and identifies Trm11-Trm112 binding sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Masahiro C. Miura, Shohei Nagata, Satoshi Tamaki, Masaru Tomita, Akio Kanai
Summary: This study developed a bioinformatic pipeline to systematically collect and analyze Group II introns (G2Is) in prokaryotes. The results showed the presence of G2Is in both bacteria and archaea, with their numbers defined almost at the phylum level. Furthermore, a comprehensive sequence analysis led to the identification of three new categories of intron-encoded proteins (IEPs), and it was observed that about 30% of IEPs are non-canonical. The study also analyzed the relationship between genomic signatures and the mechanism of G2I increases.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ryota Yamagami, Jacob P. Sieg, Sarah M. Assmann, Philip C. Bevilacqua
Summary: RNA structure is crucial for cellular events, and the study of tRNA structure is challenging. The tRNA structure-seq workflow accurately determines the in vivo secondary structure of tRNA and provides insights into tRNA abundance and natural modifications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Megumi Tsurumaki, Motofumi Saito, Masaru Tomita, Akio Kanai
Summary: The candidate phyla radiation (CPR) bacteria, consisting mainly of uncultured lineages, have smaller cells and genomes compared to non-CPR bacteria. They lack certain ribosomal proteins and exhibit unique protein lengths and sequences. The ribosomes of CPR bacteria appear to be smaller with simplified surface structures, which may contribute to their distinct characteristics.
Article
Microbiology
Teppei Morita, Nadim Majdalani, Masahiro C. Miura, Rerina Inose, Taku Oshima, Masaru Tomita, Akio Kanai, Susan Gottesman
Summary: The regulatory function of many bacterial small RNAs (sRNAs) is dependent on the RNA chaperone Hfq binding to the 3' portion of the sRNA intrinsic terminator. This study identified several genes, including an sRNA gene (cyaR) and three protein-coding genes (cspD, ygjH, and rof), that attenuate the termination of SgrS in Escherichia coli. Overexpression of the protein attenuators CspD and Rof resulted in increased readthrough at terminators of SgrS and other sRNAs, reducing their regulatory effects on target mRNAs. Further analysis revealed the unexpected role of Rho in sRNA termination and the stabilizing effect of CspD on both terminated and readthrough transcripts. This study provides evidence of the modulation of intrinsic termination as an additional layer of regulation in sRNA signaling.
Article
Biochemistry & Molecular Biology
Ryota Yamagami, Hiroyuki Hori
Summary: Transfer RNAs undergo diverse posttranscriptional modifications to regulate cellular events. Recent RNA-seq techniques provide insights into tRNA features. This study adapts a tRNA-sequencing technique to analyze tRNA modifications and gain mechanistic insights into enzyme-substrate recognition.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yuzuru Sugio, Ryota Yamagami, Naoki Shigi, Hiroyuki Hori
Summary: The modified nucleoside s(4)U is found in tRNA from bacteria and archaea and has been used as a biotechnological tool for RNA analysis. In this study, a sensitive and selective system for the detection of s(4)U was developed using biotin-coupled MTSEA biotin-XX. The system can analyze multiple samples in a short time span and can also be used to visualize newly transcribed tRNAs.
Article
Biochemistry & Molecular Biology
Motofumi Saito, Rerina Inose, Asako Sato, Masaru Tomita, Haruo Suzuki, Akio Kanai
Summary: The Clp1 family proteins play an essential role in RNA processing in eukaryotes, and their diversification is mainly due to gene duplication and alternative splicing. Our study reveals that the Clp1 and Nol9/Grc3 groups were already formed in the eukaryotic ancestor, and the protist phylum Euglenozoa has a particularly high number of Clp1 family proteins. This research provides valuable insights into the evolutionary history of Clp1 family proteins in different species.
JOURNAL OF MOLECULAR EVOLUTION
(2023)
Review
Geography, Physical
Naota Takeyama, Yuka Takahashi, Shohei Nagata, Yusuke Sawaki, Tomohiko Sato, Shigenori Maruyama, Akio Kanai
JOURNAL OF GEOGRAPHY-CHIGAKU ZASSHI
(2020)
Review
Geography, Physical
Megumi Tsurumaki, Motofumi Saito, Shigenori Maruyama, Akio Kanai
JOURNAL OF GEOGRAPHY-CHIGAKU ZASSHI
(2020)