Article
Biochemical Research Methods
Niina Smolander, Timothy R. Julian, Manu Tamminen
Summary: Prider is an R package for designing primers and probes for complex and large sequence sets. It efficiently solves the problem of covering maximal sequence diversity with minimal oligonucleotide primers or probes. The resulting primers can be easily accessed and visualized using Prider's plotting function. This tool has great potential in fields such as clinical microbiology, epidemic virus surveillance, and antimicrobial resistance surveillance.
BMC BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Marina Mariconti, Mathieu Morel, Damien Baigl, Sergii Rudiuk
Summary: Hybrid DNA-protein nanogels were successfully prepared with controllable size and density using a simple method involving highly biotinylated DNA as a backbone and streptavidin-biotin binding for cross-linking. The resulting nanogels could be adjusted in size and density by changing the amount of cross-linking streptavidin and the rate of DNA biotinylation. Incorporation of enzymes like AP, HRP, and beta Gal into the nanogels showed retained functional activity, with HRP activity slightly improved, indicating that the physical density of the functionalized DNA nanogels plays a crucial role in enhancing enzymatic activity.
Article
Chemistry, Multidisciplinary
Zhuoyuan Zhang, Jianwei Lin, Zheng Liu, Gaofei Tian, Xiao-Meng Li, Yihang Jing, Xin Li, Xiang David Li
Summary: This article reviews recent advances in using photo-crosslinking strategies to investigate the epigenetic interactome, focused on the protein-protein interactions mediated by epigenetic marks in histone tails. The future directions include developing photo-crosslinking-based tools and methods for studying binding events in nucleosomal/chromatinic contexts, and for capturing the epigenetic interactome in live cells or even organisms.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Applied
Dominik Ruhr, Michael John, Annette Reiche
Summary: Experimental study showed that approximately 50% of BDDE molecules effectively cross-link, and there is a direct correlation between the degree of cross-linking and the decomposition temperature of the cellulose membranes.
CARBOHYDRATE POLYMERS
(2021)
Article
Nanoscience & Nanotechnology
Wenpin Wang, Min Xu, Shubo Wang, Xiaofeng Xie, Yafei Lv, Vijay Ramani
Summary: A series of cross-linked fluorinated poly(aryl ether oxadiazole) membranes derivatized with imidazolium groups were prepared using poly(N-vinylimidazole) (PVI) as the bifunctional cross-linking agent. The resulting membrane showed high ion exchange capacity, low vanadium permeability, and good dimensional stability. It also exhibited high chemical stability in acidic and oxidizing environments, and demonstrated superior electrochemical performance in vanadium redox flow battery testing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Organic
Heli Fan, Xiaohua Peng
Summary: Two small libraries of 2-OMe or 2-NO2-benzene analogues containing various leaving groups were synthesized and characterized, with compounds containing a 2-methoxy substituent showing faster cross-linking reaction rates and higher efficiency. The benzylic leaving groups greatly affected DNA cross-linking efficiency, with compounds containing OCH3, OCH2Ph, or Ph3P+ as leaving groups showing higher ICL yields. The heat stability study indicated that dGs were the preferred alkylation sites in DNA for the benzyl cations produced from certain compounds.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Energy & Fuels
Yandong Xie, Xiaotong Wang, Lijie Hou, Xiaoqiong Wang, Yan Zhang, Cuimei Zhu, Zhongai Hu, Miao He
Summary: The research introduces a novel pillared graphene network with increased active sites and capacitance for energy storage. By covalently grafting DAAQ onto graphene sheets, the DAAQ-PGN electrode exhibits a higher specific capacitance, offering new ideas for the development of supercapacitor materials.
JOURNAL OF ENERGY STORAGE
(2021)
Article
Chemistry, Applied
Esra Demirkiran, Bulent Basyigit, Gulbahar Altun, Melike Yucetepe, Hidayet Saglam, Mehmet Karaaslan
Summary: The current study explores the potential benefits of microbial transglutaminase (MTG) modification on the techno-functional and hydrogel characteristics of sour cherry seed protein (SCSP). The results indicate that the cross-linking process with MTG improves the techno-functional features and the properties of hydrogels. The coexistence of MTG-SCSP and pectin shows a synergistic impact on the functional properties of gels.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Analytical
Qiong Hu, Jianwen Wan, Haocheng Wang, Xiaojing Cao, Shiqi Li, Yiyi Liang, Yilin Luo, Wei Wang, Li Niu
Summary: A boronate-affinity cross-linking method is demonstrated for the highly sensitive and selective detection of glycoconjugates. The method utilizes a methylene blue-tagged nucleic acid aptamer to selectively capture target glycoconjugates, followed by labeling a large number of ferrocene tags through boronate-affinity cross-linking. The dual-signal output from the methylene blue and ferrocene tags enables ratiometric detection. This method is simple, cost-effective, and possesses high robustness and reproducibility.
ANALYTICAL CHEMISTRY
(2022)
Article
Agricultural Engineering
Ebru Sarioglu, Emine A. Turhan, Selcan Karaz, Basak Bengu, Aziz Bicer, Tugay Yarici, Can Erkey, Erkan Senses
Summary: In this study, a two-step wood surface modification strategy based on microwave heating and UV crosslinking was demonstrated. The fibers were reacted with methacrylic anhydride to create methacrylated groups, facilitating covalent bonding between the fibers. The chemical cross-linking greatly improved the bonding between fibers even in wet conditions, resulting in enhanced mechanical properties and dimensional stability of the wood composites.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biology
Aaron J. Robinson, Geoffrey L. House, Demosthenes P. Morales, Julia M. Kelliher, La Verne Gallegos-Graves, Erick S. LeBrun, Karen W. Davenport, Fabio Palmieri, Andrea Lohberger, Danae Bregnard, Aislinn Estoppey, Matteo Buffi, Christophe Paul, Thomas Junier, Vincent Herve, Guillaume Cailleau, Simone Lupini, Hang N. Nguyen, Amy O. Zheng, Luciana Jandelli Gimenes, Saskia Bindschedller, Debora F. Rodrigues, James H. Werner, Jamey D. Young, Pilar Junier, Patrick S. G Chain
Summary: Robinson et al. explored bacterial associations in over 700 phylogenetically diverse fungal isolates, using 16S rRNA sequencing of fungal collections from across the globe and bioinformatic screening of publicly available fungal genome sequencing projects. This study reveals that bacterial associations with fungal hosts are widespread, representing a significant increase in explored fungal-bacterial relationships.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Yuqian Guo, Yaofeng Zhou, Jinmei Fu, Hao Fang, Yu Li, Xiaolin Huang, Yonghua Xiong
Summary: A novel multiplex fluorescent competitive immunosensor was developed using self-luminous bacteria as bifunctional probes for simultaneous detection and quantification of multiple pathogens. By integrating a pre-incubation step, the method achieved ultrasensitive detection of pathogens in real milk with a detection time of less than 10 hours.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Fang Fu, Wei Lu, Yue Zheng, Kai Chen, Chen Sun, Lina Cong, Yulong Liu, Haiming Xie, Liqun Sun
Summary: A bifunctional regular-random dual cross-linking network solid polymer electrolyte (RRa-SPE) is proposed for dendrite-free all-solid-state lithium metal batteries, effectively regulating lithium deposition and facilitating the generation of a highly homogeneous and robust solid-electrolyte interphase (SEI) layer. The RRa-SPE exhibits good ionic conductivity, high lithium ion transference number, and enhanced mechanical strength, leading to low voltage polarization and excellent cycle performance in LiFePO4/RRa-SPE/Li cells.
JOURNAL OF POWER SOURCES
(2021)
Article
Biochemistry & Molecular Biology
Zhi Huang, Dandan Wang, Jinrui Zhou, Hong He, Cong Wei
Summary: Modified FISH technique can improve the weak or absent fluorescence signals of microbes, enabling a more precise identification and understanding of microbes in complex samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Wei-Hua Han, Qing-Yu Wang, Yuan-Yi Kang, Li-Rui Shi, Yu Long, Xin Zhou, Chun-Cheng Hao
Summary: This review comprehensively discusses the cross-linking electrospinning system for the first time, including cross-linking strategies, cross-linking mechanisms, and applications of cross-linked electrospun nanofibers. The significance of cross-linking electrospinning and the challenges in constructing this system are also highlighted.