Article
Plant Sciences
Rodrigo Porto Schwedersky, Marina de Lyra Soriano Saleme, Ingrid Andrade Rocha, Patricia da Fonseca Montessoro, Adriana Silva Hemerly, Nubia Barbosa Eloy, Paulo Cavalcanti Gomes Ferreira
Summary: The study reveals the crucial role of APC11 in early plant development, mediating overall plant growth by regulating APC/C activity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Su-Lin Zeng, Suraj S. Patel, Mo-Qi Lv, Daphne Zhu, Wen H. Shen, Li Liu
Summary: In this study, the novel oncogene STYK1/NOK's role in cell division was investigated. It was found that STYK1/NOK interacts directly with CDH1 and can affect cell division by targeting key components of the APC/C pathway. These findings provide mechanistic clues for the understanding of STYK1/NOK's roles in mitosis and cytokinesis.
Article
Biochemistry & Molecular Biology
Shan Jiang, Jinwei Wei, Na Li, Zhibiao Wang, Yilan Zhang, Ran Xu, Lixun Zhou, Xiahe Huang, Li Wang, Siyi Guo, Yingchun Wang, Chun-Peng Song, Wei Qian, Yunhai Li
Summary: This study identifies a regulatory cascade involving UBP14/DA3-CDKB1;1-CDKG2/SUD6 that plays a crucial role in controlling endoreduplication and cell growth in Arabidopsis.
Review
Biochemistry & Molecular Biology
Sanghati Roy Chowdhury, Tirthankar Koley, Mandeep Singh, Abdul Samath Ethayathulla, Punit Kaur
Summary: The complex molecular interactions leading to the oncogenic pathway are a result of cell cycle modification controlled by cell cycle regulatory proteins. Tumor suppressor and cell cycle regulatory proteins work together to maintain a healthy cellular environment. Heat shock proteins/chaperones, including Hsp90, play an important role in stabilizing tumor suppressors and cell cycle regulator proteins. Recent studies have shown that Hsp90 stabilizes mutant p53 and Fzr, which are crucial in regulating the cell cycle and preventing oncogenic processes. Dysfunction of this cellular process can lead to cancer development. Current drug trials targeting Hsp90 are discussed.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2023)
Article
Multidisciplinary Sciences
Yuji Nomoto, Hirotomo Takatsuka, Kesuke Yamada, Toshiya Suzuki, Takamasa Suzuki, Ying Huang, David Latrasse, Jing An, Magdolna Gombos, Christian Breuer, Takashi Ishida, Kenichiro Maeo, Miyu Imamura, Takafumi Yamashino, Keiko Sugimoto, Zoltan Magyar, Laszlo Bogre, Cecile Raynaud, Moussa Benhamed, Masaki Ito
Summary: The transcription factor SCL28 plays a critical role in regulating cell size and number during organ development by interacting with the transcription factor AtSMOS1 and promoting the transcription of SIAMESE-RELATED genes. This hierarchical transcriptional network allows for the precise control of cell size and number, leading to robust organ growth.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Shengqiang Di, Peng Zhang, Jiucheng Zhang, Genzhong Liu, Ge Wang, Qinghua Shi, Fangfang Ma, Zhilong Bao
Summary: The study reveals that SlUVI4 is involved in auxin-mediated hypocotyl elongation in tomato under heat stimulus, and plays an important role in inhibiting cell expansion.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Valentina Piano, Amal Alex, Patricia Stege, Stefano Maffini, Gerardo A. Stoppiello, Pim J. Huis in't Veld, Ingrid R. Vetter, Andrea Musacchio
Summary: The open and closed topologies of HORMA-domain proteins are associated with inactive and active cellular pathways, respectively. CDC20, an impervious substrate, requires simultaneous docking on several sites of the catalytic complex in order to accelerate its binding to MAD2, promoting MCC assembly. This may define a paradigm for other HORMA-controlled systems.
Article
Cell & Tissue Engineering
Trishita Basak, Rupasri Ain
Summary: This research reveals a multi-layered regulation of trophoblast self-renewal and differentiation by the Hippo components. YAP translocates to the nucleus in trophoblast stem cells and limits their proliferation by interacting with the stemness factor CDX2. Trophoblast giant cells differentiation is associated with cytoplasmic retention of YAP and decreased level of core Hippo component LATS1.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Xiangdong Hu, Xuejiao Jin, Xiuling Cao, Beidong Liu
Summary: The anaphase-promoting complex/cyclosome (APC/C) plays a significant role in regulating the cell cycle process and has potential implications in lifespan regulation and age-associated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Alex Willems, Lieven De Veylder
Summary: The review focuses on the plant APC/C complex, discussing its importance in plant development and its association with different developmental processes. The interactions between plant-specific APC/C activator subunits and other subunits are also described. Finally, a comprehensive list of confirmed and suspected plant APC/C target proteins is provided, offering opportunities to manipulate APC/C activity for increased crop yield and improved resilience to climate change.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Plant Sciences
Marina de Lyra Soriano Saleme, Ingrid Rocha Andrade, Nubia Barbosa Eloy
Summary: Most eukaryotic species propagate through sexual reproduction involving male and female gametes. In flowering plants, this process begins with DNA replication and chromosome segregation, followed by mitotic divisions to form male and female gametophytes. Precise regulation of gametogenesis progression is essential for successful meiotic cell division and sexual differentiation in plant species.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Xiaozhao Xu, Xuekun Wang, Kaisheng Zhang, Qin Yu, Xinqiang Jiang, Chenxia Cheng
Summary: In this study, 19 APC/C genes were identified in rose and their characteristics and expression patterns were investigated. The findings suggest that APC/C genes may play multiple roles in rose growth and development. Additionally, limitations in hormonal and abiotic stress responses were observed in the RhAPC/C genes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Pan Gong, Michiel Bontinck, Kirin Demuynck, Jolien De Block, Kris Gevaert, Dominique Eeckhout, Geert Persiau, Stijn Aesaert, Griet Coussens, Mieke Van Lijsebettens, Laurens Pauwels, Geert De Jaeger, Dirk Inze, Hilde Nelissen
Summary: SAMBA has been identified as a plant-specific regulator of the anaphase-promoting complex/cyclosome (APC/C) in Arabidopsis and maize. Two samba genome edited mutants in maize showed growth defects, with samba-1 representing a knockout allele and samba-3 producing a truncated protein that still interacted with APC/C but with altered activity. This suggests a dosage-dependent role for SAMBA in controlling developmental processes.
Article
Cell Biology
Li Chen, Ying-Chun Ouyang, Lin-Jian Gu, Jia-Ni Guo, Zhi-Ming Han, Zhen-Bo Wang, Yi Hou, Heide Schatten, Qing-Yuan Sun
Summary: This study demonstrates the critical role of Septin 9 in mouse oocyte meiotic cell cycle progression. Septin 9 regulates the kinetochore-microtubule connection and SAC protein localization on kinetochores, affecting the MI-AI transition as well as APC/C-CDC20 activity and CCNB1 degradation.
CELL PROLIFERATION
(2023)
Article
Multidisciplinary Sciences
Eyashin Ali, Manash Jyoti Kalita, Simanta Kalita, Jayasree Talukdar, Ankur Jyoti Deka, Jasmin Sultana, Bikash Narayan Choudhury, Munindra Narayan Baruah, Sahana Bhattacharjee, Subhash Medhi
Summary: Esophageal squamous cell carcinoma (ESCC) is a prevalent type of esophageal cancer worldwide with a poor prognosis. Dysregulation of anaphase promoting complex/cyclosome (APC/C) activation due to altered expression of APC molecules may contribute to the development of ESCC. The study analyzed the expression of APC/C complex subunits and found significant upregulation of APC7 and APC3, which suggested their role in uncontrolled cell proliferation in ESCC. Additionally, lifestyle factors and their association with APC expression were also studied.
Article
Chemistry, Multidisciplinary
Yusaku Hontani, Mikhail Baloban, Francisco Velazquez Escobar, Swetta A. Jansen, Daria M. Shcherbakova, Jorn Weissenborn, Miroslav Kloz, Maria Andrea Mroginski, Vladislav V. Verkhusha, John T. M. Kennis
Summary: By using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics calculations, the authors analyzed the real-time binding process of biliverdin to miRFP proteins. This study revealed the steps of isomerization, localization, and protonation of biliverdin within minutes, providing insights for the rational design of near-infrared fluorescent proteins.
COMMUNICATIONS CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Bettina Szerencses, Attila Gacser, Gabriella Endre, Ildiko Domonkos, Hilda Tiricz, Csaba Vagvolgyi, Janos Szolomajer, Dian H. O. Howan, Gabor K. Toth, Ilona Pfeiffer, Eva Kondorosi
Summary: This study demonstrates the anticandidal activity of NCR335 and NCR169 peptide derivatives against five Candida species, showing the ability to inhibit biofilm formation and yeast-hypha transformation. Combined treatment with fluconazole revealed synergistic interactions and reduced minimal inhibitory concentrations of C. auris. Shortening NCR peptides can enhance and broaden their anticandidal activity and therapeutic potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Olena S. Oliinyk, Mikhail Baloban, Charles L. Clark, Erin Carey, Sergei Pletnev, Axel Nimmerjahn, Vladislav V. Verkhusha
Summary: miRFP670nano3 is a near-infrared fluorescent protein that can be used to develop fluorescent nanobodies, whose stability and fluorescence depend on antigen binding, with broad implications for detecting and manipulating cellular targets, and it has important research significance.
Article
Biotechnology & Applied Microbiology
Adrian Wallner, Nicolas Busset, Joy Lachat, Ludivine Guigard, Eoghan King, Isabelle Rimbault, Peter Mergaert, Gilles Bena, Lionel Moulin
Summary: This study compared the genetic strategies used by B. vietnamiensis and P. kururiensis to colonize different varieties of rice, revealing that B. vietnamiensis has a higher number of genes involved in rice root colonization compared to P. kururiensis, and rice shows a stronger adverse reaction toward B. vietnamiensis. The bacterial fitness depends on a higher number of genes when colonizing different rice varieties.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Cell Biology
Maksim M. M. Karasev, Mikhail Baloban, Vladislav V. V. Verkhusha, Daria M. M. Shcherbakova
Summary: Researchers developed a non-opsin optogenetic tool for light-controlled nuclear export of a protein in neurons. They found that commonly used nuclear localization signals (NLSs) are suboptimal in neurons and identified uncommon NLSs that mediate fast nuclear import. By adjusting the NLSs, they optimized the tool for lower background and higher contrast in neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Dmitrii Y. Travin, Dmitry Sutormin, Peter Mergaert, Konstantin Severinov
Summary: In this study, we present the whole-genome sequences of two rhizobia strains that produce narrow-spectrum antirhizobial azol(in)e-modified peptides.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Correction
Biochemical Research Methods
Olena S. S. Oliinyk, Chenshuo Ma, Sergei Pletnev, Mikhail Baloban, Carlos Taboada, Huaxin Sheng, Junjie Yao, Vladislav V. V. Verkhusha
Article
Biochemical Research Methods
Olena S. Oliinyk, Chenshuo Ma, Sergei Pletnev, Mikhail Baloban, Carlos Taboada, Huaxin Sheng, Junjie Yao, Vladislav V. Verkhusha
Summary: Using rational design, we developed a 17 kDa cyanobacteriochrome-based near-infrared (NIR-I) fluorescent protein, miRFP718nano, which efficiently binds endogenous biliverdin chromophore and emits bright fluorescence in mammalian cells and tissues. With a maximal emission at 718 nm and an emission tail in the short-wave infrared (SWIR) region, miRFP718nano enables deep-penetrating off-peak fluorescence imaging in vivo. We demonstrated the superiority of miRFP718nano-enabled SWIR imaging in various applications, including imaging of microbes in the mouse digestive tract, mammalian cells injected into the mouse mammary gland, and NF-kB activity in a mouse model of liver inflammation.
Article
Biochemistry & Molecular Biology
Olena S. Oliinyk, Sergei Pletnev, Mikhail Baloban, Vladislav V. Verkhusha
Summary: Researchers converted the GAF domain of NpR3784 cyanobacteriochrome into near-infrared fluorescent proteins. By engineering, these proteins can bind biliverdin abundant in mammalian cells, making it the smallest scaffold for it. They determined the crystal structure of miRFP670nano3, the brightest blue-shifted protein, and explained its spectral properties. Using this structure, they designed a red-shifted NIR FP called miRFP704nano, which shows promising characteristics for multiplexed imaging in mammalian cells.
Article
Microbiology
Dmitrii Y. Y. Travin, Romain Jouan, Armelle Vigouroux, Satomi Inaba-Inoue, Joy Lachat, Fazal Haq, Tatiana Timchenko, Dmitry Sutormin, Svetlana Dubiley, Konstantinos Beis, Solange Morera, Konstantin Severinov, Peter Mergaert
Summary: The peptide antibiotic Phazolicin (PHZ) exhibits low resistance in Sinorhizobium meliloti due to its dual-entry mode through two different peptide transporters. This finding makes PHZ an attractive option for the development of biocontrol agents in agriculture.
Article
Microbiology
Jerome Trotereau, Romain Jouan, Delphine Naquin, Maxime Branger, Catherine Schouler, Philippe Velge, Peter Mergaert, Isabelle Virlogeux-Payant
Summary: In this study, a high-density transposon sequencing library of the Salmonella Typhimurium ATCC 14028 strain was constructed and characterized, and essential genes for growth in rich culture medium were identified.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Plant Sciences
Senlei Zhang, Ting Wang, Rui M. Lima, Aladar Pettko-Szandtner, Attila Kereszt, J. Allan Downie, Eva Kondorosi
Summary: Symbiotic nitrogen fixation by Rhizobium bacteria in legume root nodules alleviates the need for nitrogen fertilizers. Terminal differentiation of bacteroids is controlled by nodule-specific cysteine-rich (NCR) peptides, which are absent in legumes like soybean. The expression of NCR genes does not require NCR-specific transcription factors, but two AHL transcription factors found in Medicago truncatula and soybean nodules induce NCR gene expression. Mutation of NCR169 arrests bacteroid development, while the absence of MtAHL1 or MtAHL2 completely blocks bacteroid differentiation. These findings suggest the possibility of increasing nitrogen fixation efficiency in legumes lacking NCRs.
Article
Physics, Fluids & Plasmas
J. Bouvard, C. Douarche, P. Mergaert, H. Auradou, F. Moisy
Summary: In this study, the aerotactic response of Burkholderia contaminans bacteria was investigated by tracking bacteria and measuring local oxygen concentration. The findings indicate that the aerotactic velocity is proportional to the local oxygen concentration raised to the power of -2, consistent with existing models.
Article
Biotechnology & Applied Microbiology
Tsubasa Ohbayashi, Raynald Cossard, Gaelle Lextrait, Takahiro Hosokawa, Vincent Lesieur, Kazutaka Takeshita, Kanako Tago, Peter Mergaert, Yoshitomo Kikuchi
Summary: This study investigated the gut symbionts of the Western conifer seed bug and found that the specificity of the symbionts is influenced by the host rather than geography.
MICROBES AND ENVIRONMENTS
(2022)
Article
Microbiology
George C. DiCenzo, Lisa Cangioli, Quentin Nicoud, Janis H. T. Cheng, Matthew J. Blow, Nicole Shapiro, Tanja Woyke, Emanuele G. Biondi, Benoit Alunni, Alessio Mengoni, Peter Mergaert
Summary: DNA methylation plays important roles in immunity and cellular processes regulation in Ensifer bacteria, especially in nitrogen-fixing symbiosis with legumes. Six genome-wide methylated motifs were identified using single-molecule real-time sequencing, with five of them being strain-specific. The GANTC motif recognized by the cell cycle-regulated CcrM methyltransferase was methylated in all strains. The methylation of GANTC motifs increased progressively from ori to ter regions in actively dividing cell cultures, while near full genome-wide GANTC methylation was observed in the early stage of symbiotic differentiation, followed by a moderate decrease in overall methylation and a progressive decrease in chromosomal GANTC methylation in later stages of differentiation. These findings suggest dysregulated and constitutive CcrM activity during terminal differentiation may be a driving factor for endoreduplication of terminally differentiated bacteroids.