Article
Green & Sustainable Science & Technology
Zahwa A. Elsagan, Rehab M. Ali, Mohamed A. El -Naggar, E. -s. z. El-Ashtoukhy, Sara E. AbdElhafez
Summary: In Egypt, second-generation bioethanol production from agricultural waste is thriving as a response to the excessive usage caused by the Covid-19 pandemic. Lignocellulosic biomass, due to its abundance and renewability, shows great potential as a feedstock for bioethanol production. However, the challenge lies in achieving effective enzyme accessibility through functional delignification without impacting the cellulose matrices. This study focuses on a novel physio-chemical combination for corn stover pretreatment, resulting in the production of bioethanol through hydrolysis and fermentation processes.
Article
Agronomy
Iria Firvida, Pablo G. del Rio, Patricia Gullon, Beatriz Gullon, Gil Garrote, Aloia Romani
Summary: This study evaluated a delignification process using lime as an alternative alkali to improve enzymatic saccharification of corn stover cellulose, leading to successful production of bioethanol. Through experimental design, optimal conditions were identified for high cellulose and xylan recovery rates, delignification efficiency, and cellulose-to-glucose conversion rate.
Article
Agricultural Engineering
Kai Li, Cheng Li, Xin-Qing Zhao, Chen-Guang Liu, Feng-Wu Bai
Summary: Corynebacterium glutamicum was engineered to efficiently produce succinic acid from corn stover pre-treated by concentrated-alkali under steam-assistant conditions. The engineered strain achieved high succinate production by blocking by-products pathways, enhancing carbon flux, and releasing end-product inhibition. Xylose utilization pathways were also introduced into the strain for fully utilizing xylose in lignocellulosic hydrolysate. The combined use of glucose and xylose yielded 64.16 g/L of succinic acid from CASA-pretreated corn stover.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Jaewon Byun, Jeehoon Han
Summary: A rigorous life cycle assessment was conducted to analyze two different technological process configurations for producing bioethanol from corn stover, and the results showed that nonenzymatic chemical hydrolysis and subsequent fermentation (P2) is the most promising strategy in terms of reducing climate change, fossil depletion, and air pollutants.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Yuwei Zhang, Zhaoxian Xu, Minrui Lu, Xingwang Ma, Sitong Chen, Yanchen Wang, Wenyuan Shen, Pingping Li, Mingjie Jin
Summary: A lignocellulosic biomass-based lactic acid biorefinery system was developed in this study, which involved chemical pretreatment and densification of the biomass. The system successfully increased lactic acid titers, enabling efficient scale production of cellulose-based lactic acid.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Yinling Gao, Fengqin Wang, Xiaojuan Li, Guotao Mao, Hui Xie, Andong Song, Julio Cesar dos Santos, Zhanying Zhang
Summary: This study investigated the production of citric acid and mannitol by Yarrowia lipolytica CGMCC 2.1506 using glucose and glycerol as carbon sources. Optimal cultivation conditions were determined and applied to enzyme hydrolysates derived from glycerol-assisted instant catapult steam explosion (ICSE) without detoxification, glycerol removal, or nutrient addition. High concentrations of citric acid and mannitol were achieved, demonstrating the potential of this integrated process for biorefining of lignocellulosic biomass.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Agricultural Engineering
Xiaoqing Lin, Yao Liu, Xiaojie Zheng, Nasib Qureshi
Summary: The study developed a short time and low energy consumption pretreatment process using deep eutectic solvent (DES) to enhance cellulose recovery and total sugar released from corn stover, leading to a successful production of butanol by Clostridium beijerinckii P260 with high productivity and yield. This work provides insight into the efficient application of DES systems in lignocellulose biomass biorefinery.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Katarina Mihajlovski, Danijela Pecarski, Mirjana Rajilic-Stojanovic, Suzana Dimitrijevic-Brankovic
Summary: This study demonstrated the production of hydrolytic enzymes, lignocellulosic hydrolysis, and bioethanol production using agricultural wastes by Hymenobacter sp. CKS3. Optimized conditions led to high enzyme activity and reducing sugar yield, highlighting the potential of Hymenobacter for sustainable production of biofuels.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Agricultural Engineering
Cristina Alvarez, Alberto Gonzalez, Ignacio Ballesteros, Maria Jose Negro
Summary: Through steam explosion pretreatment of barley straw, different enzyme combinations were utilized for valorization of hemicellulose fraction to produce XOS. The solid fraction was then used for bioethanol production through presaccharification and simultaneous saccharification and fermentation process.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Applied
Anuj Thakkar, Katelyn M. Shell, Martino Bertosin, Dylan D. Rodene, Vinod Amar, Alberto Bertucco, Ram B. Gupta, Rajesh Shende, Sandeep Kumar
Summary: An integrated process for converting corn stover into levulinic acid and biocarbon electrode material was proposed, involving hydrothermal pretreatment and acid hydrolysis. This process resulted in the conversion of glucan to levulinic acid, while the remaining residue was converted into biocarbon for supercapacitor electrodes, demonstrating financial and environmental sustainability.
FUEL PROCESSING TECHNOLOGY
(2021)
Article
Agricultural Engineering
Hongqiong Zhang, Rui Zhang, Yunong Song, Xinying Miu, Quanguo Zhang, Jingbo Qu, Yong Sun
Summary: This study investigated the effects of using urea combined with steam explosion on the enzymatic hydrolysis and ethanol production of corn stover. The results showed that optimal conditions for ethanol production were 4.87% urea addition and 1.22 MPa steam pressure. The addition of urea and steam explosion significantly increased the reducing sugar yield and degradation rates of cellulose, hemicellulose, and lignin.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Physical
Haruhiko Teramoto, Masako Suda, Masayuki Inui
Summary: This study investigates the inhibitory compounds in dilute sulfuric acid-pretreated corn stover on hydrogen production by Escherichia coli. The results indicate that acetate is the primary inhibitory factor.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Engineering, Environmental
Maria Jose Fernandez-Rodriguez, Maryam Mushtaq, Li Tian, Antonia Jimenez-Rodriguez, Barbara Rincon, Brandon H. Gilroyed, Rafael Borja
Summary: Lignocellulosic by-products from agricultural crops are important raw materials for anaerobic digestion and clean renewable energy. Different physicochemical pretreatments were compared using corn stover biomass, with alkaline pretreatment leading to the highest methane yield and autoclave pretreatment resulting in the highest organic matter solubilization. Mathematical models were utilized to assess anaerobic biodegradation, showing an increase in kinetic constants with pretreatment methods.
WASTE MANAGEMENT & RESEARCH
(2022)
Article
Thermodynamics
Xinchuan Yuan, Guannan Shen, Sitong Chen, Xiangxue Chen, Chengcheng Zhang, Shuangmei Liu, Mingjie Jin
Summary: Corn stover pretreated by DLC exhibited higher digestibility and fermentability compared to traditional pre-treatments. However, the xylose fermentation during SHCF was unsatisfactory at high solid loadings. In this study, modified SSCF was adopted to promote xylose utilization and ethanol yield. A novel strategy, Fed-batch of DLC Biomass and Enzyme (FBE), showed higher sugar conversion, xylose consumption, and superior ethanol yield compared to SHCF and traditional SSCF.
Article
Chemistry, Applied
David B. Johnston, Nhuan P. Nghiem
Summary: The study demonstrates the efficient co-fermentation of pretreated biomass with corn to produce cellulosic ethanol, with yields similar to those from biomass alone. This method eliminates many economic and processing issues faced by standalone cellulosic ethanol facilities.
Article
Chemistry, Physical
Nicola Di Fidio, Anna Maria Raspolli Galletti, Sara Fulignati, Domenico Licursi, Federico Liuzzi, Isabella De Bari, Claudia Antonetti
Article
Agricultural Engineering
Nicola Di Fidio, Sara Fulignati, Isabella De Bari, Claudia Antonetti, Anna Maria Raspolli Galletti
BIORESOURCE TECHNOLOGY
(2020)
Article
Energy & Fuels
Elisabetta Borsella, Isabella De Bari, Pietro Colucci, Silvio Mastrolitti, Federico Liuzzi, Adriana De Stefanis, Veronica Valentini, Fortunata Gallese, Giorgio Perez
Article
Agricultural Engineering
Antonio Caporusso, Isabella De Bari, Vito Valerio, Roberto Albergo, Federico Liuzzi
Summary: This study investigated the production of Single Cell Oils from undetoxified cardoon hydrolysates, with Lipomyces tetrasporus showing better tolerance to the toxic effects of biomass degradation by-products compared to Cutaneotrichosporon curvatus. In batch mode using SP-inocula and the highest dilution ratio, the maximum lipids yields and lipid cell content were achieved, with oleic acid being the dominant fatty acid. Scaling up to the 2 L bioreactor in fed-batch mode increased lipids concentration and productivity.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Engineering, Chemical
Isabella De Bari, Federico Liuzzi, Alfredo Ambrico, Mario Trupo
Review
Engineering, Chemical
Cosetta Ciliberti, Antonino Biundo, Roberto Albergo, Gennaro Agrimi, Giacobbe Braccio, Isabella de Bari, Isabella Pisano
Article
Engineering, Chemical
Isabella De Bari, Aristide Giuliano, Maria Teresa Petrone, Giovanni Stoppiello, Vittoria Fatta, Cecilia Giardi, Francesco Razza, Alessandra Novelli
Article
Green & Sustainable Science & Technology
Luigi Pari, Vincenzo Alfano, Walter Stefanoni, Francesco Latterini, Federico Liuzzi, Isabella De Bari, Vito Valerio, Anna Ciancolini
Summary: Cardoon roots are a suitable source of inulin for producing biodegradable bioplastics, and findings from a storage trial suggest that pre-processing roots before storage can reduce drying time and costs, while increasing bulk density without affecting inulin content. Developing a sustainable supply chain for inulin production from cardoon roots is crucial for cost efficiency and quantity optimization.
Article
Energy & Fuels
Donatella Danzi, Ivana Marino, Isabella De Bari, Silvio Mastrolitti, Giacomo L. Petretto, Domenico Pignone, Michela Janni, Francesco Cellini, Tullio Venditti
Summary: The study analyzed the digestibility of wheat straw and found that lignin content is the major factor affecting enzymatic hydrolysis. The possible role of other cell wall components was also discussed.
Article
Biotechnology & Applied Microbiology
Antonio Caporusso, Isabella De Bari, Aristide Giuliano, Federico Liuzzi, Roberto Albergo, Rocchina Pietrafesa, Gabriella Siesto, Assunta Romanelli, Giacobbe Braccio, Angela Capece
Summary: Microbial lipids derived from wheat straw were produced by Lipomyces tetrasporus DSM 70314 through steam explosion and enzymatic hydrolysis. The enzymatic hydrolysis conditions were optimized using response surface methodology, and the nitrogen source and carbon-to-nitrogen ratio of the fermentation medium were found to be soy flour and 160, respectively. Under optimized conditions, a lipid concentration of 14.8 g/L, a lipid yield of 23.1%, and a lipid cell content of 67.4% were achieved.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Antonio Caporusso, Angela Capece, Isabella De Bari
Summary: The agri-food industry annually produces a large amount of crop residues and wastes, and the proper management of these products is crucial to enhance the sustainability of agro-industrial production. Oleaginous yeasts serve as versatile tools for producing biobased chemicals using agri-food wastes as feedstocks.
FERMENTATION-BASEL
(2021)
Article
Agricultural Engineering
Nicola Di Fidio, Federico Dragoni, Claudia Antonetti, Isabella De Bari, Anna Maria Raspolli Galletti, Giorgio Ragaglini
BIORESOURCE TECHNOLOGY
(2020)