Article
Green & Sustainable Science & Technology
Knawang Chhunji Sherpa, Debajyoti Kundu, Subhodeep Banerjee, Makarand Madhao Ghangrekar, Rintu Banerjee
Summary: This study explores the potential of using sugarcane tops as a feedstock for ethanol production and conducts experiments using different fermentation approaches. The results show that simultaneous saccharification and fermentation (SSF) is more efficient than separate fermentation, and partially consolidated bioprocessing (PCBP) further improves fermentation efficiency.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Biotechnology & Applied Microbiology
Alison Andrei Schmatz, Joao Paulo Candido, Dejanira de Franceschi de Angelis, Michel Brienzo
Summary: This study evaluated the impact of dilute acid pretreatment on the hydrolysis and fermentation of sugarcane bagasse. The addition of an antioxidant resulted in lower levels of inhibitors, and partially delignified material showed improved cellulose solubility and ethanol production.
FERMENTATION-BASEL
(2023)
Article
Energy & Fuels
Rajnish Kumar, Om Prakash
Summary: Researchers optimized the fermentation conditions for bioethanol production from wheat-stalk straw in different seasons with minimal laboratory resources. It was found that the maximum bioethanol yield varied in different seasons, with the highest yield in the monsoon season. The enhancement in maximum bioethanol yield in the monsoon season was 8.42% and 12.57% compared to the summer and spring seasons, respectively.
Article
Energy & Fuels
Lucia Coimbra, Karen Malan, Alejandra Fagundez, Mairan Guigou, Claudia Lareo, Belen Fernandez, Martin Pratto, Silvia Batista
Summary: Genetically engineered yeast strains were developed for efficient ethanol production from lignocellulosic biomass. The recombinant strain CAT-1-XIT (pRS42K::XI) showed promising results with 74% consumption of D-xylose and production of 12.6 g/L ethanol.
BIOENERGY RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Luca Ricciardi, Willem Verboom, Jean-Paul Lange, Jurriaan Huskens
Summary: This study demonstrates an effective conversion of xylose into furfural with a selectivity of over 90% using a three-solvent system. The use of an apolar aromatic solvent, a polar organic solvent, and acidic water catalyzes the formation of furfural. The transition of the three-solvent system from biphasic to monophasic conditions under reaction conditions is crucial for achieving high selectivity.
Article
Chemistry, Multidisciplinary
Kenkichi Taniguchi, Etty N. Kusumawati, Hidetaka Nanao, Chandrashekhar V. Rode, Osamu Sato, Aritomo Yamaguchi, Masayuki Shirai
Summary: Solvolysis of benzyl phenyl ether (BPE) in an aqueous ethanol solution under high-pressure carbon dioxide conditions resulted in the production of monoaromatics. The ethanol molar fraction was found to be the key factor for the solvolysis reaction, and the highest monoaromatic yield of 72.9% was obtained in a water:ethanol solution (2 cm³ : 1 cm³) at 598 K for 3 hours under a pressure of 18 MPa of carbon dioxide. Continuous production of monoaromatics was achieved through a flow system, with a yield of 76.7%.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Afrasiab Khan Tareen, Vittaya Punsuvon, Imrana Niaz Sultan, Muhammad Waseem Khan, Pramuk Parakulsuksatid
Summary: The study focused on enhancing ethanol production through investigating high substrate loading, enzyme and substrate feeding strategies, and pre-hydrolysis effects. The results showed that adding enzyme at the start of fed-batch SSF significantly increased ethanol concentration, while PSSF with substrate feeding improved enzyme hydrolysis efficiency and ethanol yields. These findings suggest that optimizing enzyme and substrate feeding strategies can enhance ethanol production in a SSF process.
Article
Microbiology
Haijie Wang, Limin Cao, Qi Li, Nalin N. Wijayawardene, Jian Zhao, Min Cheng, Qi-Rui Li, Xiaobin Li, Itthayakorn Promputtha, Ying-Qian Kang
Summary: Efficient renewable bioethanol can alleviate energy crisis and environmental pollution. Genetically modified strain GRE3(OE) shows remarkable fermentation efficiency in different lignocellulosic hydrolysates, making it a promising candidate for industrial ethanol development.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Energy & Fuels
Suncica Beluhan, Katarina Mihajlovski, Bozidar Santek, Mirela Ivancic Santek
Summary: Bioethanol is a widely used alternative transportation fuel that is considered clean, renewable, and environmentally friendly. This review provides an overview of the steps and challenges involved in producing bioethanol from lignocellulosic biomass. It discusses pretreatment methods, process strategies, strain development, ethanol isolation and purification, and technical hurdles.
Article
Biotechnology & Applied Microbiology
Hironaga Akita, Tetsuya Goshima, Toshihiro Suzuki, Yuya Itoiri, Zen-ichiro Kimura, Akinori Matsushika
Summary: Simultaneous saccharification and fermentation (SSF) offers advantages in biofuel production by simplifying the manufacturing process, operating easily, and reducing energy input. Using Pichia kudriavzevii NBRC1279 and NBRC1664 in SSF for bioethanol production resulted in high ethanol concentrations without the need for pretreatment of the source material, making it suitable for industrial use. Genome sequencing and comparison revealed similar functional abilities between the strains, but slight differences in stress tolerance, particularly in strain NBRC1279.
FERMENTATION-BASEL
(2021)
Article
Agricultural Engineering
Meilin Kong, Xiaowei Li, Tongtong Li, Xuebing Zhao, Mingjie Jin, Xin Zhou, Hanqi Gu, Vladimir Mrsa, Wei Xiao, Limin Cao
Summary: CCW12(OE) is a Saccharomyces cerevisiae strain constructed by overexpressing CCW12, which shows high ethanol production efficiency in various hydrolysates and exhibits better stress resistance and fermentation performance compared to the control strain WXY70.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Catia V. T. Mendes, Jorge M. S. Rocha, M. Graca V. S. Carvalho
Summary: This study demonstrated the production of bioethanol from lignocellulosic residue through simultaneous saccharification and fermentation. Increasing solid content improved the ethanol concentration, yield, and productivity, but a decrease was observed at the maximum solid concentration. The co-application of two enzyme complexes positively affected the conversion efficiency.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Renan Yuji Miyamoto, Ricardo Rodrigues de Melo, Isabelle Lobo de Mesquita Sampaio, Amanda Silva de Sousa, Edvaldo Rodrigo Morais, Cintia Regina Sargo, Leticia Maria Zanphorlin
Summary: Isomerases, particularly xylose isomerases (XIs), are enzymes widely studied for their role in industrial processes such as biotransforming xylose. The application of XIs in downstream metabolism of microorganisms is a smart strategy for producing value-added biochemicals with broad industrial applications. Recent advancements in XIs have expanded their range of applications, providing comprehensive insights into their mechanisms of action and potential for developing xylose bioprocesses.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Agricultural Engineering
Yuwei Zhang, Zhaoxian Xu, Minrui Lu, Xingwang Ma, Sitong Chen, Yanchen Wang, Wenyuan Shen, Pingping Li, Mingjie Jin
Summary: A lignocellulosic biomass-based lactic acid biorefinery system was developed in this study, which involved chemical pretreatment and densification of the biomass. The system successfully increased lactic acid titers, enabling efficient scale production of cellulose-based lactic acid.
BIORESOURCE TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Ajay Kumar Pandey, Mohit Kumar, Sonam Kumari, Naseem A. Gaur
Summary: In this study, paddy straw hydrolysate (PSH) was integrated with molasses as a diluent for ethanol production using a robust Saccharomyces cerevisiae NGY10 strain. The integration of PSH as a diluent not only increased ethanol yield and productivities but also saved water consumption and operational costs, and could be accommodated in existing sugar mill infrastructures.
Article
Chemistry, Physical
Nicola Di Fidio, Anna Maria Raspolli Galletti, Sara Fulignati, Domenico Licursi, Federico Liuzzi, Isabella De Bari, Claudia Antonetti
Article
Microbiology
Alfredo Ambrico, Mario Trupo, Rosaria Magarelli, Roberto Balducchi, Angelo Ferraro, Evangelos Hristoforou, Tiziana Marino, Dino Musmarra, Patrizia Casella, Antonio Molino
Article
Agricultural Engineering
Nicola Di Fidio, Sara Fulignati, Isabella De Bari, Claudia Antonetti, Anna Maria Raspolli Galletti
BIORESOURCE TECHNOLOGY
(2020)
Article
Energy & Fuels
Elisabetta Borsella, Isabella De Bari, Pietro Colucci, Silvio Mastrolitti, Federico Liuzzi, Adriana De Stefanis, Veronica Valentini, Fortunata Gallese, Giorgio Perez
Article
Agricultural Engineering
Antonio Caporusso, Isabella De Bari, Vito Valerio, Roberto Albergo, Federico Liuzzi
Summary: This study investigated the production of Single Cell Oils from undetoxified cardoon hydrolysates, with Lipomyces tetrasporus showing better tolerance to the toxic effects of biomass degradation by-products compared to Cutaneotrichosporon curvatus. In batch mode using SP-inocula and the highest dilution ratio, the maximum lipids yields and lipid cell content were achieved, with oleic acid being the dominant fatty acid. Scaling up to the 2 L bioreactor in fed-batch mode increased lipids concentration and productivity.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Green & Sustainable Science & Technology
Luigi Pari, Vincenzo Alfano, Walter Stefanoni, Francesco Latterini, Federico Liuzzi, Isabella De Bari, Vito Valerio, Anna Ciancolini
Summary: Cardoon roots are a suitable source of inulin for producing biodegradable bioplastics, and findings from a storage trial suggest that pre-processing roots before storage can reduce drying time and costs, while increasing bulk density without affecting inulin content. Developing a sustainable supply chain for inulin production from cardoon roots is crucial for cost efficiency and quantity optimization.
Article
Energy & Fuels
Donatella Danzi, Ivana Marino, Isabella De Bari, Silvio Mastrolitti, Giacomo L. Petretto, Domenico Pignone, Michela Janni, Francesco Cellini, Tullio Venditti
Summary: The study analyzed the digestibility of wheat straw and found that lignin content is the major factor affecting enzymatic hydrolysis. The possible role of other cell wall components was also discussed.
Article
Biotechnology & Applied Microbiology
Antonio Caporusso, Isabella De Bari, Aristide Giuliano, Federico Liuzzi, Roberto Albergo, Rocchina Pietrafesa, Gabriella Siesto, Assunta Romanelli, Giacobbe Braccio, Angela Capece
Summary: Microbial lipids derived from wheat straw were produced by Lipomyces tetrasporus DSM 70314 through steam explosion and enzymatic hydrolysis. The enzymatic hydrolysis conditions were optimized using response surface methodology, and the nitrogen source and carbon-to-nitrogen ratio of the fermentation medium were found to be soy flour and 160, respectively. Under optimized conditions, a lipid concentration of 14.8 g/L, a lipid yield of 23.1%, and a lipid cell content of 67.4% were achieved.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Rosaria Alessandra Magarelli, Mario Trupo, Alfredo Ambrico, Vincenzo Larocca, Maria Martino, Salvatore Palazzo, Roberto Balducchi, Vesa Joutsjoki, Anne Pihlanto, Annamaria Bevivino
Summary: This study evaluated the use of prickly pear pruning waste as a raw material for the production of Plant Growth Promoting Microorganisms (PGPMs). The researchers successfully cultivated a synthetic microbial consortium in a laboratory and pilot scale, using the juice from Opuntia cladodes as a culture medium. The results showed that the bacteria grown in the cladodes juice media had higher survival rates after air-drying or freeze-drying compared to those grown in conventional media.
FERMENTATION-BASEL
(2022)
Review
Biotechnology & Applied Microbiology
Antonio Caporusso, Angela Capece, Isabella De Bari
Summary: The agri-food industry annually produces a large amount of crop residues and wastes, and the proper management of these products is crucial to enhance the sustainability of agro-industrial production. Oleaginous yeasts serve as versatile tools for producing biobased chemicals using agri-food wastes as feedstocks.
FERMENTATION-BASEL
(2021)