Article
Cell & Tissue Engineering
Stephanie Merfeld-Clauss, Hongyan Lu, Keith L. March, Dmitry O. Traktuev
Summary: Vascularization is crucial for tissue repair and replacement therapies. Endothelial cells (ECs) and mesenchymal stem/stromal cells (MSCs) can spontaneously organize into vessels after coimplantation. Notch signaling pathways play a role in regulating activin A expression in EC-adipose mesenchymal stromal cells (ASC) cocultures. Interrupted notch signaling inhibits activin A production, but silencing Notch2 and Notch3 produce opposing effects on ASC maturation and mural cell marker expression.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Jaana Schneider, Marianne Pultar, Johannes Oesterreicher, Madhusudhan Reddy Bobbili, Severin Muhleder, Eleni Priglinger, Heinz Redl, Andreas Spittler, Johannes Grillari, Wolfgang Holnthoner
Summary: EVs did not mediate RNA transmission between ASC and EC in coculture systems, suggesting that Cre mRNA may not be packaged into EVs or EVs may not be taken up by the utilized cell types. Further investigations are needed to develop a system that efficiently and specifically loads Cre mRNA or protein into EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Evan Paul Tracy, Michaela Dukes, Gabrielle Rowe, Jason E. Beare, Rajeev Nair, Amanda Jo LeBlanc
Summary: This study aimed to investigate the mechanisms underlying the restorative effects of adipose stromal vascular fraction (SVF) on vasodilation in aging-induced coronary microvascular disease (CMD). The results showed that SVF can attenuate chronic accumulation of reactive oxygen species (ROS) and enhance antioxidant gene and protein expression in coronary arterioles, thus restoring flow- and beta 1-adrenergic receptor (beta 1ADR)-mediated vasodilation. The study also identified a novel mechanism involving the impact of ROS on beta 1ADR trafficking.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Alina Chelmus-Burlacu, Eric Tang, Dragos Pieptu
Summary: This study evaluated the effects of chlorhexidine and povidone-iodine on cellular constructs and found that povidone-iodine had a greater inhibitory effect on cell migration and sprouting, although it had minimal effects at low concentrations.
Article
Medicine, Research & Experimental
L. B. Buravkova, M. Ezdakova, I. Andrianova, A. N. Gornostaeva, P. Bobyleva, E. R. Andreeva
Summary: The study demonstrated that during ex vivo expansion, cord blood hematopoietic stem progenitor cells (cbHSPCs) can influence the functional state of mesenchymal stromal cells (MSCs), leading to the formation of early stromal progenitors with a bipotential osteo-adipogenic profile. The joint coculture resulted in a specific hematopoiesis-supportive environment, with the expression of certain MSC genes upregulated and a balance of myeloid regulators maintained. Short-term osteoinduction prior to coculture enhanced the development of a bipotential profile, indicating a more committed state of cbHSPCs.
Article
Cell & Tissue Engineering
Abid Hussain, Virginie Deleuze, Leila El Kebriti, Hulya Turali, Nelly Pirot, Yael Glasson, Daniele Mathieu, Valerie Pinet
Summary: Lyl1 deficiency in mice leads to mild hematopoietic and vascular defects. Interestingly, Lyl1(-/-) mice exhibit transient overweight due to early differentiation of adipose stem cells into adipocytes, driven by an immature vascular niche in the adipose tissue. The impaired vascular compartment in Lyl1(-/-) mice promotes ASC differentiation, leading to premature adipocyte expansion and ASC depletion.
Article
Surgery
Fuke Wang, Guiran Yang, Yu Xiao, Chuan He, Guofeng Cai, En Song, Yanlin Li
Summary: The study aimed to investigate the effects of tissue-engineered bone constructed with PDPBB and coculture of ADSCs and VECs on host immune status. The results showed that there was no significant immune rejection after transplantation of tissue-engineered bone. Changes in T-lymphocyte subpopulations varied among different groups but did not indicate immune rejection.
ANNALS OF PLASTIC SURGERY
(2021)
Article
Cell Biology
Maria Serena Piccinno, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, Ilenia Mastrolia, Chiara Chiavelli, Ilaria Castelli, Daniela Bernabei, Mara Tagliazucchi, Elisa Bonetti, Francesca Lolli, Giorgio De Santis, Massimo Dominici, Elena Veronesi
Summary: This study investigates the use of adipose-derived mesenchymal stromal/stem cells (AD-MSCs) in autologous fat transfer (AFT) for tissue reconstruction. The results show that AD-MSCs can improve graft persistence and reduce tissue resorption after AFT. The implanted AD-MSCs can persist and differentiate into mature adipocytes, leading to better outcomes in terms of graft weight and reduced cyst formation. This suggests a long-term protective and anti-inflammatory/proangiogenic role of AD-MSCs in autologous AFT tissue resorption.
Article
Oncology
Maiko Sasaki, Yoonhee Jung, Paula North, Justin Elsey, Keith Choate, Michael Andrew Toussaint, Christina Huang, Rakan Radi, Adam J. Perricone, Victor G. Corces, Jack L. Arbiser
Summary: Mutations in GNAQ lead to vascular malformations, and introducing mutant GNAQ into murine endothelial cells resulted in progressive vascular malformations. The upregulation of C-Kit in the endothelial cells and its potential as a druggable target for Sturge-Weber syndrome were identified. Imatinib, an FDA-approved drug targeting C-Kit, showed effectiveness in reducing the size of vascular malformations in vivo.
Article
Cell & Tissue Engineering
Julien Guerrero, Boris Dasen, Agne Frismantiene, Sebastien Pigeot, Tarek Ismail, Dirk J. Schaefer, Maria Philippova, Therese J. Resink, Ivan Martin, Arnaud Scherberich
Summary: The presence of T-cadherin-positive cells derived from the stromal vascular fraction (SVF) is essential for the osteogenic and vasculogenic properties of SVF cells. The crosstalk between stromal cells and T-cadherin-expressing endothelial cells within adipose tissue critically regulates osteogenesis.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Cell Biology
S. Gandolfi, B. Pileyre, L. Drouot, I. Dubus, I. Auquit-Auckbur, J. Martinet
Summary: Muscle regeneration is a complex process that requires precise interaction between immune and muscular cells. The mechanisms underlying autoimmune myopathies are not fully understood, but chronic inflammation and decreased muscle regenerative capacity play a role. Immunosuppressive treatments can be ineffective, and alternative therapies such as stromal vascular fraction show promise but lack a clear understanding of their active components and mechanisms of action.
CELL DEATH DISCOVERY
(2023)
Article
Surgery
Kevin M. Urlaub, Kavitha Ranganathan, Jeremy V. Lynn, Alexandra O. Luby, Lauren N. Patrick, Noah S. Nelson, Alexis Donneys, Steven R. Buchman
Summary: Stromal vascular fraction therapy has shown significant efficacy in enhancing bone healing in irradiated fracture repair, improving union rates and bone vascularization metrics while restoring bone cellularity and maturity despite preoperative irradiation. This study provides a promising foundation for the development of noncultured, cell-based therapies for clinical implementation.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2021)
Review
Orthopedics
Kang-Il Kim, Myung-Seo Kim, Jun-Ho Kim
Summary: This study conducted a systematic review and meta-analysis to evaluate the efficacy and safety of intra-articular injections of autologous adipose-derived stem cells (ASCs) and adipose-derived stromal vascular fractions (ADSVFs) in patients with knee osteoarthritis (OA). The results showed that this treatment approach showed significant clinical efficacy and safety in terms of pain relief and functional improvement at short-term follow-up. However, the evidence for cartilage regeneration remains limited.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2023)
Article
Medicine, General & Internal
Lucy V. Trevor, Kirsten Riches-Suman, Ajay L. Mahajan, M. Julie Thornton
Summary: This study aims to isolate stromal vascular fraction from human breast tissue exposed to radiotherapy and determine the presence of adipose-derived stem cells. The results show that stromal vascular fraction from irradiated donors has a similar effect in stimulating the migration of dermal fibroblasts as pre-adipocyte conditioned media from healthy donors. Thus, the functionality of adipose-derived stem cells appears to be preserved following radiotherapy.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Alexandra M. Roch, Thomas K. Maatman, Todd G. Cook, Howard H. Wu, Stephanie Merfeld-Clauss, Dmitry O. Traktuev, Keith L. March, Nicholas J. Zyromski
JOURNAL OF GASTROINTESTINAL SURGERY
(2020)
Article
Medicine, General & Internal
Yameena Jawed, Eleni Beli, Keith March, Anthony Kaleth, M. Terry Loghmani
Article
Cell & Tissue Engineering
Ricardo L. Rodriguez, Trivia Frazier, Bruce A. Bunnell, Cecilia A. Mouton, Keith L. March, Adam J. Katz, J. Peter Rubin, Ramon Llull, Jens A. Sorensen, Jeffrey M. Gimble
STEM CELLS AND DEVELOPMENT
(2020)
Editorial Material
Cell & Tissue Engineering
J. Peter Rubin, Geoffrey C. Gurtner, Wei Liu, Keith L. March, Riitta Seppanen-Kaijansinkko, Michael J. Yaszemski, James J. Yoo
TISSUE ENGINEERING PART A
(2020)
Review
Medicine, General & Internal
Ruxandra Sava, Carl J. Pepine, Keith L. March
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Medicine, Research & Experimental
Amanda Lindeman, Carl J. Pepine, Keith L. March
JOURNAL OF TRANSLATIONAL MEDICINE
(2020)
Article
Cardiac & Cardiovascular Systems
Meijing Wang, Liangliang Yan, Qianzhen Li, Yang Yang, Mark Turrentine, Keith March, I-wen Wang
Summary: This study aimed to evaluate the effectiveness of MSC-conditioned medium (CM) and extracellular vesicles (EVs) in improving ischemia/reperfusion-induced myocardial damage in donor hearts. The results showed that MSC-CM and MSC-EVs can provide better myocardial preservation during prolonged cold static storage in donor hearts. Additionally, MSC-EVs can be used for intercellular transport of miRNAs in heart transplantation.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2022)
Article
Cell Biology
Hongyan Lu, Stephanie Merfeld-Clauss, Yameena Jawed, Keith L. March, Michael E. Coleman, Natalia V. Bogatcheva
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Cardiac & Cardiovascular Systems
Roberto Bolli, Raul D. Mitrani, Joshua M. Hare, Carl J. Pepine, Emerson C. Perin, James T. Willerson, Jay H. Traverse, Timothy D. Henry, Phillip C. Yang, Michael P. Murphy, Keith L. March, Ivonne H. Schulman, Sohail Ikram, David P. Lee, Connor O'Brien, Joao A. Lima, Mohammad R. Ostovaneh, Bharath Ambale-Venkatesh, Gregory Lewis, Aisha Khan, Ketty Bacallao, Krystalenia Valasaki, Bangon Longsomboon, Adrian P. Gee, Sara Richman, Doris A. Taylor, Dejian Lai, Shelly L. Sayre, Judy Bettencourt, RachelW. Vojvodic, Michelle L. Cohen, Lara Simpson, David Aguilar, Catalin Loghin, Lem Moye, Ray F. Ebert, Barry R. Davis, Robert D. Simari
Summary: The CONCERT-HF trial demonstrated the safety and feasibility of CPCs and MSCs treatment in ischemic cardiomyopathy-induced heart failure patients, with CPCs reducing MACE and MSCs improving quality of life. Further investigation is warranted to explore the potential beneficial effects of these cell types in heart failure.
EUROPEAN JOURNAL OF HEART FAILURE
(2021)
Article
Cardiac & Cardiovascular Systems
Susan R. Scott, Keith L. March, I-Wen Wang, Kanhaiya Singh, Jianyun Liu, Mark Turrentine, Chandan K. Sen, Meijing Wang
Summary: The study showed that using MSC secretome (particularly from bone marrow MSCs and adipose-MSCs) during prolonged cold storage preserves the normal transcriptional fingerprint, improves myocardial function depression, reduces inflammatory cytokine production, decreases apoptosis, and reduces myocardial damage, suggesting the synergistic protective effects of MSC-released soluble factors and exosomes on donor hearts.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Oncology
Nida Waheed, Michael G. Fradley, David L. DeRemer, Ahmad Mahmoud, Chintan P. Shah, Taimour Y. Langaee, Gloria P. Lipori, Keith March, Carl J. Pepine, Rhonda M. Cooper-DeHoff, Yonghui Wu, Yan Gong
Summary: This study indicates a high incidence of newly diagnosed cardiovascular disease after initiation of ICI therapy in a real-world clinical setting. Most incidents of cardiovascular disease were diagnosed shortly after initial exposure to ICIs, with some patients experiencing cardiotoxic effects leading to higher mortality rates.
Article
Biochemical Research Methods
Xiang Ren, Bradley W. Ellis, George Ronan, Stuart Ryan Blood, Cameron DeShetler, Satyajyoti Senapati, Keith L. March, Eileen Handberg, David Anderson, Carl Pepine, Hsueh-Chia Chang, Pinar Zorlutuna
Summary: The research has developed a miRNA detection platform for rapid diagnosis of acute myocardial infarction, which can increase detection sensitivity and reduce detection time. The expression levels of miR-1 show significant differences among different types of cardiac patients, helping to differentiate between various heart conditions.
Article
Cell & Tissue Engineering
Bradley W. Ellis, Dmitry O. Traktuev, Stephanie Merfeld-Clauss, Uryan Isik Can, Meijing Wang, Ray Bergeron, Pinar Zorlutuna, Keith L. March
Summary: Heart transplantation is a life-saving therapy for end-stage organ failure, but organ deterioration during transportation limits the number of hearts available for transplantation. Studies show that adipose-derived stem/stromal cell secretome (ASC-S) can ameliorate organ damage and increase the pool of hearts acceptable for transplantation.
Article
Oncology
Roberto Bolli, Emerson C. Perin, James T. Willerson, Phillip C. Yang, Jay H. Traverse, Timothy D. Henry, Carl J. Pepine, Raul D. Mitrani, Joshua M. Hare, Michael P. Murphy, Keith L. March, Sohail Ikram, David P. Lee, Connor O'Brien, Jean-Bernard Durand, Kathy Miller, Joao A. Lima, Mohammad R. Ostovaneh, Bharath Ambale-Venkatesh, Adrian P. Gee, Sara Richman, Doris A. Taylor, Shelly L. Sayre, Judy Bettencourt, Rachel W. Vojvodic, Michelle L. Cohen, Lara M. Simpson, Dejian Lai, David Aguilar, Catalin Loghin, Lem Moye, Ray F. Ebert, Barry R. Davis, Robert D. Simari
JACC: CARDIOONCOLOGY
(2020)