Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 137, Issue -, Pages 1403-1408Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2014.09.002
Keywords
Near-infrared spectroscopy; Partial least squares regression; High performance liquid chromatography; Kudzu
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Funding
- Major Projects of Independent Innovation Achievements of Shandong Province [2010ZDZX1A0406]
- Shandong Province Natural Science Foundation [ZR2011HM080]
- Wohua Pharmaceutical Co., Ltd.
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Near-infrared (NIR) spectroscopy has been developed into an indispensable tool for both academic research and industrial quality control in a wide field of applications. The feasibility of NIR spectroscopy to monitor the concentration of puerarin, daidzin, daidzein and total isoflavonoid (TIF) during the extraction process of kudzu (Pueraria lobata) was verified in this work NIR spectra were collected in transmission mode and pretreated with smoothing and derivative. Partial least square regression (PLSR) was used to establish calibration models. Three different variable selection methods, including correlation coefficient method, interval partial least squares (iPLS), and successive projections algorithm (SPA) were performed and compared with models based on all of the variables. The results showed that the approach was very efficient and environmentally friendly for rapid determination of the four quality indices (Qls) in the kudzu extraction process. This method established may have the potential to be used as a process analytical technological (PAT) tool in the future, (C) 2014 Elsevier B.V. All rights reserved.
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