Article
Cell Biology
Mohamed Chamlali, Sana Kouba, Lise Rodat-Despoix, Luca Matteo Todesca, Zoltan Pethoe, Albrecht Schwab, Halima Ouadid-Ahidouch
Summary: Orai3 regulates breast cancer cell migration through a Ca2+-dependent modulation of cell adhesive capacities and a Ca2+-independent regulation of the actin cytoskeleton via focal adhesion kinase (FAK).
Article
Cell Biology
Carsten Baltes, Divyendu Goud Thalla, Uli Kazmaier, Franziska Lautenschlaeger
Summary: Actin plays a crucial role in maintaining cell shape and generating forces. By stabilizing actin filaments with miuraenamide A, we observed a decrease in filament dynamics and an elongation of filament length, resulting in altered cell migration speed and nuclear position.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jianjiang Hu, Xiaowei Gong, Staffan Stromblad
Summary: Cells probe their microenvironment using membrane protrusion-retraction cycles. Spatial and temporal coordination between Rac1 and RhoA GTP-binding activities play critical roles in initiating and reinforcing protrusions and retractions. Rac1-GTP levels increase before protrusions, while RhoA-GTP levels increase before retractions. Rac1-GTP nadirs and peaks coincide with maximal edge velocity of local membrane protrusions and retractions, followed by declined edge velocity. The alteration of local Rac1-GTP precedes the alteration of traction force. Through optogenetic Rac1-GTP perturbations, we demonstrate the fundamental role of Rac1 in restricting the size and durability of protrusions and retractions, possibly through controlling traction forces.
Review
Biochemistry & Molecular Biology
Nayden G. Naydenov, Jennifer E. Koblinski, Andrei I. Ivanov
Summary: The scaffolding protein Anillin interacts with major cytoskeletal structures and plays a key role in regulating cell division and other important activities in non-dividing cells. Its overexpression is associated with poor prognosis in solid cancers, and it is implicated in the regulation of tumor growth and metastasis through both canonical and novel functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Eleonora Sarantelli, Apostolis Mourkakis, Lefteris C. Zacharia, Andreas Stylianou, Vasiliki Gkretsi
Summary: As metastasis is a major cause of cancer-related deaths, understanding the cellular and molecular events involved in cancer cell migration and invasion is crucial for developing novel anti-metastatic therapies. Fascin-1, an actin-bundling protein, plays a fundamental role in cell migration processes. It is significantly elevated in most cancers and its high expression is associated with aggressive disease and poor prognosis. Recent studies show that Fascin-1 is critically involved in metastasis and suggest it as a promising target for anti-metastatic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Laura Bozal-Basterra, Maria Gonzalez-Santamarta, Veronica Muratore, Natalia Martin-Martin, Amaia Ercilla, Jose A. Rodriguez, Arkaitz Carracedo, James D. Sutherland, Rosa Barrio
Summary: LUZP1 exhibits frequent genomic aberrations in cancer, particularly gene deletions, and its loss promotes cell migration, invasion, apoptosis, and alterations in cell morphology. Additionally, LUZP1 plays regulatory roles beyond actin filament bundling, affecting actin polymerization through changes in ACTR3 and phospho-cofilin ratios. These findings suggest a novel role for LUZP1 in cancer regulation through actin cytoskeleton control.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Frederic Larbret, Pierric Biber, Nicholas Dubois, Stoyan Ivanov, Laurence Lafanechere, Sophie Tartare-Deckert, Marcel Deckert
Summary: In this study, the Actin CytoFRET method was used to screen PTM-interfering compounds, and small molecule inhibitors of DUBs were found to induce actin polymerization and block cell migration. Further mechanistic investigation revealed that ROS-dependent cofilin modulation plays a key role in the regulation of actin dynamics.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Alecia Biel, Morgan Moser, Norman R. Groves, Iris Meier
Summary: The study showed that SINE1 and SINE2 play important roles in actin pattern changes during ABA-induced stomatal closure, but at different time steps. External Ca2+ can partially rescue the mutant effects. Nuclear Ca2+ oscillations during ABA-induced stomatal closure were observed for the first time, and were disrupted in the mutant. Vacuolar fragmentation was impaired in the mutants and could be partially rescued by F-actin depolymerization.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Ryota Sakamoto, Ziane Izri, Yuta Shimamoto, Makito Miyazaki, Yusuke T. Maeda
Summary: Cell migration in confined environments is crucial for various biological processes. This study investigates the physical determinants of actomyosin-based migration in confined environments using an in vitro migratory cell model. The results reveal the importance of the physical interaction between actomyosin networks and the cell membrane in generating a propulsive force for cell movement.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Lucija Mijanovic, Igor Weber
Summary: Dictyostelium amoebae adhere to extracellular material using similar mechanisms to metazoan cells, despite lacking integrins. The adhesion complexes in free-living amoeboid cells have evolved to enable less specific interactions with diverse materials encountered in their natural habitat.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kaustav Bera, Alexander Kiepas, Ines Godet, Yizeng Li, Pranav Mehta, Brent Ifemembi, Colin D. Paul, Anindya Sen, Selma A. Serra, Konstantin Stoletov, Jiaxiang Tao, Gabriel Shatkin, Se Jong Lee, Yuqi Zhang, Adrianna Boen, Panagiotis Mistriotis, Daniele M. Gilkes, John D. Lewis, Chen-Ming Fan, Andrew P. Feinberg, Miguel A. Valverde, Sean X. Sun, Konstantinos Konstantopoulos
Summary: Cells can respond to changes in extracellular fluid viscosity, and higher viscosity can increase cell motility and dissemination. This is achieved through the formation of a dense actin network and the activation of specific signaling pathways. Additionally, cancer cells exposed to high viscosity can acquire mechanical memory and enhance migration and metastasis through transcriptional control.
Article
Multidisciplinary Sciences
Runchen Zhao, Siqi Cui, Zhuoxu Ge, Yuqi Zhang, Kaustav Bera, Lily Zhu, Sean X. Sun, Konstantinos Konstantopoulos
Summary: Cells transitioning from amoeboid to mesenchymal phenotypes in response to increasing hydraulic resistance exhibit oscillatory changes in actin network formation and focal adhesion maturation. Blocking the mechanosensor TRPM7 eliminates the dependence of transition period on resistance, and mathematical modeling reveals the role of intracellular calcium oscillations in this process. Hydraulic resistance emerges as a critical physical factor influencing cell phenotype, with a proposed framework for linking fluorescence signal fluctuations to morphological changes.
Article
Immunology
Lyndsay Avery, Tanner F. Robertson, Christine F. Wu, Nathan H. Roy, Samuel D. Chauvin, Eric Perkey, Ashley Vanderbeck, Ivan Maillard, Janis K. Burkhardt
Summary: X-MAID is a primary immunodeficiency disease characterized by severe lymphopenia and recurrent infections. Using a mouse model, researchers found that the disease is caused by a mutation in the protein moesin. This model provides valuable insights into the complexity of immunodeficiency and the role of the actin cortex in lymphocyte function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Urology & Nephrology
Anne K. Muehlig, Johanna Steingroever, Hannah S. Heidelbach, Madelaine Wingerath, Wiebke Sachs, Irm Hermans-Borgmeyer, Catherine Meyer-Schwesinger, Hoon Young Choi, Beom Jin Lim, Christian Patry, Georg Friedrich Hoffmann, Nicole Endlich, Katharina Bracke, Mariella Weiss, Andreas H. Guse, Moritz Lasse, Markus M. Rinschen, Fabian Braun, Tobias B. Huber, Victor G. Puelles, Claus Peter Schmitt, Jun Oh
Summary: Activation of podocyte CaSR regulates key podocyte functions and reduces toxin-induced proteinuria and glomerular damage.
KIDNEY INTERNATIONAL
(2022)
Article
Materials Science, Biomaterials
Adrian A. Shimpi, Erik D. Williams, Lu Ling, Tigist Tamir, Forest M. White, Claudia Fischbach
Summary: This study investigates tumor cell migration on fibrotic ECM using a cell-derived matrix (CDM) model and phosphotyrosine phosphoproteomics. The results demonstrate that tumor cells cultured on CDMs migrate faster and in a more directional manner, which correlates with decreased cell spreading and increased cell elongation. The study also suggests that LYN may be differentially regulated and involved in regulating tumor cell migration on CDMs.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)