Article
Cell Biology
Meng-Ju Lin, Chia-Ming Lee, Wei-Lin Hsu, Bi-Chang Chen, Shyh-Jye Lee
Summary: Macrophages may physically alleviate nerve inhibition and promote regeneration and development in the zebrafish lateral line.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Neurosciences
Frederic A. A. Vallejo, Anthony Diaz, Emily L. L. Errante, Taylor Smartz, Aisha Khan, Risset Silvera, Adriana E. E. Brooks, Yee-Shuan Lee, Stephen Shelby Burks, Allan D. D. Levi
Summary: This study systematically evaluated the therapeutic use of Schwann cells (SC) in the repair of peripheral nerve injuries. The results showed that SCs have clear potential in improving morphological and functional outcomes in animal studies of long gap peripheral nerve injuries. However, the optimal nerve conduit or graft for delivering and retaining SCs for nerve regeneration has yet to be determined.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Emily L. Errante, Anthony Diaz, Taylor Smartz, Aisha Khan, Risset Silvera, Adriana E. Brooks, Yee-Shuan Lee, S. Shelby Burks, Allan D. Levi
Summary: Peripheral nerve injury (PNI) is common in trauma patients, and severe PNI can be challenging to treat. The use of cell therapies, particularly Schwann cells, has shown effectiveness in PNI treatment. Our laboratory has demonstrated the effectiveness of Schwann cells in the treatment of severe PNI when used with axon guidance channels, but optimal cellular placement techniques are crucial.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Andres Fuentes-Flores, Cristian Geronimo-Olvera, Karina Girardi, David Necunir-Ibarra, Sandip Kumar Patel, Joanna Bons, Megan C. Wright, Daniel Geschwind, Ahmet Hoke, Jose A. Gomez-Sanchez, Birgit Schilling, Daniela L. Rebolledo, Judith Campisi, Felipe A. Court
Summary: Reparative Schwann cells transition into a senescent phenotype in aging and chronic denervation, leading to inhibition of axonal regeneration. Elimination of senescent cells improves nerve regeneration and functional recovery.
EMBO MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Jose A. Gomez-Sanchez, Nikiben Patel, Fernanda Martirena, Shaline Fazal, Clara Mutschler, Hugo Cabedo
Summary: The peripheral nervous system (PNS) has superior regenerative capacity compared to the central nervous system (CNS). This regenerative ability is attributed to the reprogramming of Schwann cells (SC) into repair Schwann cells, which support axonal growth, myelin degradation, neurotrophic factor secretion, and axonal growth guidance. Epigenetic regulations, particularly histone acetylation and deacetylation, play a crucial role in SC reprogramming and nerve regeneration. This review focuses on Zn2+-dependent histone deacetylases (HDACs) and their importance in repair SC biology and remyelination after PNS injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Neurosciences
Kristjan R. Jessen, Rhona Mirsky
Summary: After nerve injury, both Schwann cells and neurons switch to pro-regenerative states. However, the poor functional repair cell phenotype is an important reason for the poor outcomes after nerve damage.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biology
Tian-Kun Hui, Xin-Sheng Lai, Xia Dong, Hongyang Jing, Ziyang Liu, Erkang Fei, Wen-Bing Chen, Shunqi Wang, Dongyan Ren, Suqi Zou, Hai-Tao Wu, Bing-Xing Pan
Summary: Schwann cells play a crucial role in peripheral nerve regeneration, while the conditional knockout of Lrp4 in SCs contributes to enhanced nerve regeneration by promoting SC proliferation and myelin debris clearance. These results suggest a novel role for Lrp4 in peripheral nerve recovery and provide a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Carina Hromada, Jaana Hartmann, Johannes Oesterreicher, Anton Stoiber, Anna Daerr, Barbara Schaedl, Eleni Priglinger, Andreas H. Teuschl-Woller, Wolfgang Holnthoner, Johannes Heinzel, David Hercher
Summary: This study reveals increased lymphangiogenesis after peripheral nerve injury and repair. The findings suggest that Schwann cells may have an inhibitory effect on lymphatic vessel formation.
Article
Biochemistry & Molecular Biology
Luca Scaccini, Roberta Mezzena, Alessia De Masi, Mariacristina Gagliardi, Giovanna Gambarotta, Marco Cecchini, Ilaria Tonazzini
Summary: The study presents a method using chitosan scaffolds to promote nerve regeneration, showing that asymmetric directional topographies can enhance cell proliferation and migration speed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Peng Yu, Guanhua Zhang, Bo Hou, Enpeng Song, Jiaming Wen, Yueyang Ba, Donglin Zhu, Gangwei Wang, Feng Qin
Summary: Complete myelination and formation of myelinated fibers are essential for functional nerve regeneration after peripheral nerve injury. Suboptimal myelin regeneration is common and can hinder ideal nerve regeneration. Therefore, closely monitoring and supporting myelin regeneration in patients with PNI is important for optimal outcomes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Lucas Vinicius de Oliveira Ferreira, Beatriz da Costa Kamura, Joao Pedro Marmol De Oliveira, Natielly Dias Chimenes, Marcio de Carvalho, Leandro Alves dos Santos, Luciane Alarcao Dias-Melicio, Renee Laufer Amorim, Rogerio Martins Amorim
Summary: This study describes for the first time the in vitro transdifferentiation potential of equine adipose tissue-derived (AT-MSCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) into Schwann-like cells (SLCs) using a practical method. The MSCs from both sources exhibited similar morphology to Schwann cells (SCs) and maintained cell viability and metabolic activity. These findings indicate that AT-MSCs and BM-MSCs have great transdifferentiation potential into SLCs using this method and represent a promising strategy for cell-based therapy for peripheral nerve regeneration in horses.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Sailing Chen, Qianqian Chen, Xiaojiao Zhang, Yinying Shen, Xinyu Shi, Xiu Dai, Sheng Yi
Summary: Peripheral nerves have limited ability to regenerate after injury. The growth factor amphiregulin (AREG) is upregulated in Schwann cells of injured sciatic nerves and stimulates their proliferation and migration. Schwann cell-secreted AREG promotes neurite outgrowth and axon elongation. Administering AREG to injured nerves facilitates Schwann cell proliferation, cord formation, and axon regrowth. Overall, our study identifies AREG as an important neurotrophic factor and offers a promising therapeutic approach for peripheral nerve injuries.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yan Kong, Di Wang, Qufu Wei, Yumin Yang
Summary: In this study, a porous NDM-CS scaffold with high antimicrobial activity and high biocompatibility was prepared. The scaffold not only promoted nerve regeneration but also prevented wound infection. The positive effect on Schwann cells indicated its potential in nerve injury repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Neurosciences
Qing Min, David B. Parkinson, Xin-Peng Dun
Summary: Schwann cells in the peripheral nervous system have the ability to promote repair of various tissues, including nerve gaps, skin wounds, digit tips, and tooth regeneration. They are highly motile, secrete signaling molecules, attract macrophages, support neuronal survival, promote axonal regrowth, and interact with other cell types in the regeneration process. The migration of Schwann cells is essential for successful tissue repair, especially in cases of peripheral nerve transection injuries where the cells form cords to guide axon regeneration. Understanding the molecular mechanisms of Schwann cell migration may lead to new therapeutic strategies for peripheral nerve repair.
Article
Nanoscience & Nanotechnology
Flavia Millesi, Sascha Mero, Lorenz Semmler, Anda Rad, Sarah Stadlmayr, Anton Borger, Paul Supper, Maximilian Haertinger, Leon Ploszczanski, Ursula Windberger, Tamara Weiss, Aida Naghilou, Christine Radtke
Summary: This study compared various commercially available hydrogels and examined their effects on the morphology, viability, proliferation, and migration of nerve cells. The rheological properties and topography of the hydrogels were also analyzed. The results showed significant differences in cell elongation and directed migration on the hydrogels, which were driven by laminin and a porous, fibrous, and strain-stiffening matrix structure. This study enhances our understanding of cell-matrix interactions and provides technical support for the future fabrication of tailored hydrogels.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Multidisciplinary Sciences
Myra N. Chavez, Rodrigo A. Morales, Camila Lopez-Crisosto, Juan Carlos Roa, Miguel L. Allende, Sergio Lavandero
SCIENTIFIC REPORTS
(2020)
Article
Zoology
Nicolas Cumplido, Miguel L. Allende, Gloria Arratia
FRONTIERS IN ZOOLOGY
(2020)
Article
Multidisciplinary Sciences
Laura Wisniewski, Vanessa French, Nicola Lockwood, Leonardo E. Valdivia, Paul Frankel
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Javiera F. de la Paz, Consuelo Anguita-Salinas, Cesar Diaz-Celis, Francisco P. Chavez, Miguel L. Allende
Biographical-Item
Developmental Biology
Fernando Casares, Miguel Allende, Jose Felix de Celis, Acaimo Gonzalez-Reyes, Juan Ramon Martinez-Morales
Review
Biochemistry & Molecular Biology
David Gonzalez, Miguel L. Allende
Summary: Wallerian degeneration is a genetically conserved mechanism where peripheral nervous system axons have a higher capacity to regenerate. The use of zebrafish larvae and time-lapse microscopy allows for monitoring of the regenerative process of peripheral nerves.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Susana Paredes-Zuniga, Fernando Ormeno, Miguel L. Allende
Summary: Triadimefon (TDF) is a fungicide used in agricultural crops, which affects the levels of dopamine and serotonin in the brain of vertebrates. This study shows that TDF induces both preference and aversion in zebrafish, depending on the dosage used. The lower dose leads to aversion while the higher dose leads to preference, indicating a complex relationship between TDF and behavioral responses in zebrafish.
NEUROTOXICOLOGY AND TERATOLOGY
(2021)
Review
Genetics & Heredity
Lara J. Monteiro, Reyna Penailillo, Mario Sanchez, Stephanie Acuna-Gallardo, Max Monckeberg, Judith Ong, Mahesh Choolani, Sebastian E. Illanes, Gino Nardocci
Summary: PE and IUGR are two prevalent pregnancy-specific placental disorders with high morbidity and mortality rates, and lncRNAs play a crucial role in regulating trophoblasts during the development of these diseases.
Article
Hematology
Oscar A. Pena, Alexandra Lubin, Catherine Hockings, Jasmine Rowell, Youngrock Jung, Yvette Hoade, Phoebe Dace, Leonardo E. Valdivia, Karin Tuschl, Charlotta Boiers, Maria C. Virgilio, Simon Richardson, Elspeth M. Payne
Summary: The researchers developed a zebrafish model of MDS and identified imiquimod as a compound that can alleviate anemia by promoting hematopoietic stem and progenitor cell expansion. They also found that TLR7 ligation might play a key role in anemia treatment.
Article
Biochemistry & Molecular Biology
David Munoz, Ricardo Fuentes, Beatriz Carnicero, Andrea Aguilar, Nataly Sanhueza, Sergio San-Martin, Cristian Agurto, Andrea Donoso, Leonardo E. Valdivia, Jesus M. Miguez, Lluis Tort, Sebastian Boltana
Summary: Feeding behavior in fish is influenced by multiple signals in the brain, with stress cues such as viral infection triggering responses from hypothalamic neurons to regulate energy storage and expenditure. This study provides evidence of a molecular linkage between inflammation and food intake in Salmon salar, highlighting the importance of understanding the interplay between energy homeostasis and pathogenesis in fish.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Anatomy & Morphology
Katherine J. Turner, Thomas A. Hawkins, Pedro M. Henriques, Leonardo E. Valdivia, Isaac H. Bianco, Stephen W. Wilson, Monica Folgueira
Summary: This study tracks and describes the development of different cell populations in the zebrafish telencephalon during the process of eversion using transgenic fish and structural markers, forming an atlas of telencephalic development. Additionally, they compare the transgenic expression with existing anatomical data.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Biochemistry & Molecular Biology
Reyna Penailillo, Lara J. Monteiro, Stephanie Acuna-Gallardo, Felipe Garcia, Victoria Velasquez, Paula Correa, Pilar Diaz, Patricia P. Valdebenito, Cristina Navarro, Roberto Romero, Mario Sanchez, Sebastian E. Illanes, Gino Nardocci
Summary: This study aimed to identify potential lncRNA biomarkers for preeclampsia by analyzing the expression of lncRNAs in the placentas and plasma of patients. Six lncRNAs were validated and differentially expressed in preeclampsia placentas, two of which were also detected in maternal plasma, indicating their potential use as biomarkers for the disease.
Article
Biochemistry & Molecular Biology
Sandra Arancibia-Opazo, J. Sebastian Contreras-Riquelme, Mario Sanchez, Marisol Cisternas-Olmedo, Rene L. Vidal, Alberto J. M. Martin, Mauricio A. Saez
Summary: Huntington's disease (HD) is caused by an abnormal expansion of trinucleotide CAG repeats within the huntingtin (Htt) gene, resulting in altered histone acetylation patterns and transcriptional deregulation. In this study, we analyzed differential gene expression and H3K27ac variation in R6/2 mice, a model of juvenile HD, and identified key regulators involved in the altered transcription cascade. Our findings suggest changes in acetylation and gene expression levels related to impaired neuronal development, and propose key regulators that drive the downstream regulatory cascade in HD. This study provides the first approach to understanding epigenetic changes in the early stages of HD and highlights the importance of identifying early onset indicators of disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Developmental Biology
Rebecca Wycliffe, Julie Plaisancie, Sydney Leaman, Octavia Santis, Lisa Tucker, Daniela Cavieres, Michelle Fernandez, Camila Weiss-Garrido, Cristian Sobarzo, Gaia Gestri, Leonardo E. Valdivia
Summary: The mab21l2(u517) mutant exhibits complex eye morphogenesis phenotype, characterized by delayed growth, excess progenitors, and asynchronous neurogenesis, suggesting a role of mab21l2 in coordinating optic cup development, timely neurogenesis, and progenitor allocation in zebrafish ciliary marginal zone.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Giuseppina Covello, Fernando J. Rossello, Michele Filosi, Felipe Gajardo, Anne-Laure Duchemin, Beatrice F. Tremonti, Michael Eichenlaub, Jose M. Polo, David Powell, John Ngai, Miguel L. Allende, Enrico Domenici, Mirana Ramialison, Lucia Poggi