Article
Biology
Shuo Wang, Lukas Findeisen, Sebastian Leptihn, Mark I. Wallace, Marcel Hoerning, Stephan Nussberger
Summary: Wang et al. used single-molecule measurements to uncover a potentially new mechanosensitive functionality of mitochondrial TOM core complexes. They found that TOM-CC switches reversibly between different ion permeability states associated with protein diffusion. This behavior is explained by the mechanical binding of TOM-CC to a hydrogel and the opening and closing of beta-barrel pores.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiyao Song, Liesa Steidle, Isabelle Steymans, Jasjot Singh, Anne Sanner, Lena Boettinger, Dominic Winter, Thomas Becker
Summary: Mitochondrial Hsp70 plays dual roles in the formation of ATP synthase. It cooperates with Atp11 and Atp12 to form the F-1 domain, and it transfers Atp5 to link the catalytic head with the peripheral stalk. Inactivation of mtHsp70 leads to integration of assembly-defective Atp5 variants into the mature complex, indicating its quality control function.
NATURE COMMUNICATIONS
(2023)
Review
Physiology
Bogusz Kulawiak, Adam Szewczyk
Summary: This paper critically reviews the current challenges in mitochondrial potassium channels research. Although there has been substantial progress in understanding K+ traffic in mitochondria, some fundamental issues in this process remain unresolved. The molecular identity of various mitochondrial potassium channels and their interactions with other mitochondrial proteins are discussed in this paper. The posttranslational modifications and pharmacological targeting of mitochondrial potassium channels are also mentioned.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Samuel Rout, Silke Oeljeklaus, Abhijith Makki, Jan Tachezy, Bettina Warscheid, Andre Schneider
Summary: The study reveals that the mitochondrial import receptors ATOM46 and ATOM69 of Trypanosoma brucei have different substrate preferences, with ATOM46 favoring hydrophilic proteins and ATOM69 preferring hydrophobic substrates. While ATOM46 mainly uses electrostatic interactions for substrate binding, ATOM69 primarily uses hydrophobic interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Qiang Wang, Zeyuan Guan, Liangbo Qi, Jinjin Zhuang, Chen Wang, Sixing Hong, Ling Yan, Yan Wu, Xiaoqian Cao, Jianbo Cao, Junjie Yan, Tingting Zou, Zhu Liu, Delin Zhang, Chuangye Yan, Ping Yin
Summary: Sam37 stabilizes mature Tom40 mainly through electrostatic interactions, facilitating subsequent TOM assembly. These results support the b barrel switching model and offer structural insights into the assembly and release of b barrel complexes.
Article
Biochemistry & Molecular Biology
Naintara Jain, Ridhima Gomkale, Olaf Bernhard, Peter Rehling, Luis Daniel Cruz-Zaragoza
Summary: Mitochondria play a crucial role in cellular energy production and metabolism, and errors in mitochondrial protein import can lead to metabolic disorders. This study establishes a fluorescence-based import assay to analyze protein import into mitochondria, offering the advantage of quantifying import with high sensitivity. The assay can be adapted to a screening-compatible format and used to monitor the assembly of the F1F0 ATP synthase in purified mitochondria.
Article
Biology
Liam P. Coyne, Xiaowen Wang, Jiyao Song, Ebbing de Jong, Karin Schneider, Paul T. Massa, Frank A. Middleton, Thomas Becker, Xin Jie Chen
Summary: This study reveals that amino acid substitutions in a single substrate preprotein can disrupt protein traffic into mitochondria, impair mitochondrial function and cell viability. The mutant Aac2/ANT1 shows extreme toxicity in yeast and leads to muscle disease in mice. These findings highlight the importance of avoiding clogging in mitochondrial carrier proteins for preventing disease.
Review
Biochemistry & Molecular Biology
Ruairidh Edwards, Ross Eaglesfield, Kostas Tokatlidis
Summary: The mitochondrial intermembrane space (IMS) is a narrow sub-mitochondrial compartment with diverse protein import mechanisms. IMS proteins do not require energy to cross the outer mitochondrial membrane, unlike import into the matrix or inner membrane. Many IMS proteins are associated with human diseases, highlighting the importance of understanding their normal function and localization in the IMS.
Article
Multidisciplinary Sciences
Laura F. Fielden, Jakob D. Busch, Sandra G. Merkt, Iniyan Ganesan, Conny Steiert, Hanna B. Hasselblatt, Jon V. Busto, Christophe Wirth, Nicole Zufall, Sibylle Jungbluth, Katja Noll, Julia M. Dung, Ludmila Butenko, Karina von der Malsburg, Hans-Georg Koch, Carola Hunte, Martin van der Laan, Nils Wiedemann
Summary: The presequence translocase of the mitochondrial inner membrane (TIM23) is the major route for importing nuclear-encoded proteins into mitochondria. Tim17, a subunit of TIM23, interacts with preproteins in the matrix or inner membrane for protein translocation. The negative charges in Tim17 initiate the translocation mechanism and release the preproteins into the inner membrane.
Article
Biochemistry & Molecular Biology
Emily Wachoski-Dark, Tian Zhao, Aneal Khan, Timothy E. Shutt, Steven C. Greenway
Summary: There is a unclear mechanistic link between human mitochondrial disorders and the development of cardiomyopathy. Defects in mitochondrial proteostasis, which involves the maintenance of mitochondrial protein homeostasis, can lead to mitochondrial cardiomyopathies. Disruptions in proteostasis can result in mitochondrial dysfunction and impact cardiac function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Endocrinology & Metabolism
Mohamed A. Eldeeb, Andrea Soumbasis, Edward A. Fon
Summary: Given their polyvalent roles, mitochondria face the challenge of continuous exposure to stressors, such as mitochondrial import defects, which result in dysfunction. Recent research has discovered a quality control pathway, dependent on a presequence translocase-associated import motor (PAM) complex, in which misfolded proteins reduce mitochondrial protein import and subsequently trigger mitophagy without loss of mitochondrial membrane potential.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2023)
Review
Genetics & Heredity
Eduardo Ruiz-Pesini, Julio Montoya, David Pacheu-Grau
Summary: In human mitochondria, mtDNA encodes a small fraction of proteins while the majority are encoded by the nuclear genome and imported into mitochondria through various import machineries. Proper coordination of these molecular pathways is crucial for mitochondrial homeostasis. Studying these pathways is important for understanding and expanding the genetic landscape of mitochondrial diseases.
Article
Biochemistry & Molecular Biology
Chang-Yong Choi, Mai Tram Vo, John Nicholas, Young Bong Choi
Summary: Our study reveals that the mitochondrial translation elongation factor Tu located on the outer membrane of mitochondria can inhibit altered mitochondria-induced apoptosis through its autophagic function. The autophagy-competent TUFM is required for self-dimerization and mitophagy, and its stabilization upon autophagy activation could inhibit caspase-8 activation, providing insights into the regulation of apoptosis by mitophagy.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Max-Hinderk Schuler, Alyssa M. English, Tianyao Xiao, Thane J. Campbell, Janet M. Shaw, Adam L. Hughes
Summary: Amino acids are crucial for cellular function, but elevated levels can lead to cellular toxicity. The mitochondrial-derived compartment (MDC) has been identified as a protective mechanism against amino acid stress, promoting amino acid catabolism and maintaining homeostasis through nutrient transporter remodeling.
Review
Biochemistry & Molecular Biology
Juliane J. Hoffmann, Thomas Becker
Summary: Mitochondria import precursor proteins from cytosol through translocase complexes. The phospholipid composition of mitochondrial membranes affects the stability and activity of protein translocases, as well as the rearrangement and distortion of lipid bilayer during protein integration. Phospholipids also play a critical role in respiratory chain activity and membrane potential generation for protein import. The close link between outer membrane protein translocases and lipid trafficking at contact sites further controls mitochondrial biogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Alexander Benjamin Schendzielorz, Christian Schulz, Oleksandr Lytovchenko, Anne Clancy, Bernard Guiard, Raffaele Ieva, Martin van der Laan, Peter Rehling
JOURNAL OF CELL BIOLOGY
(2017)
Article
Cell Biology
Ajay Ramesh, Valentina Peleh, Sonia Martinez-Caballero, Florian Wollweber, Frederik Sommer, Martin van der Laan, Michael Schroda, R. Todd Alexander, Maria Luisa Campo, Johannes M. Herrman
JOURNAL OF CELL BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Ralf M. Zerbes, Philipp Hoss, Nikolaus Pfanner, Martin van der Laan, Maria Bohnert
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Uma Turakhiya, Karina von der Malsburg, Vicki A. M. Gold, Bernard Guiard, Agnieszka Chacinska, Martin van der Laan, Raffaele Ieva
JOURNAL OF MOLECULAR BIOLOGY
(2016)
Article
Cell Biology
Zeynep Cakir, Kathrin Funk, Joachim Lauterwasser, Franziska Todt, Ralf M. Zerbes, Aline Oelgeklaus, Atsushi Tanaka, Martin van der Laan, Frank Edlich
JOURNAL OF CELL SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Heike Rampelt, Maria Bohnert, Ralf M. Zerbes, Susanne E. Horvath, Bettina Warscheid, Nikolaus Pfanner, Martin van der Laan
JOURNAL OF MOLECULAR BIOLOGY
(2017)
Article
Multidisciplinary Sciences
Manuel Hessenberger, Ralf M. Zerbes, Heike Rampelt, Severine Kunz, Audrey H. Xavier, Bettina Purfuerst, Hauke Lilie, Nikolaus Pfanner, Martin van der Laan, Oliver Daumke
NATURE COMMUNICATIONS
(2017)
Article
Cell Biology
Marcel Morgenstern, Sebastian B. Stiller, Philipp Luebbert, Christian D. Peikert, Stefan Dannenmaier, Friedel Drepper, Uri Weill, Philipp Hoess, Reinhild Feuerstein, Michael Gebert, Maria Bohnert, Martin van der Laan, Maya Schuldiner, Conny Schuetze, Silke Oeljeklaus, Nikolaus Pfanner, Nils Wiedemann, Bettina Warscheid
Article
Cell Biology
Ayelen Gonzalez Montoro, Kathrin Auffarth, Carina Hoenscher, Maria Bohnert, Thomas Becker, Bettina Warscheid, Fulvio Reggiori, Martin van der Laan, Florian Froehlich, Christian Ungermann
DEVELOPMENTAL CELL
(2018)
Article
Biochemistry & Molecular Biology
Heike Rampelt, Florian Wollweber, Carolin Gerke, Rinse de Boer, Ida J. van der Klei, Maria Bohnert, Nikolaus Pfanner, Martin van der Laan
JOURNAL OF MOLECULAR BIOLOGY
(2018)
Review
Cell Biology
Stefan Schorr, Martin van der Laan
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Irina Kurokin, Anna Andrea Lauer, Daniel Janitschke, Jakob Winkler, Elena Leoni Theiss, Lea Victoria Griebsch, Sabrina Melanie Pilz, Veronika Matschke, Martin van der Laan, Heike Sabine Grimm, Tobias Hartmann, Marcus Otto Walter Grimm
Summary: The study investigated potential alterations in the lipid composition of mitochondria in a cellular AD model, revealing specific lipid changes in mitochondria that cannot be predicted from total cell analysis. The observed lipid alterations are accompanied by changes in the carnitine carrier system, suggesting altered mitochondrial functionality, which may be related to differences in APP processing.
Article
Biochemistry & Molecular Biology
Florian Wollweber, Karina von der Malsburg, Martin van der Laan
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2017)
Article
Oncology
Veronica I. Dumit, Ralf M. Zerbes, Stephanie Kaeser-Pebernard, Michal Rackiewicz, Mona T. Wall, Christine Gretzmeier, Victoria Kuettner, Martin van der Laan, Ralf J. Braun, Joern Dengjel
Review
Biochemistry & Molecular Biology
Heike Rampelt, Ralf M. Zerbes, Martin van der Laan, Nikolaus Pfanner
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2017)
