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Multidisciplinary Sciences
Monika Gonczi, Joao M. C. Teixeira, Susana Barrera-Vilarmau, Laura Mediani, Francesco Antoniani, Tamas Milan Nagy, Krisztina Feher, Zsolt Raduly, Viktor Ambrus, Jozsef Tozser, Endre Barta, Katalin E. Kover, Laszlo Csernoch, Serena Carra, Monika Fuxreiter
Summary: The context-dependent higher-order assembly of Mef2D controls muscle lineage development by simultaneously interacting with the basal machinery and myogenic factors. The study found that the alternatively spliced acidic beta-domain enhances the transcription of Mef2D. The beta-domain can serve as an interaction element for higher-order assembly, forming nuclear condensates and solid-like aggregates. These findings suggest that the beta-domain fine-tunes Mef2D assembly and provides a platform for myogenic regulatory factors and transcriptional apparatus during muscle development.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Charlotte Rivas, Jessica A. Jackson, Alex Rigby, James A. Jarvis, Andrew J. P. White, Philip J. Blower, Andreas Phanopoulos, Michelle T. . Ma
Summary: Hydroxypyridinone-based chelators have been found to be useful in incorporating radioactive metal ions into nuclear medicine diagnostic and therapeutic agents. In this study, two novel chelators with hydroxypyridinone groups were prepared and their reactivity towards radiometal-mediated hydrolysis was investigated. The results showed that the L1 complex exhibited a higher reactivity towards hydrolysis compared to the L2 complex. These findings were further supported by density functional theory calculations. This study highlights the importance of proper characterization of radiolabeled products.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Martino Colombo, Evangelos D. Karousis, Joel Bourquin, Remy Bruggmann, Oliver Muhlemann
Review
Virology
Giuseppe Balistreri, Claudia Bognanni, Oliver Mhlemann
Article
Biochemistry & Molecular Biology
Pamela Nicholson, Asimina Gkratsou, Christoph Josi, Martino Colombo, Oliver Muhlemann
Review
Cell Biology
Sofia Nasif, Lara Contu, Oliver Muhlemann
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2018)
Article
Cell Biology
Evangelos D. Karousis, Oliver Muhlemann
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2019)
Correction
Biochemistry & Molecular Biology
Katharina Schubert, Evangelos D. Karousis, Ahmad Jomaa, Alain Scaiola, Blanca Echeverria, Lukas-Adrian Gurzeler, Marc Leibundgut, Volker Thiel, Oliver Muhlemann, Nenad Ban
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Katharina Schubert, Evangelos D. Karousis, Ahmad Jomaa, Alain Scaiola, Blanca Echeverria, Lukas-Adrian Gurzeler, Marc Leibundgut, Volker Thiel, Oliver Muehlemann, Nenad Ban
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Giuditta Annibaldis, Michal Domanski, Rene Dreos, Lara Contu, Sarah Carl, Nina Klaey, Oliver Muehlemann
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Evangelos D. Karousis, Lukas-Adrian Gurzeler, Giuditta Annibaldis, Rene Dreos, Oliver Muehlemann
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Stefan Reber, Daniel Jutzi, Helen Lindsay, Anny Devoy, Jonas Mechtersheimer, Brunno Rocha Levone, Michal Domanski, Eva Bentmann, Dorothee Dormann, Oliver Muehlemann, Silvia M. L. Barabino, Marc-David Ruepp
Summary: Liquid-liquid phase separation (LLPS) of proteins and RNAs is critical in the formation of membrane-less organelles, leading to biomolecular condensates with diverse biological functions and disease implications. The Fused in Sarcoma (FUS) protein undergoes LLPS and its mutations are linked to Amyotrophic Lateral Sclerosis (ALS-FUS). The study shows that LLPS shapes the interactome of FUS in cells, affecting its function and disease outcomes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yeonkyoung Park, Joori Park, Hyun Jung Hwang, Leehyeon Kim, Kwon Jeong, Hyun Kyu Song, Simone C. Rufener, Oliver Muehlemann, Yoon Ki Kim
Summary: It has been demonstrated that CTIF is tethered to the perinuclear region by DDX19B in a translationally incompetent manner, and then handed over to CBP80, which is associated with the 5'-cap of newly exported mRNA, initiating CBC-dependent translation in the perinuclear region. Disrupting the interaction between CTIF and DDX19B leads to uncontrolled translation in the cytosol, ultimately affecting nonsense-mediated mRNA decay. This highlights the importance of tight control of local translation in the perinuclear region.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Lara Contu, Giuseppe Balistreri, Michal Domanski, Anne-Christine Uldry, Oliver Muhlemann
Summary: Understanding the interactions between Semliki Forest Virus proteins and host cell proteins is crucial for understanding the mechanisms by which the virus replicates successfully and develops specific antiviral interventions. The study shows that the virus hijacks the translation machinery and inhibits NMD to escape the host cell's defense mechanisms, with the capsid protein playing a key role in inhibiting NMD during the infectious cycle.
Article
Biochemistry & Molecular Biology
Lukas-Adrian Gurzeler, Jana Ziegelmueller, Oliver Muehlemann, Evangelos D. Karousis
Summary: This study introduces a robust and fast method for detergent-free cell lysis under controlled mechanical forces, which can produce translation-competent lysates from human cells and enhance protein output by reducing the phosphorylation state of eIF2 alpha.
Article
Cell Biology
Lukas-Adrian Gurzeler, Marion Link, Yvonne Ibig, Isabel Schmidt, Olaf Galuba, Julian Schoenbett, Christelle Gasser-Didierlaurant, Christian N. Parker, Xiaohong Mao, Francis Bitsch, Markus Schirle, Philipp Couttet, Frederic Sigoillot, Jana Ziegelmueller, Anne-Christine Uldry, Wojciech Teodorowicz, Niko Schmiedeberg, Oliver Muhlemann, Jurgen Reinhardt
Summary: This study discovered two potent readthrough promoters that can restore full-length functional proteins in disease models. Unlike other readthrough promoters, these compounds stimulate premature termination codon suppression by promoting the degradation of the translation termination factor eRF1.
Article
Biotechnology & Applied Microbiology
Evangelos D. Karousis, Foivos Gypas, Mihaela Zavolan, Oliver Muehlemann
Summary: The study utilized long-read sequencing and short-read sequencing to identify and analyze endogenous targets of nonsense-mediated mRNA decay (NMD) in human cells. It discovered many novel NMD-sensitive mRNA isoforms, most of which derive from alternative exon usage, and revealed the role of NMD in regulating gene expression through splicing signals analysis.
