Article
Engineering, Biomedical
Katily Ramirez, Shuya Kyu, Doan Nguyen, So-Yun Han, Ye Lim Lee, John Bradley, Troy Randall, Ignacio Sanz, Frances Eun-Hyung Lee, Todd Sulchek
Summary: The study demonstrates a high throughput single-cell protein secretion analysis and isolation method to isolate antibody-secreting cells specific for influenza virus protein from B cells. Using heterofunctional particles, fluorescent labeled antigens, and FACS, specific cells can be enriched effectively, improving antibody collection efficiency. Targeted cells can be identified with high sensitivity and specificity through this workflow.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Sangwook Oh, Xuming Mao, Silvio Manfredo-Vieira, Jinmin Lee, Darshil Patel, Eun Jung Choi, Andrea Alvarado, Ebony Cottman-Thomas, Damian Maseda, Patricia Y. Y. Tsao, Christoph T. T. Ellebrecht, Sami L. L. Khella, David P. P. Richman, Kevin C. C. O'Connor, Uri Herzberg, Gwendolyn K. K. Binder, Michael C. C. Milone, Samik Basu, Aimee S. S. Payne
Summary: Engineered T cells expressing MuSK-CAART effectively target B cells expressing anti-MuSK autoantibodies, reducing muscle weakness caused by autoimmune disease.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Natalia Sevillano, Evan M. Green, Jorg Votteler, Dong Young Kim, Xuefeng Ren, Bei Yang, Xi Liu, Andre Luiz Lourenco, James H. Hurley, Shauna Farr-Jones, John D. Gross, Yifan Cheng, Charles S. Craik
Summary: This study identified human, recombinant antibodies from a phage display library that bind to three HIV-host complexes, which were further converted into scFvs for structural and functional studies, providing essential antibody reagents for research and clinical applications. The rapid screening pipeline developed in this study can be applied to other challenging antigens, facilitating the generation of specific, reproducible, and renewable antibody reagents.
Article
Immunology
Jesse Bodle, Kirsten Vandenberg, Karen Laurie, Ian G. Barr, Ying Zhang, Steven Rockman
Summary: This study aimed to determine the applicability of enzyme linked immunoassay (ELISA) in quantifying and assessing the stability of different influenza vaccines. The ELISA, utilizing stable-indicating monoclonal antibodies (mAbs), accurately measures the HA antigen content and stability, with higher sensitivity and specificity towards different influenza B virus strains. Moreover, the ELISA can accurately quantify HA from different platforms and processing stages. Therefore, it is a suitable replacement for the traditional single radial immunodiffusion (SRID) assay.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Julia Bitencourt, Marco Polo Peralta-Alvarez, Morven Wilkie, Ashley Jacobs, Daniel Wright, Salem Salman Almujri, Shuailin Li, Stephanie A. Harris, Steven G. Smith, Sean C. Elias, Andrew D. White, Iman Satti, Sally S. Sharpe, Matthew K. O'Shea, Helen McShane, Rachel Tanner
Summary: Tuberculosis is a major health problem worldwide, and the currently-licensed BCG vaccine is not effective enough. Research has shown that the BCG vaccine can induce specific antibodies, which play a crucial role in cellular defense. These findings suggest that the humoral immune response should be further investigated in TB vaccine development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Wei Sun, Priyanka Khare, Xiaoli Wang, Dilip K. Challa, Benjamin M. Greenberg, Raimund J. Ober, E. Sally Ward
Summary: A new antibody-based therapeutic approach has been developed to selectively eliminate MOG-specific antibodies in autoimmune diseases, resulting in disease amelioration. Treatment delivers targeted antibodies to Kupffer and liver sinusoidal endothelial cells, showing therapeutic effects. This approach has the potential to treat other autoimmune diseases requiring specific antibody clearance.
Article
Multidisciplinary Sciences
Mohammad Ahmad, Jeung-Hoi Ha, Lauren A. Mayse, Maria F. Presti, Aaron J. Wolfe, Kelsey J. Moody, Stewart N. Loh, Liviu Movileanu
Summary: Protein detection is important in molecular diagnostics. Nanopores and the resistive-pulse technique have been used to analyze proteins. However, there is still a challenge in implementing specific interfaces for detecting proteins without the restrictions of the nanopore interior.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Yong Zhang, Wanjun Zhao, Yonghong Mao, Yaohui Chen, Shisheng Wang, Yi Zhong, Tao Su, Meng Gong, Dan Du, Xiaofeng Lu, Jingqiu Cheng, Hao Yang
Summary: This study characterized the N-glycosylation of the S protein of SARS-CoV-2, revealing differences in glycan type and abundance between human and insect cell-expressed proteins. These findings shed light on the infection mechanism and offer new perspectives for the development of vaccines and targeted drugs.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemical Research Methods
Ryan Kilgore, Wenning Chu, Dipendra Bhandari, David Fischler, Ruben G. Carbonell, Michael Crapanzano, Stefano Menegatti
Summary: We discovered peptide ligands that target different regions of human Fab and enable product release under mild conditions, with excellent binding capacity and dissociation constant. The selected peptide ligand showed comparable yield and purity to commercial Protein L resins in the purification of polyclonal and monoclonal Fabs.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Review
Biochemistry & Molecular Biology
Maria Gabriella Dona, Paola Di Bonito, Maria Vincenza Chiantore, Carla Amici, Luisa Accardi
Summary: In recent decades, recombinant antibodies targeting HPV oncoproteins have shown great promise for the therapy of HPV-associated cancers. However, the critical issue of delivering these antibodies to tumor cells must be addressed to achieve successful translation from the laboratory to the clinic.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Zamrina Baharudeen, Rahmah Noordin, Lim Theam Soon, Dinesh Balachandra, Nor Suhada Anuar, Fatin Hamimi Mustafa, Anizah Rahumatullah
Summary: This study successfully isolated and produced novel recombinant monoclonal antibodies against Toxocara canis recombinant TES-26 antigen. The antibodies showed high specificity and sensitivity, and demonstrated potential diagnostic and therapeutic applications.
Article
Multidisciplinary Sciences
Anish Yadav, Shafiya Imtiaz Rafiqi, Vikas Yadav, Anand Kushwaha, Rajesh Godara, Shilpa Sood, Mohd Altaf Bhat, Rajesh Katoch, Rosario Panadero-Fontan
Summary: This study presents an optimised indirect-ELISA based on recombinant HyC derived from P. silenus for early diagnosis of GWFI, with diagnostic specificity and sensitivity of 96% and 100%, respectively, and no cross reactivity against other important parasitic and bacterial diseases of goats.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, General & Internal
Michal Gniewkiewicz, Katarzyna Czerwinska, Katarzyna Zielniok, Magdalena Durlik
Summary: This study investigated the association between circulating DSAs and their characteristics with renal allograft long-term outcomes. The detection of DSAs at the time of biopsy and their C1q-binding capacity were found to be independent predictors of inferior renal allograft function and graft failure in kidney transplant recipients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mikail D. Levasseur, Shiksha Mantri, Takahiro Hayashi, Maria Reichenbach, Svenja Hehn, Ying Waeckerle-Men, Pal Johansen, Donald Hilvert
Summary: This study explores a new application method for protein nanocages, enabling precise binding of targeting immunoglobulins and intracellular uptake of related cargos. However, the potential drawback of this technology is the masking effect of serum antibodies, which may diminish the efficacy of the targeting antibodies.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Tao Dong, Xin Zhang, Jinlong Yuan, Ziting Lin, Pengxue Yin, Haipeng Yu, Mingyang Wang, Aihua Liu
Summary: A peptide-based lateral flow immunoassay (pLFIA) for sensitive detection of the SARS-CoV-2 spike antigen was developed using a specific peptide identified by a nonamplified biopanning method. The pLFIA showed excellent analytical performance and could detect the SARS-CoV-2 antigen at a low limit of detection, making it a reliable option for rapid diagnosis of SARS-CoV-2.
ANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Sabrina Karim-Silva, Alessandra Becker-Finco, Isabella Gizzi Jiacomini, Fanny Boursin, Arnaud Leroy, Magali Noiray, Juliana de Moura, Nicolas Aubrey, Philippe Billiald, Larissa M. Alvarenga
Article
Pharmacology & Pharmacy
Lionel Renaud, Kristell Lebozec, Christine Voors-Pette, Peter Dogterom, Philippe Billiald, Martine Jandrot Perrus, Yannick Pletan, Matthias Machacek
JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Article
Multidisciplinary Sciences
Dhruv Gandhi, Peng Li, Stefano Rampini, Charlotte Parent, Gil U. Lee
SCIENTIFIC REPORTS
(2020)
Article
Parasitology
Michele Martha Weber-Lima, Bianca Prado-Costa, Alessandra Becker-Finco, Adriana Oliveira Costa, Philippe Billilad, Cinthia Furst, Juliana Ferreira de Moura, Larissa Magalhaes Alvarenga
Article
Biochemical Research Methods
Gavin McGauran, Emma Dorris, Razvan Borza, Niamh Morgan, Denis C. Shields, David Matallanas, Anthony G. Wilson, David J. O'Connell
Article
Medicine, General & Internal
Thomas Reiter, Sahra Pajenda, David O'Connell, Ciara Lynch, Sebastian Kapps, Hermine Agis, Alice Schmidt, Ludwig Wagner, Nelson Leung, Wolfgang Winnicki
Summary: Overproduction of human light chains and immunoglobulins can lead to various renal diseases through complex interactions. Recent protein analysis methods have enabled the identification of interacting proteins and their clinical relevance. Further biochemical studies are needed to elucidate the impact of these interactions on disease pathology.
FRONTIERS IN MEDICINE
(2021)
Article
Multidisciplinary Sciences
Stefano Rampini, Peng Li, Dhruv Gandhi, Marina Mutas, Ying Fen Ran, Michael Carr, Gil U. Lee
Summary: Magnetically actuated lab-on-a-chip technologies enable rapid separation of specific biomarkers and cells, while nonlinear magnetophoresis allows precise control of superparamagnetic bead transport on chip surfaces. Optimization of micromagnet shape is crucial for improving the efficiency of magnetically actuated separation techniques.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Sahra Pajenda, Florence Zawedde, Sebastian Kapps, Ludwig Wagner, Alice Schmidt, Wolfgang Winnicki, David O'Connell, Daniela Gerges
Summary: For patients with acute kidney injury, urinary C3a/C3 shows an inverse correlation with serum CRP, serving as a potential inverse acute-phase parameter originating from the kidney and detectable in urine.
Article
Multidisciplinary Sciences
Ciara D. Lynch, David J. O'Connell
Summary: This study investigates the potential of using chemically defined spent media waste from cell culture bioprocess as a feed for secondary microbial fermentation to produce new recombinant protein products. The results show that the waste, when supplemented with glycerol, can support the growth rate of E. coli and achieve similar protein production as that of a nutritionally rich microbiological media. The quantitative analysis reveals changes in protein synthesis and carbohydrate metabolism enzymes in the waste-fed cultures.
Article
Biochemistry & Molecular Biology
Isabella Gizzi Jiacomini, Martina Beltramino, Fanny Boursin, Joao Carlos Minozzo, Juliana Ferreira de Moura, Philippe Billiald, Larissa Magalhaes Alvarenga, Nicolas Aubrey
Summary: The use of monoclonal antibodies in therapy is expanding, with focus on safety, cost-effectiveness, and efficacy. Recombinant DNA technology is used for large-scale production, and strategies for humanization have been developed considering their heterologous origin and potential immunogenicity. This study introduced a mouse monoclonal antibody with binding and neutralizing abilities against spider venom toxins, and explored its humanization potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Hematology
Philippe Billiald, Alexandre Slater, Martin Welin, Joanne C. Clark, Stephane Loyau, Martine Pugniere, Isabella G. Jiacomini, Nadia Rose, Kristell Lebozec, Elie Toledano, Deborah Francois, Steve P. Watson, Martine Jandrot-Perrus
Summary: This study shows that glenzocimab, a humanized antibody fragment, blocks the binding of GPVI to vascular collagen and fibrin through steric hindrance and structural change. This finding is significant for the development of new antiplatelet molecules with a low bleeding risk.
Article
Chemistry, Multidisciplinary
Nathan Feely, Anita Wdowicz, Anne Chevalier, Ying Wang, Peng Li, Fanny Rollo, Gil U. U. Lee
Summary: The development of specific and sensitive immunomagnetic cell separation nanotechnologies is crucial for improving cancer patient outcomes. The use of limited biomarkers for enriching circulating tumor cells (CTCs) has resulted in low CTC yields and purity. This study demonstrates that cell bound MUC16 can be effectively targeted for rapid immunomagnetic extraction of CTCs with high yield, purity, and viability. The results also provide insights into the impact of nanoparticle physical properties on the efficiency of immunomagnetic cell isolation.
Article
Chemistry, Multidisciplinary
Cian Holohan, Nathan Feely, Peng Li, Gerard Curran, Gil U. Lee
Summary: The potential of liquid biopsy samples as a less invasive alternative to tissue biopsies is increasingly recognized. This study optimized the lysis reaction for extracting RNA from human melanoma cells using silica coated superparamagnetic nanoparticles. Surprisingly, the addition of sustainable surfactants did not significantly improve cell lysis or qRT-PCR sensitivity. Higher concentrations of serum albumin in the sample matrix decreased qRT-PCR sensitivity due to increased RNase activity.
Article
Chemistry, Multidisciplinary
Cian Holohan, Sophia Hanrahan, Nathan Feely, Peng Li, John O'Connell, Catherine Moss, Michael Carr, Oya Tagit, Gil U. Lee
Summary: The study found that the choice of viral transport media and freeze-thaw cycles significantly impact the sensitivity of COVID-19 molecular diagnostics assays, affecting the stability of the SARS-CoV-2 viral genome. Testing with glycerol-containing PBS is recommended for high stability and sensitivity, as supported by clinical studies on patient-derived samples.
Article
Chemistry, Multidisciplinary
Peng Li, Dhruv Gandhi, Marina Mutas, Yin-Fen Ran, Michael Carr, Stefano Rampini, William Hall, Gil U. Lee