Single-molecule methods leap ahead

A Viral Packaging Motor Varies Its DNA Rotation and Step Size to Preserve Subunit Coordination as the Capsid Fills

(2014) Shixin Liu et al.   CELL

Single-molecule motions enable direct visualization of biomolecular interactions in solution

(2014) Quan Wang et al.   NATURE METHODS

Transcription Under Torsion

(2013) J. Ma et al.   SCIENCE

High-Speed Force Spectroscopy Unfolds Titin at the Velocity of Molecular Dynamics Simulations

(2013) F. Rico et al.   SCIENCE

Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase

(2012) Elizabeth A Manrao et al.   NATURE BIOTECHNOLOGY

A toolkit and benchmark study for FRET-restrained high-precision structural modeling

(2012) Stanislav Kalinin et al.   NATURE METHODS

Single-Molecule Fluorescence Experiments Determine Protein Folding Transition Path Times

(2012) H. S. Chung et al.   SCIENCE

Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas

(2012) G. P. Acuna et al.   SCIENCE

Single-molecule approach to immunoprecipitated protein complexes: insights into miRNA uridylation

(2011) Kyu-Hyeon Yeom et al.   EMBO REPORTS

Probing cellular protein complexes using single-molecule pull-down

(2011) Ankur Jain et al.   NATURE

Ultrahigh-resolution optical trap with single-fluorophore sensitivity

(2011) Matthew J Comstock et al.   NATURE METHODS

Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity

(2011) H. Hwang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ordered and Dynamic Assembly of Single Spliceosomes

(2011) A. A. Hoskins et al.   SCIENCE

Single-Molecule Four-Color FRET

(2010) Jinwoo Lee  et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Real-time tRNA transit on single translating ribosomes at codon resolution

(2010) Sotaro Uemura et al.   NATURE

Single-molecule imaging reveals mechanisms of protein disruption by a DNA translocase

(2010) Ilya J. Finkelstein et al.   NATURE

Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments

(2010) Jan Lipfert et al.   NATURE METHODS

Preassembled zinc-finger arrays for rapid construction of ZFNs

(2010) Seokjoong Kim et al.   NATURE METHODS

Stretching Single Talin Rod Molecules Activates Vinculin Binding

(2009) A. del Rio et al.   SCIENCE

A nano-positioning system for macromolecular structural analysis

(2008) Adam Muschielok et al.   NATURE METHODS

Imaging individual mRNA molecules using multiple singly labeled probes

(2008) Arjun Raj et al.   NATURE METHODS

An Oxygen Scavenging System for Improvement of Dye Stability in Single-Molecule Fluorescence Experiments

(2007) Colin Echeverría Aitken et al.   BIOPHYSICAL JOURNAL