Article
Biochemistry & Molecular Biology
Peng Teng, Kaisa Cui, Surui Yao, Bojian Fei, Feng Ling, Chaoqun Li, Zhaohui Huang
Summary: In this study, researchers found that ME2 is highly expressed in human colorectal cancer (CRC) tissues and its knockdown inhibits CRC cell proliferation. They also discovered that SIRT5 can desuccinylate ME2, activating its enzymatic activity. Activated ME2 enhances mitochondrial respiration, supporting cell proliferation and tumor development. Additionally, the levels of succinylated ME2 and SIRT5 are associated with patient prognosis.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Chemistry, Multidisciplinary
Sophia A. Rauscher, Joseph Moran
Summary: This study demonstrates that hydrogen gas can drive a part of the reverse Krebs cycle without the involvement of enzymes, under mild conditions compatible with biological chemistry. Low catalytic amounts of nickel, platinum group metals, and even ground meteorites were found to promote this reductive chemistry. These findings provide further support for the hypothesis that geologically produced hydrogen and metal catalysts played a role in early metabolic networks.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Toxicology
Amin Gasmi, Massimiliano Peana, Maria Arshad, Monica Butnariu, Alain Menzel, Geir Bjorklund
Summary: Cancerous tissues exhibit high glucose consumption, minimal oxidative metabolism and increased lactate concentrations. While glycolysis is a common feature of malignant cells, different tumors may have distinct metabolic characteristics.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rafael Franco, Joan Serrano-Marin
Summary: The Krebs cycle in cells plays a crucial role in both energy production and anabolic processes. This study compares closed and linear metabolism and finds that the closed cycle can reach a steady state regardless of disturbances, unlike the linear metabolism. Modeling the cycle in different conditions, including cancer, reveals that reductions in flux are limited, suggesting the presence of an undiscovered regulation mechanism. The findings have important implications for evaluating metabolism-modifying medicines.
Article
Multidisciplinary Sciences
Frits H. A. van Heijster, Vincent Breukels, Kees (C) F. J. Jansen, Jack A. Schalken, Arend Heerschap
Summary: Glucose is identified as the main carbon source for secreted citrate in prostate epithelial cells. Glutamine contributes to positions in secreted citrate through oxidative Krebs cycle and reductive carboxylation routes, but does not contribute to its net synthesis. Approximately 21% of pyruvate in the Krebs cycle is converted via pyruvate carboxylase as an anaplerotic route, compensating for carbon loss from citrate secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Genetics & Heredity
Chiara Ticci, Claudia Nesti, Anna Rubegni, Stefano Doccini, Jacopo Baldacci, Flavio Dal Canto, Luca Ragni, Duccio M. Cordelli, Maria Alice Donati, Filippo M. Santorelli
Summary: Bi-allelic alterations in the MDH2 gene have been found in three unrelated toddlers, while a new case with novel variants in MDH2 is described in this study. The patient presented with early-onset encephalocardiopathy, cerebellar ataxia, and drug-responsive epilepsy, expanding the spectrum of clinical features associated with MDH2 variants. Functional studies confirmed the genetic results, showing reduced protein levels and impaired enzyme activity in cultured skin fibroblasts from the proband.
Article
Agriculture, Multidisciplinary
Xin He, Shengjie Pei, Xiangyuan Meng, Qinglian Hua, Tianyu Zhang, Yan Wang, Zhizhao Zhang, Xinyu Zhu, Run Liu, Yurong Guo, Lei Chen, Duo Li
Summary: This study found that punicalagin inhibits diabetes-related neuronal apoptosis by upregulating 5hmC levels through activating AMPK and maintaining Krebs cycle homeostasis in the diabetic mouse brain.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Microbiology
Lee P. Hutt, Glenn M. Harper, A. John Moody, Rich Boden
Summary: The study demonstrated that Achromobacter aegrifaciens NCCB 38021 exhibited differences in growth characteristics under different growth conditions, with thiosulfate as an auxiliary electron donor significantly increasing growth rate. Thiosulfate oxidation was coupled to respiration at cytochrome c(551), leading to ATP generation.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Ahmed Elshobaky, Cathrine Lillo, Kristian Persson Hoden, Amr R. A. Kataya
Summary: Protein phosphatase 2A (PP2A) is a conserved serine/threonine phosphatase complex that plays a role in substrate specificity and localization in plants. In this study, the interactions between the PP2A-B'& zeta; subunit and enzymes related to mitochondrial energy flow were investigated. Through experiments in tobacco and onion cells, interactions were confirmed between the B'& zeta; subunit and several Krebs cycle proteins. Putative interacting candidates were also deduced from phosphoproteome analysis, shedding light on the role of PP2A in regulating energy metabolism in mitochondria.
Review
Medicine, Research & Experimental
Preeti Ranjan, Vikash Kumar Dubey
Summary: Leishmaniasis is a parasitic disease that disproportionately affects impoverished and developing countries. The socioeconomic background of the affected region is a significant factor that influences the severity of the disease. The emergence of drug-resistant Leishmania poses a serious threat to current treatment options, necessitating a reevaluation of drug choices. Targeted therapy combined with immunotherapy appears to be a promising strategy for combating the disease.
Review
Biology
Inseok Choi, Hyewon Son, Jea-Hyun Baek
Summary: The TCA cycle is a series of chemical reactions that occur in the mitochondria of aerobic organisms to generate energy from acetyl-CoA. The intermediates of the TCA cycle may leak from dysfunctional mitochondria and regulate immune responses under cell stress conditions. Increasing evidence suggests that the metabolites of the TCA cycle play important roles in the regulation of immune activation and suppression.
Article
Cell Biology
Rafael Aguiar Marschner, Ana Cristina Roginski, Rafael Teixeira Ribeiro, Larisse Longo, Mario Reis Alvares-da-Silva, Simone Magagnin Wajner
Summary: Metabolic dysfunction-associated fatty liver disease (MAFLD) is a global public health problem, but effective treatments are limited due to lack of mechanistic knowledge. This study evaluated the role of type 3 deiodinase (D3) in the progression of MAFLD using an animal model. The findings suggest that induced D3 decreases T3 availability and hepatic mitochondrial capacity, leading to alterations in Krebs cycle enzymes and endoplasmic reticulum stress.
Review
Endocrinology & Metabolism
Adil Rasheed, Katey J. Rayner
Summary: Macrophages are a heterogeneous population involved in tissue immunity, with their metabolism and environment-dependent identities regulating their response to external stimuli. The establishment of immunometabolism as a research field has highlighted the interaction between altered metabolism and cellular immunity, with macrophages playing a crucial role in disease progression through immune responses influenced by metabolic changes.
Article
Pharmacology & Pharmacy
Malak A. Jaber, Bayan Y. Ghanim, Mohammad Al-Natour, Duaa Abu Arqoub, Qasem Abdallah, Salah Abdelrazig, Jamal Alyousse Alkrad, Dong-Hyun Kim, Nidal A. Qinna
Summary: Alcohol consumption has significant toxic effects on population health worldwide, and the concurrent intake of Acetaminophen and alcohol is a concern in clinical settings. Understanding the molecular mechanisms behind this synergistic toxicity and acute effects can be improved through analyzing metabolomics changes. This study assessed the molecular toxic activities using metabolomics profiling to identify potential biomarkers and significant variables that could aid in managing drug-alcohol interactions.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2023)
Article
Materials Science, Ceramics
Justyna Zygmuntowicz, Paulina Piotrkiewicz, Radoslaw Zurowski, Anna Wieclaw-Midor, Pawel Falkowski, Marcin Wachowski, Justyna Tomaszewska
Summary: The Centrifugal Slip Casting method can be used to obtain a finished product from the Al2O3/Ti/Ni system. The content of the metallic phase does not affect the rheological properties, but it does affect the density and grain growth of the composites. The formation of spinel phase is possible using an air atmosphere. The hardness test confirms the gradient microstructure of the composites. Increasing the share of Ti and Ni phases leads to an increase in the environmental footprint of the materials produced.
CERAMICS INTERNATIONAL
(2023)
